Standard name | |
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Human Ortholog | |
Description | Ser/Thr protein kinase; regulates the organization and function of the actin cytoskeleton; inhibits clathrin-mediated endocytosis; phosphorylates the Pan1p and Sla1p subunits of the Pan1p-Sla1p-End3p complex, resulting in inhibition of complex formation; inhibits Scd5p through phosphorylation; phosphorylates Pan1p-interacting proteins, Ent1/2 and Yap1801/2; negatively regulated through autophosphorylation; functional overlap with ARK1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 88 | 93 | – | 5 | 14 | 6 | 12 | 22 | 6 | 5 | 1 | – | 50 | 49 | 30 | 194 | 154 | 128 | 142 | 139 | 17 | 77 | 85 | 108 |
Bud | 36 | 46 | – | 2 | 9 | 10 | 13 | 23 | 4 | 6 | 7 | – | 25 | 17 | 27 | 72 | 64 | 59 | 7 | 16 | 1 | 12 | 9 | 7 |
Bud Neck | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3 | 15 | 1 | 4 | 6 | 2 | 11 | 11 | 3 |
Bud Site | 8 | 5 | – | 1 | 1 | 3 | 4 | 6 | 6 | 4 | 2 | – | 8 | 1 | 0 | 17 | 9 | 19 | – | – | – | – | – | – |
Cell Periphery | 4 | 7 | – | 1 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | – | 1 | 7 | 10 | 8 | 7 | 5 | 0 | 0 | 0 | 0 | 1 | 2 |
Cytoplasm | 9 | 33 | – | 3 | 16 | 2 | 35 | 42 | 15 | 11 | 5 | – | 10 | 19 | 31 | 64 | 77 | 57 | 1 | 7 | 0 | 1 | 1 | 1 |
Endoplasmic Reticulum | 11 | 15 | – | 1 | 0 | 2 | 7 | 2 | 2 | 0 | 0 | – | 2 | 11 | 19 | 26 | 19 | 8 | 1 | 0 | 0 | 1 | 2 | 3 |
Endosome | 7 | 5 | – | 4 | 3 | 0 | 7 | 11 | 2 | 0 | 3 | – | 1 | 3 | 6 | 26 | 25 | 29 | 11 | 16 | 6 | 14 | 13 | 7 |
Golgi | 9 | 7 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | – | 3 | 3 | 13 | 29 | 41 | 21 | 8 | 16 | 0 | 4 | 2 | 6 |
Mitochondria | 120 | 134 | – | 12 | 6 | 26 | 74 | 70 | 47 | 50 | 26 | – | 70 | 32 | 44 | 50 | 42 | 42 | 8 | 25 | 4 | 16 | 21 | 13 |
Nucleus | 1 | 0 | – | 1 | 0 | 4 | 3 | 3 | 4 | 5 | 0 | – | 0 | 0 | 0 | 3 | 9 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 3 | 0 | 3 | 3 | 4 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 1 | – | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | – | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 59 | 51 | – | 3 | 11 | 6 | 19 | 18 | 4 | 3 | 2 | – | 26 | 38 | 50 | 83 | 81 | 65 | 49 | 39 | 17 | 54 | 28 | 24 |
SpindlePole | 8 | 2 | – | 0 | 10 | 4 | 12 | 20 | 4 | 2 | 4 | – | 1 | 1 | 3 | 126 | 110 | 126 | 4 | 4 | 2 | 4 | 3 | 1 |
Vac/Vac Membrane | 2 | 3 | – | 0 | 0 | 0 | 0 | 5 | 2 | 2 | 0 | – | 0 | 0 | 0 | 5 | 3 | 6 | 0 | 4 | 2 | 0 | 0 | 0 |
