ORF | |
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Human Ortholog | |
Description | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 5 | 4 | 7 | 3 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 5 | 3 | 5 | 0 | 2 | 5 | 7 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 6 | 3 | 2 | 3 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 4 | 2 | 5 | 23 | 15 | 7 | 10 | 9 | 13 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 19 | 14 | 23 | 14 | 36 | 79 | 136 | 143 | 103 | 155 | 115 | 157 | 15 | 20 | 19 | 17 | 23 | 14 | 0 | 1 | 1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 1 | 3 | 4 | 4 | 13 | 27 | 48 | 28 | 34 | 33 | 36 | 6 | 2 | 0 | 3 | 9 | 2 | 0 | 0 | 0 | 1 | 2 | 1 |
Endosome | 1 | 6 | 13 | 13 | 17 | 5 | 22 | 13 | 3 | 7 | 3 | 1 | 6 | 12 | 5 | 2 | 1 | 1 | 8 | 14 | 20 | 8 | 18 | 25 |
Golgi | 4 | 18 | 10 | 15 | 9 | 2 | 9 | 9 | 3 | 8 | 1 | 4 | 14 | 7 | 5 | 20 | 21 | 16 | 0 | 0 | 8 | 1 | 2 | 6 |
Mitochondria | 9 | 12 | 54 | 29 | 55 | 116 | 127 | 129 | 128 | 136 | 163 | 163 | 0 | 6 | 11 | 7 | 3 | 3 | 1 | 2 | 10 | 5 | 4 | 9 |
Nucleus | 34 | 49 | 39 | 8 | 18 | 41 | 22 | 19 | 17 | 20 | 14 | 19 | 17 | 19 | 33 | 16 | 16 | 13 | 22 | 27 | 25 | 17 | 16 | 21 |
Nuclear Periphery | 22 | 21 | 84 | 111 | 41 | 55 | 94 | 68 | 46 | 50 | 36 | 34 | 36 | 65 | 42 | 34 | 28 | 13 | 9 | 11 | 58 | 19 | 13 | 16 |
Nucleolus | 38 | 37 | 40 | 16 | 16 | 6 | 12 | 12 | 1 | 7 | 1 | 4 | 25 | 30 | 19 | 14 | 15 | 17 | 10 | 11 | 7 | 13 | 10 | 17 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 2 | 1 | 1 |
Vac/Vac Membrane | 360 | 514 | 399 | 355 | 477 | 417 | 586 | 506 | 328 | 369 | 268 | 322 | 329 | 416 | 300 | 196 | 185 | 124 | 402 | 539 | 456 | 247 | 202 | 329 |
Unique Cell Count | 436 | 582 | 534 | 438 | 586 | 597 | 876 | 822 | 544 | 669 | 528 | 636 | 387 | 478 | 362 | 231 | 228 | 161 | 466 | 618 | 597 | 324 | 280 | 438 |
Labelled Cell Count | 488 | 672 | 665 | 569 | 677 | 745 | 1064 | 972 | 669 | 805 | 651 | 764 | 448 | 579 | 437 | 311 | 302 | 205 | 466 | 618 | 597 | 324 | 280 | 438 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 21.7 | 20.4 | 12.6 | 13.8 | 11.1 | 9.1 | 9.0 | 8.9 | 8.4 | 7.5 | 7.8 | 8.2 | 20.0 | 16.5 | 14.0 | 22.9 | 23.1 | 23.1 | 19.1 | 18.4 | 19.8 |
Std Deviation (1e-4) | 5.9 | 5.5 | 4.9 | 4.0 | 3.1 | 3.0 | 2.9 | 2.8 | 2.6 | 2.7 | 2.5 | 2.6 | 6.4 | 5.2 | 5.6 | 8.4 | 8.8 | 9.0 | 6.4 | 6.2 | 6.1 |
