Standard name
Human Ortholog
Description Ser/Thr protein kinase; regulates the organization and function of the actin cytoskeleton; inhibits clathrin-mediated endocytosis; phosphorylates the Pan1p and Sla1p subunits of the Pan1p-Sla1p-End3p complex, resulting in inhibition of complex formation; inhibits Scd5p through phosphorylation; phosphorylates Pan1p-interacting proteins, Ent1/2 and Yap1801/2; negatively regulated through autophosphorylation; functional overlap with ARK1

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.44 0.38 0.22 0.25 0.14 0.08 0.14 0.09 0.07 0 0.41 0.4 0.18 0.42 0.36 0.33 0.6 0.49 0.29 0.38 0.47 0.59
Bud 0.18 0.19 0.09 0.16 0.24 0.09 0.15 0.06 0.09 0.17 0.2 0.14 0.16 0.16 0.15 0.15 0 0.06 0 0.06 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0
Bud Site 0 0 0 0 0.07 0 0 0.09 0.06 0.05 0.07 0 0 0 0 0.05
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.13 0.13 0.29 0.05 0.24 0.27 0.22 0.16 0.12 0.08 0.16 0.18 0.14 0.18 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0.06 0 0 0.05 0.05 0 0 0 0 0 0.09 0.11 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0.17 0.05 0 0.05 0.07 0 0 0.07 0 0 0 0.06 0.06 0.08 0.05 0.06 0.12 0.07 0.07 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.1 0.05 0 0.06 0 0 0 0
Mitochondria 0.6 0.54 0.52 0.11 0.62 0.52 0.44 0.69 0.72 0.65 0.57 0.26 0.26 0.11 0.1 0.11 0 0.09 0.08 0.08 0.12 0.07
Nucleus 0 0 0 0 0.1 0 0 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.29 0.21 0.13 0.2 0.14 0.13 0.11 0.06 0 0.05 0.21 0.31 0.3 0.18 0.19 0.17 0.21 0.14 0.29 0.27 0.16 0.14
SpindlePole 0 0 0 0.18 0.1 0.08 0.13 0.06 0 0.1 0 0 0 0.27 0.26 0.33 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 88 93 5 14 6 12 22 6 5 1 50 49 30 194 154 128 142 139 17 77 85 108
Bud 36 46 2 9 10 13 23 4 6 7 25 17 27 72 64 59 7 16 1 12 9 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 3 15 1 4 6 2 11 11 3
Bud Site 8 5 1 1 3 4 6 6 4 2 8 1 0 17 9 19
Cell Periphery 4 7 1 0 0 2 1 0 1 0 1 7 10 8 7 5 0 0 0 0 1 2
Cytoplasm 9 33 3 16 2 35 42 15 11 5 10 19 31 64 77 57 1 7 0 1 1 1
Endoplasmic Reticulum 11 15 1 0 2 7 2 2 0 0 2 11 19 26 19 8 1 0 0 1 2 3
Endosome 7 5 4 3 0 7 11 2 0 3 1 3 6 26 25 29 11 16 6 14 13 7
Golgi 9 7 0 0 0 1 1 0 0 0 3 3 13 29 41 21 8 16 0 4 2 6
Mitochondria 120 134 12 6 26 74 70 47 50 26 70 32 44 50 42 42 8 25 4 16 21 13
Nucleus 1 0 1 0 4 3 3 4 5 0 0 0 0 3 9 3 0 0 0 0 0 0
Nuclear Periphery 1 1 0 0 0 0 0 0 0 0 2 3 0 3 3 4 0 1 0 0 0 0
Nucleolus 1 1 0 0 1 0 1 0 0 1 1 1 0 0 0 1 0 0 0 0 0 0
Peroxisomes 59 51 3 11 6 19 18 4 3 2 26 38 50 83 81 65 49 39 17 54 28 24
SpindlePole 8 2 0 10 4 12 20 4 2 4 1 1 3 126 110 126 4 4 2 4 3 1
Vac/Vac Membrane 2 3 0 0 0 0 5 2 2 0 0 0 0 5 3 6 0 4 2 0 0 0
Unique Cell Count 201 247 23 56 42 143 158 68 69 40 123 121 168 462 428 384 239 283 59 203 183 183
Labelled Cell Count 364 403 33 70 64 189 225 96 89 51 200 185 233 709 659 574 239 283 59 203 183 183


