ORF | |
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Human Ortholog | |
Description | Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Bud | 1 | 0 | 0 | 0 | 1 | 4 | 7 | 3 | 6 | 3 | 4 | 4 | 0 | 0 | 0 | 2 | 0 | 0 | 3 | 1 | 1 | 2 | 5 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 3 | 8 | 12 | 4 | 18 | 7 | 6 | 0 | 0 | 0 | 0 | 1 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 2 | 5 | 8 | 13 | 16 | 15 | 21 | 17 | 15 | 8 | 8 | 29 | 88 | 93 | 26 | 38 | 21 | 1 | 1 | 1 | 10 | 17 | 13 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 14 | 8 | 5 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 3 | 0 | 1 | 0 | 3 | 1 | 2 | 3 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 7 | 4 | 2 | 1 | 0 | 0 | 3 | 6 | 6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 4 |
Mitochondria | 121 | 16 | 5 | 12 | 25 | 118 | 247 | 144 | 87 | 250 | 149 | 155 | 2 | 0 | 0 | 4 | 19 | 6 | 5 | 0 | 3 | 4 | 13 | 15 |
Nucleus | 244 | 103 | 176 | 146 | 200 | 315 | 380 | 344 | 162 | 308 | 173 | 200 | 236 | 413 | 363 | 142 | 174 | 110 | 248 | 104 | 173 | 280 | 305 | 326 |
Nuclear Periphery | 4 | 0 | 1 | 0 | 2 | 2 | 3 | 3 | 4 | 3 | 5 | 5 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 5 | 0 | 0 | 0 | 1 | 4 | 18 | 11 | 2 | 5 | 14 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 15 | 22 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 4 | 0 | 1 | 0 | 0 | 0 | 1 | 6 | 1 | 0 | 0 | 1 | 0 | 5 | 1 | 1 | 0 | 2 | 2 | 0 | 2 | 8 | 14 | 21 |
Vac/Vac Membrane | 6 | 7 | 3 | 5 | 4 | 10 | 17 | 8 | 8 | 9 | 10 | 3 | 2 | 11 | 12 | 20 | 36 | 27 | 43 | 7 | 19 | 39 | 72 | 104 |
Unique Cell Count | 284 | 114 | 186 | 159 | 226 | 377 | 507 | 434 | 217 | 407 | 231 | 263 | 269 | 506 | 465 | 199 | 245 | 160 | 315 | 119 | 208 | 358 | 463 | 541 |
Labelled Cell Count | 397 | 128 | 192 | 171 | 249 | 473 | 699 | 555 | 294 | 611 | 370 | 391 | 269 | 518 | 471 | 220 | 282 | 174 | 315 | 119 | 208 | 358 | 463 | 541 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 4.7 | 5.2 | 5.0 | 4.7 | 4.2 | 4.0 | 4.1 | 4.1 | 3.7 | 3.7 | 3.5 | 6.2 | 6.3 | 6.2 | 6.1 | 6.1 | 6.4 | 6.1 | 6.3 | 6.5 |
Std Deviation (1e-4) | 0.5 | 0.7 | 1.4 | 2.5 | 1.3 | 1.6 | 1.3 | 1.6 | 1.6 | 0.8 | 1.3 | 0.9 | 1.0 | 1.1 | 1.1 | 1.2 | 1.5 | 1.4 | 1.1 | 1.2 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.14 | -0.29 | -0.37 | -0.35 | -0.32 | -0.48 | -0.48 | -0.55 | 0.27 | 0.28 | 0.27 | 0.25 | 0.24 | 0.3 | 0.24 | 0.28 | 0.34 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.5 | 4.7 | 5.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2576 | 1.845 | 1.4236 | 1.6821 | 1.0996 | 1.5933 | 0.5389 | 0.1659 | 0.1947 | -0.0016 | 0.6598 | 0.2625 | 0.2725 | 0.5557 | 0.2044 | 0.0649 | -0.4513 | 0.1807 |
Actin | 0.0002 | 0.0001 | 0.0029 | 0.0001 | 0.0001 | 0.0001 | 0.065 | 0.0001 | 0.0009 | 0.0019 | 0.0009 | 0.0083 | 0.0055 | 0 | 0.0065 | 0.0153 | 0.0001 | 0.0099 |
Bud | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0005 | 0.0008 | 0.0002 | 0.0006 | 0.0005 | 0 | 0.0012 | 0.0166 | 0.0001 | 0.0046 |
Bud Neck | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0032 | 0.0028 | 0.0084 | 0.0003 | 0.0028 | 0.0015 | 0.0014 | 0.0028 | 0.0017 | 0.0001 | 0.0008 | 0.0003 | 0.0008 | 0.0017 |
Bud Periphery | 0 | 0 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.0015 | 0.0001 | 0.0013 | 0.0032 | 0.0002 | 0.0009 | 0.0006 | 0 | 0.001 | 0.0047 | 0 | 0.0013 |
