Standard name
Human Ortholog
Description cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; phosphorylates and inhibits Whi3p to promote G1/S phase passage; partially redundant with Tpk2p and Tpk3p; phosphorylates pre-Tom40p, which impairs its import into mitochondria under non-respiratory conditions; TPK1 has a paralog, TPK3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.07
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.77 0.57 0.23 0.22 0.35 0.57 0.49 0.49 0.48 0.56 0.49 0.59 0.11 0.22 0.2 0.18 0.09 0.12 0.68 0.46 0.16 0.16 0.15 0.12
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0 0 0.13 0.05 0.07 0.12 0.09 0.18 0.21 0.22 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0.07 0.39 0.31 0.14 0.09 0.13 0.14 0.13 0.11 0.11 0.06 0.5 0.55 0.57 0.36 0.32 0.31 0.14 0.38 0.6 0.54 0.59 0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0
Vac/Vac Membrane 0.18 0.4 0.49 0.47 0.51 0.36 0.35 0.37 0.33 0.26 0.32 0.27 0.42 0.3 0.29 0.5 0.61 0.57 0.08 0.07 0.14 0.11 0.1 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 1 0 2 0 0 1 0 0 0 0 0 0 0 0 0
Bud 0 0 0 2 0 1 3 4 3 7 8 5 0 0 0 0 0 0 5 3 5 3 15 18
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 3
Bud Site 0 0 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 2 1 2 6 3 6 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 116 52 42 52 130 196 332 259 252 406 237 366 24 79 90 22 13 14 121 52 35 9 48 33
Endoplasmic Reticulum 0 4 1 0 1 0 2 2 3 5 0 7 0 0 2 4 10 4 0 0 0 0 1 0
Endosome 0 1 3 7 28 7 9 7 5 1 2 1 21 9 15 3 2 3 3 1 3 1 2 3
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 3 5 2 1 0 3 0 1 1
Mitochondria 19 3 3 32 17 25 83 49 94 154 108 96 1 0 0 0 2 2 2 0 1 0 2 1
Nucleus 3 6 70 75 53 32 91 72 68 78 55 37 104 200 252 44 46 35 24 44 135 31 183 165
Nuclear Periphery 0 0 0 1 1 1 1 1 3 1 2 2 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 1 0 0 0 0 1 5 3 0 1 0 0 0 0 0 1 1 0 0 0 1 0 3 8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 1 5 3 14 10
Vac/Vac Membrane 27 37 88 113 190 123 236 192 174 191 156 168 88 109 129 62 86 65 15 8 31 6 31 24
Unique Cell Count 150 92 181 240 375 342 678 526 524 729 488 617 210 361 442 123 142 114 179 116 227 59 314 275
Labelled Cell Count 166 103 208 282 420 387 765 590 604 853 571 691 240 397 489 140 165 125 179 116 227 59 314 275


