Standard name | |
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Human Ortholog | |
Description | Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 4 | 6 |
Bud | 0 | 0 | 3 | 3 | 0 | 0 | – | 2 | 2 | 2 | 4 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 2 | 6 | 3 | 5 | 3 | 4 |
Bud Neck | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 3 | 1 | 1 | 2 |
Bud Site | 0 | 1 | 2 | 1 | 4 | 5 | – | 6 | 14 | 11 | 8 | 16 | 2 | 2 | 2 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 2 | 6 | 6 | 7 | 4 | – | 8 | 5 | 1 | 3 | 2 | 7 | 23 | 19 | 28 | 14 | 15 | 0 | 0 | 0 | 1 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 3 | 4 | 4 | 3 | 8 | 2 | – | 6 | 1 | 1 | 3 | 1 | 20 | 30 | 33 | 5 | 2 | 3 | 5 | 6 | 5 | 17 | 24 | 35 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 0 | 2 | 0 | 0 | 4 | 6 | 10 | 11 |
Mitochondria | 8 | 1 | 18 | 18 | 11 | 33 | – | 32 | 47 | 53 | 32 | 48 | 12 | 7 | 8 | 7 | 7 | 7 | 1 | 1 | 5 | 15 | 8 | 10 |
Nucleus | 80 | 61 | 104 | 104 | 97 | 113 | – | 71 | 72 | 83 | 71 | 85 | 51 | 108 | 89 | 132 | 83 | 84 | 81 | 58 | 81 | 37 | 32 | 22 |
Nuclear Periphery | 1 | 6 | 3 | 3 | 1 | 6 | – | 2 | 2 | 5 | 1 | 0 | 2 | 1 | 2 | 0 | 1 | 0 | 1 | 1 | 3 | 0 | 1 | 1 |
Nucleolus | 46 | 35 | 51 | 51 | 62 | 82 | – | 88 | 89 | 88 | 79 | 106 | 40 | 60 | 59 | 39 | 18 | 14 | 22 | 19 | 36 | 8 | 9 | 13 |
Peroxisomes | 2 | 1 | 6 | 4 | 5 | 8 | – | 15 | 15 | 12 | 7 | 19 | 27 | 34 | 37 | 3 | 1 | 1 | 0 | 2 | 8 | 23 | 30 | 25 |
SpindlePole | 4 | 3 | 7 | 7 | 15 | 2 | – | 19 | 15 | 15 | 16 | 22 | 29 | 39 | 48 | 4 | 1 | 3 | 17 | 14 | 17 | 40 | 46 | 44 |
Vac/Vac Membrane | 2 | 3 | 1 | 1 | 3 | 2 | – | 3 | 2 | 3 | 4 | 2 | 4 | 6 | 8 | 26 | 12 | 14 | 10 | 10 | 17 | 23 | 24 | 31 |
Unique Cell Count | 131 | 101 | 170 | 169 | 188 | 216 | 211 | 209 | 218 | 184 | 232 | 153 | 254 | 240 | 227 | 128 | 131 | 147 | 132 | 191 | 190 | 204 | 217 | |
Labelled Cell Count | 149 | 120 | 205 | 201 | 213 | 257 | 253 | 264 | 277 | 228 | 301 | 195 | 314 | 306 | 254 | 142 | 148 | 147 | 132 | 191 | 190 | 204 | 217 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.3 | 16.5 | 13.0 | 13.0 | 16.8 | 15.0 | – | 15.6 | 14.6 | 14.0 | 14.7 | 14.5 | 16.1 | 16.1 | 16.5 | 22.6 | 21.4 | 20.0 | 14.1 | 14.6 | 14.0 |
Std Deviation (1e-4) | 5.4 | 6.8 | 5.3 | 5.3 | 7.0 | 5.5 | – | 6.1 | 5.6 | 6.1 | 6.1 | 7.1 | 8.7 | 9.2 | 7.4 | 10.4 | 10.1 | 9.1 | 7.6 | 6.4 | 8.6 |
Intensity Change (Log2) | – | – | – | 0.0 | 0.37 | 0.2 | – | 0.26 | 0.16 | 0.11 | 0.17 | 0.15 | 0.3 | 0.3 | 0.34 | 0.79 | 0.72 | 0.62 | 0.12 | 0.16 | 0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 2.7 | 2.1 | 0 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0.1 | 0 | – | 0.1 | 0 | 0 | 0 | 0 | 0.5 | 2.2 | 1.8 | 3.1 | 2.5 | 2.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1.0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.7 | 3.5 | 4.0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | -1.6 | 1.4 | – | 1.3 | 3.1 | 3.5 | 1.8 | 2.7 | -0.8 | -3.4 | -3.0 | -3.0 | -1.6 | -1.6 |
