Standard name
Human Ortholog
Description Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.82 0.93 0.9 0.86 0.66 0.47 0.45 0.42 0.42 0.36 0.33 0.43 0.96 0.97 0.98 0.91 0.96 0.91 0.63 0.73 0.68 0.84 0.73 0.61
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0.18 0.21 0.25 0.65 0.64 0.66 0.75 0.76 0.8 0.73 0 0 0 0.09 0 0.07 0.09 0 0.15 0.05 0.11 0.12
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.07 0 0.06 0.1 0.09 0.1 0.07 0.05 0.06 0.08 0 0 0 0 0.06 0 0 0.08 0.08 0.06 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 1 0 3 5 10
Bud 6 0 1 3 2 6 6 11 4 9 12 13 5 1 3 0 0 1 2 1 0 2 3 19
Bud Neck 0 0 0 0 0 0 0 0 2 1 0 1 0 0 0 1 1 0 1 1 0 1 3 10
Bud Site 0 0 0 0 0 1 1 1 2 1 2 6 0 0 0 0 0 0
Cell Periphery 2 0 1 1 1 0 3 1 2 2 0 3 0 2 4 4 0 1 1 0 0 0 1 3
Cytoplasm 239 130 95 134 151 132 164 175 123 142 104 183 464 732 814 258 115 115 217 115 83 289 391 382
Endoplasmic Reticulum 4 1 0 0 5 3 1 6 3 0 2 2 7 5 6 16 2 5 5 1 5 5 8 21
Endosome 5 0 0 1 13 3 5 3 0 0 0 0 2 2 1 4 2 4 31 7 1 4 20 23
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 3 1 0 3 2 5
Mitochondria 35 6 19 32 58 184 232 274 221 301 251 307 3 4 0 25 4 9 29 4 18 16 59 72
Nucleus 0 0 0 0 2 2 4 5 7 8 8 11 3 0 2 0 1 1 2 8 0 1 1 1
Nuclear Periphery 2 1 1 0 0 0 2 3 0 5 4 3 0 0 0 0 0 1 2 0 1 0 2 2
Nucleolus 1 0 0 0 0 0 2 0 0 2 0 2 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 4 4 2 3 6 8 0 0 0 1 0 0 8 0 0 4 9 15
Vac/Vac Membrane 19 10 4 9 23 26 35 31 15 25 24 17 13 23 19 16 3 4 28 11 7 6 16 46
Unique Cell Count 290 140 106 156 228 281 361 415 295 397 315 422 485 752 833 282 120 127 347 157 123 346 534 628
Labelled Cell Count 313 148 121 180 255 358 459 514 381 499 413 556 497 770 851 326 129 141 347 157 123 346 534 628


