Standard name
Human Ortholog
Description Alpha-1,2-mannosidase; involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0.09 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0.06 0 0 0 0 – 0 0 0 0.05 0.1 0.11 – 0.06 0.16 0 0 0 0 0 0
Cytoplasm 0.32 0.39 0.42 0.42 0.52 0.51 0.48 0.48 – 0.44 0.41 0.45 0.32 0.29 0.44 – 0.71 0.55 0.07 0.14 0.05 0 0 0.08
Endoplasmic Reticulum 0.57 0.5 0.33 0.19 0.13 0.11 0.06 0.05 – 0.05 0 0.11 0.66 0.64 0.53 – 0 0 0.75 0.68 0.77 0.72 0.73 0.7
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.11 0.19 0.38 0.31 0.46 0.46 0.51 – 0.63 0.67 0.57 0 0 0 – 0.13 0.16 0.07 0.07 0.08 0.12 0.1 0.08
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.07 0.15 0.1 0.19 0.08 0.15 0.11 – 0.09 0.09 0.05 0 0 0 – 0.09 0.15 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 4 7 4 0 0 0 0 – 0 1 5 4 4 1 – 4 9 1 3 14 10 14 12
Bud 0 3 1 4 2 6 5 13 – 11 20 14 0 0 1 – 0 2 0 1 2 2 3 7
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 2 0 0 2 2 3 3 8
Bud Site 0 0 0 0 0 0 0 1 – 3 2 2 0 0 0 – 0 0 – – – – – –
Cell Periphery 3 4 12 13 11 9 16 9 – 19 14 23 10 18 26 – 10 16 2 2 6 9 12 24
Cytoplasm 44 66 128 89 141 196 218 300 – 246 189 262 66 53 107 – 112 56 13 26 18 13 19 42
Endoplasmic Reticulum 77 84 100 41 35 44 27 33 – 27 15 66 135 115 127 – 3 3 144 134 309 263 340 390
Endosome 0 1 11 6 7 5 7 4 – 0 1 2 1 0 0 – 2 0 1 0 1 1 2 2
Golgi 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 1 0 0 2 1 1
Mitochondria 11 18 57 81 84 179 208 320 – 354 306 331 0 0 0 – 20 16 12 13 31 43 46 41
Nucleus 2 1 0 0 1 2 2 10 – 2 4 9 1 5 2 – 5 2 0 0 0 0 0 0
Nuclear Periphery 4 1 2 1 3 2 5 4 – 6 7 11 0 0 0 – 1 0 5 0 3 0 1 0
Nucleolus 0 0 0 1 0 1 0 1 – 0 1 0 0 0 0 – 0 1 0 0 0 0 0 0
Peroxisomes 0 0 1 0 2 1 0 0 – 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 1 1 2 – 2 1 0 0 0 1 – 3 3 0 0 0 0 0 0
Vac/Vac Membrane 17 11 45 21 51 31 68 71 – 50 42 32 5 4 6 – 15 15 6 9 7 9 16 21
Unique Cell Count 136 168 305 213 269 386 451 630 562 460 583 205 180 241 158 102 191 197 402 365 465 557
Labelled Cell Count 160 193 364 262 337 477 557 768 721 603 757 222 199 271 177 123 191 197 402 365 465 557


