Standard name
Human Ortholog
Description Peroxisomal matrix protein with naturally active promoter; well-conserved in fungi; localized to peroxisomes under physiological growth conditions; levels of some amino acids are altered upon both knockout and overexpression, suggesting potential involvement of Pxp2p in amino acid metabolism or related cellular metabolic processes (needs further study); GFP-fusion protein displays inherent dual localization with large proportion localizing to cytosol
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0 0.06 0 0 0.05 0.05
Bud 0.05 0.05 0.09 0.06 0 0 0 0 0.05 0 0 0.05 0 0 0 0.11 0.09 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.09 0 0 0.05 0 0 0.05 0 0 0 0 0.05 0.07 0.15 0.17 0.11 0.17 0 0 0 0 0.06 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0 0
Endosome 0.19 0 0.15 0.12 0.2 0.13 0.15 0.11 0 0 0 0 0.08 0.21 0.16 0.27 0.17 0.18 0.2 0.13 0.13 0.14 0.11 0.11
Golgi 0.05 0 0.07 0.07 0.05 0 0 0 0 0 0 0 0.18 0.16 0.09 0.05 0.27 0.24 0.08 0.07 0.05 0.1 0.07 0.08
Mitochondria 0.69 0.86 0.41 0.73 0.6 0.76 0.73 0.71 0.93 0.88 0.93 0.9 0.7 0.49 0.47 0.1 0.06 0.09 0.35 0.48 0.43 0.43 0.43 0.45
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Peroxisomes 0.12 0.06 0.33 0.18 0.12 0.05 0.06 0.05 0 0 0 0 0.07 0.12 0.12 0.35 0.17 0.1 0.23 0.14 0.3 0.15 0.18 0.14
SpindlePole 0.08 0 0.26 0.06 0.1 0.07 0.11 0.1 0.05 0.08 0.05 0.07 0.08 0.13 0.07 0.15 0.15 0.18 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.1 0.06 0 0.14 0.12 0.13 0.11 0.05 0.05 0.05 0 0.06 0.09 0.08 0.15 0.14 0.16 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 1 21 11 4 4 1 1 0 0 2 1 6 7 2 3 10 16 7 8 15 3 13 15
Bud 9 6 39 25 16 22 31 24 43 37 33 44 3 0 3 18 13 10 2 0 5 0 2 3
Bud Neck 0 0 0 0 1 0 0 0 0 2 0 1 0 0 0 0 0 0 0 1 0 0 2 0
Bud Site 4 2 8 2 1 3 5 2 14 7 8 13 0 0 0 4 1 5 – – – – – –
Cell Periphery 4 3 7 13 8 5 5 4 5 12 10 15 3 3 2 5 6 7 0 0 0 0 1 0
Cytoplasm 2 11 18 16 24 19 20 35 25 18 11 12 7 11 32 29 16 30 0 4 2 6 15 21
Endoplasmic Reticulum 5 3 1 4 3 2 3 0 4 3 3 5 3 5 12 7 9 4 0 0 1 0 0 3
Endosome 36 3 67 49 93 79 109 80 6 18 18 23 12 33 35 46 24 31 43 18 69 20 28 32
Golgi 10 3 33 30 22 4 3 4 1 0 3 0 26 25 20 8 39 42 17 10 28 14 19 25
Mitochondria 134 108 184 310 282 474 543 513 876 770 730 875 100 78 103 17 9 15 76 69 234 61 118 132
Nucleus 1 1 0 1 2 14 15 16 17 17 16 15 2 1 1 1 1 2 0 1 0 1 2 1
Nuclear Periphery 1 0 0 2 3 11 13 13 19 28 12 20 1 0 0 0 0 0 0 0 1 1 1 2
Nucleolus 3 0 2 4 5 13 14 26 21 16 13 15 2 0 1 3 0 9 0 0 0 0 0 2
Peroxisomes 24 7 150 76 57 31 42 37 16 25 7 20 10 19 26 59 24 17 49 19 164 22 48 41
SpindlePole 15 1 116 25 46 44 81 73 47 70 41 67 12 20 15 25 22 31 7 1 13 3 10 5
Vac/Vac Membrane 23 13 29 16 67 73 97 80 46 46 41 32 8 15 18 26 20 28 4 6 2 4 2 4
Unique Cell Count 193 126 454 423 470 625 740 718 941 872 784 970 142 158 217 169 142 175 215 146 551 144 272 297
