ORF | |
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Human Ortholog | |
Description | Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 0 | 1 | 3 |
Bud | 0 | 0 | 7 | 1 | 1 | 0 | – | 5 | 6 | 10 | 11 | 10 | 0 | 1 | 0 | 0 | 1 | 0 | 4 | 3 | 2 | 1 | 5 | 11 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 3 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 1 | 1 | 2 | 8 | – | 4 | 1 | 1 | 3 | 5 | 0 | 0 | 1 | 5 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 310 | 376 | 271 | 227 | 253 | 335 | – | 377 | 313 | 345 | 209 | 298 | 289 | 372 | 322 | 166 | 175 | 146 | 286 | 361 | 257 | 137 | 197 | 240 |
Endoplasmic Reticulum | 2 | 0 | 1 | 0 | 0 | 1 | – | 1 | 1 | 2 | 2 | 6 | 1 | 1 | 1 | 4 | 5 | 5 | 0 | 0 | 1 | 0 | 2 | 2 |
Endosome | 0 | 2 | 2 | 1 | 1 | 1 | – | 11 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 2 | 2 | 4 | 3 | 3 | 3 | 1 | 2 |
Golgi | 0 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 1 | 3 | 0 | 1 | 5 | 2 |
Mitochondria | 4 | 3 | 24 | 47 | 19 | 112 | – | 236 | 238 | 309 | 225 | 319 | 0 | 0 | 0 | 3 | 2 | 0 | 3 | 4 | 8 | 2 | 2 | 2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 8 | 3 | 10 | 3 | 13 | 0 | 0 | 1 | 1 | 2 | 7 | 1 | 1 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 5 | 4 | 4 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 1 | 0 | 0 | – | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 5 | 2 | 3 | 1 | 1 | 7 |
Vac/Vac Membrane | 2 | 2 | 2 | 1 | 7 | 19 | – | 42 | 30 | 35 | 17 | 24 | 0 | 0 | 1 | 6 | 2 | 10 | 2 | 4 | 2 | 1 | 1 | 2 |
Unique Cell Count | 317 | 384 | 286 | 238 | 268 | 398 | 562 | 471 | 562 | 379 | 529 | 290 | 375 | 324 | 173 | 183 | 156 | 318 | 391 | 293 | 152 | 226 | 283 | |
Labelled Cell Count | 318 | 387 | 309 | 279 | 284 | 476 | 690 | 601 | 719 | 472 | 680 | 291 | 376 | 326 | 187 | 192 | 173 | 318 | 391 | 293 | 152 | 226 | 283 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 5.8 | 4.3 | 4.6 | 5.3 | 4.7 | – | 4.3 | 3.9 | 4.0 | 3.9 | 3.8 | 5.8 | 5.8 | 5.7 | 8.1 | 7.2 | 7.3 | 6.0 | 6.5 | 6.3 |
Std Deviation (1e-4) | 0.7 | 0.9 | 1.6 | 1.5 | 1.9 | 3.4 | – | 1.2 | 1.4 | 0.9 | 1.3 | 1.4 | 1.0 | 1.0 | 1.3 | 2.3 | 1.7 | 1.5 | 1.3 | 1.9 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.12 | 0.31 | 0.15 | – | 0.01 | -0.12 | -0.08 | -0.14 | -0.15 | 0.45 | 0.45 | 0.43 | 0.93 | 0.76 | 0.77 | 0.5 | 0.61 | 0.56 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.3 | -0.2 | -4.3 | – | -9.0 | -9.0 | -10.3 | -11.3 | -11.4 | 3.6 | 3.5 | 3.5 | 0.6 | 0.4 | -0.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | -0.6 | 0 | – | 0 | 0 | 0 | 0 | 0 | -5.0 | -5.7 | -5.3 | -2.9 | -3.4 | -3.7 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 3.0 | – | 4.2 | 3.8 | 3.7 | 2.9 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.6916 | 0.34 | 0.1861 | -0.2396 | 0.1546 | 0.1449 | 0.5764 | 0.4575 | 0.3362 | 0.1648 | 0.0868 | 0.3824 | 2.2278 | 1.9238 | 1.8573 | 2.1101 | 1.7597 | 1.5971 |
Actin | 0.0228 | 0.001 | 0.0072 | 0.0093 | 0.0072 | 0.0133 | 0.0056 | 0.0024 | 0.0101 | 0.0318 | 0.0022 | 0.0107 | 0.0002 | 0.0003 | 0.0005 | 0.0177 | 0.0004 | 0.0006 |
Bud | 0.0005 | 0.0001 | 0.0002 | 0.0003 | 0.001 | 0 | 0.0004 | 0.001 | 0.0012 | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0003 | 0.0008 | 0.0008 | 0.0001 |
Bud Neck | 0.0029 | 0.0001 | 0.0004 | 0.0002 | 0.0091 | 0.0003 | 0.0085 | 0.0002 | 0.0005 | 0.0012 | 0.0002 | 0.0006 | 0 | 0.0002 | 0.0006 | 0.0127 | 0.0002 | 0.0095 |
