Standard name | |
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Human Ortholog | |
Description | Mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S7 ribosomal protein |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 4 | 1 | 4 | 0 | 3 | 0 | 2 | 1 | 0 | 1 | 0 | 2 | 1 | – | – | – | 1 | 0 | 0 | 0 | 1 | 4 |
Bud | 1 | 1 | 2 | 5 | 2 | 3 | 3 | 3 | 5 | 9 | 6 | 12 | 0 | 1 | 0 | – | – | – | 0 | 0 | 0 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | 1 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 5 | 1 | 4 | 3 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 1 | 5 | 7 | 3 | 3 | 3 | 1 | 5 | 2 | 2 | 0 | 4 | 2 | 4 | 6 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 3 | 1 | 4 | 5 | 7 | 8 | 14 | 3 | 7 | 6 | 2 | 6 | 5 | 5 | – | – | – | 0 | 0 | 0 | 0 | 1 | 1 |
Endoplasmic Reticulum | 1 | 1 | 0 | 0 | 1 | 3 | 2 | 0 | 0 | 1 | 2 | 1 | 1 | 0 | 2 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 7 | 4 | 11 | 5 | 16 | 19 | 38 | 20 | 1 | 7 | 10 | 15 | 4 | 8 | 9 | – | – | – | 7 | 7 | 7 | 3 | 9 | 10 |
Golgi | 6 | 0 | 32 | 28 | 54 | 10 | 5 | 13 | 0 | 4 | 1 | 3 | 15 | 28 | 31 | – | – | – | 7 | 6 | 8 | 5 | 14 | 13 |
Mitochondria | 211 | 169 | 160 | 186 | 136 | 250 | 291 | 241 | 362 | 336 | 321 | 371 | 85 | 117 | 121 | – | – | – | 197 | 157 | 203 | 62 | 135 | 159 |
Nucleus | 0 | 0 | 2 | 1 | 2 | 5 | 2 | 6 | 2 | 1 | 4 | 4 | 1 | 1 | 0 | – | – | – | 0 | 0 | 1 | 0 | 1 | 0 |
Nuclear Periphery | 14 | 4 | 11 | 9 | 14 | 34 | 37 | 37 | 53 | 52 | 42 | 50 | 13 | 14 | 8 | – | – | – | 2 | 0 | 2 | 1 | 2 | 5 |
Nucleolus | 16 | 5 | 11 | 3 | 12 | 38 | 53 | 36 | 54 | 34 | 50 | 39 | 10 | 3 | 3 | – | – | – | 1 | 0 | 0 | 0 | 2 | 3 |
Peroxisomes | 7 | 2 | 17 | 5 | 3 | 4 | 9 | 5 | 2 | 6 | 3 | 10 | 0 | 0 | 6 | – | – | – | 10 | 3 | 19 | 0 | 8 | 17 |
SpindlePole | 1 | 1 | 7 | 2 | 7 | 6 | 11 | 4 | 14 | 10 | 6 | 15 | 3 | 6 | 0 | – | – | – | 0 | 1 | 2 | 0 | 1 | 2 |
Vac/Vac Membrane | 30 | 33 | 26 | 18 | 36 | 55 | 23 | 31 | 16 | 17 | 8 | 14 | 7 | 15 | 37 | – | – | – | 2 | 7 | 2 | 2 | 11 | 5 |
Unique Cell Count | 225 | 181 | 211 | 211 | 208 | 346 | 384 | 341 | 405 | 391 | 373 | 433 | 112 | 142 | 165 | 237 | 186 | 251 | 78 | 198 | 232 | |||
Labelled Cell Count | 297 | 228 | 291 | 271 | 295 | 438 | 486 | 416 | 521 | 489 | 463 | 544 | 147 | 204 | 229 | 237 | 186 | 251 | 78 | 198 | 232 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.0 | 6.5 | 8.6 | 8.5 | 9.6 | 8.1 | 7.8 | 8.3 | 6.7 | 7.3 | 6.9 | 7.5 | 7.8 | 8.0 | 8.3 | – | – | – | 9.1 | 10.4 | 9.8 |