Unique Cell Count | 201 | 247 | 23 | 56 | 42 | 143 | 158 | 68 | 69 | 40 | 123 | 121 | 168 | 462 | 428 | 384 | 239 | 283 | 59 | 203 | 183 | 183 | ||
Labelled Cell Count | 364 | 403 | 33 | 70 | 64 | 189 | 225 | 96 | 89 | 51 | 200 | 185 | 233 | 709 | 659 | 574 | 239 | 283 | 59 | 203 | 183 | 183 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.5 | 5.8 | – | 5.7 | 6.7 | 4.8 | 5.9 | 5.9 | 5.0 | 4.9 | 4.6 | – | 5.7 | 6.1 | 6.2 | 7.5 | 7.8 | 7.0 | 5.7 | 5.9 | 6.2 |
Std Deviation (1e-4) | 1.0 | 1.0 | – | 1.2 | 1.7 | 1.0 | 1.9 | 1.5 | 1.6 | 1.4 | 1.2 | – | 0.9 | 1.1 | 1.0 | 1.4 | 1.6 | 1.3 | 1.3 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.8852 | 3.6251 | 3.5365 | 3.1615 | 3.1067 | 3.2981 | 1.4397 | 1.2437 | 1.2597 | 1.3916 | 1.2898 | 1.3151 | 1.3881 | 1.4545 | 1.2118 | 1.465 | 1.3879 | 1.4009 |
Actin | 0.4648 | 0.6892 | 0.78 | 0.6075 | 0.7288 | 0.8158 | 0.6431 | 0.6983 | 0.7927 | 0.7011 | 0.7346 | 0.8148 | 0.6251 | 0.5258 | 0.6968 | 0.5373 | 0.9299 | 0.7172 |
Bud | 0.0037 | 0.0027 | 0.0063 | 0.0201 | 0.0006 | 0.0005 | 0.0021 | 0.0047 | 0.028 | 0.0173 | 0.0139 | 0.0006 | 0.0027 | 0.0045 | 0.0142 | 0.0343 | 0.001 | 0.0009 |
Bud Neck | 0.0381 | 0.0056 | 0.0142 | 0.0025 | 0.0006 | 0.0325 | 0.0072 | 0.0041 | 0.0076 | 0.0011 | 0.0008 | 0.0678 | 0.0051 | 0.005 | 0.0259 | 0.0202 | 0.0013 | 0.0566 |
Bud Periphery | 0.0072 | 0.0033 | 0.0197 | 0.0451 | 0.0009 | 0.0007 | 0.0035 | 0.0095 | 0.0501 | 0.0818 | 0.034 | 0.0009 | 0.0059 | 0.0052 | 0.0383 | 0.1054 | 0.0013 | 0.0013 |
Bud Site | 0.0564 | 0.1339 | 0.0689 | 0.0219 | 0.0022 | 0.0018 | 0.0201 | 0.0862 | 0.0382 | 0.0035 | 0.0126 | 0.0014 | 0.028 | 0.1144 | 0.0376 | 0.0096 | 0.0026 | 0.0026 |
Cell Periphery | 0.0028 | 0.0012 | 0.0015 | 0.0009 | 0.0001 | 0.0002 | 0.0006 | 0.0006 | 0.0005 | 0.0004 | 0.0008 | 0.0002 | 0.0015 | 0.0006 | 0.0014 | 0.001 | 0.0001 | 0.0004 |
Cytoplasm | 0.0152 | 0.0404 | 0.0058 | 0.0354 | 0.0039 | 0.0087 | 0.0159 | 0.0384 | 0.0115 | 0.0384 | 0.0189 | 0.006 | 0.0463 | 0.0721 | 0.0299 | 0.0147 | 0.0003 | 0.0076 |
Cytoplasmic Foci | 0.0837 | 0.0455 | 0.0163 | 0.0511 | 0.0282 | 0.0285 | 0.0652 | 0.0313 | 0.0126 | 0.0212 | 0.0278 | 0.0187 | 0.0576 | 0.0947 | 0.0241 | 0.0939 | 0.0098 | 0.0559 |
Eisosomes | 0.0036 | 0.0013 | 0.0016 | 0.0008 | 0.0004 | 0.0026 | 0.0007 | 0.0006 | 0.0004 | 0.0004 | 0.0003 | 0.0005 | 0.001 | 0.0007 | 0.0013 | 0.0005 | 0.0005 | 0.0005 |
Endoplasmic Reticulum | 0.0121 | 0.0007 | 0.0144 | 0.0019 | 0.0011 | 0.0006 | 0.0076 | 0.0012 | 0.0009 | 0.0006 | 0.0053 | 0.0004 | 0.0055 | 0.0014 | 0.0009 | 0.0042 | 0.0001 | 0.0009 |
