Standard name
Human Ortholog
Description Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.06 0 0 0.06 0 0.05 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.18 0.16 0.52 0.33 0.41 0.2 0.2 0.19 0.09 0.1 0.1 0.08 0.41 0.47 0.51 0.33 0.27 0.32 0 0 0.05 0.06 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0.25 0.05 0.11 0.08 0 0.06 0 0 0 0 0 0.26 0.21 0.18 0.13 0.2 0.24 0.14 0.16 0.16 0.08 0.11 0.11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.68 0.27 0 0.32 0.24 0.62 0.6 0.55 0.83 0.79 0.91 0.88 0.1 0.05 0 0.12 0.06 0.07 0.56 0.55 0.47 0.64 0.56 0.57
Nucleus 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.09 0 0.09 0 0 0 0 0 0.05 0 0 0.09 0.09 0 0.1 0.07 0.08 0 0 0.05 0 0.05 0.05
SpindlePole 0.13 0.14 0.31 0.21 0.22 0.15 0.19 0.21 0.13 0.13 0 0.08 0.19 0.22 0.29 0.37 0.38 0.37 0.08 0.08 0.12 0.06 0.08 0.07
Vac/Vac Membrane 0.1 0.21 0.09 0 0.06 0 0 0 0.05 0 0 0 0.1 0.06 0.06 0.06 0.09 0.09 0.07 0 0 0 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 2 3 2 2 1 1 2 0 2 1 3 4 4 2 0 0 3 8 6 3 7 15
Bud 6 11 5 14 5 7 20 9 13 21 25 16 2 4 2 7 8 8 1 3 6 1 3 6
Bud Neck 0 2 14 6 9 8 9 6 1 5 0 2 1 3 3 6 12 2 2 2 5 1 5 8
Bud Site 4 1 0 1 0 5 2 3 6 3 7 5 1 0 0 3 3 5
Cell Periphery 0 7 3 2 2 0 2 0 3 3 1 2 2 1 3 6 9 6 0 0 1 0 0 1
Cytoplasm 33 42 120 81 111 59 74 54 24 40 45 30 62 100 119 84 65 67 7 8 15 12 6 9
Endoplasmic Reticulum 4 2 1 1 2 7 0 2 3 1 1 2 1 5 1 13 7 7 0 1 6 1 1 3
Endosome 8 63 12 27 21 7 22 12 7 14 1 1 39 44 41 32 49 51 33 54 54 16 29 52
Golgi 1 2 2 1 0 0 0 0 0 0 0 0 0 0 0 4 5 1 2 6 5 1 3 6
Mitochondria 124 68 6 78 64 179 219 161 230 313 404 351 15 11 3 29 14 15 128 182 165 132 152 270
Nucleus 2 1 1 0 9 9 5 14 3 5 10 7 1 0 1 4 2 2 0 0 0 1 2 2
Nuclear Periphery 0 3 0 0 0 0 4 1 4 1 4 3 0 1 0 0 0 1 2 3 1 1 0 4
Nucleolus 1 0 0 1 0 2 1 2 1 1 0 3 0 1 1 0 1 2 1 1 2 2 2 2
Peroxisomes 2 24 4 23 6 7 8 4 2 19 2 2 13 20 5 24 17 18 7 10 17 5 13 24
SpindlePole 23 35 72 52 59 42 70 60 36 52 18 30 29 47 67 93 93 78 18 26 41 12 22 33
Vac/Vac Membrane 19 54 20 11 17 13 15 13 13 15 6 11 15 12 13 14 23 19 15 14 8 9 15 24
Unique Cell Count 183 256 230 246 268 289 362 291 276 394 445 398 152 213 233 252 243 212 232 334 349 207 274 475
Labelled Cell Count 228 316 262 301 307 347 452 342 348 493 526 466 184 253 263 321 308 282 232 334 349 207 274 475


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 5.1 5.4 4.9 5.3 4.3 4.4 4.9 3.8 4.1 3.7 3.8 5.4 5.4 5.3 6.1 6.7 6.0 5.4 5.4 5.5
Std Deviation (1e-4) 0.7 1.1 1.4 1.4 1.7 1.1 1.3 1.5 0.8 1.0 1.1 0.9 0.7 0.9 0.9 1.5 2.3 1.7 1.1 1.2 1.0
Intensity Change (Log2) -0.13 -0.03 -0.33 -0.28 -0.13 -0.49 -0.38 -0.56 -0.51 0.01 -0.01 -0.02 0.19 0.31 0.14 -0.0 0.0 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 2.0 0 0 0 0.6 0 0 0 0 0 0 0 0.4 0.7 1.0
Bud Neck -2.0 -1.4 0 0 -2.4 0 0 0 0 -2.7 -2.6 -2.7 -2.0 -0.5 -2.9
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1.7 0
Cytoplasm -4.2 -2.4 -7.6 -8.0 -8.1 -10.8 -11.6 -12.1 -12.6 -2.2 -1.1 -0.2 -4.2 -5.7 -4.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.1 0 0
Endosome 2.3 1.2 0 0 -0.6 0 0 0 0 5.7 4.9 4.2 2.8 4.8 5.7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 8.3 6.8 0 0 12.8 0 0 0 0 3.0 1.4 0 3.8 1.7 2.2
Nucleus 0 0 0 0 3.0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 3.6 0 0 0 0 0 0 0 0 3.2 3.6 0 3.6 2.8 3.3
SpindlePole -2.5 -2.3 0 0 -2.8 0 0 0 0 -2.7 -2.2 -0.6 1.3 1.6 1.2
Vacuole -1.9 -1.0 -1.9 -2.3 -2.0 -1.8 -2.6 -4.7 -3.3 0.4 -1.2 -1.3 -1.3 0.3 0.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin
Bud
Bud Neck
Bud Periphery
Bud Site
Cell Periphery
Cytoplasm
Cytoplasmic Foci
Eisosomes
Endoplasmic Reticulum
Endosome
Golgi
Lipid Particles
Mitochondria
None
Nuclear Periphery
Nucleolus
Nucleus
Peroxisomes
Punctate Nuclear
Vacuole
Vacuole Periphery

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.9845 10.0395 11.9195 11.6788 9.8286 7.2958 12.1811 11.647 11.8016 16.4195
Translational Efficiency 0.2973 0.2261 0.1879 0.1881 0.2672 0.3331 0.3507 0.2241 0.2546 0.1956

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress
Localization
Cell Percentages mitochondrion (93%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Cbp1

Cbp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cbp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available