Standard name
Human Ortholog
Description Aspartic protease; hyperglycosylated member of the yapsin family of proteases, attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor; involved in nutrient limitation-induced cleavage of the extracellular inhibitory domain of signaling mucin Msb2p, resulting in activation of the filamentous growth MAPK pathway; involved with other yapsins in the cell wall integrity response; role in KEX2-independent processing of the alpha factor precursor

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.1 0 0 0.05 0 0 0 0 0 0.08 0.1 0.06 0.12 0.13 0.17 0 0 0 0 0 0
Cytoplasm 0.87 0.82 0.92 0.9 0.81 0.71 0.67 0.79 0.77 0.63 0.73 0.72 0.68 0.9 0.89 0.9 0.82 0.39 0.4 0.41 0.32 0.09 0.18
Endoplasmic Reticulum 0.08 0.14 0 0.07 0.09 0.16 0.23 0 0.08 0.13 0.12 0.32 0.32 0.07 0 0 0.05 0.34 0.37 0.22 0.34 0.7 0.54
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.05 0.11 0.09 0.24 0.11 0 0 0 0 0 0 0.1 0.06 0.17 0.09 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.09 0.08 0.05 0.05 0.06 0 0 0 0 0.05 0 0 0 0.05 0 0.05 0.05 0 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 3 4 5 11 15 20 4 6 6 7 0 0 0 0 3 3 10 11 9 1 2 5
Bud 1 2 0 5 4 5 7 9 9 5 4 0 0 0 0 1 2 5 3 2 1 4 5
Bud Neck 0 0 10 1 0 0 0 0 3 1 2 0 0 0 0 1 1 6 6 10 1 0 1
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Cell Periphery 4 4 26 8 13 22 22 11 10 9 13 2 15 10 10 12 19 7 3 8 0 2 3
Cytoplasm 285 206 249 274 303 320 372 239 263 210 244 18 102 152 75 83 94 146 116 127 15 6 20
Endoplasmic Reticulum 26 35 9 20 32 70 127 12 29 42 41 8 48 11 3 4 6 128 107 69 16 54 63
Endosome 0 3 1 0 2 2 5 0 3 0 1 0 1 1 1 1 1 2 2 3 1 0 0
Golgi 1 2 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 1 1 0 0 0 0
Mitochondria 9 3 0 2 3 27 27 34 30 79 37 0 0 0 1 0 1 37 18 53 4 1 4
Nucleus 1 1 0 3 3 4 6 3 3 3 4 2 1 0 0 0 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 1 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0
SpindlePole 3 0 0 0 3 0 1 2 0 1 3 0 1 0 0 0 1 2 0 1 0 1 0
Vac/Vac Membrane 5 9 5 6 34 35 27 14 21 6 13 0 0 8 3 2 4 18 12 17 2 2 9
Unique Cell Count 327 252 271 304 373 451 553 301 342 332 334 25 149 169 84 92 115 375 292 310 50 78 118
Labelled Cell Count 337 268 304 324 408 500 616 328 378 363 369 30 169 182 93 107 134 375 292 310 50 78 118


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.7 6.1 6.1 7.1 6.0 6.1 5.4 5.4 4.6 5.1 8.0 7.6 7.0 8.3 8.4 8.1 10.0 10.7
Std Deviation (1e-4) 0.8 1.0 0.9 1.6 1.8 1.2 1.1 1.4 1.0 0.8 1.0 1.9 1.4 1.6 2.0 2.6 2.4 3.0 2.7
Intensity Change (Log2) 0.02 0.23 -0.0 0.01 -0.18 -0.18 -0.39 -0.25 0.39 0.33 0.2 0.45 0.47 0.42 0.72 0.82


