Standard name
Human Ortholog
Description Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0.07 0.05 0 0.07 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0.08 0.12 0.11 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0.13 0.12 0.14 0 0 0 0 0.05 0 0.09 0.12 0.16
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05
Mitochondria 0.06 0 0.11 0.11 0.06 0.15 0.15 0.22 0.24 0.17 0.21 0.08 0 0 0 0.05 0.05 0 0 0 0.08 0 0.05
Nucleus 0.61 0.6 0.61 0.62 0.52 0.52 0.34 0.34 0.38 0.39 0.37 0.33 0.43 0.37 0.58 0.65 0.64 0.55 0.45 0.43 0.19 0.16 0.1
Nuclear Periphery 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.35 0.35 0.3 0.3 0.33 0.38 0.42 0.43 0.4 0.43 0.46 0.26 0.24 0.25 0.17 0.14 0.11 0.15 0.15 0.19 0 0.05 0.06
Peroxisomes 0 0 0 0 0 0 0.07 0.07 0.06 0 0.08 0.18 0.13 0.15 0 0 0 0 0 0 0.12 0.15 0.12
SpindlePole 0 0 0 0 0.08 0 0.09 0.07 0.07 0.09 0.09 0.19 0.15 0.2 0 0 0 0.12 0.11 0.09 0.21 0.23 0.2
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.09 0.11 0.07 0.08 0.09 0.12 0.12 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 2 4 6
Bud 0 0 3 3 0 0 2 2 2 4 0 1 2 1 0 0 0 2 6 3 5 3 4
Bud Neck 0 1 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 6 3 1 1 2
Bud Site 0 1 2 1 4 5 6 14 11 8 16 2 2 2 0 0 0
Cell Periphery 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 2 6 6 7 4 8 5 1 3 2 7 23 19 28 14 15 0 0 0 1 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 1 0 0 0 0 0 0 0 3 3 4 0 0 0 0 0 1
Endosome 3 4 4 3 8 2 6 1 1 3 1 20 30 33 5 2 3 5 6 5 17 24 35
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 7 0 2 0 0 4 6 10 11
Mitochondria 8 1 18 18 11 33 32 47 53 32 48 12 7 8 7 7 7 1 1 5 15 8 10
Nucleus 80 61 104 104 97 113 71 72 83 71 85 51 108 89 132 83 84 81 58 81 37 32 22
Nuclear Periphery 1 6 3 3 1 6 2 2 5 1 0 2 1 2 0 1 0 1 1 3 0 1 1
Nucleolus 46 35 51 51 62 82 88 89 88 79 106 40 60 59 39 18 14 22 19 36 8 9 13
Peroxisomes 2 1 6 4 5 8 15 15 12 7 19 27 34 37 3 1 1 0 2 8 23 30 25
SpindlePole 4 3 7 7 15 2 19 15 15 16 22 29 39 48 4 1 3 17 14 17 40 46 44
Vac/Vac Membrane 2 3 1 1 3 2 3 2 3 4 2 4 6 8 26 12 14 10 10 17 23 24 31
Unique Cell Count 131 101 170 169 188 216 211 209 218 184 232 153 254 240 227 128 131 147 132 191 190 204 217
Labelled Cell Count 149 120 205 201 213 257 253 264 277 228 301 195 314 306 254 142 148 147 132 191 190 204 217


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.3 16.5 13.0 13.0 16.8 15.0 15.6 14.6 14.0 14.7 14.5 16.1 16.1 16.5 22.6 21.4 20.0 14.1 14.6 14.0
Std Deviation (1e-4) 5.4 6.8 5.3 5.3 7.0 5.5 6.1 5.6 6.1 6.1 7.1 8.7 9.2 7.4 10.4 10.1 9.1 7.6 6.4 8.6
Intensity Change (Log2) 0.0 0.37 0.2 0.26 0.16 0.11 0.17 0.15 0.3 0.3 0.34 0.79 0.72 0.62 0.12 0.16 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 2.7 2.1 0 2.7 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.1 0 0.1 0 0 0 0 0.5 2.2 1.8 3.1 2.5 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 1.0 0 0 0 0 0 0 3.7 3.5 4.0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -1.6 1.4 1.3 3.1 3.5 1.8 2.7 -0.8 -3.4 -3.0 -3.0 -1.6 -1.6
Nucleus 0.1 -1.8 -1.7 -5.4 -5.2 -4.5 -4.2 -4.9 -5.0 -3.8 -4.8 -0.6 0.6 0.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.6 1.6 2.4 2.5 2.1 2.5 3.2 -0.8 -1.5 -1.2 -3.0 -3.2 -4.0
Peroxisomes 0 -0.5 0.1 1.5 1.5 0.9 0.1 1.9 4.2 3.4 3.9 0 0 0
SpindlePole 0 1.5 0 1.9 1.3 1.2 1.7 2.1 4.2 3.6 4.6 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 4.3 3.7 4.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.0004 0.0036 0.0003 0.0097 0.0001 0.0059
Bud 0.0002 0.0008 0.0001 0.001 0.0005 0.0013
Bud Neck 0.0007 0.0009 0.0002 0.0014 0.0008 0.0266
Bud Periphery 0.0006 0.0018 0.0003 0.0013 0.0009 0.0053
Bud Site 0.001 0.0016 0.0002 0.0023 0.0013 0.0008
Cell Periphery 0.0003 0.0002 0.0001 0.0002 0.0002 0.0007
Cytoplasm 0.0001 0.0004 0.0001 0.0003 0.0001 0.0007
Cytoplasmic Foci 0.0171 0.0008 0.0052 0.0478 0.0012 0.0673
Eisosomes 0 0.0001 0 0.0002 0 0.0001
Endoplasmic Reticulum 0 0.0002 0 0.0001 0 0.0008
Endosome 0.0012 0.0008 0.0002 0.0012 0.0002 0.0031
Golgi 0.0062 0.0004 0.0004 0.0055 0.0002 0.001
Lipid Particles 0.1293 0.0008 0.0415 0.1135 0.0013 0.0321
Mitochondria 0.0012 0.0166 0.0081 0.1294 0.0023 0.012
None 0.0002 0.0004 0.0002 0.0003 0.0001 0.0004
Nuclear Periphery 0.0092 0.0393 0.0231 0.0176 0.0004 0.0066
Nucleolus 0.3052 0.2647 0.1618 0.1154 0.5444 0.2452
Nucleus 0.4601 0.6101 0.5647 0.195 0.43 0.5317
Peroxisomes 0.0142 0.0022 0.0013 0.1587 0.0004 0.0037
Punctate Nuclear 0.0507 0.0516 0.1865 0.1969 0.015 0.0481
Vacuole 0.0009 0.0007 0.0006 0.0007 0.0005 0.004
Vacuole Periphery 0.001 0.0021 0.0051 0.0015 0.0003 0.0026

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 137.5518 85.5394 45.6219 60.3115 91.1094 167.3616 76.3534 62.9346 62.846 127.0226
Translational Efficiency 2.5008 2.7031 2.0971 2.2386 2.9421 2.4601 2.1946 1.6883 2.4045 2.4502

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages N/A
Cell Cycle Regulation N/A
Subcompartmental Group N/A

Rfa3

Rfa3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rfa3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available