Bud Periphery | 0.0008 | 0.0001 | 0.0005 | 0.0006 | 0.0012 | 0 | 0.0006 | 0.0009 | 0.0014 | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0003 | 0.0012 | 0.0006 | 0.0001 |
Bud Site | 0.0045 | 0.001 | 0.0022 | 0.0007 | 0.025 | 0 | 0.0122 | 0.0138 | 0.0045 | 0.001 | 0.0002 | 0.0002 | 0.0001 | 0.0041 | 0.0033 | 0.0182 | 0.0001 | 0.0001 |
Cell Periphery | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0008 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0 | 0 |
Cytoplasm | 0.5157 | 0.4859 | 0.5685 | 0.4506 | 0.3332 | 0.6477 | 0.5352 | 0.53 | 0.538 | 0.4513 | 0.5475 | 0.5878 | 0.5805 | 0.4748 | 0.5661 | 0.4911 | 0.4451 | 0.6939 |
Cytoplasmic Foci | 0.0407 | 0.0099 | 0.0165 | 0.0102 | 0.0602 | 0.0071 | 0.0211 | 0.0075 | 0.0386 | 0.0223 | 0.018 | 0.0196 | 0.0061 | 0.004 | 0.0062 | 0.0082 | 0.0058 | 0.0062 |
Eisosomes | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0005 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0032 | 0.0032 | 0.0056 | 0.0039 | 0.0032 | 0.0023 | 0.0042 | 0.0015 | 0.0035 | 0.0017 | 0.0019 | 0.0012 | 0.0061 | 0.001 | 0.002 | 0.002 | 0.0018 | 0.0022 |
Endosome | 0.0101 | 0.0036 | 0.0067 | 0.0129 | 0.0346 | 0.0021 | 0.023 | 0.0042 | 0.007 | 0.0026 | 0.011 | 0.0032 | 0.0013 | 0.0007 | 0.0012 | 0.0033 | 0.0011 | 0.0015 |
Golgi | 0.004 | 0.0003 | 0.0008 | 0.0066 | 0.0044 | 0.001 | 0.0077 | 0.0003 | 0.003 | 0.0027 | 0.0082 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0001 |
Lipid Particles | 0.0106 | 0.0004 | 0.0009 | 0.0287 | 0.0192 | 0.0022 | 0.0111 | 0.0005 | 0.0032 | 0.0028 | 0.0046 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0001 |
Mitochondria | 0.0025 | 0.0001 | 0.0015 | 0.0355 | 0.0118 | 0.0002 | 0.0034 | 0.0058 | 0.0048 | 0.0013 | 0.0019 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0.0007 | 0.0002 | 0.0002 |
None | 0.3349 | 0.4894 | 0.3753 | 0.4213 | 0.4182 | 0.3182 | 0.3336 | 0.4229 | 0.3635 | 0.4591 | 0.3896 | 0.3685 | 0.4017 | 0.5108 | 0.4094 | 0.418 | 0.5377 | 0.2799 |
Nuclear Periphery | 0.0076 | 0.0008 | 0.005 | 0.0022 | 0.0071 | 0.0005 | 0.0032 | 0.0007 | 0.0021 | 0.0012 | 0.0007 | 0.0006 | 0.0009 | 0.0004 | 0.0007 | 0.0021 | 0.0009 | 0.0007 |
Nucleolus | 0.0007 | 0.0001 | 0.0002 | 0.0004 | 0.0013 | 0 | 0.0016 | 0.0004 | 0.0004 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0017 | 0.0006 | 0.0001 | 0 |
Nucleus | 0.0049 | 0.0014 | 0.0033 | 0.0017 | 0.004 | 0.0013 | 0.0048 | 0.0028 | 0.0034 | 0.0024 | 0.0016 | 0.0025 | 0.0013 | 0.0014 | 0.004 | 0.0177 | 0.0022 | 0.0032 |
Peroxisomes | 0.0227 | 0.0001 | 0.0002 | 0.01 | 0.0317 | 0.0026 | 0.0137 | 0.0006 | 0.0101 | 0.0136 | 0.0102 | 0.0009 | 0.0001 | 0.0001 | 0.0005 | 0.0006 | 0.0001 | 0.0002 |
Punctate Nuclear | 0.0078 | 0.0013 | 0.003 | 0.0031 | 0.0223 | 0.0006 | 0.0029 | 0.0012 | 0.0028 | 0.0024 | 0.0006 | 0.0023 | 0.0006 | 0.0007 | 0.0012 | 0.0018 | 0.0022 | 0.001 |
Vacuole | 0.0015 | 0.0009 | 0.0012 | 0.0011 | 0.0035 | 0.0004 | 0.0051 | 0.0029 | 0.0012 | 0.0005 | 0.0008 | 0.0005 | 0.0005 | 0.0004 | 0.0005 | 0.0011 | 0.0005 | 0.0005 |
Vacuole Periphery | 0.001 | 0.0001 | 0.0005 | 0.0006 | 0.0016 | 0.0001 | 0.0008 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.9094 | 5.5727 | 6.2028 | 6.7208 | 18.1857 | 12.4609 | 12.6778 | 7.6059 | 3.9702 | 10.4399 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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