Endosome | 0.0299 | 0.0031 | 0.0022 | 0.0252 | 0.0235 | 0.0121 | 0.0458 | 0.0105 | 0.0073 | 0.0232 | 0.049 | 0.0069 | 0.0239 | 0.0181 | 0.0075 | 0.0192 | 0.0007 | 0.0099 |
Golgi | 0.0198 | 0.0099 | 0.0052 | 0.0203 | 0.0054 | 0.0258 | 0.0612 | 0.0153 | 0.0088 | 0.021 | 0.0206 | 0.0137 | 0.0313 | 0.0413 | 0.0113 | 0.0194 | 0.0068 | 0.0179 |
Lipid Particles | 0.0232 | 0.0019 | 0.0015 | 0.0071 | 0.0087 | 0.0091 | 0.0221 | 0.0021 | 0.0021 | 0.0007 | 0.0036 | 0.0015 | 0.0112 | 0.0033 | 0.0045 | 0.0097 | 0.001 | 0.0061 |
Mitochondria | 0.0333 | 0.0014 | 0.0049 | 0.0195 | 0.0013 | 0.0038 | 0.0096 | 0.012 | 0.0098 | 0.008 | 0.0144 | 0.0113 | 0.0073 | 0.0034 | 0.0093 | 0.0076 | 0.0014 | 0.005 |
None | 0.0263 | 0.0174 | 0.0135 | 0.0137 | 0.005 | 0.0014 | 0.0044 | 0.0209 | 0.001 | 0.0259 | 0.0056 | 0.0008 | 0.0126 | 0.0467 | 0.0314 | 0.0354 | 0.0001 | 0.0155 |
Nuclear Periphery | 0.0121 | 0.0001 | 0.0033 | 0.0008 | 0.018 | 0.0008 | 0.0085 | 0.0002 | 0.0015 | 0.0005 | 0.0045 | 0.0076 | 0.0082 | 0.0002 | 0.0072 | 0.0329 | 0 | 0.0002 |
Nucleolus | 0.0028 | 0.0002 | 0.0001 | 0.0002 | 0.0012 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0003 | 0.0003 | 0 | 0.0001 |
Nucleus | 0.0198 | 0.0002 | 0.0006 | 0.0006 | 0.0043 | 0.0006 | 0.0043 | 0.0001 | 0.0021 | 0.0003 | 0.0018 | 0.001 | 0.0024 | 0.0002 | 0.0015 | 0.0038 | 0 | 0.0003 |
Peroxisomes | 0.1095 | 0.0412 | 0.0381 | 0.12 | 0.1041 | 0.0523 | 0.0668 | 0.0626 | 0.0232 | 0.0488 | 0.0392 | 0.0445 | 0.1167 | 0.0605 | 0.0518 | 0.045 | 0.0431 | 0.0993 |
Punctate Nuclear | 0.0307 | 0.0006 | 0.0003 | 0.0013 | 0.0604 | 0.0018 | 0.0066 | 0.0004 | 0.0003 | 0.0003 | 0.0006 | 0.0004 | 0.0036 | 0.0013 | 0.0038 | 0.0024 | 0.0001 | 0.0008 |
Vacuole | 0.0034 | 0.0002 | 0.001 | 0.0025 | 0.0006 | 0.0003 | 0.0023 | 0.0005 | 0.0008 | 0.0041 | 0.0091 | 0.0005 | 0.0028 | 0.0005 | 0.0006 | 0.0017 | 0 | 0.0007 |
Vacuole Periphery | 0.0015 | 0.0001 | 0.0005 | 0.0015 | 0.0006 | 0.0003 | 0.0014 | 0.0003 | 0.0003 | 0.0012 | 0.0022 | 0.0005 | 0.001 | 0.0002 | 0.0003 | 0.0017 | 0 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.8436 | 7.7029 | 9.3259 | 11.7955 | 7.056 | 9.9718 | 10.8628 | 11.3433 | 12.4199 | 12.3208 |
Translational Efficiency | 1.1947 | 1.2704 | 0.9831 | 0.6687 | 1.0726 | 1.1854 | 0.9384 | 0.8631 | 0.8326 | 0.8822 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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