Standard name
Human Ortholog
Description S-glutathionyl-(chloro)hydroquinone reductase (GS-HQR); glutathione transferase involved in cell-surface biosynthesis and architecture; catalyzes glutathione (GSH)-dependent reduction of GS-trichloro-p-hydroquinone to trichloro-p-hydroquinone; expression upregulated upon exposure to genotoxic agents, such as methyl methanesulfonate, cisplatin and bleomycin; not an essential gene; similar to YGR154C; green fluorescent protein (GFP)-fusion protein localizes to cytoplasm

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.07 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.05 0 0 0 0 0 0 0
Cytoplasm 0.92 0.97 0.95 0.98 0.97 0.88 0.74 0.77 0.59 0.62 0.56 0.54 0.96 0.98 0.99 0.98 0.92 0.96 0.78 0.85 0.7 0.9 0.85 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0 0.05 0.05 0.05 0.2 0.39 0.27 0.55 0.47 0.55 0.53 0 0 0 0 0 0 0.09 0.06 0.14 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.08 0.06 0.09 0 0.05 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 2 1
Bud 1 0 4 0 1 0 6 4 6 6 9 6 0 0 0 0 0 0 2 0 0 0 1 3
Bud Neck 0 0 1 5 3 0 1 1 0 0 0 2 1 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0
Cell Periphery 0 1 0 4 1 2 0 0 0 1 2 1 0 0 1 15 7 4 1 0 1 0 0 0
Cytoplasm 220 142 56 165 226 185 197 165 110 149 92 116 92 147 161 133 135 141 196 125 66 101 150 139
Endoplasmic Reticulum 2 2 0 0 0 0 0 0 4 0 0 0 1 0 0 4 10 13 1 0 1 1 2 0
Endosome 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 2 0 7 2 0 0 3 2
Golgi 1 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0
Mitochondria 39 3 3 8 11 43 103 58 101 112 89 114 1 1 0 1 4 6 22 8 13 3 6 6
Nucleus 0 0 2 1 1 0 3 4 2 2 4 7 1 0 0 0 1 1 0 0 0 0 0 0
Nuclear Periphery 2 1 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 1 2 2 0 2 0 2 0 0 0 0 0 0 3 1 1 2 1 1
Vac/Vac Membrane 1 1 0 0 0 7 12 5 7 18 9 20 0 7 5 3 4 3 4 2 1 0 1 1
Unique Cell Count 238 147 59 168 232 211 267 215 185 239 163 214 96 150 163 136 146 147 251 147 94 112 176 163
Labelled Cell Count 266 150 67 184 245 241 325 241 231 292 205 268 96 155 167 156 164 170 251 147 94 112 176 163