Std Deviation (1e-4) | 1.6 | 1.1 | 1.5 | 1.4 | 1.9 | 1.6 | 1.6 | 1.5 | 1.5 | 1.7 | 1.6 | 1.7 | 1.9 | 1.5 | 1.6 | – | – | – | 2.0 | 2.7 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.16 | -0.09 | -0.15 | -0.06 | -0.37 | -0.24 | -0.33 | -0.2 | -0.15 | -0.1 | -0.06 | – | – | – | 0.08 | 0.27 | 0.19 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | -1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.2 | – | – | – |
Cytoplasm | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Endosome | -1.5 | 1.0 | 0.1 | 2.0 | 0.3 | 0 | -2.4 | -1.6 | -1.1 | -0.7 | 0.2 | 0.1 | – | – | – |
Golgi | -0.6 | 2.7 | -5.3 | -6.7 | -4.7 | -8.0 | -7.0 | -7.5 | -7.6 | -0.4 | 1.1 | 0.9 | – | – | – |
Mitochondria | 3.3 | -2.3 | -0.9 | 0 | -1.3 | 4.4 | 3.1 | 3.1 | 3.1 | 0 | 1.5 | -0.6 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | -0.5 | 0.7 | 1.9 | 1.9 | 2.3 | 3.0 | 3.1 | 2.4 | 2.6 | 2.1 | 1.7 | -0.2 | – | – | – |
Nucleolus | -2.2 | 0.2 | 2.3 | 3.2 | 2.2 | 3.1 | 1.6 | 3.1 | 1.7 | 1.3 | -1.5 | -1.7 | – | – | – |
Peroxisomes | -2.6 | -3.2 | -4.1 | -3.3 | -3.8 | -5.2 | -4.0 | -4.6 | -3.4 | -3.1 | -3.5 | -1.8 | – | – | – |
SpindlePole | 0 | 0 | 0 | -0.3 | 0 | 0.1 | -0.5 | 0 | 0.1 | 0 | 0.4 | 0 | – | – | – |
Vacuole | -1.3 | 1.4 | 1.2 | -2.7 | -1.2 | -3.9 | -3.6 | -5.0 | -4.5 | -1.7 | -0.5 | 2.6 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.5876 | 6.375 | 6.6714 | 6.375 | 6.8287 | 6.5182 | 9.2851 | 10.2189 | 9.8022 | 9.6986 | 8.7654 | 9.9833 | 7.4875 | 8.1554 | 7.5831 | 7.6206 | 7.3607 | 7.4055 |
Actin | 0.0231 | 0.0024 | 0.0055 | 0.0018 | 0.0044 | 0.0005 | 0.0104 | 0.0035 | 0.012 | 0.002 | 0.0281 | 0.0005 | 0.0087 | 0.0015 | 0.0048 | 0.0265 | 0.0104 | 0.0097 |
Bud | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0006 | 0 | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0004 | 0 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0001 |
Bud Neck | 0.0002 | 0.0001 | 0 | 0 | 0.0004 | 0 | 0.0005 | 0.0001 | 0.0005 | 0 | 0.0002 | 0 | 0.0005 | 0 | 0.0001 | 0.0008 | 0.0002 | 0.0001 |
Bud Periphery | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0012 | 0.0001 | 0.0007 | 0.0003 | 0.0005 | 0.0001 | 0.0006 | 0 | 0.001 | 0.0001 | 0.0008 | 0.0007 | 0.0006 | 0.0001 |
Bud Site | 0.0007 | 0.0006 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0024 | 0.0003 | 0.0052 | 0 | 0.0015 | 0 | 0.0009 | 0.0001 | 0.0004 | 0.0003 | 0.0035 | 0.0002 |
Cell Periphery | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 |
Cytoplasm | 0.0032 | 0.0001 | 0 | 0 | 0.004 | 0 | 0.0074 | 0 | 0.0021 | 0 | 0.0004 | 0 | 0.0033 | 0 | 0.0039 | 0 | 0.0001 | 0.0001 |
Cytoplasmic Foci | 0.0123 | 0.0023 | 0.002 | 0.0007 | 0.0299 | 0.0004 | 0.02 | 0.0029 | 0.0049 | 0.0003 | 0.0041 | 0.0001 | 0.0111 | 0.0014 | 0.0072 | 0.0013 | 0.0042 | 0.0033 |