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 1.2 1.5 1.7 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck -2.9 0 0 0 0 -2.4 0 -2.7 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -3.5 -3.2 -2.5 -3.2 -2.9 -3.5 0 -2.8 0 0.2 -1.4 0.6 0.9 1.9
Cytoplasm -0.7 -3.8 -6.7 -7.7 -4.2 -5.0 -8.2 -5.9 -3.2 -6.2 -0.7 -0.7 -0.5 -2.9
Endoplasmic Reticulum 1.8 2.7 5.1 7.1 0.4 2.6 0 4.0 0 8.3 1.6 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.1 3.7 5.7 5.0 0 5.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.1 3.8 3.4 2.1 1.9 2.6 0 1.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2053 2.1984 1.8673 1.413 1.3432 1.7874 1.328 1.9047 2.177 1.6283 1.2501 1.6439 1.2258 2.0871 2.4209 1.4743 1.5451 1.7103
Actin 0.0075 0.0064 0.0028 0.0033 0.0005 0.0022 0.1005 0.0009 0.0333 0.0901 0.0005 0.0132 0.0094 0.0016 0.026 0.0253 0.0501 0.0254
Bud 0.0004 0.0009 0.0003 0.0005 0.0001 0.0001 0.0013 0.0012 0.0003 0.0006 0.0001 0.0017 0.0007 0.001 0.0042 0.0019 0.0007 0.002
Bud Neck 0.0049 0.0007 0.0011 0.0019 0.0007 0.0013 0.0026 0.0003 0.0022 0.0102 0.0005 0.0117 0.0018 0.001 0.0018 0.0014 0.0016 0.0023
Bud Periphery 0.0006 0.0012 0.0005 0.0009 0.0002 0.0003 0.0031 0.0059 0.0007 0.0013 0.0003 0.004 0.0015 0.0021 0.004 0.0025 0.0012 0.0043
Bud Site 0.007 0.0165 0.0038 0.001 0.0008 0.0002 0.0148 0.0053 0.0013 0.0067 0.0002 0.002 0.0033 0.0235 0.0152 0.0047 0.0044 0.0011
Cell Periphery 0.0009 0.0033 0.0014 0.001 0.0002 0.0005 0.0023 0.0044 0.0023 0.0033 0.0008 0.0024 0.0017 0.0033 0.0014 0.0017 0.0004 0.0006
Cytoplasm 0.3173 0.4665 0.4388 0.3597 0.4913 0.5277 0.3099 0.4339 0.4223 0.3983 0.4687 0.4849 0.3931 0.5311 0.4015 0.4921 0.5397 0.5221
Cytoplasmic Foci 0.0391 0.0251 0.0296 0.0139 0.017 0.0192 0.0364 0.0098 0.0101 0.0126 0.0093 0.0132 0.021 0.0073 0.0243 0.0154 0.025 0.017
Eisosomes 0.0004 0.0002 0.0002 0.0001 0 0.0002 0.0009 0.0002 0.0004 0.0017 0.0001 0.0006 0.0003 0.0001 0.0002 0.0006 0.0002 0.0001
Endoplasmic Reticulum 0.2248 0.2176 0.2271 0.2125 0.2265 0.1691 0.1372 0.2052 0.2294 0.1442 0.1439 0.1009 0.2475 0.2216 0.2102 0.1929 0.1342 0.2119
Endosome 0.046 0.0238 0.0474 0.0612 0.0671 0.0267 0.015 0.015 0.0218 0.0252 0.0567 0.0076 0.02 0.0066 0.0357 0.0346 0.0656 0.014
Golgi 0.009 0.0046 0.0099 0.0235 0.0114 0.0031 0.0061 0.003 0.0071 0.0085 0.016 0.0019 0.0034 0.0013 0.0087 0.0172 0.0179 0.0051
Lipid Particles 0.0327 0.0101 0.0203 0.0295 0.0247 0.0182 0.0083 0.005 0.0069 0.008 0.0225 0.0033 0.0144 0.0067 0.0122 0.0353 0.0301 0.0084
Mitochondria 0.0075 0.0072 0.0132 0.0244 0.0017 0.0024 0.0021 0.0008 0.0018 0.014 0.0025 0.0015 0.0031 0.0042 0.0098 0.0179 0.0062 0.0211
None 0.2497 0.1907 0.1715 0.2199 0.1183 0.201 0.3197 0.2887 0.2433 0.2449 0.2604 0.3346 0.2274 0.1649 0.2174 0.1294 0.083 0.1295
Nuclear Periphery 0.02 0.007 0.0086 0.0192 0.0147 0.0096 0.0064 0.0072 0.0059 0.0074 0.0052 0.0034 0.0234 0.0057 0.0065 0.0086 0.0056 0.007
Nucleolus 0.0013 0.0005 0.0003 0.0005 0.0036 0.0018 0.0007 0.0002 0.0003 0.0008 0.0002 0.0009 0.001 0.0003 0.0007 0.0009 0.0005 0.0003
Nucleus 0.0038 0.0043 0.0033 0.008 0.0055 0.0031 0.0032 0.0028 0.0035 0.0031 0.0026 0.003 0.0089 0.0048 0.0032 0.0033 0.0024 0.0048
Peroxisomes 0.0053 0.0019 0.0103 0.0026 0.0023 0.0017 0.0164 0.0002 0.001 0.0077 0.0004 0.0012 0.004 0.0034 0.0036 0.0032 0.0221 0.0076
Punctate Nuclear 0.0114 0.0022 0.002 0.0016 0.003 0.0014 0.0089 0.0006 0.0012 0.0036 0.0005 0.0031 0.0057 0.0017 0.0037 0.0015 0.0044 0.0084
Vacuole 0.0078 0.0088 0.0065 0.0121 0.0087 0.0077 0.0038 0.0085 0.0042 0.0069 0.007 0.0042 0.007 0.0075 0.0076 0.0084 0.0036 0.0059
Vacuole Periphery 0.0025 0.0005 0.0012 0.0026 0.0016 0.0027 0.0005 0.0009 0.0006 0.0011 0.0016 0.0006 0.0016 0.0003 0.0018 0.0011 0.001 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 94.2088 41.5316 35.0986 46.725 42.6674 84.483 58.1772 54.9129 63.5953 67.7191
Translational Efficiency 0.9298 0.9531 0.8734 1.0221 1.1566 1.5182 0.9964 1.0143 0.9653 0.9982

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Aspartic protease; hyperglycosylated member of the yapsin family of proteases, attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor; involved in nutrient limitation-induced cleavage of the extracellular inhibitory domain of signaling mucin Msb2p, resulting in activation of the filamentous growth MAPK pathway; involved with other yapsins in the cell wall integrity response; role in KEX2-independent processing of the alpha factor precursor
Localization
Cell Percentages ER (52%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Yps1

Yps1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yps1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available