Standard name
Human Ortholog
Description Vacuolar transporter; exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0.06 0.09 0.05 0.05 0
Bud 0 0 0 0 0 0 0 0 – 0 0.05 0.05 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0.11 0.11 0.09 0.07 0 0 0 – 0 0 0 0.07 0.11 0.12 – – – 0 0.05 0.08 0.11 0.12 0.12
Cytoplasm 0.69 0.52 0.68 0.62 0.62 0.57 0.59 0.61 – 0.57 0.62 0.6 0.81 0.9 0.96 – – – 0.17 0.18 0.1 0.1 0.14 0.18
Endoplasmic Reticulum 0.17 0.33 0.16 0.11 0.12 0 0 0 – 0 0 0 0.15 0.06 0 – – – 0.54 0.51 0.55 0.46 0.41 0.38
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.08 0.12 0.11 0.32 0.26 0.5 0.5 0.52 – 0.6 0.57 0.57 0 0 0 – – – 0.1 0.06 0.11 0.19 0.15 0.16
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 9 13 6 0 0 0 1 – 0 0 0 3 4 2 – – – 7 12 35 12 19 18
Bud 1 5 6 9 9 7 12 14 – 19 29 44 0 1 0 – – – 1 3 1 1 3 4
Bud Neck 1 0 3 0 0 0 1 0 – 0 0 1 0 1 2 – – – 4 8 6 7 8 10
Bud Site 0 0 0 0 0 0 0 3 – 0 2 1 0 0 0 – – – – – – – – –
Cell Periphery 4 16 35 23 23 12 14 11 – 16 14 23 9 17 27 – – – 5 9 29 24 42 52
Cytoplasm 92 75 213 153 198 218 343 401 – 340 371 531 100 137 220 – – – 31 34 38 22 51 74
Endoplasmic Reticulum 23 48 49 27 38 13 11 7 – 9 1 8 19 9 7 – – – 99 96 214 105 146 159
Endosome 1 0 2 1 8 2 4 1 – 0 1 2 0 0 0 – – – 1 2 2 0 1 3
Golgi 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 3 0 0 0 4 1
Mitochondria 11 17 35 79 82 191 286 338 – 358 342 509 0 0 0 – – – 19 11 43 43 55 65
Nucleus 0 0 0 2 0 0 0 2 – 3 1 2 0 0 1 – – – 0 0 0 0 0 0
Nuclear Periphery 1 0 1 0 1 0 1 2 – 2 1 2 0 0 0 – – – 0 1 0 0 1 1
Nucleolus 0 0 0 0 0 0 0 2 – 2 1 0 0 0 0 – – – 0 0 0 0 0 1
Peroxisomes 0 0 0 1 0 0 0 0 – 0 0 1 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 1 0 0 0 1 1 2 4 – 3 9 9 0 0 0 – – – 0 0 0 0 0 1
Vac/Vac Membrane 2 1 10 3 19 15 24 14 – 11 6 6 0 2 0 – – – 5 7 8 7 18 16
Unique Cell Count 134 144 313 248 321 385 577 653 593 600 890 123 152 229 184 191 390 228 360 420
Labelled Cell Count 143 171 367 304 379 459 698 800 763 778 1139 131 171 259 184 191 390 228 360 420


