Standard name
Human Ortholog
Description Covalently linked cell wall glycoprotein; present in the inner layer of the cell wall
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.06 0.07 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0.21 0.18 0.07 0 0 0 0 0 0
Cytoplasm 0.37 0.27 0.5 0.4 0.41 0.39 0.42 0.39 0.36 0.38 0.31 0.33 0.48 0.49 0.5 0.55 0.46 0.6 0.2 0.13 0.28 0.29 0.29 0.31
Endoplasmic Reticulum 0.56 0.69 0.16 0.13 0.17 0.1 0.11 0.09 0.13 0.12 0.12 0.11 0.14 0.15 0.15 0 0.05 0.05 0.61 0.71 0.15 0.17 0.14 0.1
Endosome 0 0 0 0 0.06 0 0.07 0 0 0 0 0 0.05 0.05 0 0 0.09 0.06 0 0 0 0 0 0
Golgi 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.11 0.05 0 0 0 0 0 0
Mitochondria 0.05 0 0.24 0.4 0.26 0.52 0.3 0.38 0.45 0.42 0.51 0.47 0.3 0.24 0.23 0.06 0.08 0.09 0.08 0 0.37 0.31 0.25 0.28
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.05 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0.06 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0.06 0.06 0 0 0 0.05 0 0 0 0.07 0.07 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.08 0 0.05 0.06 0 0 0.05 0.05 0 0.06 0.05 0 0.08 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 6 7 3 3 10 2 7 8 6 10 11 3 1 4 3 3 4 5 3 9 7 9 14
Bud 2 5 1 2 3 3 5 12 18 17 17 22 3 3 3 1 1 2 9 32 10 17 23 22
Bud Neck 2 3 2 2 0 2 6 11 6 7 3 6 0 1 2 0 1 0 1 4 1 1 2 2
Bud Site 1 2 0 0 0 3 0 2 0 0 3 7 1 0 0 1 1 0 – – – – – –
Cell Periphery 9 34 16 8 8 4 5 6 14 12 21 24 23 17 31 45 25 10 2 4 3 3 3 5
Cytoplasm 110 118 167 93 97 113 171 168 196 191 184 229 262 278 308 117 65 83 59 60 97 89 103 114
Endoplasmic Reticulum 169 300 53 31 40 28 45 41 73 59 69 78 76 87 92 9 7 7 182 319 52 51 50 37
Endosome 1 2 12 9 14 4 30 17 15 12 9 21 28 29 26 6 13 8 3 2 8 4 13 12
Golgi 2 0 21 9 7 0 10 1 3 4 3 3 15 20 31 14 15 7 2 1 3 5 9 10
Mitochondria 16 5 79 92 60 150 120 165 246 212 299 328 162 137 140 12 11 12 22 9 128 95 88 100
Nucleus 1 1 1 0 0 3 5 6 1 5 3 4 0 0 0 1 2 4 0 0 0 0 0 1
Nuclear Periphery 1 0 1 0 3 7 10 8 14 21 18 14 4 2 3 0 0 0 0 1 0 1 4 4
Nucleolus 1 1 1 0 2 5 14 18 14 9 10 21 4 1 4 1 0 3 0 0 0 1 0 2
Peroxisomes 1 2 13 12 5 0 11 9 11 8 12 22 31 21 32 8 6 8 0 1 13 10 14 9
SpindlePole 3 10 4 3 7 2 23 26 18 16 22 35 11 13 11 14 10 6 3 3 15 7 12 13
Vac/Vac Membrane 8 3 9 3 18 9 22 27 16 19 32 33 17 34 29 9 11 6 2 6 0 3 10 7
Unique Cell Count 301 437 334 231 234 289 404 433 541 502 587 701 542 570 610 211 140 138 299 452 349 304 351 365
Labelled Cell Count 330 492 387 267 267 343 479 524 653 598 715 858 640 644 716 241 171 160 299 452 349 304 351 365