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 6.8 6.4 5.9 6.8 7.4 7.7 8.0 7.9 8.0 8.1 8.6 7.8 8.2 8.6 14.2 15.1 14.8 6.5 7.9 8.5
Std Deviation (1e-4) 0.8 1.5 1.4 1.5 1.7 1.5 1.8 2.1 2.1 2.3 2.6 2.8 1.1 1.5 1.6 3.0 3.0 2.9 0.9 1.4 1.5
Intensity Change (Log2) – – – -0.1 0.1 0.22 0.27 0.34 0.3 0.32 0.35 0.44 0.29 0.36 0.44 1.16 1.25 1.21 0.04 0.32 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 2.7 7.5 6.2 6.1 5.9 7.8 5.9 8.5 -3.1 -0.3 -0.8 -1.1 -3.3 -2.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.8 0 0 0 0 0 0 0 3.4 0 1.2 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.3 1.7 2.7 4.2 3.4 5.5 6.2 6.3 5.0 0 0 0 0 0 0
Nucleus -1.6 -6.5 -8.0 -7.7 -7.2 -7.5 -9.1 -8.1 -11.3 2.2 3.7 4.2 -0.5 -1.2 -1.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.3 0.5 -2.8 -3.4 -2.9 -3.7 -5.9 -4.0 -5.4 -1.3 -4.2 -4.6 0.3 2.1 1.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.6517 6.9538 5.9642 5.4307 6.1491 6.2026 2.5018 3.6775 3.6062 2.6299 2.9611 3.4723 3.2718 3.8612 3.2299 3.0178 2.6884 3.3077
Actin 0.1002 0.0003 0.0032 0.0098 0.0134 0.0019 0.046 0.0006 0.0164 0 0.0059 0.0006 0.0232 0.001 0.0039 0.0082 0.0017 0.0001
Bud 0.0028 0.0061 0.0091 0.0033 0.001 0.0003 0.0007 0.0005 0.0004 0.0001 0.0006 0.0001 0.0005 0.0005 0.0002 0.0002 0.0004 0.0002
Bud Neck 0.0098 0.0006 0.0006 0.0037 0.0009 0.0027 0.0033 0.0004 0.0003 0.0002 0.0031 0.0011 0.0039 0.0005 0.0003 0.0021 0.0007 0.0016
Bud Periphery 0.0042 0.0008 0.0027 0.002 0.0014 0.0002 0.0008 0.0002 0.0003 0 0.0004 0.0001 0.001 0.0002 0.0001 0.0002 0.0012 0.0001
Bud Site 0.009 0.0014 0.0012 0.0029 0.0025 0.0003 0.0036 0.0013 0.0026 0 0.0025 0.0001 0.0019 0.0013 0.0002 0.0018 0.0004 0.0001
Cell Periphery 0.0022 0.0001 0.0001 0.0001 0.0002 0 0.0006 0.0001 0 0 0.0001 0 0.0003 0.0001 0 0.0002 0.0001 0
Cytoplasm 0.1058 0.1902 0.0933 0.0355 0.2153 0.1044 0.0953 0.2308 0.1149 0.0336 0.0819 0.0572 0.0508 0.2157 0.1129 0.0418 0.1052 0.0835
Cytoplasmic Foci 0.0481 0.001 0.0023 0.0017 0.0058 0.0099 0.0264 0.0046 0.0083 0 0.0171 0.0004 0.0013 0.0013 0.0057 0.0066 0.0004 0.0003
Eisosomes 0.0037 0 0 0 0.0002 0 0.0005 0 0 0 0.0001 0 0.0002 0 0 0.0003 0.0001 0
Endoplasmic Reticulum 0.0106 0.0019 0.0013 0.0072 0.0077 0.0015 0.011 0.0022 0.0006 0.0001 0.0029 0.0004 0.0108 0.0026 0.0009 0.0062 0.001 0.0007
Endosome 0.0557 0.0012 0.0093 0.0047 0.0344 0.0088 0.0367 0.0089 0.0039 0.0001 0.023 0.0014 0.0098 0.0027 0.0024 0.028 0.0031 0.0006
Golgi 0.0194 0 0.0024 0.0004 0.0043 0.0069 0.0134 0.0005 0.0019 0 0.0193 0.0032 0.0037 0.0004 0.0022 0.0025 0.0007 0
Lipid Particles 0.0285 0.0001 0.006 0.0001 0.001 0.0078 0.0208 0.0009 0.0026 0 0.0128 0.0051 0.0024 0.0002 0.0011 0.0142 0.0021 0
Mitochondria 0.0212 0.0009 0.0009 0.0036 0.1034 0.0063 0.0285 0.0007 0.0019 0.0002 0.0181 0.0134 0.0021 0.0036 0.0008 0.0047 0.0442 0.0002
None 0.0158 0.0031 0.0033 0.0023 0.0032 0.0018 0.0266 0.0205 0.0018 0.0001 0.0008 0.0001 0.0007 0.0017 0.0016 0.0254 0.0006 0.0003
Nuclear Periphery 0.0217 0.0136 0.012 0.0427 0.0741 0.0096 0.0234 0.0122 0.0063 0.0091 0.0135 0.0101 0.0351 0.0199 0.012 0.0541 0.0181 0.015
Nucleolus 0.0039 0.0014 0.001 0.0018 0.0008 0.0018 0.0023 0.0016 0.0013 0.0005 0.0019 0.0006 0.001 0.002 0.0012 0.0024 0.003 0.001
Nucleus 0.4477 0.77 0.8431 0.8578 0.5158 0.8131 0.6226 0.7029 0.8298 0.9551 0.7457 0.9031 0.8421 0.7337 0.8459 0.7751 0.8088 0.8883
Peroxisomes 0.0256 0.0001 0.0008 0.0004 0.0016 0.0029 0.0164 0.0003 0.0028 0 0.0079 0.0007 0.0005 0.0002 0.0007 0.0016 0.0007 0
Punctate Nuclear 0.0425 0.0051 0.0045 0.0174 0.0077 0.018 0.0107 0.0051 0.0027 0.0006 0.0402 0.0011 0.0039 0.0097 0.0071 0.0169 0.0034 0.0068
Vacuole 0.0182 0.0017 0.0026 0.0014 0.0017 0.0014 0.0074 0.005 0.0009 0.0002 0.0014 0.0007 0.0032 0.0022 0.0006 0.0038 0.0017 0.0009
Vacuole Periphery 0.0036 0.0003 0.0005 0.0009 0.0035 0.0003 0.0029 0.0008 0.0002 0.0001 0.0007 0.0004 0.0016 0.0005 0.0002 0.0034 0.0024 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.9377 4.9567 6.7431 25.6545 13.1798 25.8612 18.585 26.4966 40.1688 24.369
Translational Efficiency 0.9698 1.7617 2.2547 1.0616 1.2772 1.3154 0.9795 1.0602 0.9909 1.0181

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; phosphorylates and inhibits Whi3p to promote G1/S phase passage; partially redundant with Tpk2p and Tpk3p; phosphorylates pre-Tom40p, which impairs its import into mitochondria under non-respiratory conditions; TPK1 has a paralog, TPK3, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (56%), nucleus (10%), mixed (32%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Tpk1

Tpk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tpk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available