Standard name | |
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Human Ortholog | |
Description | Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 47 | 71 | 51 | 67 | 64 | 50 | 119 | 124 | 87 | 123 | 118 | 108 | 119 | 205 | 178 | 264 | 185 | 328 | 101 | 187 | 101 | 228 | 293 | 298 |
Bud | 4 | 15 | 8 | 10 | 29 | 18 | 36 | 32 | 18 | 32 | 26 | 25 | 10 | 16 | 23 | 67 | 50 | 92 | 4 | 14 | 6 | 2 | 6 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 3 | 2 | 1 | 0 | 1 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 6 | 1 | 2 | 2 | 4 | 1 | 5 | 0 | 0 | 0 | 19 | 7 | 22 | – | – | – | – | – | – |
Cell Periphery | 6 | 11 | 20 | 14 | 4 | 2 | 11 | 12 | 2 | 7 | 9 | 12 | 45 | 88 | 101 | 45 | 33 | 41 | 0 | 1 | 2 | 3 | 1 | 1 |
Cytoplasm | 3 | 7 | 3 | 6 | 18 | 13 | 8 | 14 | 11 | 10 | 7 | 10 | 12 | 15 | 18 | 40 | 18 | 37 | 0 | 0 | 1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 9 | 34 | 5 | 14 | 6 | 11 | 13 | 17 | 13 | 14 | 20 | 6 | 43 | 45 | 41 | 10 | 11 | 19 | 0 | 4 | 1 | 1 | 2 | 2 |
Endosome | 2 | 1 | 1 | 0 | 0 | 4 | 1 | 1 | 1 | 2 | 2 | 2 | 0 | 0 | 1 | 16 | 12 | 21 | 2 | 5 | 1 | 1 | 1 | 7 |
Golgi | 27 | 22 | 49 | 52 | 78 | 23 | 157 | 164 | 92 | 135 | 93 | 91 | 82 | 143 | 150 | 206 | 148 | 230 | 2 | 6 | 3 | 3 | 8 | 19 |
Mitochondria | 18 | 10 | 1 | 12 | 4 | 78 | 17 | 53 | 79 | 82 | 88 | 74 | 7 | 15 | 14 | 62 | 46 | 65 | 1 | 3 | 1 | 1 | 3 | 5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 5 | 2 | 0 | 1 | 5 | 11 | 5 | 8 | 8 | 9 | 5 | 6 | 0 | 0 | 2 | 4 | 0 | 7 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 2 | 4 | 0 | 4 | 1 | 6 | 2 | 6 | 6 | 8 | 0 | 6 | 7 | 19 | 20 | 35 | 2 | 0 | 1 | 1 | 7 | 11 |
SpindlePole | 0 | 0 | 0 | 0 | 6 | 4 | 4 | 8 | 0 | 7 | 2 | 3 | 0 | 1 | 0 | 12 | 6 | 13 | 0 | 0 | 0 | 0 | 0 | 3 |
Vac/Vac Membrane | 3 | 4 | 1 | 2 | 3 | 5 | 2 | 5 | 1 | 3 | 2 | 2 | 0 | 0 | 0 | 26 | 18 | 13 | 0 | 1 | 0 | 0 | 0 | 0 |
Unique Cell Count | 78 | 127 | 76 | 110 | 145 | 136 | 223 | 274 | 176 | 248 | 206 | 192 | 193 | 312 | 311 | 465 | 324 | 552 | 121 | 231 | 125 | 248 | 331 | 365 |
Labelled Cell Count | 124 | 179 | 141 | 183 | 217 | 233 | 375 | 448 | 318 | 436 | 380 | 352 | 318 | 535 | 535 | 791 | 557 | 924 | 121 | 231 | 125 | 248 | 331 | 365 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 22.0 | 28.5 | 25.2 | 22.7 | 24.4 | 15.4 | 22.3 | 21.5 | 19.6 | 20.0 | 19.8 | 19.7 | 27.8 | 29.1 | 30.1 | 12.7 | 12.4 | 12.5 | 25.1 | 26.4 | 28.4 |
Std Deviation (1e-4) | 7.5 | 8.2 | 6.9 | 6.0 | 6.6 | 5.5 | 5.7 | 6.4 | 5.7 | 6.2 | 6.5 | 6.3 | 5.8 | 7.3 | 9.0 | 4.3 | 4.8 | 3.2 | 6.4 | 6.8 | 7.0 |
