Standard name
Human Ortholog
Description Mitochondrial matrix protein; required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.11 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.1 0 0 0 0 0 0
Cytoplasm 0 0.07 0.07 0.05 0 0 0 0 0 0 0 0.06 0.07 0.12 0.32 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0.06 0 0 0 0 0 0 0 0.1 0.09 0.22 0.08 0.13 0.08 0 0.08 0.06 0.08 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0.05 0.13 0 0.07 0 0 0.06 0.07
Mitochondria 0.83 0.92 0.94 0.89 0.94 0.92 0.94 0.94 0.97 0.97 0.88 0.81 0.67 0.09 0.18 0.1 0.76 0.72 0.73 0.71 0.69 0.66
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0.08 0.06 0.1 0.08 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.05 0.09 0.06 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.05 0 0 0 0 0 0 0 0.05 0.08 0.12 0.11 0.12 0 0 0 0.06 0.05 0.05
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.16 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0.22 0.05 0 0.05 0.06 0 0 0 0 0 0.1 0.09 0.12 0.22 0.16 0.24 0.1 0.08 0 0.05 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 3 1 0 0 0 0 0 0 0 5 4 0 3 3 9 0 3 3 2 3 7
Bud 2 8 10 10 14 33 23 30 37 31 4 4 0 2 0 5 0 0 2 1 0 1
Bud Neck 0 1 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 1 4
Bud Site 0 5 1 1 3 9 9 10 9 20 0 0 0 0 0 0
Cell Periphery 6 2 4 3 3 2 3 5 7 7 2 6 8 4 0 8 0 0 0 0 0 0
Cytoplasm 6 17 20 17 16 13 13 12 6 4 5 17 16 8 12 8 0 1 1 2 1 2
Endoplasmic Reticulum 1 5 2 1 3 6 2 1 5 3 4 8 6 1 1 2 0 1 0 1 0 0
Endosome 6 0 1 18 1 11 2 1 2 0 6 26 21 14 3 11 4 5 19 10 20 17
Golgi 7 0 2 0 0 1 0 0 0 0 0 7 11 7 2 11 1 12 2 6 15 19
Mitochondria 142 215 251 285 512 887 588 737 703 826 147 217 149 6 7 8 42 130 174 121 173 182
Nucleus 1 2 1 4 6 28 5 15 6 3 1 1 1 5 1 4 0 0 0 0 0 2
Nuclear Periphery 1 1 4 3 44 58 63 61 47 60 0 1 3 0 1 1 0 0 2 1 1 1
Nucleolus 0 0 0 6 28 85 40 42 31 51 1 2 1 0 0 1 0 0 1 1 1 2
Peroxisomes 2 0 0 16 3 21 6 6 6 8 7 13 17 8 4 10 0 6 10 10 11 13
SpindlePole 0 4 1 7 5 33 11 16 18 23 6 3 2 5 6 9 0 1 2 1 4 1
Vac/Vac Membrane 37 11 6 17 30 36 20 25 18 20 17 24 26 14 6 20 5 14 8 8 13 19
Unique Cell Count 171 234 267 319 543 965 626 782 722 852 167 269 223 64 38 84 56 182 239 172 252 278
Labelled Cell Count 212 274 304 388 668 1223 786 963 895 1056 205 333 261 77 46 107 56 182 239 172 252 278