Intensity Change (Log2) | – | – | – | 0.14 | -0.18 | -0.47 | -0.47 | -0.5 | -0.59 | -0.75 | -0.69 | -0.61 | 0.67 | 0.39 | 0.16 | 0.86 | 0.88 | 0.88 | 0.6 | 0.55 | 0.66 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 3.8 | 3.1 | 0 | 0 | 0 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.9 | 1.4 | 5.2 | 6.5 | 7.2 | 7.5 | 9.2 | 8.5 | 9.6 | -0.3 | -0.1 | 0.7 | 1.7 | 3.1 | 2.2 |
Endoplasmic Reticulum | 0 | 0 | 2.3 | 3.2 | 5.0 | 4.5 | 4.5 | 5.1 | 4.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.5 | 0.5 | -2.1 | 0.1 | -1.1 | -2.6 | -1.9 | -2.5 | -3.6 | -0.9 | 0.1 | -1.1 | 0 | 0 | 0 |
Golgi | 1.5 | -0.4 | -2.5 | -1.3 | -1.2 | -2.0 | -1.0 | -2.7 | -1.9 | 1.6 | -0.5 | -0.6 | 4.4 | 4.7 | 4.7 |
Mitochondria | -1.9 | -0.4 | 4.4 | 2.4 | 2.9 | 5.9 | 4.8 | 8.4 | 6.8 | -6.4 | -6.0 | -4.0 | -3.3 | -4.2 | -3.3 |
Nucleus | -4.0 | -3.2 | -0.3 | -4.3 | -4.4 | -3.1 | -3.4 | -3.5 | -3.4 | -1.8 | -2.3 | 1.0 | -0.2 | -0.1 | 0.3 |
Nuclear Periphery | 3.7 | -4.6 | -3.3 | -2.7 | -4.3 | -3.7 | -4.5 | -4.6 | -5.9 | -2.9 | -1.0 | -1.7 | -0.4 | -1.2 | -2.5 |
Nucleolus | -2.6 | -3.7 | -5.5 | -5.9 | -5.7 | -6.3 | -5.7 | -6.2 | -6.1 | -0.6 | -0.8 | -1.3 | -0.7 | -0.4 | 1.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.4 | 2.7 | -1.8 | -3.1 | -5.0 | -5.1 | -7.0 | -8.1 | -8.4 | 3.8 | 4.9 | 2.9 | 3.1 | 1.9 | 0.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.6875 | 15.0232 | 13.733 | 11.1013 | 9.9027 | 13.281 | 14.3418 | 18.1927 | 16.1969 | 15.6396 | 14.9718 | 16.5158 | 13.2101 | 17.0882 | 15.4223 | 13.9441 | 13.9072 | 14.9784 |
Actin | 0.0064 | 0 | 0.0002 | 0.0005 | 0.0001 | 0 | 0.0089 | 0 | 0.0002 | 0 | 0 | 0.001 | 0.0029 | 0 | 0.0005 | 0.0001 | 0.0012 | 0.0002 |
Bud | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Bud Neck | 0.0002 | 0 | 0 | 0.0005 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Periphery | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0004 | 0 | 0.0013 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0 | 0.0003 | 0.0004 | 0.0001 | 0.0002 |
Bud Site | 0.0011 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cell Periphery | 0.0008 | 0.001 | 0.001 | 0.0004 | 0.0009 | 0.0004 | 0.0015 | 0.0005 | 0.0014 | 0.001 | 0.0003 | 0.0004 | 0.0008 | 0.0005 | 0.0012 | 0.0009 | 0.0006 | 0.0007 |
Cytoplasm | 0.0022 | 0.0004 | 0.0005 | 0.003 | 0.0008 | 0.0002 | 0.001 | 0 | 0.0012 | 0 | 0 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0135 | 0.0002 | 0.0032 | 0.011 | 0.0017 | 0.0072 | 0.0035 | 0 | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0033 | 0.0001 | 0.0009 | 0.0003 | 0.0043 | 0.0005 |
