Standard name
Human Ortholog
Description Nuclear actin-related protein involved in chromatin remodeling; component of chromatin-remodeling enzyme complexes

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.12 0 0 0 0 0 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.86 0.94 0.93 0.98 0.92 0.86 0.92 0.81 0.83 0.65 0.53 0.97 0.98 0.98 0.62 0.8 0.58 0.85 0.73 0.68
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Mitochondria 0.18 0.1 0 0.09 0.18 0.25 0.16 0.29 0.32 0.53 0.57 0.06 0 0 0.11 0 0.16 0.09 0.06 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.06 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 1 0 2 1
Bud 0 0 0 0 1 1 1 0 0 2 2 0 0 0 0 0 2 0 1 6
Bud Neck 0 0 0 0 1 0 4 0 0 0 0 0 0 0 2 0 1 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 2 0 0 0 0 1 0 0 0 0 1 0 0 0
Cytoplasm 42 74 66 44 36 69 124 47 72 11 28 110 121 160 46 76 43 81 75 75
Endoplasmic Reticulum 0 0 1 0 0 0 1 0 1 0 0 0 1 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 1 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 0 1 2
Mitochondria 9 8 1 4 7 20 22 17 28 9 30 7 5 4 8 3 12 8 5 9
Nucleus 0 0 0 0 0 3 2 1 0 0 0 1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 0
Nucleolus 1 0 0 0 1 0 3 0 0 0 0 1 0 0 0 0 0 0 1 0
Peroxisomes 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 1 1 1 0 0 0 1 0 0 1 2 0 2 1
Vac/Vac Membrane 0 0 1 0 0 0 0 0 1 1 0 0 1 1 4 3 1 1 3 4
Unique Cell Count 49 79 71 45 39 80 135 58 87 17 53 113 124 164 76 95 74 96 103 112
Labelled Cell Count 52 82 71 49 46 93 160 66 103 23 61 120 130 166 76 95 74 96 103 112


