ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.72 0.62 0.79 0.83 0.6 0.59 0.56 0.49 0.58 0.62 0.63 0.61 0.77 0.81 0.88 0.14 0.13 0.16 0.29 0.13 0.2 0.16 0.22 0.21
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0 0 0 0 0 0
Endosome 0 0 0 0 0.08 0 0 0 0 0 0 0 0.07 0.09 0.05 0 0.09 0 0 0 0 0.07 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0.08 0 0.15 0.23 0.07 0.17 0.19 0.16 0.22 0.17 0.17 0.2 0 0 0 0 0.07 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.05 0.14 0.15 0.13 0.1 0.1 0.07 0 0 0 0.21 0.17 0.26 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.25 0.42 0.1 0.14 0.44 0.51 0.4 0.46 0.41 0.36 0.35 0.38 0.2 0.15 0.11 0.68 0.71 0.66 0.58 0.78 0.69 0.64 0.61 0.6
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1
Bud 0 2 1 0 1 1 0 1 1 3 1 4 3 1 1 0 0 0 6 7 0 2 9 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 231 239 41 122 107 114 193 191 147 201 178 235 208 304 342 20 20 18 104 52 16 17 64 66
Endoplasmic Reticulum 2 3 0 0 0 0 1 1 0 0 0 4 0 2 1 3 9 11 1 1 0 0 2 2
Endosome 3 5 0 0 14 1 2 1 0 2 0 0 19 35 18 3 14 3 13 11 0 7 7 15
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 1 1 1 2 4 6 2 1 0 1 1 3
Mitochondria 25 17 8 34 12 32 66 62 56 56 49 78 4 2 2 4 11 2 10 7 2 4 9 10
Nucleus 2 17 1 0 1 10 47 57 32 32 28 26 1 3 1 29 27 29 1 2 0 1 3 4
Nuclear Periphery 1 3 0 1 0 0 1 5 5 3 4 11 0 0 0 0 4 0 0 0 0 0 0 0
Nucleolus 0 0 1 1 0 0 0 0 0 2 3 1 0 0 0 1 1 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 2 0 0 1 0 0 0 0 1 0 0 1 4 2 0 1 0 3 2 0 2 4 4
Vac/Vac Membrane 79 161 5 20 78 98 138 179 104 116 98 147 54 55 43 94 111 75 205 316 55 70 176 192
Unique Cell Count 321 386 52 147 177 193 343 386 253 326 282 385 270 376 390 139 156 113 357 408 81 111 289 319
Labelled Cell Count 344 450 57 178 215 256 449 498 345 416 361 506 291 409 412 156 202 145 357 408 81 111 289 319