Intensity Change (Log2) | – | – | – | -0.16 | -0.05 | -0.71 | -0.18 | -0.23 | -0.36 | -0.34 | -0.35 | -0.36 | 0.14 | 0.21 | 0.26 | -0.99 | -1.03 | -1.01 | -0.01 | 0.07 | 0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.9 | -3.2 | -4.2 | -2.1 | -3.4 | -2.6 | -2.7 | -1.5 | -1.6 | -0.8 | -0.2 | -1.6 | -1.7 | -1.6 | -1.3 |
Bud | -0.3 | 1.8 | 0.6 | 1.2 | 0.3 | -0.1 | 0.6 | 0.5 | 0.6 | -1.6 | 0 | -0.9 | 0.9 | 1.1 | 1.4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.4 | -5.3 | -5.7 | -5.3 | -5.9 | -6.5 | -6.5 | -5.4 | -4.6 | -0.5 | 0.3 | 1.0 | -4.1 | -3.7 | -5.2 |
Cytoplasm | 0 | 2.0 | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | 0 | 0 |
Endoplasmic Reticulum | 1.4 | 0 | 0.4 | 0 | 0 | 0.2 | 0 | 0.8 | 0 | 3.0 | 1.8 | 1.6 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -2.3 | -1.5 | -7.0 | 1.0 | -0.7 | -1.8 | -1.5 | -2.9 | -2.5 | -3.2 | -2.9 | -2.5 | -3.3 | -2.9 | -3.8 |
Mitochondria | 2.5 | 0 | 8.1 | 0 | 3.8 | 6.8 | 5.5 | 6.6 | 6.1 | 0 | 0 | 0 | 3.0 | 3.1 | 2.8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.8727 | 15.7594 | 14.3598 | 10.9379 | 12.0376 | 15.3392 | 22.0249 | 20.0899 | 20.5445 | 20.0019 | 17.524 | 21.0116 | 5.8932 | 23.8469 | 24.6855 | 24.5627 | 14.7282 | 19.8348 |
Actin | 0.6911 | 0.9382 | 0.8846 | 0.7751 | 0.7384 | 0.8835 | 0.7533 | 0.9153 | 0.9453 | 0.9926 | 0.8708 | 0.8961 | 0.2076 | 0.8312 | 0.9806 | 0.9623 | 0.4942 | 0.8359 |
Bud | 0.002 | 0.0005 | 0.0011 | 0.0007 | 0.0019 | 0.0007 | 0.0022 | 0.0009 | 0.0013 | 0.0001 | 0.0028 | 0.0004 | 0.0006 | 0.0004 | 0.0004 | 0.0005 | 0.0009 | 0.0117 |
Bud Neck | 0.0079 | 0.002 | 0.0033 | 0.0042 | 0.0007 | 0.012 | 0.0015 | 0.0027 | 0.0117 | 0.0035 | 0.0011 | 0.0045 | 0.0004 | 0.0073 | 0.0072 | 0.0159 | 0.001 | 0.0022 |
Bud Periphery | 0.003 | 0.0004 | 0.0013 | 0.0011 | 0.002 | 0.0015 | 0.0027 | 0.0012 | 0.0018 | 0.0001 | 0.0037 | 0.0004 | 0.0007 | 0.0003 | 0.0005 | 0.0007 | 0.0012 | 0.0123 |
Bud Site | 0.0236 | 0.0187 | 0.0088 | 0.0015 | 0.002 | 0.0016 | 0.0036 | 0.0421 | 0.0106 | 0.0001 | 0.0052 | 0.0007 | 0.0019 | 0.1003 | 0.0021 | 0.0009 | 0.0045 | 0.0065 |
Cell Periphery | 0.0012 | 0.0005 | 0.0004 | 0.0003 | 0.0002 | 0.0005 | 0.0005 | 0.001 | 0.0005 | 0 | 0.0003 | 0.0002 | 0.0004 | 0.0011 | 0.0002 | 0.0003 | 0.0007 | 0.0006 |
Cytoplasm | 0.0115 | 0.0015 | 0.0121 | 0.0026 | 0.002 | 0.0008 | 0.0028 | 0.0013 | 0.0054 | 0 | 0.001 | 0.0029 | 0.1143 | 0.0002 | 0 | 0 | 0.0508 | 0.008 |
Cytoplasmic Foci | 0.0221 | 0.0016 | 0.0103 | 0.0361 | 0.0069 | 0.0133 | 0.0066 | 0.001 | 0.0006 | 0.0001 | 0.0038 | 0.0029 | 0.0289 | 0.0105 | 0.0002 | 0.0001 | 0.0064 | 0.0223 |
