Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; has an extraribosomal function in autoregulation, in which Rps28Bp binds to a decapping complex via Edc3p, which then binds to RPS28B mRNA leading to its decapping and degradation; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0.06 0.09 0.07 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.93 0.94 0.92 0.95 0.88 0.77 – 0.53 0.55 0.47 0.46 0.43 0.96 0.96 0.96 – – – 0.94 0.89 0.89 0.86 0.87 0.86
Endoplasmic Reticulum 0 0 0 0 0.09 0.09 – 0.12 0.11 0.1 0.1 0.12 0 0 0 – – – 0 0 0 0 0.08 0.05
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0.05 0.12 0.13 0 0.2 – 0.28 0.46 0.47 0.56 0.59 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0.15 0 0.08 0 0 0 0 0 – – – 0 0 0 0.07 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 1 2 2 – 10 4 5 9 7 0 0 1 – – – 1 0 2 0 0 1
Bud 1 0 4 0 1 1 – 2 0 2 2 0 0 0 0 – – – 1 0 0 0 0 4
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 5 0 2 1 12 9 – 14 12 23 23 21 6 8 12 – – – 0 0 2 0 0 0
Cytoplasm 169 62 199 235 247 298 – 280 185 168 117 129 326 335 435 – – – 173 76 210 136 243 264
Endoplasmic Reticulum 6 1 5 3 25 35 – 62 38 35 26 36 13 9 13 – – – 2 2 10 4 23 14
Endosome 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 – – – 2 0 0 0 0 2
Golgi 0 0 0 1 0 2 – 10 2 5 0 4 1 1 0 – – – 1 2 0 1 1 2
Mitochondria 7 3 25 31 9 78 – 148 155 167 143 177 1 2 0 – – – 1 0 7 1 2 4
Nucleus 0 1 0 1 0 0 – 1 0 2 0 1 4 0 3 – – – 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 2 – 4 4 3 2 6 0 0 0 – – – 0 0 0 0 1 2
Nucleolus 0 0 0 0 0 0 – 0 1 1 0 0 0 0 1 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 2 4 – 78 9 27 7 11 1 2 0 – – – 0 1 1 10 6 7
Unique Cell Count 181 66 216 247 282 389 524 334 355 255 302 340 350 452 185 85 237 159 281 306
Labelled Cell Count 188 67 237 273 298 431 610 410 438 329 392 352 357 466 185 85 237 159 281 306


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.0 12.4 8.8 14.0 16.7 13.4 – 12.0 10.0 10.3 8.9 8.9 15.6 16.0 16.6 – – – 14.9 16.0 15.5
Std Deviation (1e-4) 2.8 3.1 1.9 2.7 2.8 3.1 – 2.8 2.5 2.7 2.3 2.3 2.8 3.1 2.9 – – – 2.5 3.8 3.5
Intensity Change (Log2) – – – 0.67 0.93 0.6 – 0.45 0.19 0.22 0.01 0.02 0.82 0.86 0.92 – – – 0.76 0.87 0.82