Labelled Cell Count 274 162 675 584 634 798 982 908 1140 1069 948 1158 195 217 270 251 194 247 215 146 551 144 272 297


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 5.1 6.8 6.6 6.9 5.8 5.5 5.7 4.3 4.8 4.7 4.7 6.5 6.5 6.1 6.7 8.5 8.5 6.9 7.4 7.4
Std Deviation (1e-4) 0.8 1.0 1.3 1.5 1.6 1.3 1.3 1.3 1.0 1.1 0.9 0.9 1.4 1.4 1.6 1.5 1.7 1.5 1.7 2.0 1.9
Intensity Change (Log2) – – – -0.05 0.01 -0.25 -0.32 -0.27 -0.66 -0.5 -0.53 -0.53 -0.06 -0.08 -0.15 -0.03 0.31 0.32 0.01 0.12 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.6 -3.5 -4.3 -5.6 -5.5 0 0 0 0 -0.2 -0.1 -2.5 -1.6 1.1 2.2
Bud -1.5 -3.3 -3.6 -3.1 -3.9 0 0 0 0 -2.6 -3.8 -3.6 0.8 0.2 -1.2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.5 0.2 -1.1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.1 0.8 -0.8 -1.2 0.7 -1.3 -2.0 -2.9 -3.3 0.5 1.5 5.0 5.5 3.3 5.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -1.4 2.0 -1.0 0 -1.8 0 0 0 0 -1.9 1.8 0.5 3.6 0.6 0.9
Golgi -0.1 -1.7 -5.9 -6.7 -6.4 0 0 0 0 3.8 3.2 0.9 -1.1 6.4 5.8
Mitochondria 9.8 5.9 11.7 11.3 10.5 0 0 0 0 6.2 1.9 1.7 -7.2 -7.6 -7.7
Nucleus 0 0 3.2 3.1 3.2 2.9 3.0 3.1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 2.9 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 2.3 2.1 3.5 0 0 0 0 0 0 0 0 0 0
Peroxisomes -5.1 -7.6 -12.2 -12.5 -12.7 0 0 0 0 -6.1 -5.1 -5.8 0.4 -3.7 -5.9
SpindlePole -7.9 -6.3 -8.4 -6.6 -7.0 0 0 0 0 -4.3 -3.4 -5.7 -2.9 -2.5 -2.1
Vacuole -1.7 3.9 2.9 3.7 2.7 -1.2 -0.8 -0.9 -2.7 -0.3 1.3 0.9 3.5 2.9 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.3195 8.043 7.3109 6.1239 6.2226 7.7151 7.6608 9.8855 8.8 8.5183 8.7818 9.4067 5.6921 6.2359 6.8434 5.3323 5.8363 6.6298
Actin 0.0489 0.0556 0.0416 0.0407 0.0034 0.0093 0.1158 0.0188 0.0711 0.0137 0.0533 0.0509 0.0741 0.047 0.031 0.0509 0.0468 0.0316
Bud 0.0004 0.0035 0.0003 0.0006 0.0002 0.0001 0.0007 0.0004 0.0015 0.0001 0.0004 0.0002 0.0004 0.0015 0.0004 0.0155 0.0005 0.0044
Bud Neck 0.0006 0.0004 0.0004 0.0002 0.0001 0.0002 0.0008 0.0004 0.001 0.0001 0.0006 0.0003 0.0005 0.0005 0.0007 0.0006 0.0008 0.0011
Bud Periphery 0.0007 0.0021 0.0005 0.0011 0.0003 0.0002 0.0011 0.0005 0.0028 0.0002 0.001 0.0006 0.0007 0.0017 0.0005 0.0213 0.0009 0.0031
Bud Site 0.0015 0.0092 0.0017 0.0013 0.0003 0.0002 0.0032 0.0023 0.0064 0.0002 0.0046 0.0004 0.0024 0.0196 0.0025 0.0049 0.0027 0.0013
Cell Periphery 0.0003 0.0002 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0 0.0007 0.0002 0.0003 0.0004 0.0002 0.0004 0.0002 0.0002
Cytoplasm 0.0002 0.0002 0.0002 0.0001 0.0002 0.0001 0.0006 0.0052 0.0006 0 0.0015 0.0001 0.0007 0.0006 0.0003 0.0053 0.0003 0.0003
Cytoplasmic Foci 0.0158 0.0172 0.0122 0.0119 0.0127 0.0045 0.0294 0.0265 0.0149 0.0167 0.0149 0.008 0.0265 0.0207 0.0133 0.0214 0.0132 0.0102