Eisosomes | 0.025 | 0.0214 | 0.0043 | 0.0021 | 0.0181 | 0.0171 | 0.0102 | 0.0124 | 0.0035 | 0.0014 | 0.001 | 0.0103 | 0.0017 | 0.0153 | 0.0039 | 0.017 | 0.0003 | 0.0014 |
Endoplasmic Reticulum | 0.0057 | 0.0001 | 0.0025 | 0.012 | 0.0014 | 0.0005 | 0.0031 | 0.0002 | 0.0004 | 0 | 0.0012 | 0.0006 | 0.0309 | 0 | 0 | 0 | 0.0096 | 0.0019 |
Endosome | 0.0555 | 0.0009 | 0.0218 | 0.0461 | 0.0238 | 0.0123 | 0.0202 | 0.0004 | 0.001 | 0 | 0.0507 | 0.0173 | 0.1747 | 0.0002 | 0.0001 | 0 | 0.0993 | 0.0219 |
Golgi | 0.0379 | 0.0056 | 0.0151 | 0.0645 | 0.0536 | 0.0143 | 0.0541 | 0.0057 | 0.0032 | 0.0017 | 0.0451 | 0.0231 | 0.0092 | 0.0038 | 0.0043 | 0.0019 | 0.0064 | 0.033 |
Lipid Particles | 0.0134 | 0.0003 | 0.0039 | 0.0278 | 0.0043 | 0.019 | 0.0141 | 0.0006 | 0.0004 | 0 | 0.0007 | 0.0004 | 0.0187 | 0.0005 | 0.0001 | 0.0001 | 0.0048 | 0.0062 |
Mitochondria | 0.0368 | 0.0015 | 0.0147 | 0.008 | 0.0726 | 0.0089 | 0.055 | 0.0039 | 0.0012 | 0 | 0.0086 | 0.0007 | 0.0095 | 0.0004 | 0.0001 | 0.0001 | 0.0046 | 0.0103 |
None | 0.0121 | 0.0034 | 0.0015 | 0.0026 | 0.0301 | 0.0022 | 0.0019 | 0.0085 | 0.0006 | 0 | 0.0001 | 0.0002 | 0.0911 | 0 | 0 | 0 | 0.0355 | 0.0006 |
Nuclear Periphery | 0.0146 | 0.0001 | 0.0035 | 0.0047 | 0.016 | 0.0011 | 0.0095 | 0.0004 | 0.002 | 0 | 0.0005 | 0.0135 | 0.1527 | 0 | 0 | 0 | 0.0506 | 0.0011 |
Nucleolus | 0.0016 | 0.0011 | 0.0001 | 0.0001 | 0.0041 | 0.0006 | 0.0039 | 0.0005 | 0.0001 | 0 | 0 | 0.0016 | 0.004 | 0 | 0 | 0 | 0.0146 | 0.0002 |
Nucleus | 0.0076 | 0.0004 | 0.0006 | 0.0003 | 0.0053 | 0.0005 | 0.0426 | 0.0005 | 0.0092 | 0 | 0.0001 | 0.0163 | 0.0422 | 0 | 0 | 0 | 0.1282 | 0.0012 |
Peroxisomes | 0.0077 | 0.0007 | 0.0027 | 0.0072 | 0.0038 | 0.0057 | 0.0032 | 0.0007 | 0.0004 | 0.0001 | 0.0006 | 0.0002 | 0.002 | 0.0282 | 0.0001 | 0.0001 | 0.0008 | 0.0022 |
Punctate Nuclear | 0.0047 | 0.0009 | 0.0009 | 0.0015 | 0.009 | 0.0023 | 0.0029 | 0.0003 | 0.0005 | 0 | 0.0001 | 0.0019 | 0.0823 | 0.0001 | 0 | 0 | 0.0105 | 0.0007 |
Vacuole | 0.0092 | 0.0001 | 0.0036 | 0.0006 | 0.0017 | 0.0008 | 0.0028 | 0.0002 | 0.0001 | 0 | 0.001 | 0.004 | 0.0143 | 0 | 0 | 0 | 0.0636 | 0.016 |
Vacuole Periphery | 0.0058 | 0.0001 | 0.0027 | 0.0009 | 0.0021 | 0.0006 | 0.0033 | 0.0001 | 0.0001 | 0 | 0.0014 | 0.0017 | 0.0119 | 0 | 0 | 0 | 0.0114 | 0.0037 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 70.8891 | 38.5734 | 51.7942 | 64.2414 | 47.9535 | 55.4477 | 59.4308 | 57.6607 | 54.9624 | 72.7546 |
Translational Efficiency | 1.7317 | 2.3784 | 2.0768 | 1.3713 | 2.2491 | 2.9978 | 2.01 | 2.6916 | 3.0507 | 2.1866 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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