Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud 0 0 0.06 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0.09 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.15 0.09 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0.06 0 0 0 0 0 0
Endosome 0.05 0 0 0 0.1 0 – 0 0 0 0 0 0.05 0.06 0.08 0.08 0.19 0.12 0.09 0 0.07 0.08 0.09 0.08
Golgi 0 0.07 0 0 0 0 – 0 0 0 0 0 0.16 0.13 0.15 0.19 0.22 0.15 0.05 0.14 0 0.07 0.12 0.09
Mitochondria 0.81 0.91 0.98 0.98 0.81 0.91 – 0.83 0.96 0.93 0.96 0.94 0.83 0.83 0.76 0.1 0.08 0.12 0.69 0.73 0.77 0.66 0.55 0.58
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.11 – 0.1 0.1 0.08 0.07 0.11 0.06 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.07 0.07 0.11 – 0.14 0.1 0.08 0.11 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0.06 0.09 0.05 0 0 0.1 0.06 0.08 0.07
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0.25 0.14 0.05 0 0.09 0.05 – 0 0.05 0 0 0 0.16 0.15 0.21 0.33 0.39 0.39 0.08 0 0 0.06 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 2 0 0 0 0 – 0 0 0 0 0 5 7 1 1 3 3 7 2 1 4 13 14
Bud 5 1 14 13 2 3 – 7 7 12 7 9 1 2 1 1 4 4 2 0 0 0 3 5
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 1 2
Bud Site 5 0 1 0 0 1 – 3 4 5 5 5 0 0 1 0 0 0 – – – – – –
Cell Periphery 11 5 4 6 2 2 – 3 1 1 3 5 10 4 6 3 7 4 1 0 0 0 0 1
Cytoplasm 4 1 2 6 10 8 – 20 5 2 3 3 8 6 21 7 7 6 1 0 0 0 2 0
Endoplasmic Reticulum 9 4 4 2 0 1 – 0 1 2 1 0 5 15 3 2 2 4 1 0 0 0 0 1
Endosome 26 0 1 1 26 3 – 14 1 4 1 0 16 19 28 4 15 8 48 2 14 15 28 31
Golgi 16 8 1 0 6 0 – 0 0 0 0 0 47 42 54 9 17 10 26 18 5 12 41 33
Mitochondria 416 108 213 276 217 310 – 468 329 396 322 366 245 276 269 5 6 8 386 94 167 120 186 222
Nucleus 6 1 1 0 2 0 – 4 0 4 3 3 0 0 1 2 1 2 3 0 0 1 0 1
Nuclear Periphery 17 4 9 5 11 36 – 56 35 33 25 43 17 12 4 0 0 0 7 1 0 0 4 3
Nucleolus 23 2 8 20 20 38 – 77 33 34 38 27 8 12 1 2 1 1 4 0 0 0 3 4
Peroxisomes 3 1 2 5 14 1 – 9 2 10 1 0 11 6 9 3 7 3 10 1 22 11 26 26
SpindlePole 4 0 3 2 4 4 – 12 6 7 8 5 1 2 5 3 2 5 5 0 0 0 6 7
Vac/Vac Membrane 129 17 10 4 24 16 – 16 17 8 6 4 46 50 75 16 31 26 42 5 1 11 17 22
Unique Cell Count 513 119 218 282 268 340 564 341 426 337 388 295 333 353 48 79 66 562 130 218 183 336 383
Labelled Cell Count 680 154 273 340 338 423 689 441 518 423 470 420 453 479 58 103 84 562 130 218 183 336 383


