Standard name
Human Ortholog
Description Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.56 0.54 0.56 0.55 0.59 0.8 0.84 0.84 – 0.86 0.86 0.87 0.47 0.65 0.86 0.86 0.78 0.67 0 0.12 0 0.14 0.16 0.2
Endoplasmic Reticulum 0.39 0.42 0.28 0.36 0.3 0.08 0 0 – 0 0 0 0.48 0.32 0.14 0 0 0 0.72 0.59 0.77 0.53 0.54 0.45
Endosome 0 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.08 0.06 – 0.09 0.12 0.09 0 0 0 0.05 0.06 0.06 0 0 0 0.08 0.06 0.06
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.15 0.07 0.14 0.08 0.17 0.14 0.14 0.12 – 0.13 0.08 0.1 0.09 0 0 0.05 0.12 0.26 0.17 0.16 0.07 0.16 0.15 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 3 3 7 7 0 1 0 – 0 1 3 7 0 1 6 2 1 2 4 6 4 9 10
Bud 0 2 1 7 4 0 5 8 – 9 9 22 0 1 1 0 0 0 0 2 0 4 7 8
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 1 3 3 0 4 3
Bud Site 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 4 5 2 7 1 2 2 – 2 5 6 17 12 8 3 5 1 0 0 1 0 1 2
Cytoplasm 92 144 172 124 182 346 523 605 – 389 357 455 143 168 278 132 124 58 10 35 16 35 67 86
Endoplasmic Reticulum 64 111 87 81 92 36 20 8 – 12 13 6 148 83 44 6 2 3 181 181 296 132 229 195
Endosome 1 0 15 3 10 4 2 5 – 1 1 2 3 0 0 5 1 3 2 7 3 4 4 4
Golgi 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 1 3 3 0 0 3
Mitochondria 1 8 11 8 1 17 50 43 – 42 48 45 0 0 0 8 10 5 5 9 16 19 25 26
Nucleus 0 1 1 0 0 0 4 11 – 2 1 1 0 1 1 0 3 3 0 0 0 0 0 0
Nuclear Periphery 1 0 2 2 0 0 3 9 – 2 1 0 1 1 0 0 0 0 3 2 3 2 1 1
Nucleolus 0 0 0 0 0 0 0 5 – 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 0 – 0 1 0 0 0 0 1 1 0 0 1 0 1 1 3
Vac/Vac Membrane 25 18 44 17 53 62 85 83 – 57 33 51 29 11 6 8 19 23 42 49 27 40 64 78
Unique Cell Count 165 267 308 225 309 431 620 716 451 417 522 306 258 325 154 158 87 254 308 386 250 424 433
Labelled Cell Count 186 291 341 251 357 466 695 779 517 471 592 348 277 339 170 168 97 254 308 386 250 424 433