Nucleus | 0.1 | -1.8 | -1.7 | – | -5.4 | -5.2 | -4.5 | -4.2 | -4.9 | -5.0 | -3.8 | -4.8 | -0.6 | 0.6 | 0.5 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0.6 | 1.6 | – | 2.4 | 2.5 | 2.1 | 2.5 | 3.2 | -0.8 | -1.5 | -1.2 | -3.0 | -3.2 | -4.0 |
Peroxisomes | 0 | -0.5 | 0.1 | – | 1.5 | 1.5 | 0.9 | 0.1 | 1.9 | 4.2 | 3.4 | 3.9 | 0 | 0 | 0 |
SpindlePole | 0 | 1.5 | 0 | – | 1.9 | 1.3 | 1.2 | 1.7 | 2.1 | 4.2 | 3.6 | 4.6 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.3 | 3.7 | 4.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0004 | 0.0036 | 0.0003 | 0.0097 | 0.0001 | 0.0059 | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | 0.0002 | 0.0008 | 0.0001 | 0.001 | 0.0005 | 0.0013 | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | 0.0007 | 0.0009 | 0.0002 | 0.0014 | 0.0008 | 0.0266 | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Periphery | 0.0006 | 0.0018 | 0.0003 | 0.0013 | 0.0009 | 0.0053 | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | 0.001 | 0.0016 | 0.0002 | 0.0023 | 0.0013 | 0.0008 | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0007 | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0007 | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0171 | 0.0008 | 0.0052 | 0.0478 | 0.0012 | 0.0673 | – | – | – | – | – | – | – | – | – | – | – | – |
Eisosomes | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0008 | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | 0.0012 | 0.0008 | 0.0002 | 0.0012 | 0.0002 | 0.0031 | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | 0.0062 | 0.0004 | 0.0004 | 0.0055 | 0.0002 | 0.001 | – | – | – | – | – | – | – | – | – | – | – | – |
Lipid Particles | 0.1293 | 0.0008 | 0.0415 | 0.1135 | 0.0013 | 0.0321 | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | 0.0012 | 0.0166 | 0.0081 | 0.1294 | 0.0023 | 0.012 | – | – | – | – | – | – | – | – | – | – | – | – |
None | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | 0.0092 | 0.0393 | 0.0231 | 0.0176 | 0.0004 | 0.0066 | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | 0.3052 | 0.2647 | 0.1618 | 0.1154 | 0.5444 | 0.2452 | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | 0.4601 | 0.6101 | 0.5647 | 0.195 | 0.43 | 0.5317 | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | 0.0142 | 0.0022 | 0.0013 | 0.1587 | 0.0004 | 0.0037 | – | – | – | – | – | – | – | – | – | – | – | – |
Punctate Nuclear | 0.0507 | 0.0516 | 0.1865 | 0.1969 | 0.015 | 0.0481 | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | 0.0009 | 0.0007 | 0.0006 | 0.0007 | 0.0005 | 0.004 | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole Periphery | 0.001 | 0.0021 | 0.0051 | 0.0015 | 0.0003 | 0.0026 | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 137.5518 | 85.5394 | 45.6219 | 60.3115 | 91.1094 | 167.3616 | 76.3534 | 62.9346 | 62.846 | 127.0226 |
Translational Efficiency | 2.5008 | 2.7031 | 2.0971 | 2.2386 | 2.9421 | 2.4601 | 2.1946 | 1.6883 | 2.4045 | 2.4502 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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