Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 – – – – – –
Cell Periphery 0 0 0 0.06 0 0 0 – 0 0 0 0 0 0 0 0.17 0.13 0.16 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0.07 0.06 0.05 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0.06 0 0 0 0.09 – 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Golgi 0 0 0.17 0.16 0.18 0 0 – 0 0 0 0 0.12 0.15 0.18 0.44 0.34 0.32 0.07 0.09 0.05 0.08 0.07 0.11
Mitochondria 0.87 0.93 0.79 0.87 0.66 0.88 0.68 – 0.88 0.85 0.85 0.87 0.87 0.86 0.78 0.1 0.16 0.11 0.82 0.77 0.8 0.73 0.7 0.65
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.08 0 0.05 0 0.06 0 0.07 – 0.11 0.05 0.08 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0.08 0.09 – 0.08 0.07 0.11 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.08 0.1 0.05 0 0 0.07 0.06 0.06 0.07
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.09 0 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0.13 0.1 0.06 0.15 0.08 0.11 – 0.05 0.08 0 0.06 0.16 0.09 0.08 0.14 0.2 0.26 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 5 7 3 6 0 1 – 0 2 2 0 1 2 2 1 0 1 4 15 2 3 15 14
Bud 1 1 4 7 3 8 6 – 5 10 9 3 1 1 2 2 2 2 0 4 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 – – – – – –
Cell Periphery 3 12 12 11 0 1 3 – 1 3 5 2 9 4 3 13 12 3 0 0 0 0 0 1
Cytoplasm 2 2 3 3 15 9 16 – 7 7 8 7 8 5 22 5 5 1 0 0 0 0 0 1
Endoplasmic Reticulum 3 6 2 5 2 1 1 – 0 0 3 1 0 3 3 3 2 1 0 1 0 0 1 1
Endosome 16 7 17 8 9 4 33 – 5 12 7 11 6 8 12 5 5 0 12 11 12 7 13 12
Golgi 10 16 50 30 51 0 9 – 1 1 5 8 35 42 55 34 32 6 28 43 16 17 24 40
Mitochondria 327 426 227 162 185 240 243 – 268 330 279 320 247 239 235 8 15 2 330 366 254 150 231 231
Nucleus 4 1 0 1 2 4 4 – 4 2 3 1 1 0 0 0 0 0 0 0 0 0 1 0
Nuclear Periphery 31 20 15 4 16 7 24 – 33 19 25 26 15 12 13 2 2 0 6 2 1 1 4 7
Nucleolus 23 11 6 8 12 23 31 – 23 26 37 20 9 11 8 2 2 0 1 1 2 2 3 5
Peroxisomes 12 5 10 2 10 0 14 – 0 12 0 2 7 8 17 6 9 1 12 11 22 11 20 24
SpindlePole 4 1 3 4 8 1 11 – 6 14 11 6 1 1 3 4 8 0 1 2 0 1 0 0
Vac/Vac Membrane 54 58 29 11 42 23 40 – 15 30 12 21 46 24 24 11 19 5 5 14 1 6 8 8
Unique Cell Count 378 460 287 186 281 274 358 306 390 330 368 285 278 301 78 94 19 406 478 318 206 332 354
Labelled Cell Count 490 571 385 259 361 321 436 368 468 406 428 386 360 399 96 113 23 406 478 318 206 332 354