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 6.5 6.1 7.1 7.5 5.9 5.2 5.1 – 4.8 4.7 4.9 7.9 7.1 6.8 6.6 6.6 7.7 7.1 7.2 9.0
Std Deviation (1e-4) 1.0 1.1 1.2 1.3 1.5 1.3 1.2 1.1 – 0.9 1.1 1.0 1.3 1.0 1.2 1.6 1.5 1.8 1.3 1.7 5.1
Intensity Change (Log2) – – – 0.21 0.31 -0.04 -0.23 -0.24 – -0.35 -0.38 -0.32 0.37 0.23 0.16 0.12 0.12 0.34 0.23 0.25 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.6 0 0 0 – 0 0 0 2.6 2.1 0.7 0 0 0
Cytoplasm -0.2 0.8 7.2 9.4 9.8 – 9.4 8.9 10.1 -2.3 2.2 8.2 6.4 4.8 1.8
Endoplasmic Reticulum 1.9 0.4 -7.2 -11.2 -13.7 – 0 0 0 5.1 1.0 -4.6 -6.2 -7.0 -4.9
Endosome -2.2 -1.0 -3.3 -4.9 -4.4 – 0 0 0 -2.9 -3.6 -4.0 -0.8 -2.4 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 -2.9 0.3 2.6 1.6 – 0 0 0 -3.3 -3.1 -3.4 0.8 1.4 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -2.4 1.0 0 -0.2 -1.2 – -0.7 -2.8 -2.0 -1.8 -4.0 -5.8 -2.9 -0.7 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6828 2.8124 2.736 2.3506 2.2769 2.8869 3.6942 4.4942 3.9777 3.4336 3.3103 3.9754 3.8776 4.7444 4.176 3.4662 4.2668 4.5705
Actin 0.0226 0.0013 0.0054 0.0063 0.014 0.0014 0.0152 0.0006 0.0056 0.0288 0.0274 0.0037 0.0096 0.0004 0.0019 0.0009 0.0259 0.0009
Bud 0.0005 0.0005 0.0008 0.0003 0.0053 0.0001 0.0005 0.0005 0.0011 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0
Bud Neck 0.0069 0.0006 0.0019 0.0009 0.0142 0.0128 0.0043 0.0057 0.0014 0.0006 0.001 0.0012 0.0004 0.0017 0.0006 0.0007 0.0011 0.0017
Bud Periphery 0.0009 0.0003 0.0023 0.0004 0.011 0.0003 0.0018 0.0005 0.001 0.0002 0.0005 0.0001 0.0001 0.0001 0.0002 0.0003 0.0004 0.0001
Bud Site 0.0054 0.0085 0.0063 0.0036 0.0325 0.0005 0.0058 0.0088 0.0049 0.0004 0.0014 0.0002 0.0007 0.0119 0.0016 0.0003 0.0008 0.0005
Cell Periphery 0.0007 0.001 0.0045 0.0005 0.0006 0.0003 0.0007 0.001 0.0029 0.0004 0.0007 0.0003 0.0014 0.0012 0.0009 0.0011 0.0005 0.0003
Cytoplasm 0.3983 0.5291 0.4457 0.4408 0.4132 0.501 0.2747 0.3405 0.3381 0.349 0.4293 0.4118 0.3512 0.4467 0.3946 0.4471 0.5032 0.4018
Cytoplasmic Foci 0.0225 0.0189 0.0232 0.0219 0.0244 0.0212 0.0174 0.0086 0.01 0.0204 0.0228 0.0135 0.0153 0.0094 0.0096 0.0101 0.0191 0.0077
Eisosomes 0.0003 0.0001 0.0005 0.0002 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0013 0 0.0001 0.0001 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.1834 0.2023 0.2384 0.2494 0.1083 0.2349 0.324 0.4331 0.3537 0.3038 0.2337 0.3614 0.3749 0.3369 0.353 0.2442 0.266 0.3975
Endosome 0.0356 0.0311 0.0333 0.0493 0.032 0.0306 0.0296 0.0217 0.0168 0.0434 0.0432 0.0264 0.0253 0.023 0.0218 0.0222 0.0357 0.0147
Golgi 0.0045 0.0016 0.005 0.0052 0.0068 0.0021 0.0029 0.0007 0.001 0.004 0.0081 0.0016 0.0025 0.0008 0.0013 0.0006 0.0041 0.0006
Lipid Particles 0.0152 0.0042 0.0052 0.012 0.013 0.0102 0.0097 0.006 0.0056 0.0089 0.0133 0.0055 0.0162 0.0064 0.0043 0.0038 0.0066 0.0052
Mitochondria 0.0097 0.0024 0.0171 0.0062 0.0348 0.0082 0.0013 0.0005 0.0024 0.001 0.0043 0.0026 0.0005 0.0003 0.0004 0.0006 0.0015 0.0003
None 0.2329 0.1406 0.1653 0.1127 0.1998 0.1175 0.2572 0.1235 0.219 0.1779 0.1487 0.1241 0.1639 0.1231 0.1704 0.213 0.0932 0.1359
Nuclear Periphery 0.0234 0.0211 0.0202 0.0275 0.0247 0.0143 0.0262 0.0211 0.0164 0.0204 0.0233 0.0204 0.0167 0.0197 0.018 0.0324 0.0164 0.0154
Nucleolus 0.0014 0.0012 0.0007 0.0008 0.002 0.0011 0.0006 0.0008 0.0003 0.0003 0.0004 0.0003 0.0002 0.0002 0.0002 0.0004 0.0002 0.0004
Nucleus 0.0088 0.0151 0.0085 0.0287 0.0226 0.0191 0.0087 0.0088 0.0059 0.0087 0.0093 0.0096 0.0041 0.0052 0.0047 0.007 0.0041 0.0056
Peroxisomes 0.005 0.0007 0.0015 0.0081 0.024 0.0034 0.0027 0.0001 0.0013 0.0159 0.015 0.0026 0.005 0.0001 0.0003 0.0001 0.0051 0.0001
Punctate Nuclear 0.0091 0.0049 0.0031 0.0039 0.0056 0.008 0.0036 0.0021 0.0031 0.0019 0.0031 0.0047 0.0021 0.001 0.0034 0.0038 0.0041 0.0026
Vacuole 0.0113 0.0135 0.0098 0.0179 0.0087 0.012 0.0117 0.0146 0.0083 0.0125 0.0112 0.009 0.0089 0.011 0.012 0.0099 0.0106 0.0082
Vacuole Periphery 0.0016 0.0012 0.0013 0.0034 0.0022 0.0009 0.0013 0.0008 0.0008 0.0011 0.0016 0.0011 0.0007 0.0009 0.0007 0.0012 0.001 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 55.0579 42.8568 29.3171 46.4698 42.7338 47.1663 47.9027 48.8965 50.3315 59.5785
Translational Efficiency 0.7565 0.6017 0.9681 0.5209 0.572 1.0074 0.8022 0.5764 0.7188 0.5969

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
Localization
Cell Percentages ER (85%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Avt7

Avt7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Avt7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available