ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | – | 1 | 3 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 6 | 1 | 0 | 4 | 10 | 11 |
Bud | 3 | 3 | – | 1 | 2 | 5 | 0 | – | 1 | 4 | 6 | 9 | 1 | 3 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 2 |
Bud Neck | 3 | 0 | – | 3 | 4 | 0 | 7 | – | 2 | 2 | 0 | 0 | 4 | 1 | 1 | 2 | 0 | 1 | 13 | 7 | 0 | 11 | 22 | 22 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 30 | 6 | – | 40 | 11 | 5 | 11 | – | 17 | 21 | 22 | 15 | 101 | 166 | 170 | 44 | 41 | 26 | 32 | 8 | 6 | 60 | 155 | 129 |
Cytoplasm | 247 | 66 | – | 198 | 285 | 182 | 385 | – | 256 | 294 | 228 | 249 | 301 | 562 | 592 | 204 | 168 | 148 | 177 | 72 | 10 | 156 | 292 | 337 |
Endoplasmic Reticulum | 1 | 0 | – | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 13 | 10 | 13 | 19 | 1 | 0 | 6 | 14 | 21 |
Endosome | 0 | 0 | – | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 3 |
Golgi | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 4 | 0 |
Mitochondria | 3 | 1 | – | 1 | 0 | 19 | 16 | – | 67 | 61 | 80 | 99 | 0 | 0 | 2 | 2 | 0 | 2 | 2 | 1 | 0 | 2 | 5 | 4 |
Nucleus | 1 | 0 | – | 0 | 1 | 4 | 6 | – | 5 | 1 | 5 | 5 | 0 | 0 | 0 | 4 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | – | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | – | 0 | 0 | 0 | 2 | – | 2 | 2 | 1 | 6 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 0 | 0 | – | 0 | 0 | 0 | 3 | – | 0 | 0 | 0 | 2 | 0 | 3 | 1 | 1 | 2 | 1 | 1 | 0 | 0 | 1 | 2 | 1 |
Unique Cell Count | 254 | 67 | 202 | 292 | 188 | 401 | 294 | 331 | 273 | 312 | 306 | 568 | 601 | 216 | 173 | 157 | 260 | 94 | 19 | 253 | 525 | 542 | ||
Labelled Cell Count | 291 | 76 | 245 | 307 | 216 | 432 | 351 | 387 | 343 | 386 | 408 | 737 | 773 | 274 | 222 | 198 | 260 | 94 | 19 | 253 | 525 | 542 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 5.7 | – | 5.5 | 5.6 | 3.8 | 5.0 | – | 4.1 | 4.3 | 4.3 | 4.4 | 6.1 | 6.2 | 6.4 | 6.6 | 7.3 | 6.5 | 5.6 | 5.9 | 5.9 |
Std Deviation (1e-4) | 0.6 | 0.9 | – | 1.0 | 1.3 | 1.0 | 1.8 | – | 1.1 | 1.2 | 1.4 | 2.2 | 0.9 | 1.1 | 1.4 | 1.2 | 1.2 | 1.0 | 1.3 | 1.5 | 1.0 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
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G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.5974 | 0.5487 | 0.2051 | -0.2269 | -0.4608 | 0.1539 | 0.6729 | 1.1092 | 0.7885 | 1.0944 | 1.1514 | 1.1329 | 2.7862 | 2.6431 | 2.8602 | 2.833 | 3.1195 | 2.7933 |
Actin | 0.062 | 0.0006 | 0.0072 | 0.0012 | 0.0197 | 0.0245 | 0.0419 | 0.0026 | 0.0041 | 0.004 | 0.0367 | 0.0073 | 0.0307 | 0.0037 | 0.0006 | 0.0029 | 0.0082 | 0.0062 |
Bud | 0.0008 | 0.0002 | 0.0018 | 0.0004 | 0.0002 | 0.0009 | 0.0007 | 0.001 | 0.0011 | 0.0003 | 0.0005 | 0.0003 | 0.0009 | 0.0002 | 0.0004 | 0.0002 | 0.0026 | 0.0002 |
Bud Neck | 0.0041 | 0.0013 | 0.0019 | 0.0015 | 0.001 | 0.0153 | 0.0035 | 0.0012 | 0.0104 | 0.0054 | 0.001 | 0.0171 | 0.001 | 0.0006 | 0.0014 | 0.0007 | 0.0011 | 0.0207 |
Bud Periphery | 0.0011 | 0.0005 | 0.0093 | 0.0009 | 0.0004 | 0.0029 | 0.0013 | 0.0012 | 0.0017 | 0.0007 | 0.0012 | 0.0007 | 0.0052 | 0.0005 | 0.0016 | 0.0005 | 0.0031 | 0.0004 |
Bud Site | 0.01 | 0.0258 | 0.0099 | 0.0018 | 0.0008 | 0.0008 | 0.0056 | 0.0297 | 0.0269 | 0.0048 | 0.0003 | 0.0003 | 0.0046 | 0.0097 | 0.0047 | 0.0006 | 0.0073 | 0.0014 |