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 3.3 4.8 3.6 3.8 2.5 3.2 2.5 2.4 3.0 2.6 3.4 3.4 3.6 3.7 4.1 4.5
Std Deviation (1e-4) 1.5 0.7 3.4 2.5 4.7 1.3 2.0 1.4 1.4 2.3 2.6 1.1 1.5 1.4 1.6 2.1 2.5
Intensity Change (Log2) -0.41 -0.34 -0.97 -0.61 -0.96 -1.02 -0.69 -0.9 -0.53 -0.53 -0.44 -0.37 -0.25 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6644 1.1182 0.7833 0.2632 0.5928 0.4347 0.7695 0.5263 0.1171 0.4393 0.3474 0.7861 3.0481 0.7921 1.7614 0.5047 1.278 0.5249
Actin 0.0546 0.0021 0.0311 0.0041 0 0.0007 0.0072 0.001 0.0074 0.0037 0.039 0.023 0.1986 0.0001 0.0231 0.0001 0.0006 0.0015
Bud 0.0014 0.0038 0.0094 0.001 0.0003 0.0009 0.0008 0.0002 0.0001 0 0.0015 0.0002 0.0019 0 0.0012 0 0 0.0001
Bud Neck 0.0024 0.0001 0.0012 0.0223 0.0001 0.0006 0.001 0.0044 0.0001 0.0019 0.002 0.0005 0.0016 0 0.0004 0 0.0001 0.0001
Bud Periphery 0.0048 0.0078 0.0054 0.0026 0.0002 0.003 0.0011 0.0001 0.0001 0.0001 0.0032 0.0007 0.004 0 0.0019 0 0 0.0002
Bud Site 0.0145 0.0092 0.0117 0.0042 0.0008 0.0003 0.0082 0.0164 0.0006 0.0007 0.0698 0.0002 0.0041 0 0.0033 0 0.0004 0.0001
Cell Periphery 0.0061 0.0009 0.0006 0.0014 0.0001 0.0001 0.0007 0.0002 0.0001 0.0003 0.0026 0.0001 0.0006 0.0001 0.0005 0 0 0.0001
Cytoplasm 0.0307 0.0159 0.0088 0.0121 0.0035 0.0289 0.0296 0.0144 0.0182 0.009 0.0126 0.0345 0.0041 0.0589 0.0338 0.0143 0.0055 0.0918
Cytoplasmic Foci 0.0083 0.003 0.0017 0.004 0.0075 0.0021 0.0143 0.004 0.005 0.0129 0.0062 0.0131 0.0154 0.0012 0.0198 0.0028 0.0925 0.0362
Eisosomes 0.0022 0.0004 0.001 0.0003 0 0.0001 0.0011 0.0003 0.0004 0.0017 0.0008 0.0005 0.0022 0.0002 0.0006 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.0067 0.0006 0.007 0.0002 0.0003 0.0002 0.004 0.0003 0.0005 0.0006 0.0003 0.0007 0.01 0.0009 0.0051 0.0003 0.0003 0.0031
Endosome 0.0269 0.0056 0.0035 0.0073 0.1689 0.0002 0.046 0.0069 0.0013 0.0011 0.0006 0.0015 0.0536 0.0004 0.0414 0.0003 0.024 0.0014
Golgi 0.008 0.0069 0.0035 0.0066 0.0133 0.0001 0.0152 0.0109 0.0008 0.0006 0.0013 0.0032 0.0359 0 0.0058 0 0.0083 0.0004
Lipid Particles 0.0167 0.0012 0.0009 0.0134 0.0026 0.0003 0.0081 0.0003 0.001 0.0041 0.0034 0.0075 0.0587 0.0001 0.0415 0.0004 0.0592 0.0034
Mitochondria 0.0256 0.0054 0.0024 0.0164 0.0047 0.0007 0.0065 0.0007 0.0003 0.0006 0.0054 0.0216 0.023 0.0001 0.0098 0.0001 0.0003 0.0006
None 0.7073 0.9345 0.8843 0.887 0.7472 0.9591 0.8208 0.9378 0.9614 0.953 0.8355 0.8782 0.5114 0.937 0.7447 0.9806 0.7949 0.8495
Nuclear Periphery 0.0238 0.0003 0.0146 0.0001 0.0003 0.0001 0.0011 0.0001 0.0001 0.0003 0.0003 0.0003 0.0359 0.0002 0.0196 0.0001 0.0001 0.0025
Nucleolus 0.0024 0.0002 0.0003 0.0017 0.0004 0.0001 0.0166 0.0002 0.0001 0.0013 0.0011 0.0002 0.0019 0 0.0029 0.0001 0.0001 0.0002
Nucleus 0.0148 0.0003 0.0105 0.0006 0.0004 0.0001 0.0033 0.0006 0.0001 0.0006 0.0006 0.0004 0.003 0.0003 0.0069 0.0001 0 0.0022
Peroxisomes 0.0057 0.0004 0.0002 0.0067 0.0009 0.0018 0.0025 0.0004 0.0016 0.003 0.0123 0.0104 0.0136 0 0.0121 0.0001 0.0112 0.0032
Punctate Nuclear 0.0085 0.0003 0.0006 0.0002 0.0001 0.0002 0.003 0.0007 0.0006 0.0043 0.0009 0.0028 0.0114 0.0002 0.0095 0.0004 0.0022 0.0025
Vacuole 0.0229 0.0007 0.0008 0.0066 0.0358 0.0001 0.0069 0.0001 0.0002 0.0003 0.0006 0.0003 0.0032 0.0002 0.0131 0.0001 0.0002 0.0005
Vacuole Periphery 0.0055 0.0003 0.0007 0.0012 0.0126 0 0.0018 0.0001 0 0.0001 0.0001 0.0002 0.006 0 0.0029 0 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 40.1579 36.2342 34.3561 33.4821 38.4416 32.2586 38.5021 38.4644 35.8537 37.5919
Translational Efficiency 0.8548 0.8386 0.7403 0.7695 0.6714 0.8949 0.6424 0.6431 0.6781 0.7035

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Nuclear actin-related protein involved in chromatin remodeling; component of chromatin-remodeling enzyme complexes
Localization
Cell Percentages nucleus (31%), cytoplasm (4%), mixed (48%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Arp4

Arp4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arp4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available