Eisosomes 0.0006 0.0004 0.0006 0.0005 0.0002 0.0003 0.0009 0.0008 0.0008 0.0003 0.0016 0.0007 0.0005 0.0004 0.0003 0.0003 0.0006 0.0007
Endoplasmic Reticulum 0.0002 0.0001 0.0002 0.0001 0.0002 0.0002 0.0028 0.0008 0.0002 0 0.0025 0.0001 0.0003 0.0003 0.0002 0.0008 0.0006 0.0002
Endosome 0.0248 0.0257 0.0269 0.0051 0.046 0.0125 0.0298 0.0362 0.012 0.0043 0.0122 0.0149 0.0363 0.0428 0.0211 0.0154 0.0317 0.0191
Golgi 0.0762 0.0843 0.064 0.0146 0.0423 0.0633 0.1 0.0691 0.0553 0.013 0.0662 0.0887 0.0994 0.1209 0.075 0.0312 0.0966 0.0859
Lipid Particles 0.0455 0.0132 0.0298 0.0181 0.0277 0.0117 0.0536 0.0059 0.0371 0.0304 0.016 0.0123 0.0595 0.0101 0.0247 0.0239 0.0129 0.0386
Mitochondria 0.669 0.6849 0.7143 0.7472 0.7767 0.8174 0.545 0.7275 0.7288 0.8324 0.7203 0.7276 0.6073 0.6365 0.7716 0.7086 0.6964 0.7102
None 0.0134 0.0001 0.0001 0.0002 0.0001 0.0001 0.0006 0.0001 0.0002 0 0.0061 0.0001 0.0018 0.0001 0.0001 0.0027 0.0003 0.0001
Nuclear Periphery 0.0002 0.0001 0.0001 0.0002 0.0003 0.0001 0.0068 0.0003 0.0001 0 0.001 0 0.0007 0.0002 0.0001 0.0027 0.0005 0.0005
Nucleolus 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0014 0.0001 0.0001 0 0.0005 0.0001 0.0054 0.0002 0.0003 0.0003 0.0001 0.0002
Nucleus 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0008 0.0001 0.0001 0 0.0005 0.0001 0.0009 0.0002 0.0001 0.0016 0.0002 0.0002
Peroxisomes 0.096 0.1001 0.1019 0.1563 0.0823 0.0762 0.0894 0.1021 0.064 0.0876 0.0936 0.091 0.068 0.089 0.0541 0.0753 0.0907 0.0889
Punctate Nuclear 0.0003 0.0001 0.0001 0.0001 0.0001 0 0.0017 0.0002 0.0002 0.0004 0.001 0 0.0022 0.0001 0.0003 0.0152 0.0004 0.0003
Vacuole 0.0022 0.001 0.0017 0.0004 0.003 0.0011 0.0094 0.001 0.0009 0.0001 0.0007 0.0019 0.0059 0.0034 0.0015 0.0012 0.0012 0.0008
Vacuole Periphery 0.0028 0.0013 0.0032 0.001 0.0033 0.0024 0.0059 0.0017 0.0019 0.0002 0.0007 0.0018 0.006 0.0038 0.0018 0.0005 0.0026 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.2458 19.6812 9.4498 15.8238 10.9143 10.3549 16.388 15.8011 15.2589 18.7968
Translational Efficiency 1.2376 0.9744 1.5344 0.8658 1.5903 1.4586 1.071 0.7982 0.9027 0.9159

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Peroxisomal matrix protein with naturally active promoter; well-conserved in fungi; localized to peroxisomes under physiological growth conditions; levels of some amino acids are altered upon both knockout and overexpression, suggesting potential involvement of Pxp2p in amino acid metabolism or related cellular metabolic processes (needs further study); GFP-fusion protein displays inherent dual localization with large proportion localizing to cytosol
Localization
Cell Percentages mitochondrion (85%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Pxp2

Pxp2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pxp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available