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 5.0 4.3 4.7 5.2 4.3 4.1 4.6 4.0 4.5 4.2 4.6 5.2 6.6 6.9 6.7 6.4 6.4 4.5 5.7 5.9
Std Deviation (1e-4) 0.7 1.4 1.2 2.1 1.9 1.6 1.6 1.8 1.4 1.5 1.5 1.5 1.5 2.6 2.4 1.4 1.7 1.9 1.7 2.9 2.4
Intensity Change (Log2) 0.11 0.28 0.01 -0.06 0.11 -0.1 0.05 -0.04 0.08 0.28 0.61 0.69 0.63 0.56 0.56 0.06 0.4 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 1.0 -1.6 -3.5 -3.1 -5.1 -4.8 -5.4 -5.7 0.3 1.2 1.7 1.1 -0.6 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.621 1.3513 0.9728 0.982 0.5652 0.9184 0.2285 0.4991 0.4056 0.0035 0.9661 0.4847 0.5492 -0.2208 -0.4407 -0.5022 -0.3178 -0.6832
Actin 0.0292 0.0004 0.0033 0.0026 0.0074 0.0088 0.0252 0.0054 0.0112 0.0025 0.0493 0.012 0.0698 0.0021 0.0215 0.0229 0.0059 0.0184
Bud 0.0042 0.0015 0.0064 0.0147 0.0011 0.001 0.0008 0.0088 0.0006 0.0135 0.0004 0.0003 0.0045 0.006 0.0045 0.0005 0.0004 0.0023
Bud Neck 0.0013 0.0003 0.0009 0.0006 0.0003 0.0035 0.0044 0.0011 0.0005 0.0005 0.0004 0.0021 0.011 0.0005 0.003 0.0011 0.0009 0.003
Bud Periphery 0.003 0.0004 0.0043 0.0037 0.0017 0.0021 0.0012 0.0046 0.0011 0.0119 0.0006 0.0006 0.006 0.0037 0.0068 0.0012 0.0009 0.0052
Bud Site 0.0045 0.0057 0.0046 0.0022 0.0014 0.001 0.0033 0.0103 0.0017 0.0032 0.0008 0.0004 0.021 0.0291 0.0099 0.0035 0.0019 0.003
Cell Periphery 0.0004 0.0002 0.0002 0.0004 0 0.0001 0.0005 0.0004 0.0002 0.0003 0.0001 0.0001 0.0008 0.0007 0.0005 0.0008 0.0002 0.0003
Cytoplasm 0.2741 0.4117 0.3266 0.3982 0.3547 0.395 0.2092 0.2996 0.2261 0.2421 0.1803 0.2766 0.1632 0.2696 0.2334 0.308 0.3506 0.3467
Cytoplasmic Foci 0.0295 0.0354 0.022 0.0365 0.0381 0.0345 0.0296 0.0126 0.0213 0.0353 0.0389 0.0259 0.0589 0.0125 0.0244 0.0215 0.0258 0.0192
Eisosomes 0.0004 0.0001 0.0001 0.0003 0.0001 0.0001 0.0006 0.0001 0.0002 0.0001 0.0002 0.0003 0.0014 0.0002 0.0004 0.0018 0.0002 0.0002
Endoplasmic Reticulum 0.0037 0.0044 0.002 0.0017 0.0008 0.0017 0.0016 0.0012 0.0015 0.0022 0.0012 0.0011 0.0092 0.0013 0.0034 0.0024 0.0022 0.0023
Endosome 0.0184 0.0062 0.0178 0.0104 0.0056 0.0087 0.0094 0.0036 0.0054 0.0088 0.0122 0.0057 0.04 0.002 0.018 0.0076 0.017 0.0077
Golgi 0.0056 0.0005 0.0016 0.0026 0.0041 0.0016 0.0046 0.0006 0.0019 0.006 0.0089 0.0024 0.0117 0.0007 0.0098 0.0064 0.0019 0.0037
Lipid Particles 0.0097 0.0008 0.0014 0.0167 0.0015 0.003 0.0166 0.0011 0.0031 0.0181 0.0297 0.0039 0.0306 0.0003 0.0088 0.013 0.0077 0.0028
Mitochondria 0.0104 0.001 0.003 0.0051 0.008 0.0062 0.0184 0.0015 0.0195 0.0034 0.0031 0.0052 0.0076 0.001 0.013 0.02 0.0077 0.0026
None 0.5746 0.5184 0.5923 0.4867 0.5617 0.516 0.6272 0.6391 0.6772 0.6182 0.539 0.6392 0.4626 0.664 0.6168 0.5685 0.5028 0.5711
Nuclear Periphery 0.0035 0.002 0.0017 0.0011 0.0005 0.0012 0.0013 0.0005 0.0017 0.0022 0.0046 0.0019 0.0273 0.0004 0.0044 0.0009 0.0096 0.0015
Nucleolus 0.0018 0.0003 0.0005 0.0005 0.0002 0.0003 0.0019 0.0005 0.0006 0.001 0.0007 0.0011 0.0022 0.0004 0.0007 0.0002 0.0073 0.0003
Nucleus 0.0084 0.0042 0.0031 0.0039 0.0017 0.0016 0.0057 0.0023 0.004 0.0074 0.0013 0.0022 0.0093 0.0021 0.0033 0.0015 0.0088 0.0023
Peroxisomes 0.008 0.0018 0.0017 0.0072 0.0078 0.0077 0.0267 0.0012 0.0125 0.0056 0.0866 0.0112 0.0388 0.0006 0.0059 0.0145 0.0047 0.0025
Punctate Nuclear 0.0065 0.0026 0.0042 0.0026 0.0026 0.0043 0.0087 0.0042 0.006 0.0141 0.0409 0.0066 0.0111 0.0014 0.0074 0.0015 0.0382 0.0031
Vacuole 0.0023 0.0018 0.0021 0.0019 0.0006 0.0012 0.0024 0.0011 0.0028 0.0029 0.0006 0.0008 0.0089 0.0016 0.0029 0.0015 0.0026 0.0014
Vacuole Periphery 0.0006 0.0003 0.0005 0.0005 0.0002 0.0003 0.0008 0.0002 0.0011 0.0007 0.0004 0.0003 0.0041 0.0001 0.0013 0.0005 0.0026 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.3922 5.3174 4.6034 10.1091 8.9976 27.7432 14.1131 16.0268 18.1524 14.7052
Translational Efficiency 1.4314 1.3761 1.1446 0.7026 0.7226 0.9454 1.0324 0.85 0.8873 0.7947

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description S-glutathionyl-(chloro)hydroquinone reductase (GS-HQR); glutathione transferase involved in cell-surface biosynthesis and architecture; catalyzes glutathione (GSH)-dependent reduction of GS-trichloro-p-hydroquinone to trichloro-p-hydroquinone; expression upregulated upon exposure to genotoxic agents, such as methyl methanesulfonate, cisplatin and bleomycin; not an essential gene; similar to YGR154C; green fluorescent protein (GFP)-fusion protein localizes to cytoplasm
Localization
Cell Percentages cytoplasm (31%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ecm4

Ecm4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ecm4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available