Standard name
Human Ortholog
Description Adenylate cyclase; required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
Bud 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.09 – 0.07 0.11 0.08 0 0 0 0 0 0.09 0.05 0.14 0.16 0.16 0.28 0.33 0.29 0.05 0 0.1 0.12 0.14 0.12
Cytoplasm 0.97 – 0.94 0.95 0.94 0.92 0.85 0.74 0.73 0.72 0.5 0.52 0.98 0.99 0.99 0.88 0.84 0.85 0.81 0.8 0.6 0.6 0.62 0.63
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.05 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0.05 0.13 0.07 0.18 0.28 0.39 0.47 0.45 0.62 0.62 0 0 0 0 0 0 0 0.05 0.15 0.07 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 – 0 2 2 1 0 1 0 2 3 5 2 3 2 4 10 10 6 0 3 4 17 25
Bud 1 – 4 3 1 1 8 17 17 18 14 11 5 3 4 2 9 3 0 0 0 1 4 6
Bud Neck 0 – 1 0 0 1 4 1 2 1 0 0 0 0 1 0 1 0 5 0 3 6 10 15
Bud Site 0 – 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 21 – 7 20 25 15 15 22 17 12 25 18 38 70 78 65 122 87 11 1 10 13 47 46
Cytoplasm 226 – 90 178 290 312 454 396 285 313 137 184 267 439 487 201 311 259 194 39 64 69 208 255
Endoplasmic Reticulum 0 – 0 0 1 1 3 5 1 5 4 13 0 1 0 13 17 16 6 1 1 4 10 10
Endosome 0 – 0 0 1 0 0 0 0 0 0 2 1 0 0 0 3 1 0 0 0 1 3 4
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 1 2 0 1 3 1 0 0 0 3 2
Mitochondria 5 – 5 24 21 61 148 209 182 196 169 223 1 2 1 7 6 6 8 2 16 8 14 17
Nucleus 2 – 0 0 0 0 0 3 0 2 1 0 0 0 0 1 2 5 0 0 0 0 1 0
Nuclear Periphery 0 – 0 1 1 0 2 0 0 2 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Nucleolus 0 – 0 0 0 0 2 0 0 1 0 3 0 0 0 1 0 2 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 2 1 4 1 2 3 2 0 0 0 0 0 1 0 0 0 0 0 1
Vac/Vac Membrane 0 – 0 0 2 0 3 3 1 0 1 3 1 0 0 6 10 10 3 0 1 3 8 10
Unique Cell Count 232 96 187 307 340 533 535 388 433 273 357 272 444 494 229 369 305 240 49 107 117 337 404
Labelled Cell Count 257 107 228 344 394 640 661 506 556 357 465 315 519 575 300 493 403 240 49 107 117 337 404


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 – 3.7 3.9 4.5 3.7 3.5 3.6 3.2 3.6 3.3 3.4 5.6 5.8 5.7 8.3 8.0 7.8 5.0 6.0 6.1
Std Deviation (1e-4) 0.8 – 0.7 0.9 1.5 0.9 1.0 0.5 0.7 0.9 0.8 0.9 1.3 1.1 0.8 1.6 1.5 1.7 0.9 1.3 1.5
Intensity Change (Log2) – – – 0.08 0.28 -0.02 -0.07 -0.04 -0.21 -0.06 -0.17 -0.15 0.6 0.64 0.61 1.15 1.11 1.07 0.44 0.69 0.71


