Standard name | |
---|---|
Human Ortholog | |
Description | Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 |
Bud | 2 | 1 | 1 | 0 | 2 | 4 | 7 | 3 | 2 | 3 | 8 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 3 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 7 | 0 | 0 | 4 | 15 | 12 | 16 | 20 | 11 | 23 | 2 | 2 | 9 | 10 | 8 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 304 | 135 | 274 | 144 | 186 | 253 | 346 | 269 | 237 | 267 | 239 | 237 | 473 | 535 | 726 | 169 | 227 | 181 | 298 | 129 | 260 | 453 | 481 | 496 |
Endoplasmic Reticulum | 3 | 0 | 1 | 0 | 0 | 3 | 4 | 3 | 11 | 15 | 3 | 7 | 5 | 0 | 0 | 7 | 4 | 7 | 1 | 0 | 1 | 0 | 3 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 2 | 7 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 3 | 2 |
Mitochondria | 7 | 1 | 6 | 8 | 10 | 91 | 133 | 95 | 133 | 172 | 185 | 162 | 1 | 0 | 0 | 1 | 2 | 2 | 1 | 0 | 3 | 2 | 6 | 5 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 7 | 6 | 3 | 7 | 8 | 2 | 0 | 1 | 5 | 2 | 1 | 0 | 1 | 0 | 2 | 3 | 1 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 4 | 3 |
Vac/Vac Membrane | 0 | 0 | 1 | 1 | 0 | 14 | 29 | 39 | 56 | 72 | 73 | 31 | 1 | 2 | 3 | 4 | 6 | 5 | 0 | 7 | 0 | 3 | 5 | 1 |
Unique Cell Count | 312 | 135 | 279 | 148 | 191 | 315 | 469 | 374 | 382 | 446 | 424 | 409 | 483 | 539 | 730 | 177 | 237 | 187 | 308 | 144 | 283 | 479 | 527 | 539 |
Labelled Cell Count | 317 | 138 | 290 | 153 | 199 | 371 | 539 | 429 | 462 | 555 | 529 | 475 | 485 | 541 | 740 | 196 | 250 | 205 | 308 | 144 | 283 | 479 | 527 | 539 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.6 | 5.9 | 5.5 | 5.7 | 6.6 | 6.5 | 6.6 | 6.9 | 6.7 | 6.8 | 6.4 | 6.6 | 6.6 | 7.5 | 7.6 | 10.0 | 9.5 | 10.0 | 6.9 | 7.7 | 8.1 |
Std Deviation (1e-4) | 0.9 | 1.0 | 1.0 | 1.3 | 1.1 | 1.2 | 1.3 | 1.5 | 1.4 | 1.5 | 1.6 | 1.5 | 1.4 | 1.3 | 1.2 | 1.6 | 2.1 | 2.0 | 1.2 | 1.4 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.27 | 0.25 | 0.26 | 0.32 | 0.29 | 0.31 | 0.22 | 0.27 | 0.27 | 0.44 | 0.46 | 0.86 | 0.79 | 0.86 | 0.33 | 0.48 | 0.55 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | -1.1 | 0.5 | 0.5 | 1.2 | 1.4 | 0.1 | 2.0 | 0 | 0 | 0 | 1.7 | 0.6 | 0.8 |
Cytoplasm | -0.6 | -0.6 | -6.9 | -8.6 | -8.9 | -11.0 | -11.5 | -12.2 | -11.9 | -0.3 | 1.4 | 1.9 | -1.7 | -1.6 | -1.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 2.4 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1.8 | 8.8 | 8.9 | 8.1 | 10.2 | 11.1 | 12.1 | 11.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 3.2 | 3.9 | 5.3 | 6.5 | 6.9 | 7.1 | 4.4 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.8826 | 3.9127 | 3.3032 | 3.0434 | 2.8504 | 3.2709 | 2.3522 | 3.0284 | 2.5887 | 2.3707 | 2.6165 | 2.7454 | 1.7933 | 2.5097 | 1.7775 | 1.9095 | 1.6351 | 1.9852 |
Actin | 0.0416 | 0.0016 | 0.0109 | 0.0032 | 0.0289 | 0.0041 | 0.0186 | 0.0004 | 0.0142 | 0.0107 | 0.0104 | 0.0046 | 0.0464 | 0.0073 | 0.0157 | 0.0002 | 0.0062 | 0.0056 |
Bud | 0.0027 | 0.0021 | 0.001 | 0.0025 | 0.0005 | 0.0004 | 0.0015 | 0.0012 | 0.0007 | 0.0055 | 0.0015 | 0.0009 | 0.0005 | 0.0137 | 0.0097 | 0.0003 | 0.0005 | 0.0007 |
Bud Neck | 0.0009 | 0.0004 | 0.0003 | 0.0046 | 0.0006 | 0.0007 | 0.0065 | 0.0002 | 0.0028 | 0.003 | 0.0016 | 0.0027 | 0.0041 | 0.0001 | 0.0008 | 0.0001 | 0.0007 | 0.0052 |
Bud Periphery | 0.0037 | 0.0008 | 0.0007 | 0.0031 | 0.0005 | 0.0003 | 0.0017 | 0.0008 | 0.0006 | 0.007 | 0.0022 | 0.0011 | 0.0008 | 0.0076 | 0.0032 | 0.0002 | 0.0003 | 0.0008 |