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 2.2 0 – 0 1.9 3.1 3.9 3.3 0 0 0 – – –
Cytoplasm 1.3 -1.6 -4.8 – -10.0 -9.2 -10.8 -10.6 -11.5 1.9 1.8 2.3 – – –
Endoplasmic Reticulum 0 3.0 3.2 – 4.1 3.9 3.4 3.4 4.0 1.0 0.2 0.4 – – –
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Mitochondria 0.3 -3.7 2.7 – 4.9 8.5 8.7 10.0 10.8 -6.1 -6.0 -7.4 – – –
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0 0 – 5.8 0 3.8 0 2.4 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.283 34.9861 31.9318 29.3516 23.8579 34.6195 23.8053 36.028 31.7537 20.1253 31.0034 30.9955 32.8186 43.0172 40.1459 35.3101 25.321 39.5378
Actin 0.0105 0.0009 0.0055 0.0011 0.0083 0.0022 0.0046 0.0013 0.0018 0.0061 0.0003 0.0059 0.0078 0.0003 0.0101 0.0691 0.0151 0.0012
Bud 0.0025 0.0017 0.0012 0.0032 0.0007 0.0015 0.0017 0.0075 0.0025 0.007 0.0002 0.002 0.0017 0.0058 0.001 0.0005 0.0019 0.0007
Bud Neck 0.0008 0.0005 0.0006 0.0016 0.0006 0.0012 0.0004 0.0005 0.0006 0.0006 0.0002 0.0014 0.0011 0.0003 0.0005 0.0005 0.0186 0.0015
Bud Periphery 0.0027 0.0005 0.0006 0.0021 0.0004 0.0018 0.0015 0.0104 0.0021 0.0105 0 0.0017 0.0015 0.0026 0.0005 0.0002 0.0016 0.0002
Bud Site 0.0025 0.0016 0.001 0.0029 0.0006 0.0001 0.001 0.0011 0.0008 0.0041 0 0.0002 0.0061 0.0011 0.002 0.0004 0.0052 0.0001
Cell Periphery 0.002 0.0013 0.0008 0.0006 0.0003 0.0012 0.0009 0.0017 0.0006 0.0014 0 0.0007 0.0018 0.0003 0.0005 0.0002 0.0003 0.0002
Cytoplasm 0.6975 0.9456 0.8193 0.701 0.7229 0.7975 0.7339 0.9267 0.8864 0.4754 0.9407 0.7626 0.7935 0.9493 0.8684 0.6707 0.5986 0.8698
Cytoplasmic Foci 0.0338 0.0053 0.0159 0.0232 0.0423 0.0168 0.0257 0.0059 0.0178 0.024 0.0043 0.02 0.0146 0.0037 0.0102 0.021 0.0672 0.0159
Eisosomes 0.0002 0 0.0001 0 0.0001 0.0001 0.0002 0 0 0 0 0 0.0002 0 0.0002 0.0004 0.0003 0
Endoplasmic Reticulum 0.0403 0.0083 0.0285 0.018 0.0239 0.0438 0.0506 0.0103 0.0061 0.0039 0.0045 0.06 0.0464 0.0172 0.0094 0.0488 0.0185 0.0365
Endosome 0.059 0.0103 0.0292 0.0977 0.0883 0.0691 0.0433 0.0127 0.0185 0.1113 0.02 0.0642 0.0387 0.0062 0.0252 0.035 0.1165 0.0468
Golgi 0.0124 0.0019 0.0032 0.0058 0.0115 0.0205 0.0085 0.0036 0.0093 0.1147 0.0009 0.0314 0.0059 0.0009 0.0052 0.0144 0.0159 0.0088
Lipid Particles 0.0063 0.0005 0.005 0.0027 0.0254 0.0001 0.0271 0.0002 0.0155 0.0014 0 0.0001 0.0052 0 0.0008 0.0042 0.0225 0.0002
Mitochondria 0.019 0.0013 0.006 0.004 0.0143 0.0258 0.0076 0.006 0.002 0.0189 0.0002 0.0332 0.01 0.0013 0.0151 0.0018 0.0145 0.0032
None 0.0072 0.0004 0.0039 0.0044 0.0069 0.0009 0.0278 0.0003 0.0096 0.0005 0.0002 0.0009 0.0072 0.0007 0.0113 0.0003 0.011 0.0004
Nuclear Periphery 0.0106 0.0009 0.0041 0.0071 0.0066 0.0015 0.0122 0.0008 0.0018 0.0058 0.0073 0.0019 0.0145 0.0009 0.0026 0.007 0.022 0.0019
Nucleolus 0.0016 0.0002 0.0006 0.0012 0.0002 0 0.0002 0 0.0001 0.0004 0 0 0.0003 0 0.0001 0 0.0089 0
Nucleus 0.0059 0.0011 0.0037 0.0091 0.0021 0.0008 0.0024 0.0008 0.0012 0.0038 0.0033 0.0008 0.0054 0.0007 0.0032 0.0011 0.0112 0.0008
Peroxisomes 0.003 0.0001 0.0028 0.0017 0.0061 0.0001 0.0045 0.0001 0.0047 0.0022 0 0.0002 0.0018 0 0.001 0.0229 0.0051 0.0001
Punctate Nuclear 0.0088 0 0.01 0.0055 0.0035 0 0.0013 0 0.0004 0.0002 0.0001 0.0001 0.0023 0 0.0012 0.0003 0.02 0.0001
Vacuole 0.0579 0.0148 0.0452 0.0803 0.0259 0.0094 0.0356 0.0078 0.015 0.1691 0.0137 0.0066 0.0274 0.0077 0.0258 0.0793 0.0165 0.0082
Vacuole Periphery 0.0155 0.0028 0.0127 0.0266 0.0091 0.0056 0.0091 0.0022 0.0032 0.0387 0.004 0.006 0.0064 0.0009 0.0056 0.0221 0.0085 0.0034

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 819.4401 1179.7833 1445.9278 882.2186 1146.5935 902.7771 773.6594 760.5669 945.5893 781.0162
Translational Efficiency 1.9304 1.6751 1.3646 2.5417 2.096 1.922 2.2093 2.1001 1.7398 2.203

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; has an extraribosomal function in autoregulation, in which Rps28Bp binds to a decapping complex via Edc3p, which then binds to RPS28B mRNA leading to its decapping and degradation; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Rps28b

Rps28b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps28b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available