Standard name
Human Ortholog
Description Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.87 0.85 0.98 0.94 0.84 0.8 0.71 0.71 0.62 0.64 0.55 0.57 0.97 0.98 0.98 0.13 0.1 0.13 0.74 0.57 0.65 0.83 0.76 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.2 0.05 0 0 0 0.17 0.29 0.23 0.49 0.35 0.47 0.44 0 0 0 0 0 0 0.12 0.19 0.16 0.06 0 0.05
Nucleus 0 0 0 0 0.08 0.09 0.11 0.15 0.11 0.12 0.13 0.12 0 0 0 0.55 0.59 0.5 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Vac/Vac Membrane 0 0.07 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.36 0.32 0.32 0 0.08 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 1 2 3 2 5 3
Bud 1 0 0 0 0 1 11 7 11 5 11 9 0 0 1 0 0 2 0 2 2 1 14 8
Bud Neck 0 0 3 7 8 2 8 6 3 8 5 6 2 1 2 0 0 0 0 1 0 0 4 2
Bud Site 0 0 0 0 0 0 1 1 2 2 2 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 1 2 0 2 0 1 0 1 0 0 1 1 1 0 0 0 0 0 2 1 2 1
Cytoplasm 120 86 238 240 272 276 309 259 192 197 139 145 229 314 374 22 14 15 108 71 152 141 223 208
Endoplasmic Reticulum 2 1 2 0 0 0 0 1 0 0 0 0 0 0 0 12 3 2 1 0 3 0 1 1
Endosome 0 2 0 0 2 1 0 1 0 0 0 0 0 0 2 7 2 5 0 4 6 0 2 1
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 1 0 0 1 0 0 1 0
Mitochondria 27 5 0 5 10 59 127 83 151 109 118 111 0 1 0 1 5 4 18 24 36 10 9 12
Nucleus 0 2 0 11 27 32 49 55 34 37 34 30 2 1 2 94 83 58 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2
Nucleolus 0 0 0 2 0 0 2 3 3 0 3 1 0 1 2 0 0 2 0 0 0 0 2 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 3 1 0 4 5 2 7 1 3 7 1 0 1 0 0 1 5 3 10 2 10 9
Vac/Vac Membrane 0 7 2 0 14 16 17 9 6 8 6 7 2 5 1 61 45 38 2 9 3 3 4 3
Unique Cell Count 138 101 244 256 323 346 435 364 309 310 252 255 235 322 383 170 140 117 146 125 234 170 295 265
Labelled Cell Count 153 104 249 268 334 393 529 428 409 368 321 318 238 325 386 197 154 128 146 125 234 170 295 265


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 2.9 4.8 4.5 4.1 4.6 3.9 3.5 4.0 3.3 3.6 3.2 3.5 5.2 4.8 5.1 9.6 9.0 9.4 4.3 5.2 5.6
Std Deviation (1e-4) 0.3 1.3 1.3 1.2 1.8 1.7 1.4 1.3 1.5 1.9 1.2 1.3 9.8 1.6 1.9 2.3 2.4 5.7 1.2 2.6 2.4
Intensity Change (Log2) – – – -0.15 0.05 -0.22 -0.35 -0.17 -0.44 -0.33 -0.5 -0.34 0.22 0.11 0.17 1.09 0.99 1.06 -0.07 0.2 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 9.8 9.0 9.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.1043 -0.6308 -1.0132 -1.1344 -1.434 -0.6366 -0.2402 -0.4565 -0.5673 -0.3976 -0.8417 -0.439 -1.1257 -0.8208 -0.8153 -0.704 -0.9499 -0.9137
Actin 0.0025 0.0006 0.0003 0.0005 0.0003 0.0035 0.0474 0.0002 0.0115 0.0177 0.0005 0.0087 0.0132 0.0012 0.012 0.0003 0.0415 0.0015
Bud 0.0041 0.0024 0.0004 0.0002 0.0001 0.0036 0.0016 0.0002 0.0015 0.0005 0.0211 0.0004 0.0009 0.0015 0.0029 0 0.0114 0.0005
Bud Neck 0.0008 0.0014 0.0003 0.0003 0.0004 0.0028 0.0043 0.0002 0.001 0.0196 0.0002 0.0014 0.0006 0.0004 0.0015 0.0002 0.0012 0.0039
