Standard name
Human Ortholog
Description Putative protein of unknown function; functions together with HAM1 to mediate resistance to 5-FU; specifically reduces the incorporation of 5-FU into RNA, without affecting uptake or incorporation of uracil into RNA; proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.99 0.98 0.95 0.91 0.84 0.79 0.66 0.75 0.72 0.69 0.97 0.99 0.99 0.98 0.99 0.94 0.97 0.98 0.97 0.96 0.93 0.97
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.06 0.11 0.15 0.24 0.14 0.23 0.21 0 0 0 0 0 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 2 1 2 3 5 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 0 5 1 0 1 0 0 0 1 0 0 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 1 4 2 4 10 8 14 6 7 5 16 3 2 2 2 2 2 0 0 0 0 0 0
Cytoplasm 94 176 291 259 337 336 337 366 158 222 198 311 210 372 408 227 304 266 114 174 276 265 339 401
Endoplasmic Reticulum 0 1 1 2 8 10 20 24 8 13 6 21 1 2 1 5 9 8 0 0 2 1 1 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2
Golgi 0 0 1 0 0 1 0 2 1 1 0 0 0 0 0 1 0 0 0 0 1 0 1 0
Mitochondria 0 1 0 0 0 2 1 1 18 10 11 18 0 0 0 3 3 1 0 0 0 1 2 0
Nucleus 1 1 0 4 7 8 7 8 1 6 6 10 2 5 3 7 2 0 1 1 0 2 6 3
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 1 2 11 21 45 68 59 41 63 96 9 1 12 3 6 19 1 1 0 0 5 1
Unique Cell Count 96 177 293 264 353 369 401 466 241 296 276 451 217 377 414 232 306 282 118 178 286 277 367 416
Labelled Cell Count 97 180 298 269 367 389 420 485 258 303 292 478 225 382 426 250 326 297 118 178 286 277 367 416


