Standard name
Human Ortholog
Description Myosin-like protein associated with the nuclear envelope; nuclear basket protein that connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length; MLP2 has a paralog, MLP1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0.13 0.16 0.09 0.14 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0.1 0 0 0 0 0 0 0.07 0.16 0.18 0.31 0.22 0.24 0.14 0.26 0.13 0.13 0.22 0.23
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.61 0.84 0.82 0.63 0.81 0.73 0.84 0.8 0.85 0.86 0.26 0.24 0.17 0.08 0.17 0.13 0.05 0.13 0.07 0.06 0.07 0.08
Nucleus 0.05 0.16 0 0.08 0.05 0.08 0.1 0.09 0.09 0.07 0.1 0 0 0 0 0 0 0.08 0.06 0 0 0
Nuclear Periphery 0.49 0.23 0.42 0.34 0.35 0.22 0.25 0.2 0.21 0.2 0.51 0.51 0.47 0.35 0.26 0.26 0.64 0.32 0.54 0.52 0.47 0.45
Nucleolus 0.22 0 0.23 0.12 0.13 0.16 0.17 0.17 0.19 0.19 0.1 0.16 0.17 0.26 0.17 0.28 0 0 0.05 0.08 0.05 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0.12 0 0.06 0.05 0 0.06 0.06
Vac/Vac Membrane 0.15 0.06 0 0.08 0 0 0 0 0 0 0.26 0.14 0.19 0.31 0.24 0.21 0 0 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 3 6
Bud 0 2 2 2 1 15 9 17 11 19 1 0 2 0 0 0 1 0 0 1 6 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0
Bud Site 0 0 1 4 4 9 14 15 12 39 1 0 2 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 3 11 18 16 19 11 17 11 16 14 76 109 16 16 21 0 0 0 1 2 1
Endoplasmic Reticulum 1 2 3 0 1 0 0 0 0 2 1 1 0 4 4 5 0 0 0 0 0 1
Endosome 4 0 12 50 9 22 5 11 4 8 22 96 124 55 25 39 25 5 17 10 98 127
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 2 1 0 0 0 3 12
Mitochondria 89 112 315 300 339 500 482 531 559 659 84 137 119 14 19 21 8 2 9 5 28 45
Nucleus 8 22 15 37 21 57 55 59 62 57 32 14 13 3 3 3 6 1 8 2 9 14
Nuclear Periphery 71 31 160 161 147 153 146 130 139 153 167 297 324 61 30 41 115 6 75 42 206 245
Nucleolus 32 4 90 59 56 110 97 110 123 147 31 93 117 46 20 45 4 0 7 6 21 11
Peroxisomes 0 0 2 12 3 10 1 6 3 5 1 7 17 2 4 6 0 0 1 2 8 14
SpindlePole 2 2 8 15 3 28 18 24 16 21 4 8 26 11 12 19 6 1 7 3 26 30
Vac/Vac Membrane 22 8 14 40 16 22 18 5 21 15 84 80 132 55 28 34 5 0 6 3 12 21
Unique Cell Count 146 134 385 479 418 681 573 663 657 766 325 582 688 176 115 160 180 20 139 81 439 546
Labelled Cell Count 232 186 634 698 616 945 857 925 961 1141 442 809 985 267 162 236 180 20 139 81 439 546


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 3.9 4.5 5.0 4.1 4.2 3.6 3.8 3.8 3.6 6.6 6.7 6.4 9.1 9.7 9.2 5.6 6.5 6.8
Std Deviation (1e-4) 0.7 0.7 1.0 1.1 1.1 0.9 0.9 0.6 0.8 0.8 1.2 1.3 1.4 1.8 1.9 1.9 0.8 1.2 1.1
Intensity Change (Log2) 0.22 0.36 0.1 0.12 -0.1 -0.02 -0.03 -0.09 0.78 0.79 0.73 1.23 1.33 1.25 0.52 0.75 0.82