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.2 4.5 4.7 4.7 3.8 3.6 3.7 – 3.2 3.2 3.2 6.2 6.3 6.0 – 4.3 – 6.4 6.9 6.8
Std Deviation (1e-4) 0.8 0.7 0.9 1.3 1.0 1.4 0.9 0.9 – 0.9 0.9 0.7 0.7 0.9 0.7 – 0.9 – 1.0 1.6 1.5
Intensity Change (Log2) – – – 0.07 0.05 -0.23 -0.3 -0.28 – -0.51 -0.48 -0.49 0.46 0.48 0.42 – -0.07 – 0.51 0.62 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 -0.8 -2.3 – – –
Bud 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 – 0 0 0 -1.2 0 0.2 – – –
Cytoplasm -1.6 -1.7 -3.1 -2.5 -2.0 – -3.1 -1.9 -2.6 2.8 5.2 8.0 – – –
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 -0.1 -3.0 -4.8 – – –
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Mitochondria 0 0 0 0 0 – 0 0 0 -3.9 -4.3 -5.2 – – –
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Vacuole -1.6 1.6 0.5 0.7 -1.0 – -1.3 -2.4 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2857 1.6434 1.5218 1.4077 1.0415 1.489 1.6298 2.1727 1.5695 1.599 0.8461 1.9874 1.4223 2.0324 1.4266 1.7376 1.5327 1.5311
Actin 0.0132 0.0101 0.0067 0.0027 0.013 0.0125 0.0356 0.0089 0.0122 0.007 0.0216 0.0107 0.0555 0.0123 0.0169 0.0162 0.0068 0.0079
Bud 0.0001 0.0012 0.0041 0.0007 0.0003 0.0002 0.0004 0.0008 0.0007 0.0005 0.0004 0.0002 0.0005 0.0004 0.0018 0.0393 0.0003 0.0003
Bud Neck 0.0008 0.0012 0.0021 0.0058 0.0032 0.0088 0.001 0.003 0.0083 0.0141 0.0054 0.0141 0.0041 0.0032 0.0087 0.0021 0.0055 0.017
Bud Periphery 0.0004 0.002 0.0019 0.0015 0.001 0.0006 0.0007 0.0028 0.0026 0.0025 0.0013 0.0015 0.0019 0.0009 0.0031 0.016 0.0007 0.0016
Bud Site 0.0011 0.0402 0.0114 0.0023 0.0005 0.0005 0.0022 0.0522 0.0171 0.0054 0.0009 0.0005 0.0055 0.0443 0.0172 0.0026 0.0032 0.0006
Cell Periphery 0.0101 0.0062 0.0097 0.007 0.0041 0.0051 0.0136 0.0245 0.0201 0.0103 0.0048 0.0053 0.0133 0.0249 0.0293 0.0201 0.0026 0.0091
Cytoplasm 0.4997 0.558 0.5463 0.5512 0.5147 0.5396 0.4233 0.4843 0.4758 0.4633 0.5295 0.5185 0.3645 0.5366 0.54 0.506 0.5607 0.5898
Cytoplasmic Foci 0.0143 0.0174 0.0167 0.0285 0.0486 0.0315 0.0214 0.0161 0.0296 0.0112 0.0124 0.0151 0.025 0.0081 0.0211 0.0102 0.0219 0.0181
Eisosomes 0.001 0.0005 0.0008 0.0003 0.0005 0.0004 0.0011 0.0006 0.0016 0.0012 0.0011 0.0004 0.0014 0.0005 0.0012 0.0052 0.0005 0.0003
Endoplasmic Reticulum 0.2029 0.1302 0.1438 0.1593 0.1941 0.1545 0.1462 0.1572 0.1566 0.1475 0.1716 0.1709 0.2112 0.1514 0.203 0.166 0.0976 0.1954
Endosome 0.0101 0.0154 0.0166 0.0139 0.0128 0.01 0.0275 0.0252 0.0066 0.0093 0.0076 0.0124 0.0185 0.0103 0.0137 0.007 0.0213 0.0073
Golgi 0.0036 0.0043 0.003 0.002 0.0052 0.0024 0.0097 0.0064 0.0041 0.0024 0.0032 0.0018 0.0104 0.0025 0.0076 0.0087 0.0083 0.0032
Lipid Particles 0.0119 0.0106 0.0119 0.0097 0.0068 0.005 0.0205 0.0075 0.0092 0.0117 0.0064 0.0095 0.0371 0.0123 0.0181 0.0033 0.0294 0.0099
Mitochondria 0.0008 0.0012 0.0044 0.0023 0.0025 0.0015 0.0047 0.0015 0.0112 0.0007 0.0008 0.0008 0.003 0.0006 0.0023 0.0087 0.0345 0.0016
None 0.2181 0.1859 0.202 0.191 0.1688 0.2151 0.2146 0.1873 0.2165 0.3026 0.2205 0.2302 0.2064 0.1817 0.0966 0.1722 0.1812 0.1264
Nuclear Periphery 0.0038 0.0039 0.0056 0.0032 0.0038 0.0023 0.0152 0.0092 0.0025 0.0021 0.0028 0.0019 0.0081 0.0028 0.0043 0.0038 0.0017 0.0018
Nucleolus 0.0003 0.0004 0.0011 0.0006 0.0002 0.0002 0.0032 0.0003 0.0003 0.0003 0.0002 0.0001 0.0009 0.0002 0.0006 0.0008 0.0048 0.0001
Nucleus 0.0014 0.003 0.0033 0.0043 0.0019 0.0016 0.0112 0.0027 0.0012 0.0016 0.0013 0.001 0.0024 0.0012 0.0017 0.0034 0.0013 0.0011
Peroxisomes 0.0008 0.0005 0.001 0.0005 0.013 0.0019 0.0271 0.0005 0.0179 0.0002 0.0004 0.0003 0.0152 0.0002 0.0049 0.0004 0.0131 0.003
Punctate Nuclear 0.0013 0.0016 0.0017 0.0083 0.0021 0.0031 0.0039 0.0013 0.0018 0.0013 0.0006 0.0005 0.0109 0.0006 0.0028 0.0025 0.0015 0.0025
Vacuole 0.0042 0.0056 0.0053 0.0047 0.0026 0.0028 0.0156 0.0072 0.0039 0.0044 0.0068 0.004 0.0038 0.0048 0.0047 0.0049 0.0026 0.0029
Vacuole Periphery 0.0003 0.0004 0.0006 0.0004 0.0004 0.0003 0.0012 0.0007 0.0002 0.0003 0.0005 0.0002 0.0006 0.0003 0.0005 0.0005 0.0007 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.4754 16.1312 22.1798 24.747 20.9878 22.8091 25.6647 24.7687 23.8301 28.0192
Translational Efficiency 0.9448 0.959 0.8074 0.7275 0.9654 1.2175 0.8002 0.8957 0.8524 0.7555

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Vacuolar transporter; exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
Localization
Cell Percentages ER (45%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Avt3

Avt3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Avt3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available