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 22.2 23.5 9.0 9.9 11.5 7.5 9.5 8.9 9.2 9.2 9.4 10.3 10.0 10.1 9.9 25.6 24.5 26.1 9.5 10.0 8.8
Std Deviation (1e-4) 14.7 11.1 7.3 8.2 10.2 6.6 8.7 7.4 9.2 9.0 9.6 10.3 9.8 9.6 9.0 21.8 20.4 21.7 9.0 12.2 8.7
Intensity Change (Log2) – – – 0.15 0.36 -0.26 0.08 -0.02 0.03 0.04 0.07 0.2 0.15 0.18 0.14 1.51 1.45 1.54 0.08 0.16 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 1.0 0 -0.5 -0.7 -1.0 -0.4 -0.6 0 0 0 0 0 0
Bud 0 0 0 0 2.6 3.0 3.0 2.7 2.9 0 0 0 0 0 0
Bud Neck 0 0 0 0 2.1 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.8 -0.8 -2.4 -2.9 -2.8 -1.7 -1.9 -0.9 -1.1 -0.4 -1.4 0.2 6.0 4.6 1.1
Cytoplasm -2.3 -2.0 -2.7 -2.1 -3.1 -4.0 -3.4 -5.6 -5.4 -0.5 -0.4 0.1 1.2 -0.7 2.0
Endoplasmic Reticulum -0.8 0.4 -2.3 -1.9 -2.7 -1.0 -1.7 -1.8 -2.1 -0.7 -0.2 -0.3 -4.2 -3.2 -3.2
Endosome 0.2 1.3 -1.7 2.2 0.2 -0.7 -1.0 -2.0 -0.5 1.1 1.0 0.5 -0.5 2.5 1.1
Golgi -1.2 -1.8 -4.3 -2.6 -5.0 -5.0 -4.6 -5.3 -5.9 -2.5 -1.9 -0.8 0.2 1.7 -0.5
Mitochondria 4.1 0.5 7.3 1.8 4.3 6.5 5.5 8.1 7.1 2.0 0.1 -0.2 -5.5 -4.0 -3.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 2.5 3.4 2.8 0 0 0 0 0 0 0
Nucleolus 0 0 0 3.0 3.4 2.5 0 0 2.8 0 0 0 0 0 0
Peroxisomes 0.7 -1.2 -3.4 -0.9 -1.5 -1.6 -2.1 -1.7 -0.6 1.2 -0.2 0.9 -0.1 0.2 0.9
SpindlePole 0 0 0 3.2 3.4 2.0 1.8 2.2 3.0 0.9 1.2 0.7 3.5 0 0
Vacuole 0 2.8 0.3 1.9 2.3 0.2 0.9 2.0 1.5 0.4 2.2 1.5 1.0 2.6 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 42.7644 44.9373 42.6056 38.957 42.6834 45.3977 52.7921 58.4762 58.9188 56.5211 54.8379 57.8957 64.9546 58.1705 62.0253 62.9681 55.8395 59.0733
Actin 0.01 0.0028 0.0104 0.0056 0.0301 0.0025 0.0229 0.0005 0.0007 0.0009 0.0002 0.0012 0.0005 0.0011 0.0001 0.0001 0.0001 0.0003
Bud 0.0007 0.0001 0.0009 0.0002 0.0003 0.0002 0.0005 0.0002 0 0.0001 0 0.0001 0.0001 0.0002 0 0 0 0
Bud Neck 0.0005 0.0005 0.0007 0.0015 0.0011 0.0014 0.0006 0.0003 0.0006 0.0005 0.0029 0.002 0.0004 0.0004 0.0003 0.0004 0.0003 0.0027
Bud Periphery 0.0009 0.0002 0.0022 0.0007 0.0005 0.0003 0.0006 0.0005 0.0001 0.0001 0.0004 0.0002 0.0005 0.0005 0.0002 0.0002 0 0.0001
Bud Site 0.0023 0.001 0.0029 0.0006 0.0007 0.0001 0.0021 0.0002 0.0001 0.0001 0 0 0 0.0038 0 0 0 0
Cell Periphery 0.0231 0.0405 0.0385 0.0217 0.01 0.0087 0.0177 0.0537 0.0426 0.033 0.017 0.0175 0.0239 0.0427 0.0476 0.0629 0.0097 0.0149
Cytoplasm 0.0424 0.0359 0.0186 0.0217 0.003 0.0209 0.0948 0.0337 0.0174 0.0116 0.0408 0.0095 0.0074 0.014 0.0105 0.0027 0.0027 0.0027