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.4 9.5 9.4 9.0 9.6 6.6 8.6 – 7.8 8.0 7.8 8.2 9.9 9.5 9.5 9.6 9.3 8.4 9.1 9.6 10.0
Std Deviation (1e-4) 1.7 2.0 1.6 1.7 2.1 1.8 1.8 – 1.6 1.6 1.8 1.8 1.7 1.6 1.9 2.7 1.9 1.8 1.9 2.1 2.0
Intensity Change (Log2) – – – -0.07 0.02 -0.51 -0.13 – -0.28 -0.23 -0.27 -0.2 0.07 0.01 0.01 0.02 -0.02 -0.18 -0.05 0.03 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -0.2 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1.2 0 – 0 1.1 1.2 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.9 -3.5 -3.0 -2.8 – -3.2 -3.0 -2.0 -3.2 -0.7 -2.0 -2.4 3.9 3.0 0
Cytoplasm 0 2.9 1.8 2.6 – 0 0 1.3 0 1.5 0 3.8 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome -0.8 -1.6 -2.8 1.6 – -2.8 -1.8 -2.4 -1.8 -2.3 -1.8 -1.1 0 -0.2 0
Golgi -0.4 0.2 -7.2 -6.5 – -7.4 -8.4 -6.9 -6.8 -1.7 -0.7 0.3 4.9 3.4 0
Mitochondria 2.2 -3.5 2.7 -3.2 – 2.8 1.9 1.8 2.7 2.4 2.1 -0.3 -11.3 -11.0 -6.7
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.7 0.2 -1.6 0.8 – 2.5 -0.2 1.2 1.0 0 -0.5 -0.5 0 0 0
Nucleolus 1.4 1.5 3.4 3.6 – 3.1 2.8 4.4 2.2 0.8 1.3 0.5 0 0 0
Peroxisomes 0 0 0 0.3 – 0 -0.3 0 -2.8 -0.7 -0.4 1.3 0 0 0
SpindlePole 0 1.6 0 1.8 – 0 2.1 1.9 0 0 0 0 0 0 0
Vacuole -1.6 1.7 -0.7 0.4 – -2.4 -1.1 -3.2 -2.1 2.1 -0.6 -0.9 1.0 2.6 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.2857 4.5638 4.8483 5.0742 4.6001 4.9708 6.3255 6.5551 6.4263 5.7931 6.2375 6.641 11.4949 10.6252 10.5038 9.9353 10.7285 10.7867
Actin 0.002 0.0072 0.001 0.0002 0.0003 0.0007 0.0388 0.0061 0.0043 0.002 0.0149 0.0069 0.0033 0.0031 0.0022 0.0041 0.0012 0.0048
Bud 0.0002 0.0004 0.0002 0 0 0 0.0004 0.0008 0.0002 0.0005 0.0002 0.0001 0.0001 0.0002 0.0015 0.0002 0.0002 0.0001
Bud Neck 0.0003 0.0003 0 0 0 0 0.0011 0.0002 0.0002 0.003 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003
Bud Periphery 0.0004 0.0007 0.0003 0.0002 0 0.0001 0.0008 0.001 0.0003 0.0005 0.0003 0.0001 0.0003 0.0005 0.0041 0.0006 0.0005 0.0002
Bud Site 0.0009 0.0025 0.0007 0 0 0.0001 0.0012 0.0024 0.0005 0.0049 0.0002 0.0002 0.0002 0.0006 0.0007 0.0002 0.0002 0.0001
Cell Periphery 0.0002 0.0005 0.0001 0.0002 0 0.0001 0.0003 0.0002 0.0001 0.0003 0.0001 0.0001 0.0002 0.0004 0.0001 0.0003 0.0002 0.0001
Cytoplasm 0.0003 0.0001 0 0 0 0 0.0014 0.0004 0.0027 0.0026 0.0002 0.0001 0 0.0001 0.0001 0.0001 0 0
Cytoplasmic Foci 0.0043 0.0039 0.001 0.0001 0.0002 0.0005 0.018 0.0033 0.0063 0.0127 0.0024 0.003 0.0021 0.0026 0.0042 0.0012 0.0026 0.0006
Eisosomes 0.0002 0.0002 0.0001 0.0001 0 0.0001 0.0006 0.0002 0.0001 0.0001 0.0003 0.0002 0.0002 0.0002 0.0001 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0002 0.0004 0 0 0 0 0.0031 0.0003 0.0003 0.0005 0.0007 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001
Endosome 0.0169 0.0177 0.0048 0.0007 0.002 0.0034 0.0253 0.0186 0.0114 0.0374 0.0206 0.0077 0.0048 0.0085 0.0104 0.0061 0.0029 0.0037
Golgi 0.0107 0.0298 0.0163 0.0016 0.0037 0.0101 0.0393 0.0197 0.0394 0.0384 0.0118 0.0199 0.0136 0.018 0.018 0.0083 0.0067 0.0218
Lipid Particles 0.038 0.0211 0.0008 0.0011 0.0002 0.0035 0.0683 0.0102 0.0138 0.0184 0.0076 0.0129 0.0259 0.0164 0.0155 0.0026 0.0051 0.0078
Mitochondria 0.8889 0.8648 0.9629 0.9893 0.9924 0.9758 0.7273 0.8984 0.896 0.8662 0.9244 0.937 0.9287 0.9189 0.9166 0.9494 0.9528 0.9506
None 0.0007 0.0001 0 0 0 0 0.004 0.0001 0.0006 0.0002 0.0013 0.0001 0 0 0 0 0 0
Nuclear Periphery 0.0008 0.0006 0 0 0 0.0008 0.0194 0.003 0.0022 0.0002 0.0019 0.0001 0.0002 0.0001 0 0.0001 0 0.0001
Nucleolus 0.0014 0.0098 0.0002 0 0 0 0.0017 0.0004 0.0007 0.0002 0.0001 0.0001 0.0001 0.0009 0.0003 0.0001 0.0001 0.0001
Nucleus 0.0006 0.0006 0.0001 0 0 0 0.0019 0.0031 0.0029 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0 0
Peroxisomes 0.0065 0.008 0.0023 0.0011 0.0006 0.0015 0.0343 0.0146 0.0137 0.0053 0.007 0.007 0.0134 0.0165 0.0178 0.0075 0.0239 0.0058
Punctate Nuclear 0.0016 0 0 0 0 0 0.0027 0 0.0001 0.0003 0.0008 0.0001 0 0 0 0 0 0
Vacuole 0.0072 0.0102 0.005 0.0017 0.0001 0.0005 0.004 0.0051 0.0015 0.0039 0.0024 0.001 0.0019 0.0034 0.0014 0.0052 0.0007 0.0008
Vacuole Periphery 0.0177 0.021 0.004 0.0035 0.0004 0.0028 0.0062 0.0117 0.0025 0.0023 0.0028 0.0028 0.0047 0.0093 0.0065 0.0136 0.0026 0.003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.003 20.6355 21.4374 24.61 27.9338 19.9949 31.6445 36.1442 33.8135 32.563
Translational Efficiency 3.5801 1.5858 1.6382 1.7394 1.5571 2.417 1.5761 1.6326 2.0972 1.903

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mrpl8

Mrpl8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrpl8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available