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 6.1 5.2 5.5 4.9 4.3 4.4 4.4 – 3.7 3.8 3.8 7.2 7.2 7.7 4.7 5.6 6.8 7.2 7.7 7.8
Std Deviation (1e-4) 1.0 1.1 1.3 1.6 1.4 1.2 1.7 1.1 – 0.9 0.8 0.7 1.1 1.0 1.1 0.9 1.0 1.2 1.5 1.5 1.6
Intensity Change (Log2) – – – 0.06 -0.09 -0.28 -0.24 -0.24 – -0.49 -0.47 -0.45 0.46 0.46 0.55 -0.17 0.11 0.37 0.46 0.56 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Cell Periphery 1.1 0.1 0 0 0 – 0 0 0 0.5 2.7 3.1 – 1.1 4.1
Cytoplasm 0 2.5 2.3 1.7 1.6 – 0.5 -0.2 0.8 -2.2 -2.8 0.6 – 5.9 2.3
Endoplasmic Reticulum -3.4 -5.6 0 0 0 – 0 0 0 7.3 6.7 4.7 – -7.6 -6.0
Endosome -0.5 -0.7 0 0 0 – 0 0 0 0 0 0 – 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Mitochondria 4.9 3.5 0 0 0 – 0 0 0 -6.6 -6.2 -7.1 – -1.7 -0.7
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Vacuole -1.6 1.3 -2.8 0.1 -1.5 – -2.6 -2.4 -4.7 -4.6 -4.4 -4.9 – -1.6 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.3921 3.4908 3.5288 2.849 3.0547 3.4682 2.1085 2.9246 2.6067 2.6575 2.2794 2.7262 3.5284 3.1804 2.8 2.7011 3.5754 2.7912
Actin 0.0105 0.002 0.0093 0.006 0.0184 0.0018 0.008 0.0011 0.0029 0.003 0.0014 0.0005 0.0434 0.0012 0.009 0.0012 0.0001 0.0006
Bud 0.0026 0.009 0.0037 0.0027 0.0331 0.0029 0 0.0002 0.0001 0.0001 0 0 0.0004 0.0001 0.0034 0 0 0
Bud Neck 0.0008 0.001 0.0055 0.0037 0.0028 0.0023 0.0004 0.0003 0.0009 0.0007 0.001 0.0006 0.0024 0.0002 0.0004 0.0003 0 0.0008
Bud Periphery 0.0021 0.0032 0.0021 0.0025 0.0084 0.0018 0.0001 0.0002 0.0003 0.0001 0.0001 0 0.0008 0.0001 0.0043 0.0001 0 0
Bud Site 0.0025 0.0387 0.0136 0.0023 0.0213 0.001 0.0014 0.0037 0.0079 0.0004 0 0 0.0017 0.0007 0.0031 0.0002 0 0
Cell Periphery 0.0019 0.002 0.0025 0.0011 0.0014 0.0008 0.0046 0.0024 0.0037 0.0011 0.0012 0.0005 0.0045 0.0058 0.003 0.0021 0.0002 0.0008
Cytoplasm 0.223 0.3176 0.1992 0.2593 0.1755 0.3209 0.162 0.2312 0.1685 0.1608 0.231 0.1217 0.0924 0.2507 0.225 0.1337 0.0056 0.1861
Cytoplasmic Foci 0.0102 0.0113 0.0176 0.0112 0.0127 0.0175 0.0133 0.0066 0.0097 0.0312 0.0071 0.0045 0.0026 0.0075 0.0076 0.0039 0 0.0021
Eisosomes 0.0002 0.0001 0.0002 0.0002 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0009 0.0001 0.0001 0 0 0
Endoplasmic Reticulum 0.6015 0.442 0.4815 0.4407 0.4537 0.4381 0.6977 0.6889 0.723 0.7239 0.6552 0.7584 0.7055 0.6579 0.6577 0.7824 0.9822 0.7694
Endosome 0.0267 0.0238 0.0261 0.0231 0.0163 0.0167 0.0248 0.0114 0.0166 0.0126 0.0256 0.0172 0.0091 0.0134 0.0084 0.0058 0.0005 0.0085
Golgi 0.0033 0.0012 0.0042 0.0028 0.0049 0.0008 0.0048 0.0012 0.0047 0.0113 0.0021 0.002 0.0158 0.0021 0.0026 0.0043 0 0.0046
Lipid Particles 0.0086 0.0057 0.01 0.0134 0.0161 0.0037 0.0242 0.0119 0.0112 0.0047 0.0059 0.0077 0.0051 0.0151 0.0152 0.0212 0.0001 0.0027
Mitochondria 0.0025 0.0014 0.0038 0.0025 0.0083 0.001 0.0006 0.0004 0.0005 0.0008 0.0005 0.0005 0.0036 0.0005 0.0014 0.0143 0 0.0014
None 0.0471 0.0754 0.1084 0.1244 0.116 0.1274 0.0349 0.0167 0.0197 0.0097 0.0515 0.07 0.059 0.0246 0.0161 0.0023 0 0.016
Nuclear Periphery 0.0286 0.0269 0.0396 0.0372 0.0379 0.021 0.0104 0.0081 0.0183 0.0104 0.01 0.0085 0.0262 0.0128 0.0097 0.0125 0.0095 0.004
Nucleolus 0.0007 0.0014 0.0035 0.0035 0.0023 0.001 0.0001 0.0003 0.001 0 0 0 0.0003 0.0001 0.0005 0.0001 0 0
Nucleus 0.0105 0.0178 0.0318 0.023 0.0249 0.0154 0.0021 0.0055 0.0022 0.001 0.0014 0.0012 0.0148 0.0012 0.0023 0.0011 0.0007 0.0008
Peroxisomes 0.0038 0.0003 0.0056 0.003 0.0201 0.0003 0.0026 0.0001 0.0004 0.0234 0.0001 0.0001 0.0003 0.0001 0.0073 0.009 0 0.0001
Punctate Nuclear 0.0033 0.0096 0.0219 0.0293 0.0183 0.0192 0.0011 0.001 0.0006 0.0003 0.0005 0.0003 0.0049 0.0005 0.0183 0.0001 0 0.0001
Vacuole 0.008 0.0086 0.0085 0.0063 0.0058 0.0055 0.0062 0.0083 0.0068 0.0042 0.0048 0.0056 0.0054 0.005 0.0041 0.0045 0.001 0.0016
Vacuole Periphery 0.0018 0.001 0.0014 0.0016 0.0016 0.0008 0.0005 0.0004 0.0007 0.0005 0.0005 0.0005 0.0009 0.0005 0.0003 0.0009 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.3622 8.9671 21.7362 17.4831 25.1441 12.6988 15.7154 21.8256 23.9568 19.7319
Translational Efficiency 1.2444 1.2334 0.7262 1.1598 0.6644 0.9144 0.9267 0.7782 0.7049 0.8351

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Alpha-1,2-mannosidase; involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation
Localization
Cell Percentages ER (82%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Mns1

Mns1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mns1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available