Standard name
Human Ortholog
Description Putative membrane glycoprotein; has strong similarity to Vth2p and Pep1p/Vps10p; may be involved in vacuolar protein sorting
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.58 0.64 0.69 0.76 0.83 0.89 – 0.85 0.81 0.77 0.74 0.73 0.76 0.81 0.82 – – – 0.15 0.16 0.2 0.17 0.16 0.16
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0.16 0.05 0.05 – – – 0 0 0 0 0 0
Endosome 0.31 0.23 0.19 0.11 0.08 0 – 0 0 0 0 0 0 0.06 0.08 – – – 0.48 0.49 0.39 0.39 0.38 0.36
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0.06 0 0.07 0 0.24 – 0.3 0.4 0.46 0.49 0.48 0 0 0 – – – 0.09 0.11 0.18 0.16 0.15 0.15
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0.05 0 0.15 0.05 0.05 0 – 0 0 0 0 0 0.06 0.05 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.08 0 0 0 0 – 0 0 0 0 0 0 0 0.05 – – – 0.16 0.11 0.12 0.15 0.2 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 4 0 0 0 0 – 0 0 0 0 0 5 5 2 – – – 14 25 4 6 8 9
Bud 4 13 4 1 3 4 – 8 6 8 9 7 3 1 0 – – – 8 11 0 5 15 10
Bud Neck 1 0 4 0 0 1 – 4 0 1 1 1 4 5 0 – – – 2 8 2 1 4 2
Bud Site 0 0 0 0 0 0 – 0 0 0 0 3 0 0 0 – – – – – – – – –
Cell Periphery 1 1 0 0 0 2 – 0 0 1 1 2 4 0 0 – – – 0 0 0 0 1 0
Cytoplasm 306 486 217 125 177 243 – 278 257 298 148 160 227 296 293 – – – 93 146 68 71 118 91
Endoplasmic Reticulum 5 27 1 1 0 0 – 2 0 0 0 0 48 19 18 – – – 7 8 5 8 13 16
Endosome 162 176 59 18 17 1 – 2 0 0 0 0 13 21 30 – – – 306 455 135 160 283 202
Golgi 4 3 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 9 7 2 4 8 2
Mitochondria 19 49 8 12 9 66 – 98 128 177 99 106 0 1 0 – – – 58 104 63 66 110 81
Nucleus 0 2 0 0 0 1 – 3 1 0 1 0 0 0 0 – – – 0 1 0 0 2 0
Nuclear Periphery 1 2 0 0 0 0 – 1 0 1 0 0 0 0 0 – – – 2 3 0 1 1 2
Nucleolus 3 1 1 0 0 1 – 1 1 1 0 0 0 0 0 – – – 0 0 1 0 0 0
Peroxisomes 6 0 6 3 2 0 – 0 0 0 0 0 0 0 1 – – – 2 2 1 0 2 1
SpindlePole 27 21 46 9 11 1 – 2 3 7 1 8 17 17 11 – – – 19 38 12 13 22 17
Vac/Vac Membrane 61 60 13 6 5 5 – 3 0 1 1 1 6 11 17 – – – 102 100 41 61 150 112
Unique Cell Count 525 765 316 164 214 272 328 317 388 201 219 298 365 358 642 930 348 412 753 563
Labelled Cell Count 600 845 359 175 224 325 402 396 495 261 288 327 376 372 642 930 348 412 753 563