Cell Periphery | 0.0142 | 0.006 | 0.0083 | 0.0037 | 0.0014 | 0.0046 | 0.0105 | 0.0105 | 0.0093 | 0.0072 | 0.0017 | 0.0039 | 0.0126 | 0.0149 | 0.0121 | 0.0127 | 0.0054 | 0.0031 |
Cytoplasm | 0.365 | 0.3203 | 0.453 | 0.42 | 0.299 | 0.5221 | 0.541 | 0.6209 | 0.7251 | 0.5744 | 0.6169 | 0.7498 | 0.5578 | 0.6055 | 0.694 | 0.6109 | 0.6144 | 0.7207 |
Cytoplasmic Foci | 0.0387 | 0.0023 | 0.0079 | 0.0279 | 0.0207 | 0.0165 | 0.0202 | 0.0083 | 0.0035 | 0.0165 | 0.0091 | 0.0045 | 0.0352 | 0.0047 | 0.0015 | 0.003 | 0.0098 | 0.002 |
Eisosomes | 0.0009 | 0.0005 | 0.0009 | 0.0008 | 0.0002 | 0.0004 | 0.0005 | 0.0006 | 0.0006 | 0.0006 | 0.0012 | 0.0003 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.009 | 0.0032 | 0.0078 | 0.0096 | 0.0039 | 0.0028 | 0.0054 | 0.0026 | 0.006 | 0.0038 | 0.0099 | 0.0025 | 0.0053 | 0.0026 | 0.0044 | 0.0033 | 0.0039 | 0.0022 |
Endosome | 0.0422 | 0.0002 | 0.008 | 0.0041 | 0.0259 | 0.0015 | 0.0102 | 0.0021 | 0.0007 | 0.0097 | 0.0277 | 0.0026 | 0.0218 | 0.0025 | 0.0006 | 0.0046 | 0.007 | 0.0007 |
Golgi | 0.0172 | 0.0001 | 0.0034 | 0.0006 | 0.0088 | 0.0013 | 0.003 | 0.0003 | 0.0005 | 0.0069 | 0.0111 | 0.0008 | 0.0048 | 0.0108 | 0.0001 | 0.0065 | 0.0049 | 0.0003 |
Lipid Particles | 0.0254 | 0.0003 | 0.0103 | 0.0035 | 0.0208 | 0.0039 | 0.0102 | 0.001 | 0.0008 | 0.0194 | 0.0023 | 0.0007 | 0.0117 | 0.0008 | 0.0005 | 0.0092 | 0.0058 | 0.0005 |
Mitochondria | 0.0094 | 0.0001 | 0.0036 | 0.0004 | 0.0019 | 0.0008 | 0.0007 | 0.0002 | 0.0002 | 0.0073 | 0.0068 | 0.0002 | 0.001 | 0.0019 | 0.0001 | 0.0269 | 0.0028 | 0.0001 |
None | 0.326 | 0.636 | 0.4535 | 0.5089 | 0.5489 | 0.3929 | 0.3236 | 0.3134 | 0.2033 | 0.3043 | 0.2625 | 0.2047 | 0.2674 | 0.3368 | 0.2645 | 0.3133 | 0.2785 | 0.2394 |
Nuclear Periphery | 0.0122 | 0.0002 | 0.0031 | 0.0008 | 0.008 | 0.0007 | 0.0013 | 0.0004 | 0.0005 | 0.0005 | 0.0061 | 0.0006 | 0.0074 | 0.0002 | 0.0051 | 0.0002 | 0.0009 | 0.0005 |
Nucleolus | 0.0023 | 0.0003 | 0.0005 | 0.001 | 0.0005 | 0.0001 | 0.0006 | 0.0003 | 0.001 | 0.001 | 0.0001 | 0.0002 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 |
Nucleus | 0.0144 | 0.0008 | 0.0026 | 0.0015 | 0.0013 | 0.0005 | 0.0013 | 0.0007 | 0.0014 | 0.0008 | 0.0008 | 0.0006 | 0.0037 | 0.0007 | 0.0063 | 0.0004 | 0.0008 | 0.0003 |
Peroxisomes | 0.0125 | 0.0001 | 0.0032 | 0.0006 | 0.0097 | 0.0057 | 0.0068 | 0.0002 | 0.0002 | 0.0271 | 0.0007 | 0.0006 | 0.0083 | 0.0014 | 0 | 0.0024 | 0.0406 | 0.0002 |
Punctate Nuclear | 0.0266 | 0.0004 | 0.0015 | 0.0085 | 0.0255 | 0.0009 | 0.0088 | 0.0011 | 0.0011 | 0.0034 | 0.0009 | 0.002 | 0.0163 | 0.0002 | 0.0003 | 0.0001 | 0.001 | 0.0004 |
Vacuole | 0.0043 | 0.0008 | 0.002 | 0.0022 | 0.001 | 0.001 | 0.0027 | 0.0016 | 0.0016 | 0.0016 | 0.0014 | 0.0006 | 0.0027 | 0.0017 | 0.0013 | 0.0012 | 0.0014 | 0.0004 |
Vacuole Periphery | 0.0016 | 0 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.001 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0004 | 0.0002 | 0.0001 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.9924 | 14.2953 | 15.9116 | 12.571 | 15.5501 | 9.9891 | 13.0989 | 15.2429 | 14.322 | 14.5593 |
Translational Efficiency | 0.9382 | 1.0389 | 0.9045 | 0.8937 | 1.0486 | 1.4069 | 1.1868 | 0.8519 | 0.9988 | 1.0597 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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