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 1.7 2.2 2.2 4.2 5.0 4.3
Cytoplasm 0.5 0.3 -0.6 -2.3 -4.2 -4.3 -4.5 -7.5 -7.5 2.2 3.2 3.0 -1.6 -2.4 -2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0451 1.1166 1.1155 0.585 0.5513 1.1964 1.3446 1.0518 0.8484 0.7561 1.3158 0.8523 2.4936 2.68 2.7053 2.1553 3.1602 2.5691
Actin 0.0896 0.0049 0.0308 0.0309 0.018 0.0332 0.0051 0.0033 0.0064 0.0019 0.1095 0.0103 0.0894 0.0055 0.0408 0.0016 0.0768 0.0136
Bud 0.0054 0.0082 0.0107 0.0003 0.0024 0.0061 0.0022 0.0036 0.0009 0.0005 0.0039 0.0013 0.002 0.0007 0.0052 0.0005 0.0006 0.0008
Bud Neck 0.026 0.0015 0.0038 0.0019 0.0158 0.0125 0.0038 0.0008 0.002 0.0021 0.0038 0.0046 0.0044 0.0005 0.0015 0.0002 0.0155 0.0023
Bud Periphery 0.0139 0.0071 0.0114 0.0005 0.0051 0.0063 0.0023 0.0041 0.0008 0.0005 0.0063 0.003 0.006 0.0014 0.0114 0.0015 0.0008 0.0014
Bud Site 0.0494 0.0695 0.0239 0.0007 0.0345 0.0045 0.0072 0.018 0.0111 0.0011 0.0288 0.0009 0.0036 0.0107 0.0032 0.0002 0.0086 0.0024
Cell Periphery 0.0122 0.0079 0.0052 0.0026 0.0027 0.0032 0.0024 0.0022 0.0013 0.0004 0.0018 0.0007 0.0019 0.0029 0.002 0.0011 0.0006 0.0015
Cytoplasm 0.2034 0.5887 0.6383 0.7824 0.6366 0.6394 0.4909 0.6157 0.6388 0.4817 0.5416 0.6229 0.287 0.6289 0.6213 0.5498 0.6631 0.7695
Cytoplasmic Foci 0.1132 0.0099 0.0123 0.0118 0.0054 0.0054 0.0923 0.0152 0.0197 0.0191 0.0409 0.0218 0.0814 0.0027 0.0111 0.0051 0.0329 0.0071
Eisosomes 0.0016 0.0006 0.0005 0.0002 0.0003 0.0004 0.0001 0.0004 0.0001 0.0001 0.0006 0.0001 0.0013 0.0001 0.0003 0.0002 0.0003 0.0001
Endoplasmic Reticulum 0.0057 0.0033 0.0034 0.0017 0.0039 0.0023 0.0088 0.0043 0.0029 0.0039 0.0048 0.0024 0.0115 0.0018 0.0021 0.0059 0.0016 0.0028
Endosome 0.064 0.0061 0.0063 0.0115 0.0016 0.0053 0.1332 0.0084 0.0203 0.0345 0.0189 0.0217 0.0403 0.0005 0.0014 0.0014 0.0084 0.0008
Golgi 0.0231 0.002 0.0025 0.0073 0.001 0.0021 0.025 0.0008 0.0125 0.0181 0.033 0.0079 0.0211 0.0004 0.0038 0.0006 0.0167 0.0019
Lipid Particles 0.0526 0.0012 0.0062 0.0142 0.0009 0.0031 0.0221 0.0009 0.0011 0.0016 0.0055 0.0096 0.0334 0.0002 0.003 0.0004 0.0148 0.0005
Mitochondria 0.005 0.0031 0.0009 0.0008 0.0002 0.0009 0.0062 0.0002 0.0074 0.0126 0.081 0.0062 0.0208 0.0001 0.0007 0.0001 0.0007 0.0006
None 0.1862 0.2785 0.2286 0.1186 0.2656 0.248 0.0784 0.3083 0.2549 0.4116 0.0815 0.2774 0.3148 0.3419 0.2765 0.4289 0.1362 0.19
Nuclear Periphery 0.0088 0.0004 0.0013 0.0025 0.0007 0.0076 0.0211 0.0006 0.0006 0.0007 0.0011 0.0005 0.0055 0.0002 0.0002 0.0006 0.0004 0.0002
Nucleolus 0.0048 0.0005 0.0007 0.0002 0.0004 0.0005 0.0017 0.0002 0.0001 0.0001 0.0007 0.0001 0.001 0.0001 0.0001 0.0001 0.0002 0
Nucleus 0.0078 0.0008 0.003 0.0004 0.0009 0.0053 0.0169 0.0008 0.0008 0.0009 0.0017 0.0006 0.0019 0.0003 0.0003 0.0004 0.0003 0.0003
Peroxisomes 0.0594 0.0011 0.0012 0.0049 0.0004 0.0066 0.0093 0.0006 0.0011 0.0025 0.0243 0.0043 0.0588 0.0001 0.0134 0.0001 0.0154 0.0036
Punctate Nuclear 0.0584 0.0009 0.007 0.0053 0.0022 0.0056 0.0162 0.0007 0.0004 0.0004 0.0031 0.0007 0.0067 0.0001 0.0008 0.0004 0.0056 0.0003
Vacuole 0.0084 0.0032 0.0019 0.001 0.0012 0.0015 0.0469 0.0106 0.0148 0.0051 0.0063 0.0025 0.0046 0.0008 0.0008 0.0009 0.0004 0.0003
Vacuole Periphery 0.0011 0.0003 0.0002 0.0003 0.0001 0.0003 0.0081 0.0003 0.0022 0.0007 0.001 0.0006 0.0026 0 0.0001 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.5181 11.1922 14.9251 27.2403 15.6447 17.2283 18.9428 26.6218 24.9903 24.7249
Translational Efficiency 0.4354 0.5189 0.5103 0.3434 0.4398 0.4042 0.3566 0.2954 0.3971 0.3253

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Adenylate cyclase; required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation
Localization
Cell Percentages cytoplasm (60%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Cyr1

Cyr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cyr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available