Standard name | |
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Human Ortholog | |
Description | Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | – | 0 | 0 | 0 | – | 1 | 1 | 1 | 1 | 1 | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | – | 0 | 0 | 0 | – | 1 | 0 | 1 | 0 | 2 | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | – | 2 | 1 | 6 | 3 | 6 | – | – | – | 0 | – | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 0 | – | 10 | 12 | 8 | – | 30 | 8 | 9 | 9 | 13 | – | – | – | 4 | – | 4 | 0 | 0 | 0 | 0 | 1 | 0 |
Endoplasmic Reticulum | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | – | – | – | 1 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | – | 0 | 0 | 1 | – | 1 | 2 | 3 | 0 | 1 | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 41 | 10 | – | 68 | 69 | 44 | – | 51 | 49 | 62 | 56 | 53 | – | – | – | 11 | – | 10 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleus | 38 | 1 | – | 11 | 6 | 6 | – | 14 | 9 | 4 | 8 | 16 | – | – | – | 13 | – | 17 | 34 | 15 | 7 | 0 | 1 | 2 |
Nuclear Periphery | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 78 | 19 | – | 87 | 111 | 77 | – | 183 | 112 | 134 | 112 | 130 | – | – | – | 32 | – | 33 | 45 | 29 | 29 | 0 | 13 | 12 |
Peroxisomes | 0 | 0 | – | 0 | 1 | 1 | – | 2 | 2 | 1 | 3 | 8 | – | – | – | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | – | 0 | 0 | 2 | – | 8 | 5 | 8 | 9 | 15 | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | – | 0 | 1 | 0 | – | 5 | 2 | 2 | 2 | 2 | – | – | – | 4 | – | 0 | 3 | 2 | 1 | 1 | 1 | 1 |
Unique Cell Count | 90 | 19 | 102 | 132 | 94 | 232 | 136 | 166 | 140 | 173 | 51 | 50 | 86 | 51 | 41 | 5 | 21 | 23 | ||||||
Labelled Cell Count | 158 | 30 | 176 | 200 | 139 | 298 | 191 | 231 | 203 | 248 | 65 | 68 | 86 | 51 | 41 | 5 | 21 | 23 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 61.0 | 54.9 | – | 51.1 | 43.7 | 25.4 | – | 18.9 | 18.4 | 17.2 | 16.7 | 15.7 | – | – | – | 84.9 | – | 94.2 | – | – | – |
Std Deviation (1e-4) | 17.9 | 16.0 | – | 24.8 | 26.3 | 12.7 | – | 9.2 | 10.1 | 9.0 | 11.1 | 8.6 | – | – | – | 43.0 | – | 52.8 | – | – | – |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 93.988 | 128.7436 | 105.9081 | 103.6699 | 64.0707 | 106.7181 | 113.1376 | 127.0214 | 112.4143 | 103.4738 | 88.678 | 98.0622 | 122.8817 | 153.409 | 134.6675 | 138.8847 | 76.6167 | 112.8774 |
Actin | 0.0001 | 0 | 0 | 0 | 0.0003 | 0.0151 | 0.0001 | 0 | 0 | 0 | 0.001 | 0 | 0.0048 | 0 | 0 | 0 | 0.006 | 0.0058 |
Bud | 0.0001 | 0 | 0 | 0 | 0.0003 | 0.0008 | 0.0001 | 0 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0007 | 0.0002 |
Bud Neck | 0 | 0 | 0 | 0 | 0.0003 | 0.0004 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0003 | 0.0005 |
Bud Periphery | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0012 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0008 | 0.0005 |
Bud Site | 0.0002 | 0 | 0 | 0 | 0.0004 | 0.0011 | 0.0004 | 0 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0 | 0 | 0.0012 | 0.0003 |