Bud Periphery 0.0023 0.0007 0.0006 0.0002 0.0001 0.0013 0.0027 0.0002 0.0023 0.0009 0.0053 0.0007 0.0008 0.0013 0.0026 0 0.0026 0.0005
Bud Site 0.0011 0.0144 0.0004 0.0002 0.0001 0.0003 0.0052 0.0017 0.0018 0.002 0.0003 0.0008 0.0012 0.0049 0.0041 0.0001 0.0006 0.0022
Cell Periphery 0.0002 0.0002 0 0 0 0.0001 0.0006 0.0002 0.0002 0.0004 0.0001 0.0001 0.0001 0.0002 0.0003 0 0.0002 0.0001
Cytoplasm 0.1149 0.1282 0.0777 0.0837 0.078 0.1267 0.0979 0.1136 0.1055 0.065 0.0998 0.1649 0.1804 0.2363 0.14 0.2218 0.1554 0.2258
Cytoplasmic Foci 0.0138 0.0092 0.0072 0.0032 0.0096 0.0147 0.0458 0.013 0.0204 0.049 0.0031 0.011 0.0123 0.0184 0.0156 0.004 0.0063 0.014
Eisosomes 0.0002 0.0001 0.0001 0.0002 0.0007 0.0001 0.0009 0.0001 0.0002 0.0001 0 0.0001 0.0002 0.0001 0.0002 0 0.0005 0.0001
Endoplasmic Reticulum 0.0023 0.0021 0.0017 0.0006 0.001 0.0037 0.0028 0.0015 0.0028 0.0033 0.0015 0.0027 0.004 0.0027 0.0034 0.0011 0.0014 0.0023
Endosome 0.0057 0.0033 0.0028 0.0006 0.0024 0.009 0.0203 0.0014 0.0058 0.0219 0.0012 0.0044 0.0068 0.0043 0.0061 0.0009 0.002 0.0052
Golgi 0.0006 0.0002 0.0001 0 0.0001 0.0007 0.0067 0.0001 0.0006 0.0089 0 0.0012 0.0011 0.0002 0.0013 0 0.0008 0.0003
Lipid Particles 0.002 0.0004 0.0005 0.0002 0.0007 0.0042 0.0209 0.0008 0.002 0.0043 0.0001 0.0006 0.002 0.0006 0.0032 0 0.0006 0.0005
Mitochondria 0.0012 0.0007 0.001 0.0007 0.0003 0.0011 0.0037 0.0005 0.0022 0.0041 0.0007 0.0008 0.0016 0.0017 0.0054 0.0006 0.0007 0.0019
None 0.6949 0.6768 0.7486 0.7731 0.7365 0.6461 0.6153 0.8183 0.7594 0.7206 0.7228 0.7283 0.6183 0.5903 0.6149 0.5835 0.6348 0.5663
Nuclear Periphery 0.0063 0.0072 0.0136 0.0027 0.0037 0.0097 0.0052 0.0027 0.0058 0.0054 0.0057 0.0049 0.0116 0.0052 0.0083 0.0029 0.0044 0.0079
Nucleolus 0.0042 0.002 0.0024 0.0011 0.0023 0.0023 0.0029 0.0006 0.0021 0.0022 0.0008 0.0007 0.0024 0.002 0.0046 0.0007 0.0012 0.0017
Nucleus 0.1211 0.1351 0.1218 0.1246 0.1249 0.1306 0.0764 0.0381 0.0607 0.0466 0.1317 0.0576 0.1201 0.1147 0.1427 0.1802 0.1048 0.1339
Peroxisomes 0.0017 0.0003 0.0004 0.0002 0.0002 0.0009 0.0153 0.0006 0.0018 0.014 0.0001 0.0006 0.0028 0.0005 0.0028 0.0001 0.0004 0.0005
Punctate Nuclear 0.0144 0.0124 0.018 0.0069 0.0377 0.0369 0.0209 0.0043 0.008 0.0067 0.0034 0.008 0.0149 0.0101 0.024 0.003 0.0278 0.0287
Vacuole 0.0049 0.002 0.0015 0.0007 0.0007 0.0013 0.0026 0.0014 0.0037 0.0052 0.0012 0.0017 0.0042 0.003 0.0033 0.0005 0.0012 0.0017
Vacuole Periphery 0.0007 0.0003 0.0005 0.0002 0.0002 0.0004 0.0007 0.0002 0.0007 0.0014 0.0003 0.0004 0.0007 0.0004 0.0008 0.0001 0.0003 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.1699 0.9699 6.4773 7.0181 2.8134 7.5905 5.8839 8.2734 8.1191 5.3067
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility
Localization
Cell Percentages cytoplasm (11%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mho1

Mho1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mho1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available