Standard name
Human Ortholog
Description Protein of unknown function; contains a J-domain, which is a region with homology to the E. coli DnaJ protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.79 0.6 0.87 0.77 0.76 0.59 0.54 0.52 0.41 0.43 0.4 0.38 0.92 0.95 0.94 – 0.86 0.82 0.66 0.77 0.63 0.76 0.83 0.77
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.31 0.45 0.18 0.23 0.22 0.53 0.56 0.56 0.72 0.71 0.75 0.8 0 0 0 – 0.05 0 0.12 0 0.15 0.05 0.07 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0.08 0.14 0.12 0.05 0.07 0 0 0 0 0 0.09 0 0 – 0.1 0.07 0.08 0.11 0.08 0.08 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 1 6 0 3 1 1 5
Bud 0 0 3 0 1 7 6 9 13 13 5 7 3 0 1 – 0 1 1 0 1 0 3 8
Bud Neck 0 0 0 0 1 2 0 3 0 1 1 0 0 0 0 – 0 2 1 0 1 0 0 2
Bud Site 0 0 0 0 0 0 1 3 5 3 5 5 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 0 0 1 1 0 0 0 2 – 0 0 0 0 0 0 0 0
Cytoplasm 261 12 173 151 221 249 306 293 164 214 157 185 183 501 426 – 213 190 250 54 125 127 279 309
Endoplasmic Reticulum 2 1 1 0 1 0 0 0 1 0 0 0 0 1 4 – 16 17 1 0 3 1 1 2
Endosome 0 0 1 0 5 3 3 3 5 1 0 0 2 10 11 – 11 15 11 0 4 2 1 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 – 1 0 0 0 1 0 1 2
Mitochondria 102 9 35 44 63 224 320 315 287 354 294 392 5 2 1 – 13 6 44 2 30 9 22 23
Nucleus 0 0 1 0 0 5 8 15 16 13 13 11 0 0 0 – 3 2 6 1 1 4 4 1
Nuclear Periphery 1 2 0 0 0 0 3 1 0 3 2 3 0 0 0 – 1 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 2 2 2 0 1 1 0 0 1 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 0 1 1 0 0 0 0 2 – 0 0 0 0 0 0 0 0
SpindlePole 1 0 2 0 0 4 10 14 7 11 5 2 0 4 7 – 6 7 9 0 5 3 4 9
Vac/Vac Membrane 12 1 16 27 34 22 42 11 17 21 11 17 18 23 8 – 26 16 30 7 15 13 10 20
Unique Cell Count 330 20 198 195 289 419 571 567 398 496 393 487 200 529 451 249 232 378 71 199 169 337 400
Labelled Cell Count 379 25 232 222 326 517 703 669 518 636 495 623 211 542 463 290 257 378 71 199 169 337 400


