Standard name | |
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Human Ortholog | |
Description | Nuclear actin-related protein involved in chromatin remodeling; component of chromatin-remodeling enzyme complexes |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | 1 | 1 | 1 | 0 | 2 | 1 |
Bud | 0 | 0 | 0 | – | 0 | 1 | 1 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | – | – | – | 0 | 0 | 2 | 0 | 1 | 6 |
Bud Neck | 0 | 0 | 0 | – | 0 | 1 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 2 | 0 | 1 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 42 | 74 | 66 | – | 44 | 36 | 69 | 124 | 47 | 72 | 11 | 28 | 110 | 121 | 160 | – | – | – | 46 | 76 | 43 | 81 | 75 | 75 |
Endoplasmic Reticulum | 0 | 0 | 1 | – | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 3 | 3 | 1 | 0 | 1 | 1 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | 3 | 0 | 0 | 0 | 1 | 2 |
Mitochondria | 9 | 8 | 1 | – | 4 | 7 | 20 | 22 | 17 | 28 | 9 | 30 | 7 | 5 | 4 | – | – | – | 8 | 3 | 12 | 8 | 5 | 9 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 3 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | 0 | 0 | 0 | 0 | 2 | 0 |
Nucleolus | 1 | 0 | 0 | – | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | – | – | – | 0 | 1 | 2 | 0 | 2 | 1 |
Vac/Vac Membrane | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | – | – | – | 4 | 3 | 1 | 1 | 3 | 4 |
Unique Cell Count | 49 | 79 | 71 | 45 | 39 | 80 | 135 | 58 | 87 | 17 | 53 | 113 | 124 | 164 | 76 | 95 | 74 | 96 | 103 | 112 | ||||
Labelled Cell Count | 52 | 82 | 71 | 49 | 46 | 93 | 160 | 66 | 103 | 23 | 61 | 120 | 130 | 166 | 76 | 95 | 74 | 96 | 103 | 112 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.2 | 3.3 | 4.8 | – | 3.6 | 3.8 | 2.5 | 3.2 | 2.5 | 2.4 | 3.0 | 2.6 | 3.4 | 3.4 | 3.6 | – | – | – | 3.7 | 4.1 | 4.5 |
Std Deviation (1e-4) | 1.5 | 0.7 | 3.4 | – | 2.5 | 4.7 | 1.3 | 2.0 | 1.4 | 1.4 | 2.3 | 2.6 | 1.1 | 1.5 | 1.4 | – | – | – | 1.6 | 2.1 | 2.5 |
Intensity Change (Log2) | – | – | – | – | -0.41 | -0.34 | -0.97 | -0.61 | -0.96 | -1.02 | -0.69 | -0.9 | -0.53 | -0.53 | -0.44 | – | – | – | -0.37 | -0.25 | -0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.6644 | 1.1182 | 0.7833 | 0.2632 | 0.5928 | 0.4347 | 0.7695 | 0.5263 | 0.1171 | 0.4393 | 0.3474 | 0.7861 | 3.0481 | 0.7921 | 1.7614 | 0.5047 | 1.278 | 0.5249 |
Actin | 0.0546 | 0.0021 | 0.0311 | 0.0041 | 0 | 0.0007 | 0.0072 | 0.001 | 0.0074 | 0.0037 | 0.039 | 0.023 | 0.1986 | 0.0001 | 0.0231 | 0.0001 | 0.0006 | 0.0015 |
Bud | 0.0014 | 0.0038 | 0.0094 | 0.001 | 0.0003 | 0.0009 | 0.0008 | 0.0002 | 0.0001 | 0 | 0.0015 | 0.0002 | 0.0019 | 0 | 0.0012 | 0 | 0 | 0.0001 |
Bud Neck | 0.0024 | 0.0001 | 0.0012 | 0.0223 | 0.0001 | 0.0006 | 0.001 | 0.0044 | 0.0001 | 0.0019 | 0.002 | 0.0005 | 0.0016 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 |
Bud Periphery | 0.0048 | 0.0078 | 0.0054 | 0.0026 | 0.0002 | 0.003 | 0.0011 | 0.0001 | 0.0001 | 0.0001 | 0.0032 | 0.0007 | 0.004 | 0 | 0.0019 | 0 | 0 | 0.0002 |
Bud Site | 0.0145 | 0.0092 | 0.0117 | 0.0042 | 0.0008 | 0.0003 | 0.0082 | 0.0164 | 0.0006 | 0.0007 | 0.0698 | 0.0002 | 0.0041 | 0 | 0.0033 | 0 | 0.0004 | 0.0001 |