Eisosomes | 0.0007 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0004 | 0.0006 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.0012 | 0.0001 | 0 | 0 | 0.0019 | 0 | 0.0021 | 0 | 0.0007 | 0 | 0.0002 | 0 | 0.0019 | 0 | 0.0017 | 0.0001 | 0.0002 | 0.0002 |
Endosome | 0.0148 | 0.0148 | 0.0038 | 0.0016 | 0.0184 | 0.0012 | 0.0257 | 0.0037 | 0.0105 | 0.0012 | 0.0048 | 0.0018 | 0.0108 | 0.0066 | 0.01 | 0.0024 | 0.004 | 0.0056 |
Golgi | 0.0145 | 0.0317 | 0.0178 | 0.0043 | 0.0612 | 0.0046 | 0.029 | 0.0086 | 0.0165 | 0.0028 | 0.0085 | 0.006 | 0.0103 | 0.0399 | 0.0117 | 0.0091 | 0.0153 | 0.0103 |
Lipid Particles | 0.0524 | 0.0193 | 0.0037 | 0.0008 | 0.0225 | 0.0033 | 0.0348 | 0.0333 | 0.0063 | 0.0018 | 0.0063 | 0.0121 | 0.0217 | 0.0018 | 0.0088 | 0.0026 | 0.0081 | 0.0047 |
Mitochondria | 0.8244 | 0.904 | 0.9555 | 0.985 | 0.7502 | 0.9826 | 0.8281 | 0.929 | 0.9188 | 0.9831 | 0.8908 | 0.9675 | 0.8896 | 0.9414 | 0.9323 | 0.9529 | 0.9392 | 0.9498 |
None | 0.0082 | 0 | 0 | 0 | 0.0017 | 0 | 0.0033 | 0 | 0.0067 | 0 | 0.0001 | 0 | 0.0011 | 0 | 0.0007 | 0.0001 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0093 | 0.0001 | 0 | 0 | 0.0185 | 0 | 0.0024 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0017 | 0 | 0.001 | 0 | 0.0002 | 0.0002 |
Nucleolus | 0.0096 | 0.0002 | 0 | 0 | 0.0014 | 0.0001 | 0.0011 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0007 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 |
Nucleus | 0.0026 | 0.0001 | 0 | 0 | 0.0344 | 0 | 0.0007 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 |
Peroxisomes | 0.0127 | 0.0111 | 0.0094 | 0.0041 | 0.0048 | 0.0031 | 0.0134 | 0.0154 | 0.0087 | 0.0029 | 0.052 | 0.0012 | 0.0231 | 0.004 | 0.0081 | 0.0013 | 0.0056 | 0.0072 |
Punctate Nuclear | 0.0044 | 0 | 0 | 0 | 0.0158 | 0 | 0.0053 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0.0019 | 0 | 0.0008 | 0 | 0.0005 | 0.0017 |
Vacuole | 0.0014 | 0.0033 | 0.0003 | 0.0003 | 0.0163 | 0.0004 | 0.0036 | 0.0005 | 0.0014 | 0.0006 | 0.0004 | 0.0005 | 0.0066 | 0.0006 | 0.0014 | 0.0002 | 0.003 | 0.0017 |
Vacuole Periphery | 0.0032 | 0.009 | 0.0013 | 0.0006 | 0.0117 | 0.0035 | 0.0081 | 0.0018 | 0.0018 | 0.0046 | 0.0011 | 0.0093 | 0.0035 | 0.0019 | 0.0051 | 0.0006 | 0.0037 | 0.0045 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 44.4921 | 57.0083 | 39.3513 | 42.0375 | 47.1102 | 42.9852 | 53.9547 | 44.7846 | 63.756 | 49.2008 |
Translational Efficiency | 0.8381 | 0.7576 | 0.9847 | 1.1964 | 0.8447 | 1.2013 | 1.0026 | 1.7749 | 1.1966 | 0.9793 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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