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 5.8 5.7 6.7 4.8 5.9 5.9 5.0 4.9 4.6 5.7 6.1 6.2 7.5 7.8 7.0 5.7 5.9 6.2
Std Deviation (1e-4) 1.0 1.0 1.2 1.7 1.0 1.9 1.5 1.6 1.4 1.2 0.9 1.1 1.0 1.4 1.6 1.3 1.3 1.4 1.5
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.8852 3.6251 3.5365 3.1615 3.1067 3.2981 1.4397 1.2437 1.2597 1.3916 1.2898 1.3151 1.3881 1.4545 1.2118 1.465 1.3879 1.4009
Actin 0.4648 0.6892 0.78 0.6075 0.7288 0.8158 0.6431 0.6983 0.7927 0.7011 0.7346 0.8148 0.6251 0.5258 0.6968 0.5373 0.9299 0.7172
Bud 0.0037 0.0027 0.0063 0.0201 0.0006 0.0005 0.0021 0.0047 0.028 0.0173 0.0139 0.0006 0.0027 0.0045 0.0142 0.0343 0.001 0.0009
Bud Neck 0.0381 0.0056 0.0142 0.0025 0.0006 0.0325 0.0072 0.0041 0.0076 0.0011 0.0008 0.0678 0.0051 0.005 0.0259 0.0202 0.0013 0.0566
Bud Periphery 0.0072 0.0033 0.0197 0.0451 0.0009 0.0007 0.0035 0.0095 0.0501 0.0818 0.034 0.0009 0.0059 0.0052 0.0383 0.1054 0.0013 0.0013
Bud Site 0.0564 0.1339 0.0689 0.0219 0.0022 0.0018 0.0201 0.0862 0.0382 0.0035 0.0126 0.0014 0.028 0.1144 0.0376 0.0096 0.0026 0.0026
Cell Periphery 0.0028 0.0012 0.0015 0.0009 0.0001 0.0002 0.0006 0.0006 0.0005 0.0004 0.0008 0.0002 0.0015 0.0006 0.0014 0.001 0.0001 0.0004
Cytoplasm 0.0152 0.0404 0.0058 0.0354 0.0039 0.0087 0.0159 0.0384 0.0115 0.0384 0.0189 0.006 0.0463 0.0721 0.0299 0.0147 0.0003 0.0076
Cytoplasmic Foci 0.0837 0.0455 0.0163 0.0511 0.0282 0.0285 0.0652 0.0313 0.0126 0.0212 0.0278 0.0187 0.0576 0.0947 0.0241 0.0939 0.0098 0.0559
Eisosomes 0.0036 0.0013 0.0016 0.0008 0.0004 0.0026 0.0007 0.0006 0.0004 0.0004 0.0003 0.0005 0.001 0.0007 0.0013 0.0005 0.0005 0.0005
Endoplasmic Reticulum 0.0121 0.0007 0.0144 0.0019 0.0011 0.0006 0.0076 0.0012 0.0009 0.0006 0.0053 0.0004 0.0055 0.0014 0.0009 0.0042 0.0001 0.0009
Endosome 0.0299 0.0031 0.0022 0.0252 0.0235 0.0121 0.0458 0.0105 0.0073 0.0232 0.049 0.0069 0.0239 0.0181 0.0075 0.0192 0.0007 0.0099
Golgi 0.0198 0.0099 0.0052 0.0203 0.0054 0.0258 0.0612 0.0153 0.0088 0.021 0.0206 0.0137 0.0313 0.0413 0.0113 0.0194 0.0068 0.0179
Lipid Particles 0.0232 0.0019 0.0015 0.0071 0.0087 0.0091 0.0221 0.0021 0.0021 0.0007 0.0036 0.0015 0.0112 0.0033 0.0045 0.0097 0.001 0.0061
Mitochondria 0.0333 0.0014 0.0049 0.0195 0.0013 0.0038 0.0096 0.012 0.0098 0.008 0.0144 0.0113 0.0073 0.0034 0.0093 0.0076 0.0014 0.005
None 0.0263 0.0174 0.0135 0.0137 0.005 0.0014 0.0044 0.0209 0.001 0.0259 0.0056 0.0008 0.0126 0.0467 0.0314 0.0354 0.0001 0.0155
Nuclear Periphery 0.0121 0.0001 0.0033 0.0008 0.018 0.0008 0.0085 0.0002 0.0015 0.0005 0.0045 0.0076 0.0082 0.0002 0.0072 0.0329 0 0.0002
Nucleolus 0.0028 0.0002 0.0001 0.0002 0.0012 0.0001 0.0008 0.0001 0.0001 0.0001 0.0004 0 0.0003 0.0001 0.0003 0.0003 0 0.0001
Nucleus 0.0198 0.0002 0.0006 0.0006 0.0043 0.0006 0.0043 0.0001 0.0021 0.0003 0.0018 0.001 0.0024 0.0002 0.0015 0.0038 0 0.0003
Peroxisomes 0.1095 0.0412 0.0381 0.12 0.1041 0.0523 0.0668 0.0626 0.0232 0.0488 0.0392 0.0445 0.1167 0.0605 0.0518 0.045 0.0431 0.0993
Punctate Nuclear 0.0307 0.0006 0.0003 0.0013 0.0604 0.0018 0.0066 0.0004 0.0003 0.0003 0.0006 0.0004 0.0036 0.0013 0.0038 0.0024 0.0001 0.0008
Vacuole 0.0034 0.0002 0.001 0.0025 0.0006 0.0003 0.0023 0.0005 0.0008 0.0041 0.0091 0.0005 0.0028 0.0005 0.0006 0.0017 0 0.0007
Vacuole Periphery 0.0015 0.0001 0.0005 0.0015 0.0006 0.0003 0.0014 0.0003 0.0003 0.0012 0.0022 0.0005 0.001 0.0002 0.0003 0.0017 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.8436 7.7029 9.3259 11.7955 7.056 9.9718 10.8628 11.3433 12.4199 12.3208
Translational Efficiency 1.1947 1.2704 0.9831 0.6687 1.0726 1.1854 0.9384 0.8631 0.8326 0.8822

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Ser/Thr protein kinase; regulates the organization and function of the actin cytoskeleton; inhibits clathrin-mediated endocytosis; phosphorylates the Pan1p and Sla1p subunits of the Pan1p-Sla1p-End3p complex, resulting in inhibition of complex formation; inhibits Scd5p through phosphorylation; phosphorylates Pan1p-interacting proteins, Ent1/2 and Yap1801/2; negatively regulated through autophosphorylation; functional overlap with ARK1
Localization
Cell Percentages actin (56%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Prk1

Prk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available