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 3.3 3.7 3.7 3.6 3.2 2.8 3.2 3.1 2.8 3.0 2.6 4.1 4.1 4.3 4.5 4.9 5.5 3.9 4.0 4.6
Std Deviation (1e-4) 0.4 0.4 1.3 1.4 1.2 1.1 0.8 1.2 1.5 1.4 1.3 1.0 0.9 1.1 1.5 1.4 1.4 1.4 0.9 1.5 1.6
Intensity Change (Log2) – – – 0.01 -0.02 -0.2 -0.37 -0.2 -0.24 -0.39 -0.31 -0.5 0.15 0.15 0.23 0.28 0.42 0.59 0.09 0.13 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 0.8 -0.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.3891 -0.882 -1.1235 -1.1142 -0.9566 -0.923 0.2122 -0.1701 -0.433 -0.5462 -0.3911 -0.4801 0.8208 0.653 0.4116 0.1922 0.3194 0.2861
Actin 0.0296 0.0002 0.0202 0.0263 0.0457 0.0021 0.0515 0.007 0.0139 0 0.0177 0.0034 0.0841 0.0002 0.0014 0.0017 0.0624 0.0081
Bud 0.0003 0.0001 0.0003 0.0001 0.0005 0.0001 0.0019 0.0003 0.0003 0 0.0008 0.0001 0.0012 0.0002 0 0.001 0.0001 0.0005
Bud Neck 0.0015 0.0002 0.0006 0.0003 0.0011 0.0014 0.0031 0.0003 0.0007 0 0.0002 0.0007 0.0067 0.0001 0.0001 0.0002 0.001 0.0013
Bud Periphery 0.0005 0.0001 0.0004 0.0001 0.0012 0.0001 0.0022 0.001 0.0005 0 0.0014 0.0001 0.002 0.0003 0 0.002 0.0001 0.0016
Bud Site 0.0026 0.007 0.0007 0.0006 0.0007 0.0001 0.0123 0.0038 0.0096 0 0.0003 0.0001 0.0049 0.0011 0.0003 0.0004 0.0002 0.0001
Cell Periphery 0.0003 0.0001 0.0001 0 0.0002 0 0.0003 0.0002 0.0006 0 0 0 0.0005 0.0001 0 0.0002 0 0
Cytoplasm 0.0962 0.1583 0.1277 0.2011 0.1461 0.2011 0.0364 0.1081 0.0699 0.0084 0.0474 0.1284 0.0683 0.144 0.1516 0.1395 0.1523 0.1534
Cytoplasmic Foci 0.0277 0.0155 0.0226 0.0138 0.0265 0.0486 0.0425 0.0214 0.0162 0.0004 0.0054 0.0207 0.0401 0.0267 0.0146 0.0142 0.0175 0.0244
Eisosomes 0.0008 0.0001 0.0003 0.0001 0.0009 0.0002 0.0009 0.0006 0.0007 0.0001 0.0002 0.0001 0.0027 0.0001 0.0001 0.0002 0.0003 0.0001
Endoplasmic Reticulum 0.0034 0.0053 0.0022 0.0037 0.0055 0.0023 0.0032 0.0035 0.0024 0.0001 0.0004 0.0021 0.0046 0.002 0.0025 0.0025 0.0012 0.0016
Endosome 0.0113 0.0128 0.0028 0.0103 0.0179 0.0062 0.0189 0.002 0.0101 0 0.004 0.006 0.0218 0.0019 0.007 0.0081 0.0043 0.0049
Golgi 0.0033 0.0006 0.0015 0.0051 0.0099 0.0005 0.011 0.0005 0.0054 0 0.0109 0.0059 0.0084 0.0002 0.0004 0.0005 0.0032 0.0011
Lipid Particles 0.0084 0.0039 0.0012 0.0044 0.0252 0.0028 0.0272 0.0024 0.0043 0 0.0011 0.0024 0.024 0.0007 0.0009 0.0009 0.0039 0.0066
Mitochondria 0.0029 0.0003 0.0087 0.0011 0.0053 0.0006 0.0107 0.0006 0.0105 0 0.0289 0.0027 0.0082 0.0007 0.0003 0.0028 0.0005 0.0029
None 0.7853 0.7748 0.7778 0.7137 0.6753 0.6738 0.6863 0.8357 0.8309 0.9907 0.8724 0.7997 0.6341 0.792 0.7889 0.8087 0.7141 0.7632
Nuclear Periphery 0.0056 0.005 0.0029 0.0033 0.0167 0.0029 0.0146 0.0011 0.0028 0.0001 0.0009 0.002 0.007 0.0013 0.0024 0.0019 0.0024 0.0026
Nucleolus 0.0016 0.0007 0.0006 0.0005 0.0008 0.0009 0.0013 0.0005 0.0004 0 0.0003 0.0002 0.0011 0.0008 0.0003 0.0005 0.0004 0.0005
Nucleus 0.0067 0.0047 0.0094 0.0072 0.0054 0.0303 0.0055 0.0035 0.0013 0 0.001 0.0163 0.003 0.0088 0.0183 0.0038 0.0179 0.0057
Peroxisomes 0.0042 0.0002 0.0056 0.002 0.0043 0.0093 0.0296 0.0037 0.002 0 0.0047 0.003 0.0612 0.0027 0.0009 0.0038 0.0054 0.0083
Punctate Nuclear 0.0054 0.0084 0.0138 0.0045 0.0074 0.0157 0.0371 0.0026 0.016 0.0001 0.0014 0.0053 0.0139 0.0149 0.0092 0.0021 0.012 0.0123
Vacuole 0.0018 0.0014 0.0006 0.0014 0.0016 0.0008 0.0024 0.0013 0.0011 0 0.0004 0.0006 0.0013 0.0009 0.0007 0.0046 0.0005 0.0007
Vacuole Periphery 0.0007 0.0002 0.0002 0.0003 0.0017 0.0002 0.0012 0.0001 0.0003 0 0.0003 0.0001 0.0008 0.0001 0.0001 0.0006 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.3533 5.63 5.8488 7.0727 7.7573 6.9239 7.8272 9.6051 7.7212 8.1029
Translational Efficiency 1.243 1.0306 1.0615 0.8339 0.7655 0.852 0.7359 0.5706 0.7641 0.9235

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein of unknown function; contains a J-domain, which is a region with homology to the E. coli DnaJ protein
Localization
Cell Percentages cytoplasm (35%), nucleus (4%), mixed (49%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Jjj2

Jjj2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Jjj2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available