Bud Site | 0.0042 | 0.0079 | 0.0016 | 0.0014 | 0.0013 | 0.0001 | 0.0118 | 0.0093 | 0.0049 | 0.0024 | 0.0046 | 0.0003 | 0.0017 | 0.0049 | 0.0055 | 0.0001 | 0.0033 | 0.0004 |
Cell Periphery | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.6031 | 0.8249 | 0.7325 | 0.6501 | 0.717 | 0.7836 | 0.6422 | 0.8665 | 0.7674 | 0.811 | 0.7272 | 0.8496 | 0.589 | 0.8343 | 0.7793 | 0.7889 | 0.7418 | 0.8765 |
Cytoplasmic Foci | 0.0356 | 0.0057 | 0.0096 | 0.0152 | 0.0218 | 0.0152 | 0.045 | 0.0069 | 0.0161 | 0.0132 | 0.0318 | 0.0181 | 0.0483 | 0.0076 | 0.0089 | 0.0074 | 0.0254 | 0.0145 |
Eisosomes | 0.0007 | 0 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0018 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0035 | 0.0032 | 0.0039 | 0.003 | 0.0017 | 0.0025 | 0.0068 | 0.0015 | 0.0027 | 0.0029 | 0.0022 | 0.0015 | 0.0089 | 0.0026 | 0.0025 | 0.003 | 0.0028 | 0.0023 |
Endosome | 0.0097 | 0.0057 | 0.0038 | 0.0132 | 0.004 | 0.0072 | 0.0373 | 0.0033 | 0.0133 | 0.0082 | 0.0221 | 0.0064 | 0.0345 | 0.0016 | 0.009 | 0.002 | 0.0333 | 0.0036 |
Golgi | 0.007 | 0.0003 | 0.0006 | 0.0031 | 0.0038 | 0.0011 | 0.0096 | 0.0002 | 0.0034 | 0.003 | 0.0157 | 0.0011 | 0.0105 | 0.0014 | 0.0013 | 0.0002 | 0.0087 | 0.0002 |
Lipid Particles | 0.0086 | 0.0002 | 0.0009 | 0.0044 | 0.0049 | 0.0006 | 0.0103 | 0.0001 | 0.0038 | 0.0017 | 0.0177 | 0.0018 | 0.0209 | 0.0117 | 0.0011 | 0 | 0.004 | 0.0002 |
Mitochondria | 0.0043 | 0.0003 | 0.0004 | 0.0023 | 0.0016 | 0.0005 | 0.006 | 0.0003 | 0.0023 | 0.0009 | 0.0475 | 0.0009 | 0.0029 | 0.0007 | 0.0009 | 0.0002 | 0.0021 | 0.0002 |
None | 0.2345 | 0.1356 | 0.2244 | 0.2671 | 0.1822 | 0.1737 | 0.1238 | 0.1023 | 0.1439 | 0.1157 | 0.0718 | 0.0945 | 0.1774 | 0.0972 | 0.1502 | 0.1941 | 0.0805 | 0.0805 |
Nuclear Periphery | 0.0029 | 0.0015 | 0.0013 | 0.0019 | 0.0005 | 0.0017 | 0.012 | 0.0009 | 0.0037 | 0.0014 | 0.0023 | 0.0016 | 0.0167 | 0.0008 | 0.0009 | 0.0006 | 0.0212 | 0.0011 |
Nucleolus | 0.0006 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0017 | 0.0001 | 0.0006 | 0.0002 | 0.0023 | 0.0001 | 0.0014 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0001 |
Nucleus | 0.0025 | 0.0043 | 0.0018 | 0.0045 | 0.0013 | 0.0023 | 0.0279 | 0.0033 | 0.0074 | 0.0055 | 0.006 | 0.0048 | 0.0055 | 0.002 | 0.0049 | 0.0014 | 0.0111 | 0.0032 |
Peroxisomes | 0.0249 | 0.0001 | 0.0009 | 0.0122 | 0.0269 | 0.0013 | 0.0185 | 0.0002 | 0.0045 | 0.0025 | 0.0174 | 0.005 | 0.0115 | 0.0048 | 0.0013 | 0.0001 | 0.0502 | 0.0004 |
Punctate Nuclear | 0.0074 | 0.0031 | 0.0034 | 0.0014 | 0.0016 | 0.0039 | 0.0132 | 0.0008 | 0.0048 | 0.0027 | 0.012 | 0.0039 | 0.0134 | 0.0007 | 0.001 | 0.0005 | 0.0052 | 0.0037 |
Vacuole | 0.001 | 0.0016 | 0.001 | 0.0052 | 0.0005 | 0.0007 | 0.0041 | 0.0014 | 0.0021 | 0.0019 | 0.0028 | 0.001 | 0.0023 | 0.0007 | 0.0028 | 0.0005 | 0.0017 | 0.0006 |
Vacuole Periphery | 0.0003 | 0.0002 | 0.0002 | 0.0008 | 0.0001 | 0.0002 | 0.0012 | 0.0001 | 0.0006 | 0.0004 | 0.0009 | 0.0002 | 0.0014 | 0.0001 | 0.0004 | 0.0001 | 0.0007 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.6835 | 12.3388 | 9.6945 | 13.75 | 7.8952 | 12.9836 | 12.4757 | 11.8874 | 14.2201 | 11.5253 |
Translational Efficiency | 1.1898 | 0.7774 | 1.0652 | 1.0628 | 0.9862 | 0.7904 | 0.8611 | 1.1526 | 0.9813 | 0.8789 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|