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 4.8 4.3 4.0 3.7 3.1 – 3.1 2.8 2.6 2.6 2.6 5.4 5.7 6.1 4.3 4.9 – 5.8 5.6 5.9
Std Deviation (1e-4) 1.0 0.8 1.3 1.5 1.2 1.6 – 0.9 0.8 1.0 0.8 1.2 0.8 0.8 1.0 1.3 1.5 – 1.4 1.3 1.4
Intensity Change (Log2) – – – -0.09 -0.22 -0.47 – -0.48 -0.6 -0.74 -0.74 -0.73 0.34 0.43 0.5 0.01 0.18 – 0.44 0.39 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4973 1.2705 0.8017 0.7522 0.9211 1.1073 3.3038 3.7671 3.7976 3.8194 3.384 3.8835 4.4538 2.7358 2.1067 2.3253 2.6084 2.8623
Actin 0.0038 0.0019 0.0033 0.0022 0.0209 0.001 0.0048 0.0018 0.0082 0.0123 0.0024 0.0055 0.0001 0.0016 0.0006 0.0001 0.0001 0.0047
Bud 0.0007 0.0158 0.0037 0.0048 0.002 0.002 0.0077 0.0058 0.0044 0.0087 0.0086 0.0032 0.0001 0.0003 0.0002 0.0001 0.0001 0.0001
Bud Neck 0.0016 0.0011 0.0003 0.0009 0.0007 0.001 0.0021 0.0011 0.0023 0.0006 0.0017 0.0022 0.0001 0.0004 0.0001 0.0004 0.0002 0.001
Bud Periphery 0.0006 0.0036 0.0015 0.0042 0.0019 0.0007 0.0047 0.0087 0.0038 0.0215 0.0042 0.0025 0.0001 0.0004 0.0002 0.0001 0.0001 0.0001
Bud Site 0.003 0.0204 0.0016 0.0019 0.0011 0.0006 0.0075 0.0131 0.0096 0.0015 0.0017 0.0006 0.0009 0.0014 0.0003 0.0005 0.0001 0.0002
Cell Periphery 0.0003 0.0005 0.0002 0.0002 0.0003 0.0002 0.0005 0.0005 0.0005 0.0002 0.0005 0.0003 0.0006 0.0003 0.0001 0 0.0001 0.0001
Cytoplasm 0.1337 0.1671 0.1383 0.114 0.086 0.1717 0.0475 0.1087 0.0552 0.0442 0.0729 0.0725 0.0388 0.1326 0.0915 0.0728 0.2086 0.1113
Cytoplasmic Foci 0.1835 0.1932 0.1983 0.2138 0.1894 0.2106 0.2164 0.238 0.2331 0.1639 0.2172 0.2762 0.0268 0.1734 0.2017 0.198 0.1698 0.2323
Eisosomes 0.0001 0.0001 0.0001 0 0.0002 0 0.0002 0.0001 0.0002 0.0002 0.0002 0.0001 0.0005 0.0002 0.0001 0 0 0
Endoplasmic Reticulum 0.006 0.0049 0.005 0.0024 0.0038 0.0044 0.0039 0.0037 0.0022 0.0119 0.0037 0.0021 0.0034 0.0066 0.0054 0.0012 0.0056 0.0027
Endosome 0.3961 0.3333 0.3674 0.4228 0.4349 0.3688 0.3527 0.3448 0.3015 0.3605 0.342 0.2777 0.052 0.3163 0.3892 0.2729 0.4106 0.3508
Golgi 0.0195 0.015 0.0212 0.0156 0.0202 0.0221 0.0269 0.0169 0.0298 0.0285 0.0353 0.0191 0.0017 0.0075 0.0293 0.0158 0.0065 0.0246
Lipid Particles 0.0318 0.0116 0.0174 0.0157 0.0206 0.0457 0.0636 0.0318 0.0345 0.0467 0.053 0.0532 0.0302 0.0264 0.009 0.0171 0.0049 0.0421
Mitochondria 0.0052 0.0056 0.0064 0.0038 0.0126 0.0078 0.014 0.0082 0.0174 0.0131 0.0139 0.0069 0.0025 0.0022 0.0049 0.0005 0.001 0.0054
None 0.1673 0.1769 0.1902 0.1416 0.1701 0.1281 0.1909 0.1438 0.2189 0.2328 0.139 0.2179 0.7826 0.293 0.2053 0.3928 0.162 0.1844
Nuclear Periphery 0.0032 0.002 0.0027 0.0016 0.0043 0.0027 0.0053 0.0024 0.0018 0.006 0.0033 0.0015 0.0061 0.0034 0.0019 0.0006 0.0025 0.001
Nucleolus 0.0006 0.0017 0.0006 0.0008 0.0007 0.0004 0.0013 0.003 0.0021 0.001 0.0009 0.0007 0.006 0.001 0.0002 0.0004 0.0002 0.0002
Nucleus 0.0037 0.0057 0.002 0.002 0.0018 0.0015 0.0026 0.0043 0.0038 0.0019 0.0033 0.0013 0.0072 0.001 0.0005 0.0005 0.0007 0.0005
Peroxisomes 0.0117 0.0064 0.0182 0.0066 0.0117 0.0117 0.0249 0.0279 0.0496 0.0228 0.0785 0.0402 0.0009 0.0079 0.044 0.0122 0.001 0.0129
Punctate Nuclear 0.0054 0.0041 0.0022 0.0038 0.0048 0.002 0.005 0.004 0.0049 0.0035 0.0047 0.0043 0.0048 0.0014 0.0009 0.0043 0.001 0.0016
Vacuole 0.0186 0.0247 0.0156 0.0334 0.0091 0.014 0.0141 0.0265 0.0134 0.0127 0.0092 0.0098 0.0313 0.0196 0.0099 0.0086 0.021 0.0208
Vacuole Periphery 0.0034 0.0044 0.0041 0.0078 0.0028 0.003 0.0033 0.0047 0.0028 0.0056 0.0037 0.0023 0.0032 0.0031 0.0047 0.001 0.004 0.0032

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.5765 12.9396 18.1016 18.5898 16.1521 22.5302 21.0725 22.5823 22.1021 22.4936
Translational Efficiency 0.2248 0.232 0.1987 0.1776 0.2314 0.1608 0.1852 0.2229 0.1187 0.1904

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative membrane glycoprotein; has strong similarity to Vth2p and Pep1p/Vps10p; may be involved in vacuolar protein sorting
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Vth1

Vth1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vth1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available