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.8 10.6 14.2 13.6 14.9 13.2 13.4 12.8 11.8 12.5 12.1 12.4 15.8 16.1 16.2 14.7 19.0 19.6 14.8 14.9 15.6
Std Deviation (1e-4) 2.7 1.8 1.9 2.0 2.2 2.0 2.4 2.2 2.3 2.4 2.3 2.5 2.6 2.4 2.2 3.6 4.5 5.3 2.4 3.2 2.8
Intensity Change (Log2) -0.06 0.08 -0.1 -0.08 -0.15 -0.26 -0.18 -0.23 -0.19 0.16 0.18 0.19 0.05 0.42 0.47 0.06 0.07 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1.2 0.6 1.4 0 0.9 0 1.8 0 0 0 0 0 0
Cytoplasm -1.3 -3.0 -4.7 -6.8 -8.2 -10.6 -8.8 -9.5 -10.3 -2.2 -0.8 -0.9 -1.5 0 -3.4
Endoplasmic Reticulum 0 0 0 3.5 3.6 0 3.2 0 3.4 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.8 0 3.5 3.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.6 3.8 5.7 6.6 8.8 6.4 8.5 8.3 0 0 2.5 0 0 4.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.4643 11.3001 10.4873 8.4924 9.3791 10.1918 11.7447 14.7869 13.1625 12.8983 12.8261 13.3056 9.7864 11.9197 10.719 11.6224 8.2472 10.6058
Actin 0.0002 0.0008 0.0001 0.0007 0.0001 0.0002 0.0656 0.001 0.0333 0.0009 0.0002 0.0036 0.0032 0.0001 0.0006 0.0003 0.0003 0.0026
Bud 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0009 0.0001 0.0005 0.0007 0.0001 0.0002 0.0002 0.0001 0.0005 0.0001 0.0002 0.0002
Bud Neck 0.0002 0.0002 0.0002 0.0005 0.0004 0.0006 0.008 0.0001 0.002 0.0011 0.0008 0.0009 0.0003 0.0001 0.0004 0.0003 0.0007 0.0007
Bud Periphery 0 0 0 0 0 0 0.0012 0.0001 0.0004 0.0003 0 0.0001 0.0001 0 0.0004 0 0.0001 0.0001
Bud Site 0.0001 0.0003 0.0001 0 0 0 0.0057 0.0005 0.0054 0.0008 0 0.0002 0.0007 0.0001 0.001 0.0001 0.001 0.0001
Cell Periphery 0 0 0 0 0 0 0.0003 0 0.0001 0 0 0 0 0 0.0001 0.0001 0 0
Cytoplasm 0.9572 0.9708 0.9654 0.9699 0.9598 0.9538 0.7936 0.9765 0.9034 0.9339 0.9885 0.9472 0.9347 0.99 0.9481 0.9545 0.8498 0.945
Cytoplasmic Foci 0.0007 0.0017 0.002 0.0011 0.001 0.0022 0.0197 0.0053 0.0092 0.0104 0.0014 0.0078 0.0041 0.0005 0.0079 0.0024 0.0446 0.0017
Eisosomes 0 0 0 0 0 0 0.0008 0 0.0001 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0015 0.0006 0.0009 0.0011 0.0027 0.0038 0.0063 0.0002 0.0017 0.001 0.0005 0.0012 0.0015 0.0003 0.0018 0.0088 0.0008 0.0025
Endosome 0.0005 0.0013 0.0005 0.0006 0.0012 0.0032 0.0251 0.0007 0.0125 0.0296 0.0004 0.0054 0.0052 0.0001 0.0066 0.0079 0.0635 0.0037
Golgi 0 0.0001 0 0 0 0.0001 0.0146 0.0016 0.0079 0.0033 0 0.0048 0.0008 0 0.0009 0.0003 0.008 0.0009
Lipid Particles 0 0 0 0 0 0 0.0083 0.0035 0.0036 0.0003 0 0.0002 0.0054 0 0.0012 0.0012 0.0037 0
Mitochondria 0.0001 0.0001 0.0001 0.0002 0.0001 0.0002 0.012 0.0002 0.0027 0.0017 0.0001 0.0129 0.0016 0 0.0021 0.0001 0.0007 0.0096
None 0.0012 0.0008 0.0008 0.0016 0.0009 0.001 0.0059 0.0009 0.0013 0.0017 0.0008 0.0015 0.0038 0.0006 0.0029 0.0015 0.0009 0.0008
Nuclear Periphery 0.0048 0.0015 0.0026 0.0023 0.0039 0.005 0.0047 0.0004 0.0018 0.001 0.0006 0.0007 0.0046 0.0006 0.0028 0.0037 0.0014 0.0038
Nucleolus 0 0 0 0 0 0 0.0008 0.0001 0.0001 0 0 0 0.0004 0 0.0007 0 0.0001 0
Nucleus 0.0324 0.0206 0.0263 0.0206 0.0283 0.0281 0.0128 0.0034 0.009 0.009 0.0061 0.0095 0.0264 0.0072 0.0157 0.0156 0.0171 0.027
Peroxisomes 0 0 0.0001 0 0 0 0.0074 0.0018 0.003 0.0004 0 0.0031 0.004 0 0.0006 0 0.0033 0.0001
Punctate Nuclear 0.0003 0.0005 0.0005 0.0009 0.0003 0.0004 0.0041 0.0035 0.0009 0.0004 0.0002 0.0003 0.0017 0.0002 0.0014 0.0006 0.0011 0.0004
Vacuole 0.0006 0.0005 0.0003 0.0003 0.0008 0.0009 0.0015 0.0001 0.0007 0.0027 0.0002 0.0002 0.0009 0.0001 0.0035 0.0022 0.0024 0.0005
Vacuole Periphery 0.0001 0.0001 0.0001 0.0001 0.0002 0.0003 0.0008 0 0.0003 0.0007 0.0001 0.0001 0.0004 0 0.0009 0.0003 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.1355 40.0966 30.7452 46.8791 48.4625 47.8176 51.3527 61.1104 70.7018 56.1983
Translational Efficiency 2.0774 1.7477 2.1536 1.708 1.8703 1.7752 1.7029 1.4408 1.4513 1.7784

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Putative protein of unknown function; functions together with HAM1 to mediate resistance to 5-FU; specifically reduces the incorporation of 5-FU into RNA, without affecting uptake or incorporation of uracil into RNA; proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Log1

Log1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Log1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available