Cytoplasmic Foci 0.0121 0.0012 0.0078 0.0156 0.0031 0.0033 0.0027 0.0003 0.001 0.001 0.0002 0.0003 0.0001 0.0003 0.0002 0 0.0001 0.0001
Eisosomes 0.0003 0.0026 0.0007 0.0001 0.0002 0.0001 0.0011 0.002 0.0001 0.0002 0.0001 0.0002 0.0008 0.0006 0.0005 0.0185 0.0002 0.0003
Endoplasmic Reticulum 0.7623 0.85 0.817 0.7615 0.8241 0.8693 0.7468 0.845 0.88 0.8673 0.8849 0.9239 0.8527 0.8862 0.8926 0.8461 0.964 0.9489
Endosome 0.0253 0.0059 0.0088 0.0444 0.003 0.0084 0.0041 0.0004 0.0108 0.0291 0.0015 0.004 0.0006 0.0002 0.0006 0.0003 0.0003 0.0004
Golgi 0.0064 0.0017 0.0046 0.0473 0.0088 0.0166 0.0033 0.0011 0.0022 0.0036 0.0005 0.0042 0.0008 0.0006 0.0005 0.0022 0.0012 0.0013
Lipid Particles 0.0121 0.0207 0.0208 0.0435 0.0085 0.0175 0.0057 0.0258 0.0112 0.0122 0.0049 0.0039 0.0013 0.0167 0.0025 0.0054 0.0031 0.0039
Mitochondria 0.0057 0.0001 0.0149 0.001 0.0422 0.0106 0.003 0.0001 0.0003 0.0033 0.0037 0.0013 0.0001 0 0 0.0001 0 0
None 0.0055 0.0001 0.002 0.0003 0.0002 0.0002 0.0035 0.0005 0.0001 0.0003 0.0003 0.0001 0.0001 0.0005 0.0001 0.0002 0.0001 0
Nuclear Periphery 0.0614 0.0175 0.0256 0.0174 0.0523 0.0266 0.0637 0.0236 0.0252 0.0273 0.0229 0.0245 0.1005 0.0253 0.0356 0.0529 0.0151 0.0207
Nucleolus 0.0006 0.0002 0.002 0.0003 0.0001 0.0001 0.0025 0.0003 0 0 0.0001 0 0 0.0004 0 0.0001 0 0
Nucleus 0.0028 0.0005 0.0026 0.0022 0.0007 0.0012 0.0113 0.0008 0.0006 0.0007 0.0012 0.0006 0.002 0.0006 0.0006 0.0007 0.0003 0.0003
Peroxisomes 0.0013 0 0.0007 0.0004 0.0041 0.0006 0.0002 0 0 0 0 0 0 0 0 0 0 0
Punctate Nuclear 0.0032 0.0001 0.0013 0.0007 0.0002 0.0003 0.0034 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0002 0.0001 0 0 0
Vacuole 0.0167 0.0178 0.0147 0.0122 0.004 0.0074 0.0078 0.0105 0.0063 0.0069 0.0137 0.0055 0.0054 0.0051 0.0071 0.006 0.0024 0.0025
Vacuole Periphery 0.0043 0.0009 0.0024 0.0012 0.0029 0.0036 0.0018 0.0004 0.0005 0.0016 0.0046 0.0008 0.0027 0.0004 0.0008 0.0014 0.0004 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 318.5561 423.7153 404.2479 392.1695 299.2912 372.2121 510.1374 452.9756 393.7559 358.3566
Translational Efficiency 2.1365 1.9634 2.0855 1.4764 2.1609 2.4136 1.9416 2.2031 2.6361 2.2998

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Covalently linked cell wall glycoprotein; present in the inner layer of the cell wall
Localization
Cell Percentages ER (100%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Ccw14

Ccw14


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ccw14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available