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 8.3 5.4 6.2 7.2 5.4 – 5.6 5.0 5.3 5.2 4.8 7.9 7.5 7.5 8.1 8.7 8.3 6.9 7.9 8.1
Std Deviation (1e-4) 1.1 1.7 1.2 1.5 2.6 1.6 – 1.6 1.5 1.3 1.6 1.3 1.2 1.3 1.6 1.9 2.1 2.0 1.4 1.6 1.7
Intensity Change (Log2) – – – 0.22 0.42 0.02 – 0.06 -0.11 -0.03 -0.04 -0.15 0.55 0.49 0.48 0.6 0.69 0.64 0.37 0.56 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud -0.9 -3.5 -3.7 – -4.0 -2.6 -2.2 -2.6 0 -4.0 -4.0 -4.5 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 1.1 0 0 0 0 0
Cytoplasm 0 2.0 0 – 2.0 0 0 0 0 0 0 3.0 0 0 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 1.7 0 0 0 0
Endosome 0 4.4 0 – 0 0 0 0 0 3.1 3.2 4.0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 5.9 5.2 5.8 0 0 0
Mitochondria 0.1 -5.7 -3.1 – -5.5 -0.8 -2.5 -1.3 0 -5.3 -5.4 -6.9 -14.2 -15.6 -14.7
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.6 0 2.7 – 2.6 2.6 1.8 1.6 0 0.8 -0.3 0 0 0 0
Nucleolus 1.7 1.8 3.1 – 4.0 2.7 2.1 3.2 0 -0.6 0 0 0 0 0
Peroxisomes 0 2.6 0 – 0 0 0 0 0 2.0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.1 1.9 0.1 – -1.2 0.2 -2.0 -1.9 -2.8 4.0 3.8 5.4 0 7.6 7.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.4179 4.3707 4.1941 3.9984 3.3876 4.2807 4.1336 4.0496 3.7677 3.5674 3.4209 3.7744 6.0082 5.7832 5.7331 5.7406 4.9797 5.7831
Actin 0.014 0.006 0.0023 0.0031 0.0133 0.0046 0.0427 0.0293 0.0291 0.0433 0.003 0.0104 0.0402 0.014 0.0096 0.001 0.0014 0.0012
Bud 0.0005 0.0096 0.0033 0.0008 0.0001 0.0001 0.001 0.0006 0.0006 0.0004 0.0007 0.001 0.0006 0.0004 0.0004 0 0.0069 0.0001
Bud Neck 0.0003 0.0006 0.0001 0.0004 0.0001 0.0001 0.0031 0.0004 0.0005 0.0007 0.0001 0.004 0.0057 0.0002 0.0001 0 0.0002 0.0004
Bud Periphery 0.0008 0.0027 0.0019 0.0021 0.0003 0.0004 0.002 0.0008 0.0009 0.0008 0.0014 0.0091 0.0015 0.0009 0.0007 0.0001 0.0066 0.0001
Bud Site 0.001 0.0143 0.0017 0.0007 0.0002 0.0002 0.0135 0.0045 0.0019 0.0005 0.0003 0.0007 0.0031 0.0026 0.0011 0 0.0051 0.0001
Cell Periphery 0.0003 0.0004 0.0002 0.0002 0.0001 0.0001 0.0007 0.0003 0.0004 0.0001 0.0001 0.0006 0.0004 0.0003 0.0001 0 0.0001 0.0001
Cytoplasm 0.0012 0.0004 0.0013 0.0007 0.0003 0.0004 0.008 0.0004 0.0015 0.0001 0.0005 0.0034 0.0003 0.0002 0.0001 0 0.0024 0.0008
Cytoplasmic Foci 0.0081 0.0093 0.0062 0.0225 0.0049 0.0047 0.0123 0.0103 0.0083 0.0011 0.0031 0.006 0.0074 0.0037 0.0031 0.0003 0.0281 0.0057
Eisosomes 0.0003 0.0002 0.0001 0.0001 0.0005 0.0001 0.0012 0.0003 0.0004 0.0002 0.0002 0.0003 0.0014 0.0002 0.0005 0 0.0001 0.0001
Endoplasmic Reticulum 0.0009 0.0001 0.0001 0.0002 0.0009 0.001 0.0122 0.0002 0.0009 0.0002 0.0002 0.0019 0.0015 0.0003 0.0001 0 0.0002 0.0002
Endosome 0.0224 0.0111 0.0112 0.0169 0.0186 0.0194 0.0493 0.0325 0.0187 0.0066 0.0232 0.0301 0.0302 0.0108 0.0112 0.0005 0.0409 0.014
Golgi 0.0391 0.0286 0.0258 0.0251 0.0473 0.0342 0.033 0.0605 0.0271 0.0103 0.0186 0.0516 0.0344 0.0238 0.0439 0.0017 0.0318 0.0212
Lipid Particles 0.0216 0.0026 0.0058 0.0128 0.0088 0.0178 0.0377 0.0062 0.0042 0.0006 0.0034 0.0027 0.0234 0.007 0.0033 0.0002 0.0028 0.0209
Mitochondria 0.8433 0.8856 0.9012 0.8937 0.8781 0.8975 0.7323 0.8186 0.8854 0.9307 0.9293 0.8444 0.8169 0.9163 0.9112 0.9917 0.8454 0.9217
None 0.0071 0.0001 0.0065 0.001 0.0004 0.0002 0.0019 0.0001 0.0004 0.0001 0.0001 0.0092 0.0008 0.0001 0.0001 0 0.0004 0.0008
Nuclear Periphery 0.0028 0.0001 0.0003 0.0002 0.0135 0.0012 0.0112 0.0001 0.0009 0.0001 0.0002 0.0024 0.0017 0.0006 0 0 0.0003 0.0002
Nucleolus 0.0009 0.0006 0.0008 0.0007 0.0001 0.0001 0.0005 0.0003 0.0002 0 0.0001 0.0024 0.0003 0.0003 0.0001 0 0.0002 0.0001
Nucleus 0.0005 0.0004 0.0005 0.0002 0.0003 0.0001 0.0012 0.0002 0.0005 0 0.0001 0.0029 0.0003 0.0002 0 0 0.0002 0.0001
Peroxisomes 0.0203 0.0233 0.0216 0.0146 0.0081 0.013 0.0214 0.0266 0.0098 0.0011 0.0091 0.0027 0.0217 0.0118 0.0122 0.0041 0.0218 0.0079
Punctate Nuclear 0.0035 0 0.0019 0.0008 0.0006 0.0001 0.0013 0 0.0013 0 0 0.0017 0.0003 0.0002 0 0 0.0007 0.0004
Vacuole 0.0045 0.0013 0.0024 0.0017 0.0006 0.0011 0.005 0.0039 0.0037 0.0008 0.0023 0.0039 0.0029 0.0028 0.0006 0 0.0026 0.0011
Vacuole Periphery 0.0068 0.0027 0.0048 0.0016 0.0029 0.0037 0.0088 0.004 0.0033 0.002 0.0041 0.0086 0.0051 0.0035 0.0016 0.0002 0.0018 0.0028

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 75.1144 64.8322 89.344 54.4523 57.0902 98.3713 72.5752 74.2651 103.1005 75.7379
Translational Efficiency 1.5119 1.7062 1.025 1.949 2.1275 1.2539 1.7086 2.0636 1.3819 1.3365

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit
Localization
Cell Percentages mitochondrion (97%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mrpl39

Mrpl39


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrpl39-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available