Bud Site | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0048 | 0.0004 | 0.0054 | 0.0017 | 0.0008 | 0.0007 | 0.0024 | 0.0002 | 0.0084 | 0.0026 | 0.0001 | 0.0007 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0005 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0002 | 0 | 0.0001 |
Cytoplasm | 0.0208 | 0.0056 | 0.0094 | 0.0032 | 0.0088 | 0.0107 | 0.0469 | 0.0225 | 0.0123 | 0.0038 | 0.021 | 0.012 | 0.037 | 0.0097 | 0.0201 | 0.0042 | 0.0091 | 0.0087 |
Cytoplasmic Foci | 0.0004 | 0.0001 | 0.0008 | 0 | 0.0007 | 0.0051 | 0.0252 | 0.0004 | 0.0059 | 0.0016 | 0.0038 | 0.0063 | 0.0309 | 0.01 | 0.0039 | 0.0024 | 0.0028 | 0.0062 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0011 | 0.0002 | 0.001 | 0.0001 | 0.0002 | 0.0004 | 0.0064 | 0.0021 | 0.0013 | 0.0006 | 0.0014 | 0.0012 | 0.0031 | 0.0003 | 0.0026 | 0.0004 | 0.0002 | 0.0018 |
Endosome | 0.0007 | 0.0002 | 0.0026 | 0.0001 | 0.0003 | 0.0004 | 0.0334 | 0.001 | 0.0032 | 0.0163 | 0.0421 | 0.0092 | 0.0281 | 0.0011 | 0.0097 | 0.0058 | 0.0005 | 0.0075 |
Golgi | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0.0125 | 0 | 0.0011 | 0.0305 | 0.0322 | 0.0052 | 0.0081 | 0.0001 | 0.0015 | 0.0052 | 0 | 0.004 |
Lipid Particles | 0.0001 | 0 | 0.0008 | 0 | 0.0001 | 0.0001 | 0.0334 | 0.0001 | 0.0024 | 0.0006 | 0.0066 | 0.01 | 0.0169 | 0.0004 | 0.0015 | 0.0037 | 0.0001 | 0.0061 |
Mitochondria | 0.0005 | 0.0002 | 0.0043 | 0.0003 | 0.0004 | 0.0012 | 0.0166 | 0.0004 | 0.0013 | 0.0089 | 0.0062 | 0.018 | 0.005 | 0.0003 | 0.0016 | 0.0284 | 0.0002 | 0.0032 |
None | 0.0937 | 0.0349 | 0.0332 | 0.0002 | 0.0422 | 0.0338 | 0.0172 | 0.0081 | 0.0013 | 0.0006 | 0.042 | 0.0148 | 0.0329 | 0.0221 | 0.0226 | 0.0035 | 0.0019 | 0.0027 |
Nuclear Periphery | 0.0133 | 0.0239 | 0.0202 | 0.0093 | 0.0066 | 0.0031 | 0.0235 | 0.0192 | 0.0146 | 0.0382 | 0.0115 | 0.0087 | 0.028 | 0.011 | 0.0153 | 0.0033 | 0.007 | 0.0116 |
Nucleolus | 0.0061 | 0.0065 | 0.0057 | 0.0028 | 0.0103 | 0.0155 | 0.0093 | 0.0073 | 0.0063 | 0.0143 | 0.0163 | 0.0158 | 0.0182 | 0.0036 | 0.0042 | 0.0061 | 0.0111 | 0.0113 |
Nucleus | 0.8431 | 0.9078 | 0.8955 | 0.9816 | 0.9155 | 0.898 | 0.6327 | 0.926 | 0.9217 | 0.8674 | 0.7935 | 0.8461 | 0.7294 | 0.9378 | 0.8759 | 0.8538 | 0.8764 | 0.9014 |
Peroxisomes | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0254 | 0 | 0.0021 | 0.0003 | 0.0004 | 0.0062 | 0.0172 | 0 | 0.0069 | 0.0028 | 0 | 0.0013 |
Punctate Nuclear | 0.0182 | 0.0193 | 0.0209 | 0.0017 | 0.0089 | 0.028 | 0.0212 | 0.0104 | 0.0109 | 0.0037 | 0.0162 | 0.0288 | 0.0296 | 0.0027 | 0.013 | 0.0387 | 0.0892 | 0.014 |
Vacuole | 0.0009 | 0.0003 | 0.0006 | 0.0001 | 0.0015 | 0.0004 | 0.0097 | 0.0011 | 0.0037 | 0.0023 | 0.002 | 0.0031 | 0.0027 | 0.0004 | 0.0022 | 0.0011 | 0.0002 | 0.0009 |
Vacuole Periphery | 0.0002 | 0.0003 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0052 | 0.0003 | 0.0009 | 0.0014 | 0.0014 | 0.001 | 0.0018 | 0.0002 | 0.0009 | 0.0007 | 0.0001 | 0.0007 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.7107 | 13.9582 | 9.7821 | 11.4562 | 14.0587 | 11.2707 | 13.133 | 10.0546 | 11.0493 | 11.3451 |
Translational Efficiency | 0.95 | 0.6111 | 0.8145 | 0.7616 | 0.6032 | 0.6652 | 0.7555 | 0.7983 | 0.7849 | 0.7676 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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