Cell Periphery | 0.0005 | 0.0007 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0004 | 0.0004 | 0.0007 | 0.0008 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0043 | 0.0007 |
Cytoplasm | 0 | 0.0311 | 0 | 0 | 0.0002 | 0.0006 | 0.0002 | 0 | 0 | 0 | 0.0005 | 0 | 0.0003 | 0.0163 | 0 | 0 | 0.0022 | 0.0004 |
Cytoplasmic Foci | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.003 | 0.001 | 0 | 0 | 0 | 0.0026 | 0 | 0.0032 | 0.0147 | 0 | 0 | 0.0139 | 0.0013 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0.0011 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0022 | 0.0025 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0.0051 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0016 | 0 | 0 | 0 | 0.0401 | 0.0259 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0.0023 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0041 | 0.0001 | 0 | 0 | 0.0082 | 0.0107 |
Lipid Particles | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0049 | 0.0012 | 0 | 0 | 0 | 0.0008 | 0.0001 | 0.0261 | 0.0009 | 0 | 0 | 0.0291 | 0.0016 |
Mitochondria | 0.0088 | 0.0064 | 0.0046 | 0.0031 | 0.0051 | 0.0131 | 0.0059 | 0.0042 | 0.0029 | 0.0132 | 0.0957 | 0.0036 | 0.0386 | 0.0048 | 0.0014 | 0.0063 | 0.0223 | 0.0227 |
None | 0.0002 | 0 | 0 | 0 | 0.0003 | 0.002 | 0.0003 | 0 | 0 | 0.0001 | 0.0026 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0054 | 0.0003 |
Nuclear Periphery | 0.0006 | 0.0001 | 0.0001 | 0 | 0.001 | 0.0121 | 0.0064 | 0.0001 | 0.0003 | 0.0004 | 0.0029 | 0.0001 | 0.0009 | 0.001 | 0.0001 | 0 | 0.038 | 0.0316 |
Nucleolus | 0.6612 | 0.6111 | 0.828 | 0.9396 | 0.4139 | 0.7056 | 0.7418 | 0.6556 | 0.5756 | 0.7483 | 0.7506 | 0.6064 | 0.4388 | 0.5367 | 0.7257 | 0.743 | 0.6725 | 0.6337 |
Nucleus | 0.3249 | 0.3475 | 0.1647 | 0.0565 | 0.5593 | 0.2172 | 0.2353 | 0.3374 | 0.4183 | 0.2344 | 0.128 | 0.388 | 0.4454 | 0.4211 | 0.2718 | 0.2496 | 0.1026 | 0.2488 |
Peroxisomes | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0018 | 0.0002 | 0 | 0 | 0.0001 | 0.0006 | 0 | 0.0292 | 0.0006 | 0 | 0 | 0.0049 | 0.0002 |
Punctate Nuclear | 0.0003 | 0 | 0 | 0 | 0.0177 | 0.0081 | 0.0036 | 0 | 0 | 0.0001 | 0.0045 | 0.0001 | 0.0024 | 0.0017 | 0 | 0 | 0.0274 | 0.0009 |
Vacuole | 0.0006 | 0.0009 | 0.0004 | 0.0002 | 0.0001 | 0.0011 | 0.0007 | 0.0004 | 0.0007 | 0.0007 | 0.0008 | 0.0003 | 0.0006 | 0.0003 | 0.0002 | 0.0001 | 0.007 | 0.0023 |
Vacuole Periphery | 0.002 | 0.002 | 0.0017 | 0.0004 | 0.0002 | 0.0031 | 0.0016 | 0.0018 | 0.0016 | 0.0017 | 0.0063 | 0.0007 | 0.0021 | 0.0011 | 0.0005 | 0.0007 | 0.0108 | 0.0089 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2241.9044 | 2241.3077 | 2538.4875 | 2474.369 | 2440.7771 | 2138.241 | 2072.6827 | 1872.9358 | 2033.9919 | 2029.304 |
Translational Efficiency | 1.9384 | 1.9949 | 1.9325 | 1.3199 | 2.0263 | 2.0074 | 1.9638 | 2.1296 | 1.8542 | 2.1683 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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