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 3.6 4.0 5.0 4.2 4.4 4.1 4.3 4.2 4.2 4.8 5.6 5.5 6.6 5.9 6.3 5.4 5.6 5.6
Std Deviation (1e-4) 1.2 0.8 1.5 1.5 1.1 1.0 1.2 1.0 1.1 1.0 0.8 1.1 0.9 1.5 1.2 1.4 1.0 1.3 1.3
Intensity Change (Log2) 0.19 0.48 0.25 0.31 0.21 0.28 0.24 0.24 0.44 0.66 0.62 0.89 0.74 0.81 0.6 0.66 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 -0.2 0 0 0 0 0 0 0 -1.4 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 -0.9 -2.7 -5.2 -3.7 -4.6 -5.6 0 -1.9 -0.4 0 1.3 0 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0.6 0.4 0 0 0
Endosome 0 3.7 0 0 0 0 0 0 0 4.9 4.8 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 3.4 0 0 0
Mitochondria 0 -1.0 0 0 0 0 0 0 0 -3.6 -6.7 -13.4 -10.8 -14.1
Nucleus 0 0 0 1.8 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 3.5 0 0 0 0 0 0 0 3.4 4.3 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.5 0.3 0.5 -0.7 -1.1 -1.1 -1.7 -1.9 2.1 1.9 2.7 4.4 0 5.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3118 1.0849 0.9522 0.8733 0.3319 1.0144 0.6268 0.892 0.7151 0.6117 0.7435 0.5819 2.0556 2.0606 1.725 1.9771 1.2504 1.464
Actin 0.0483 0.009 0.0216 0.0053 0.0032 0.0195 0.0373 0.0069 0.0118 0.0302 0.0582 0.0128 0.0213 0.018 0.0122 0.0614 0.01 0.0039
Bud 0.0035 0.0016 0.0011 0.0008 0.0012 0.0007 0.004 0.0091 0.005 0.0067 0.0016 0.0006 0.0007 0.0028 0.0019 0.0027 0.0097 0.0005
Bud Neck 0.0037 0.0008 0.0016 0.0003 0.0008 0.0073 0.001 0.0013 0.0016 0.0024 0.0009 0.002 0.0011 0.0019 0.0021 0.0096 0.0014 0.0009
Bud Periphery 0.0049 0.002 0.0016 0.0019 0.0036 0.0014 0.0033 0.0094 0.0084 0.0157 0.0028 0.0018 0.0015 0.0042 0.0061 0.0119 0.0075 0.0017
Bud Site 0.0098 0.0174 0.0068 0.0006 0.001 0.0011 0.0028 0.04 0.0144 0.0077 0.0041 0.0008 0.0034 0.0309 0.0122 0.0042 0.0023 0.0007
Cell Periphery 0.0024 0.0005 0.0009 0.0002 0.0007 0.0003 0.0006 0.0026 0.0012 0.0015 0.0002 0.0004 0.0011 0.0013 0.0019 0.002 0.0005 0.0005
Cytoplasm 0.0122 0.0181 0.0159 0.0074 0.0033 0.0116 0.0236 0.0134 0.0061 0.0106 0.0011 0.0167 0.0158 0.0177 0.0201 0.051 0.0125 0.0041
Cytoplasmic Foci 0.045 0.08 0.042 0.0272 0.0438 0.0524 0.0735 0.048 0.0463 0.0661 0.0564 0.0648 0.0621 0.0665 0.055 0.0638 0.0853 0.0373
Eisosomes 0.0007 0.0003 0.0008 0.0002 0.0003 0.0005 0.0007 0.0009 0.0005 0.0008 0.0004 0.0003 0.0011 0.0028 0.0007 0.0005 0.0005 0.0002
Endoplasmic Reticulum 0.0078 0.0038 0.002 0.001 0.0016 0.0027 0.0035 0.0014 0.0016 0.0005 0.0006 0.0024 0.0073 0.0016 0.0023 0.002 0.0009 0.0006
Endosome 0.0817 0.0964 0.083 0.1284 0.1863 0.0774 0.1393 0.0605 0.0539 0.0378 0.1033 0.0672 0.1207 0.0667 0.0717 0.0394 0.125 0.0594
Golgi 0.0515 0.0845 0.0501 0.0603 0.1385 0.0702 0.0833 0.0511 0.0424 0.0322 0.0866 0.054 0.0484 0.0462 0.0427 0.0466 0.1346 0.0517
Lipid Particles 0.032 0.024 0.0231 0.0043 0.0319 0.0325 0.0612 0.0202 0.0203 0.0044 0.0323 0.0169 0.0425 0.0207 0.0221 0.0037 0.0084 0.0229
Mitochondria 0.5808 0.5512 0.6403 0.6488 0.5497 0.6443 0.432 0.6482 0.6868 0.6871 0.5427 0.6347 0.5464 0.5377 0.6287 0.6201 0.4974 0.7095
None 0.0191 0.0293 0.0107 0.0536 0.0006 0.018 0.0321 0.0292 0.0205 0.0385 0.0007 0.0193 0.0327 0.0876 0.0393 0.036 0.0386 0.014
Nuclear Periphery 0.0163 0.0011 0.0028 0.0005 0.0021 0.0021 0.0087 0.0005 0.0019 0.0002 0.0041 0.0046 0.0082 0.0009 0.0018 0.0007 0.0015 0.0008
Nucleolus 0.0012 0.0008 0.0006 0.0002 0.0004 0.001 0.0017 0.0013 0.0018 0.0007 0.0004 0.0004 0.0013 0.0009 0.001 0.001 0.0015 0.0004
Nucleus 0.0068 0.0007 0.0013 0.0002 0.0006 0.0006 0.0028 0.0019 0.0021 0.0011 0.0005 0.0014 0.0204 0.0008 0.0018 0.0014 0.0013 0.0004
Peroxisomes 0.0489 0.0682 0.0768 0.0476 0.0124 0.0472 0.0667 0.0425 0.0563 0.043 0.0871 0.0908 0.0477 0.0819 0.0646 0.0267 0.0472 0.0837
Punctate Nuclear 0.0059 0.0019 0.002 0.0002 0.0003 0.0021 0.0112 0.0023 0.0052 0.0005 0.01 0.001 0.005 0.0006 0.0033 0.0039 0.0044 0.0005
Vacuole 0.01 0.0046 0.0095 0.0062 0.0092 0.0033 0.0063 0.007 0.0082 0.0088 0.0031 0.0039 0.0072 0.0061 0.0054 0.0087 0.0055 0.0034
Vacuole Periphery 0.0076 0.0035 0.0056 0.005 0.0084 0.0038 0.0044 0.0023 0.0039 0.0036 0.0029 0.0032 0.0043 0.0024 0.003 0.0028 0.0039 0.0029

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.3577 2.1076 14.0756 10.2732 9.1706 18.8512 13.8517 20.1363 23.2743 13.2868
Translational Efficiency 11.205 9.984 1.4646 2.635 2.744 1.7428 2.4027 1.8084 1.4289 2.6888

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Mitochondrial matrix protein; required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p
Localization
Cell Percentages mitochondrion (94%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Fmc1

Fmc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fmc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available