Cell Periphery | 0.0061 | 0.0009 | 0.0006 | 0.0014 | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0.0001 | 0.0003 | 0.0026 | 0.0001 | 0.0006 | 0.0001 | 0.0005 | 0 | 0 | 0.0001 |
Cytoplasm | 0.0307 | 0.0159 | 0.0088 | 0.0121 | 0.0035 | 0.0289 | 0.0296 | 0.0144 | 0.0182 | 0.009 | 0.0126 | 0.0345 | 0.0041 | 0.0589 | 0.0338 | 0.0143 | 0.0055 | 0.0918 |
Cytoplasmic Foci | 0.0083 | 0.003 | 0.0017 | 0.004 | 0.0075 | 0.0021 | 0.0143 | 0.004 | 0.005 | 0.0129 | 0.0062 | 0.0131 | 0.0154 | 0.0012 | 0.0198 | 0.0028 | 0.0925 | 0.0362 |
Eisosomes | 0.0022 | 0.0004 | 0.001 | 0.0003 | 0 | 0.0001 | 0.0011 | 0.0003 | 0.0004 | 0.0017 | 0.0008 | 0.0005 | 0.0022 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0067 | 0.0006 | 0.007 | 0.0002 | 0.0003 | 0.0002 | 0.004 | 0.0003 | 0.0005 | 0.0006 | 0.0003 | 0.0007 | 0.01 | 0.0009 | 0.0051 | 0.0003 | 0.0003 | 0.0031 |
Endosome | 0.0269 | 0.0056 | 0.0035 | 0.0073 | 0.1689 | 0.0002 | 0.046 | 0.0069 | 0.0013 | 0.0011 | 0.0006 | 0.0015 | 0.0536 | 0.0004 | 0.0414 | 0.0003 | 0.024 | 0.0014 |
Golgi | 0.008 | 0.0069 | 0.0035 | 0.0066 | 0.0133 | 0.0001 | 0.0152 | 0.0109 | 0.0008 | 0.0006 | 0.0013 | 0.0032 | 0.0359 | 0 | 0.0058 | 0 | 0.0083 | 0.0004 |
Lipid Particles | 0.0167 | 0.0012 | 0.0009 | 0.0134 | 0.0026 | 0.0003 | 0.0081 | 0.0003 | 0.001 | 0.0041 | 0.0034 | 0.0075 | 0.0587 | 0.0001 | 0.0415 | 0.0004 | 0.0592 | 0.0034 |
Mitochondria | 0.0256 | 0.0054 | 0.0024 | 0.0164 | 0.0047 | 0.0007 | 0.0065 | 0.0007 | 0.0003 | 0.0006 | 0.0054 | 0.0216 | 0.023 | 0.0001 | 0.0098 | 0.0001 | 0.0003 | 0.0006 |
None | 0.7073 | 0.9345 | 0.8843 | 0.887 | 0.7472 | 0.9591 | 0.8208 | 0.9378 | 0.9614 | 0.953 | 0.8355 | 0.8782 | 0.5114 | 0.937 | 0.7447 | 0.9806 | 0.7949 | 0.8495 |
Nuclear Periphery | 0.0238 | 0.0003 | 0.0146 | 0.0001 | 0.0003 | 0.0001 | 0.0011 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0359 | 0.0002 | 0.0196 | 0.0001 | 0.0001 | 0.0025 |
Nucleolus | 0.0024 | 0.0002 | 0.0003 | 0.0017 | 0.0004 | 0.0001 | 0.0166 | 0.0002 | 0.0001 | 0.0013 | 0.0011 | 0.0002 | 0.0019 | 0 | 0.0029 | 0.0001 | 0.0001 | 0.0002 |
Nucleus | 0.0148 | 0.0003 | 0.0105 | 0.0006 | 0.0004 | 0.0001 | 0.0033 | 0.0006 | 0.0001 | 0.0006 | 0.0006 | 0.0004 | 0.003 | 0.0003 | 0.0069 | 0.0001 | 0 | 0.0022 |
Peroxisomes | 0.0057 | 0.0004 | 0.0002 | 0.0067 | 0.0009 | 0.0018 | 0.0025 | 0.0004 | 0.0016 | 0.003 | 0.0123 | 0.0104 | 0.0136 | 0 | 0.0121 | 0.0001 | 0.0112 | 0.0032 |
Punctate Nuclear | 0.0085 | 0.0003 | 0.0006 | 0.0002 | 0.0001 | 0.0002 | 0.003 | 0.0007 | 0.0006 | 0.0043 | 0.0009 | 0.0028 | 0.0114 | 0.0002 | 0.0095 | 0.0004 | 0.0022 | 0.0025 |
Vacuole | 0.0229 | 0.0007 | 0.0008 | 0.0066 | 0.0358 | 0.0001 | 0.0069 | 0.0001 | 0.0002 | 0.0003 | 0.0006 | 0.0003 | 0.0032 | 0.0002 | 0.0131 | 0.0001 | 0.0002 | 0.0005 |
Vacuole Periphery | 0.0055 | 0.0003 | 0.0007 | 0.0012 | 0.0126 | 0 | 0.0018 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.006 | 0 | 0.0029 | 0 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 40.1579 | 36.2342 | 34.3561 | 33.4821 | 38.4416 | 32.2586 | 38.5021 | 38.4644 | 35.8537 | 37.5919 |
Translational Efficiency | 0.8548 | 0.8386 | 0.7403 | 0.7695 | 0.6714 | 0.8949 | 0.6424 | 0.6431 | 0.6781 | 0.7035 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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