Eisosomes | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0014 | 0.0002 | 0.0003 | 0.0013 | 0.0007 | 0.0001 | 0.0008 | 0.0002 | 0.0014 | 0.0001 | 0.0002 | 0.0005 | 0.0006 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0001 |
Endosome | 0.0329 | 0.0028 | 0.0092 | 0.0565 | 0.0675 | 0.0108 | 0.0084 | 0.0012 | 0.0052 | 0.0033 | 0.0032 | 0.0033 | 0.0077 | 0.0019 | 0.0056 | 0.0061 | 0.0034 | 0.0021 |
Golgi | 0.0063 | 0.0001 | 0.0055 | 0.0234 | 0.0025 | 0.0009 | 0.0017 | 0 | 0.0003 | 0 | 0.0007 | 0.0005 | 0.0011 | 0.0001 | 0.0005 | 0.0004 | 0.0009 | 0.0003 |
Lipid Particles | 0.0162 | 0.0005 | 0.003 | 0.0046 | 0.0054 | 0.001 | 0.0059 | 0.0002 | 0.0008 | 0.0004 | 0.0015 | 0.0041 | 0.006 | 0.0036 | 0.003 | 0.005 | 0.0093 | 0.0017 |
Mitochondria | 0.0071 | 0.0028 | 0.011 | 0.0044 | 0.0366 | 0.0007 | 0.0105 | 0.0004 | 0.0095 | 0.0024 | 0.037 | 0.0012 | 0.0016 | 0.0002 | 0.011 | 0.0042 | 0.0014 | 0.0059 |
None | 0.0007 | 0 | 0.0079 | 0.0004 | 0.0001 | 0.0001 | 0.0009 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0.0002 | 0 |
Nuclear Periphery | 0.012 | 0.0075 | 0.0136 | 0.003 | 0.0044 | 0.0143 | 0.0053 | 0.0177 | 0.0062 | 0.0009 | 0.0136 | 0.0113 | 0.008 | 0.0179 | 0.0024 | 0.0024 | 0.0032 | 0.012 |
Nucleolus | 0.003 | 0.0006 | 0.0008 | 0.0005 | 0.0005 | 0.0003 | 0.0025 | 0.0001 | 0.0007 | 0.0003 | 0.0001 | 0.0008 | 0.0009 | 0.0003 | 0.0016 | 0.0008 | 0.0006 | 0.0007 |
Nucleus | 0.0034 | 0.0021 | 0.001 | 0.0007 | 0.0009 | 0.0005 | 0.0043 | 0.0003 | 0.0008 | 0.0003 | 0.0002 | 0.0008 | 0.0015 | 0.001 | 0.0007 | 0.001 | 0.0006 | 0.0004 |
Peroxisomes | 0.0026 | 0 | 0.0007 | 0.0012 | 0.0001 | 0.0016 | 0.0024 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0008 | 0 | 0.0002 | 0.0001 | 0.0156 | 0.0001 |
Punctate Nuclear | 0.0011 | 0 | 0.0001 | 0.0002 | 0 | 0.0005 | 0.0012 | 0 | 0.0002 | 0 | 0 | 0.0004 | 0.0009 | 0 | 0.0002 | 0 | 0.0021 | 0 |
Vacuole | 0.2704 | 0.287 | 0.2665 | 0.2547 | 0.225 | 0.2 | 0.2255 | 0.181 | 0.2026 | 0.2663 | 0.1379 | 0.1377 | 0.2417 | 0.2115 | 0.2446 | 0.244 | 0.2032 | 0.138 |
Vacuole Periphery | 0.6175 | 0.694 | 0.6749 | 0.6333 | 0.6521 | 0.7615 | 0.7146 | 0.7982 | 0.767 | 0.7249 | 0.805 | 0.8368 | 0.7211 | 0.7625 | 0.7266 | 0.7334 | 0.7529 | 0.8366 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 34.073 | 21.6969 | 48.3002 | 46.3367 | 41.1513 | 31.7216 | 35.016 | 30.1825 | 32.1046 | 38.7113 |
Translational Efficiency | 0.9159 | 1.1855 | 0.6932 | 0.5764 | 0.5322 | 0.7515 | 0.5849 | 0.5428 | 0.6884 | 0.6321 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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