Mitochondria, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.8 4.4 4.5 4.4 3.7 3.3 3.5 3.3 3.1 3.1 3.0 5.9 5.8 5.8 5.0 5.9 6.3 5.7 5.6 6.2
Std Deviation (1e-4) 0.7 1.0 0.7 1.1 1.1 1.1 1.1 0.7 0.9 1.0 1.0 1.2 1.0 1.2 1.3 1.2 1.5 1.2 1.8 1.3 1.4
Intensity Change (Log2) 0.03 -0.0 -0.23 -0.41 -0.34 -0.39 -0.48 -0.48 -0.55 0.44 0.42 0.41 0.2 0.43 0.52 0.38 0.35 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.9 -4.5 -7.6 -8.0 -8.7 -8.5 -9.9 -10.1 -8.5 2.5 4.0 4.5 0.6 1.8 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 -2.5 -3.6 -2.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.7 2.0 1.8 1.9 1.4 0.5 1.0 1.4 0 0 0 0 0.8 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.5275 5.6682 5.1094 4.9181 4.6497 5.3569 3.8765 4.8612 4.402 4.3678 3.7665 4.274 6.4399 8.1318 6.9501 6.4624 6.5505 7.0553
Actin 0.0294 0.0024 0.0379 0.0036 0.001 0.0027 0.0225 0.0035 0.0209 0.0266 0.0094 0.0033 0.0374 0.004 0.0167 0.0447 0.0115 0.0068
Bud 0.0014 0.007 0.0045 0.0017 0.0031 0.0018 0.0005 0.0006 0.0016 0.0008 0.0008 0.0015 0.0014 0.0011 0.0066 0.003 0.0018 0.0014
Bud Neck 0.0024 0.0006 0.002 0.0008 0.0029 0.0092 0.0015 0.0004 0.0009 0.0016 0.001 0.0038 0.0054 0.0014 0.0013 0.0019 0.0017 0.0082
Bud Periphery 0.0012 0.003 0.0025 0.0018 0.0031 0.0015 0.0005 0.0002 0.0017 0.0003 0.0004 0.0007 0.001 0.0003 0.003 0.0015 0.001 0.0007
Bud Site 0.0082 0.0082 0.0145 0.0008 0.0029 0.0015 0.0027 0.0036 0.0043 0.0007 0.0016 0.0005 0.0066 0.0176 0.0043 0.0036 0.0017 0.0009
Cell Periphery 0.0006 0.0005 0.0005 0.0002 0.0003 0.0002 0.0004 0.0001 0.0004 0.0001 0.0001 0.0001 0.0004 0.0003 0.0005 0.0002 0.0001 0.0001
Cytoplasm 0.539 0.8559 0.72 0.7998 0.7673 0.7207 0.7556 0.924 0.7964 0.7872 0.7312 0.8158 0.6616 0.8754 0.8084 0.7916 0.8063 0.7916
Cytoplasmic Foci 0.0852 0.0271 0.0514 0.0232 0.0474 0.0611 0.0534 0.016 0.0555 0.0397 0.0606 0.062 0.0778 0.0396 0.0573 0.0398 0.0589 0.0607
Eisosomes 0.0014 0 0.0003 0 0 0 0.0002 0 0.0002 0.0001 0 0 0.0003 0 0.0001 0.0002 0 0
Endoplasmic Reticulum 0.0248 0.0089 0.0179 0.0048 0.0087 0.014 0.0069 0.0039 0.006 0.0032 0.003 0.0102 0.0195 0.0036 0.007 0.0069 0.0076 0.0092
Endosome 0.1304 0.0358 0.059 0.0415 0.081 0.0929 0.0395 0.0136 0.0319 0.0609 0.0785 0.0591 0.0769 0.0191 0.033 0.0241 0.0665 0.0567
Golgi 0.0329 0.0047 0.0295 0.0129 0.0066 0.0116 0.0101 0.004 0.0207 0.0421 0.0169 0.0075 0.02 0.0085 0.0169 0.0161 0.0142 0.014
Lipid Particles 0.0287 0.0002 0.0053 0.0002 0.0021 0.0006 0.0128 0.0012 0.0078 0.0115 0.0269 0.0009 0.0077 0.0002 0.003 0.0019 0.0015 0.0034
Mitochondria 0.0233 0.0085 0.0232 0.0437 0.0155 0.0352 0.0169 0.0011 0.0124 0.0028 0.011 0.0031 0.0144 0.0018 0.0107 0.0355 0.0086 0.012
None 0.0123 0.0042 0.0037 0.0528 0.0031 0.0103 0.0294 0.0098 0.0249 0.0021 0.003 0.0072 0.031 0.0173 0.0091 0.0039 0.0024 0.0183
Nuclear Periphery 0.0178 0.0039 0.0041 0.0017 0.0068 0.0055 0.0061 0.0012 0.0012 0.0023 0.0011 0.0028 0.0052 0.0008 0.0019 0.0013 0.0026 0.0023
Nucleolus 0.0016 0.0002 0.0002 0.0001 0.0017 0.0003 0.0028 0 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0001
Nucleus 0.0194 0.0141 0.0099 0.0038 0.0135 0.0098 0.0137 0.0026 0.0019 0.0063 0.003 0.0085 0.0044 0.0027 0.0049 0.002 0.0038 0.0039
Peroxisomes 0.0117 0.0012 0.0066 0.0004 0.0016 0.0019 0.0139 0.0117 0.0082 0.008 0.0438 0.007 0.0186 0.0033 0.0106 0.0108 0.0055 0.0051
Punctate Nuclear 0.0133 0.0059 0.0014 0.0003 0.0009 0.0053 0.0035 0.0003 0.0007 0.0019 0.0008 0.0012 0.0038 0.0005 0.0011 0.0003 0.0014 0.0012
Vacuole 0.0115 0.0065 0.0043 0.0037 0.0229 0.0098 0.0052 0.0018 0.0017 0.0012 0.0055 0.0035 0.0049 0.002 0.0026 0.0083 0.002 0.0021
Vacuole Periphery 0.0035 0.0013 0.0013 0.0021 0.0077 0.004 0.0019 0.0003 0.0007 0.0005 0.0012 0.0012 0.0014 0.0003 0.0008 0.0024 0.0009 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.9881 22.1577 24.9282 25.4293 24.3619 33.5633 33.9668 34.1441 44.812 30.734
Translational Efficiency 1.2981 0.9762 0.9959 1.2441 0.9998 0.8926 0.8521 1.0669 0.9004 0.904

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
Localization
Cell Percentages cytoplasm (42%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Xdj1

Xdj1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Xdj1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available