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.6 4.0 3.6 5.1 4.4 4.2 4.5 4.1 4.6 4.6 4.7 4.7 4.9 4.9 10.5 9.5 9.0 4.3 5.4 5.4
Std Deviation (1e-4) 0.7 0.8 2.4 2.0 3.1 2.4 1.5 1.5 1.4 1.7 1.8 2.2 1.7 1.8 2.0 3.4 2.8 2.7 1.1 2.7 2.4
Intensity Change (Log2) – – – -0.15 0.36 0.15 0.07 0.17 0.04 0.21 0.2 0.23 0.25 0.3 0.29 1.41 1.26 1.18 0.1 0.43 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 7.1 7.7 6.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7242 0.1166 -0.1216 -0.1789 -0.4124 0.2827 -1.3587 -0.6916 -0.9631 -0.888 -0.988 -0.7228 1.0133 1.236 0.8849 1.0251 0.9458 0.9445
Actin 0.0059 0.0002 0.0267 0.0025 0.0091 0.0066 0.0251 0.0003 0.0011 0.0297 0.0004 0.0028 0.05 0.0001 0.0127 0.0213 0.008 0
Bud 0.0004 0.0003 0.0009 0.0004 0.0008 0.0005 0.0007 0.0023 0.0006 0.01 0.0003 0.0007 0.0015 0.0007 0.0009 0.0007 0.0005 0.0002
Bud Neck 0.0005 0.0009 0.0007 0.0072 0.0017 0.0017 0.0007 0.0049 0.0007 0.008 0.0005 0.0019 0.0153 0.0005 0.0031 0.0004 0.0006 0.0023
Bud Periphery 0.0012 0.0003 0.0013 0.0005 0.0012 0.0008 0.001 0.0031 0.0012 0.0362 0.0004 0.0011 0.0021 0.0007 0.0018 0.0011 0.0008 0.0004
Bud Site 0.0022 0.0014 0.008 0.0013 0.0037 0.0008 0.0047 0.0207 0.0024 0.0096 0.0008 0.0018 0.0143 0.0088 0.0099 0.0011 0.0026 0.0009
Cell Periphery 0.0008 0.0006 0.0006 0.0003 0.0011 0.0006 0.0005 0.001 0.0004 0.0017 0.0002 0.0004 0.0011 0.0007 0.0006 0.0002 0.0009 0.0014
Cytoplasm 0.1054 0.1289 0.1093 0.13 0.0512 0.1165 0.0923 0.1525 0.1181 0.1026 0.0959 0.1746 0.1185 0.1639 0.1185 0.0942 0.1371 0.0873
Cytoplasmic Foci 0.067 0.0749 0.052 0.0735 0.1204 0.0626 0.0665 0.0667 0.06 0.0581 0.0685 0.103 0.0684 0.0449 0.0593 0.0368 0.0551 0.0489
Eisosomes 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0003 0.0002 0 0.0001 0.0006 0.0001 0.0001 0.0002 0.0002 0
Endoplasmic Reticulum 0.0084 0.0057 0.0078 0.0028 0.0023 0.0035 0.0039 0.0033 0.0043 0.0033 0.0022 0.0042 0.008 0.0067 0.0053 0.0018 0.0025 0.003
Endosome 0.1651 0.1668 0.1986 0.1917 0.3143 0.17 0.2377 0.1399 0.1765 0.2592 0.3524 0.2389 0.1989 0.1594 0.1717 0.2585 0.286 0.3222
Golgi 0.0055 0.0046 0.0085 0.0309 0.0106 0.0112 0.0125 0.0019 0.0056 0.0146 0.0102 0.0053 0.0076 0.0018 0.0061 0.0058 0.0048 0.0038
Lipid Particles 0.0236 0.0135 0.0166 0.0292 0.0525 0.0096 0.0196 0.0092 0.0141 0.0155 0.0041 0.0125 0.0183 0.0098 0.0065 0.0081 0.0156 0.0337
Mitochondria 0.0217 0.0019 0.0206 0.024 0.0104 0.0191 0.017 0.0029 0.028 0.0204 0.0243 0.0039 0.0052 0.0023 0.0058 0.0164 0.0051 0.0011
None 0.421 0.389 0.3282 0.2668 0.0428 0.2742 0.3825 0.4035 0.4539 0.2782 0.2513 0.2672 0.3503 0.3583 0.3867 0.314 0.2376 0.1067
Nuclear Periphery 0.0058 0.0061 0.0136 0.0023 0.0086 0.0034 0.0052 0.004 0.0083 0.0032 0.0404 0.0035 0.0108 0.0059 0.0055 0.0026 0.0055 0.0021
Nucleolus 0.0022 0.0015 0.0012 0.0015 0.0027 0.0015 0.0017 0.0028 0.0022 0.0011 0.0008 0.0018 0.0015 0.0025 0.0012 0.0011 0.0028 0.0037
Nucleus 0.0036 0.0034 0.0036 0.0036 0.0105 0.0032 0.0068 0.0071 0.0057 0.0025 0.0039 0.0057 0.0059 0.0039 0.0052 0.0022 0.008 0.0044
Peroxisomes 0.0111 0.0013 0.0032 0.0366 0.0159 0.0021 0.0082 0.0016 0.0119 0.0025 0.0011 0.0037 0.0112 0.0011 0.0122 0.0059 0.0085 0.0024
Punctate Nuclear 0.0091 0.0014 0.001 0.0024 0.0214 0.0013 0.004 0.0021 0.0014 0.0006 0.001 0.0024 0.0049 0.0009 0.005 0.0007 0.02 0.0006
Vacuole 0.1261 0.1861 0.1744 0.1706 0.294 0.277 0.0988 0.1603 0.0914 0.1131 0.1279 0.1529 0.0935 0.2079 0.1601 0.1966 0.1739 0.3453
Vacuole Periphery 0.0132 0.0113 0.0229 0.022 0.0249 0.0336 0.0104 0.0099 0.0121 0.0295 0.0135 0.0115 0.0122 0.0192 0.0217 0.0303 0.0239 0.0297

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –
ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (40%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YLR297W

YLR297W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ylr297w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available