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 3.6 4.2 2.5 3.5 3.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 3.9 0 0 0 0 0 0 3.1 5.1 5.3 7.1 5.7 6.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -4.6 0 0 0 0 0 0 -11.4 -13.2 -15.6 -13.4 -10.6 -12.1
Nucleus -4.9 -3.0 -4.3 -2.9 -2.3 -2.6 -2.4 -3.4 -2.0 -6.7 -7.6 -4.7 -3.6 -4.5
Nuclear Periphery 0 2.3 0 0 0 0 0 0 5.6 5.8 5.1 2.2 0.5 0.5
Nucleolus 0 3.1 0 0 0 0 0 0 2.4 4.0 4.2 5.5 3.8 5.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 2.1 3.1 3.4
Vacuole -1.2 0.9 -1.1 0 0 0 0 0 4.8 2.5 3.7 5.5 4.1 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8756 2.7998 2.6313 2.5894 2.0412 2.5856 2.0895 2.5593 2.0834 1.7833 1.9549 2.1395 3.7904 4.7968 4.0961 4.0822 4.0827 4.3192
Actin 0.0052 0.0007 0.0119 0.0041 0.0238 0.0007 0.0135 0.0006 0.0025 0.0099 0.0017 0.0018 0.0201 0.0016 0.0014 0.01 0.0023 0.0013
Bud 0.0001 0 0.0002 0.0001 0.0001 0 0.0002 0 0.0001 0.0019 0.0001 0.0001 0.0001 0.0001 0 0.0005 0 0
Bud Neck 0.0002 0.0001 0.0002 0.0002 0.0003 0.0001 0.0002 0.0001 0.0003 0.0006 0.0005 0.0004 0.0003 0.0002 0.0001 0.0006 0.0003 0.0002
Bud Periphery 0.0002 0.0001 0.0003 0.0005 0.0001 0.0001 0.0003 0.0001 0.0003 0.0021 0.0004 0.0002 0.0003 0.0003 0.0002 0.0022 0.0002 0.0002
Bud Site 0.0003 0.0001 0.0003 0.0001 0.0002 0 0.0008 0.0001 0.0006 0.0024 0.0004 0.0002 0.0006 0.0003 0.0001 0.0009 0 0
Cell Periphery 0.0001 0 0.0001 0.0001 0 0 0.0001 0 0.0001 0.0002 0.0002 0 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001
Cytoplasm 0.0064 0.0003 0.0026 0.0002 0.0009 0.0001 0.0101 0.0002 0.0015 0.0071 0.0036 0.0005 0.0015 0.0005 0.0002 0.0005 0.0001 0.0001
Cytoplasmic Foci 0.0064 0.0007 0.0042 0.0018 0.002 0.0005 0.0057 0.0008 0.0047 0.022 0.0099 0.0012 0.0046 0.0012 0.001 0.0034 0.0022 0.0007
Eisosomes 0.0001 0 0.0001 0.0002 0.0001 0 0.0002 0 0.0001 0.0001 0.0001 0 0.0002 0.0001 0.0001 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.0024 0.0011 0.002 0.0013 0.0008 0.0006 0.0029 0.0008 0.0016 0.0053 0.002 0.001 0.0016 0.001 0.001 0.0026 0.0011 0.0015
Endosome 0.0141 0.0017 0.0087 0.0027 0.0077 0.003 0.0176 0.0012 0.0164 0.0282 0.0451 0.0065 0.0071 0.0022 0.0012 0.004 0.0033 0.0017
Golgi 0.0048 0.0011 0.0035 0.0023 0.0022 0.0021 0.0065 0.0006 0.0031 0.0182 0.0162 0.0024 0.0026 0.0015 0.0011 0.0039 0.0045 0.0013
Lipid Particles 0.0582 0.0264 0.0342 0.0295 0.0383 0.0255 0.0293 0.0146 0.0202 0.0191 0.0528 0.017 0.0208 0.0225 0.0154 0.0407 0.1139 0.0309
Mitochondria 0.0051 0.0011 0.0024 0.004 0.0017 0.0021 0.0034 0.0008 0.0071 0.0165 0.0035 0.0037 0.0015 0.0017 0.0021 0.0043 0.0014 0.0043
None 0.0034 0.0004 0.0059 0.0015 0.002 0.0003 0.0062 0.0003 0.0019 0.0015 0.001 0.0004 0.0027 0.0009 0.0007 0.0027 0.0007 0.0005
Nuclear Periphery 0.8349 0.882 0.8272 0.86 0.8766 0.9221 0.8117 0.83 0.8009 0.6338 0.7057 0.8953 0.8612 0.7877 0.8682 0.7813 0.7102 0.8863
Nucleolus 0.005 0.0076 0.0153 0.0069 0.0046 0.007 0.0103 0.0357 0.0184 0.0621 0.07 0.0172 0.0106 0.0334 0.0184 0.0432 0.0731 0.0077
Nucleus 0.0108 0.0214 0.0233 0.0085 0.0085 0.009 0.0393 0.0651 0.0649 0.0677 0.043 0.0232 0.0153 0.0832 0.0324 0.0242 0.0104 0.0177
Peroxisomes 0.0021 0.0003 0.0011 0.0011 0.0038 0.0003 0.0012 0.0002 0.001 0.0203 0.0013 0.0005 0.0015 0.0007 0.0005 0.0033 0.0009 0.0005
Punctate Nuclear 0.0276 0.0386 0.0405 0.0581 0.0152 0.0175 0.0245 0.0307 0.0374 0.0638 0.0289 0.0187 0.0356 0.0437 0.0374 0.051 0.0648 0.0329
Vacuole 0.0011 0.0006 0.0014 0.0006 0.0009 0.0008 0.0017 0.0009 0.0021 0.0055 0.0027 0.0006 0.0007 0.001 0.0006 0.001 0.0008 0.0007
Vacuole Periphery 0.0116 0.0155 0.0145 0.0163 0.0099 0.0083 0.0145 0.0171 0.0149 0.0117 0.0109 0.009 0.011 0.0161 0.0179 0.0193 0.0099 0.0114

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.5144 16.0482 19.1697 24.9795 18.7342 23.1937 24.4879 25.4426 23.5639 27.3875
Translational Efficiency 0.6158 0.6857 0.5528 0.4184 0.4567 0.3958 0.3797 0.3608 0.4256 0.3505

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Myosin-like protein associated with the nuclear envelope; nuclear basket protein that connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length; MLP2 has a paralog, MLP1, that arose from the whole genome duplication
Localization
Cell Percentages nuclear periphery (64%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mlp2

Mlp2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mlp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available