ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0.13 0.16 0.17 0.19 0.23 0.22 0.25 0 0 0.05 0.07 0.1 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.06 0.05 0.05 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.09 0.1 0 0 0 0 0 0
Mitochondria 0 0 0.1 0.07 0.09 0.19 0.14 0.16 0.24 0.2 0.31 0.26 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.08 0.08 0.07 0 0 0.07 0 0 0 0 0 0 0 0 0.09 0.07 0.07 0.08 0.05 0.05 0 0.05 0.06 0.05
Nuclear Periphery 0.05 0 0.16 0.25 0.07 0.09 0.11 0.08 0.08 0.07 0.07 0.05 0.09 0.14 0.12 0.15 0.12 0.08 0 0 0.1 0.06 0.05 0
Nucleolus 0.09 0.06 0.07 0 0 0 0 0 0 0 0 0 0.06 0.06 0.05 0.06 0.07 0.11 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.83 0.88 0.75 0.81 0.81 0.7 0.67 0.62 0.6 0.55 0.51 0.51 0.85 0.87 0.83 0.85 0.81 0.77 0.86 0.87 0.76 0.76 0.72 0.75
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 3 5 4 7 3 4 0 1 0 0 0 0 1 0 0 0 0 0
Bud 0 0 0 0 0 5 3 5 0 2 5 7 0 0 1 1 0 0 3 6 3 2 3 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 0 0 4 2 5 23 15 7 10 9 13 0 0 1 0 1 1 0 0 0 0 0 0
Cytoplasm 19 14 23 14 36 79 136 143 103 155 115 157 15 20 19 17 23 14 0 1 1 0 0 0
Endoplasmic Reticulum 0 1 3 4 4 13 27 48 28 34 33 36 6 2 0 3 9 2 0 0 0 1 2 1
Endosome 1 6 13 13 17 5 22 13 3 7 3 1 6 12 5 2 1 1 8 14 20 8 18 25
Golgi 4 18 10 15 9 2 9 9 3 8 1 4 14 7 5 20 21 16 0 0 8 1 2 6
Mitochondria 9 12 54 29 55 116 127 129 128 136 163 163 0 6 11 7 3 3 1 2 10 5 4 9
Nucleus 34 49 39 8 18 41 22 19 17 20 14 19 17 19 33 16 16 13 22 27 25 17 16 21
Nuclear Periphery 22 21 84 111 41 55 94 68 46 50 36 34 36 65 42 34 28 13 9 11 58 19 13 16
Nucleolus 38 37 40 16 16 6 12 12 1 7 1 4 25 30 19 14 15 17 10 11 7 13 10 17
Peroxisomes 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1 0 0 0 2 0 1 2 1 1
Vac/Vac Membrane 360 514 399 355 477 417 586 506 328 369 268 322 329 416 300 196 185 124 402 539 456 247 202 329
Unique Cell Count 436 582 534 438 586 597 876 822 544 669 528 636 387 478 362 231 228 161 466 618 597 324 280 438
Labelled Cell Count 488 672 665 569 677 745 1064 972 669 805 651 764 448 579 437 311 302 205 466 618 597 324 280 438


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.7 20.4 12.6 13.8 11.1 9.1 9.0 8.9 8.4 7.5 7.8 8.2 20.0 16.5 14.0 22.9 23.1 23.1 19.1 18.4 19.8
Std Deviation (1e-4) 5.9 5.5 4.9 4.0 3.1 3.0 2.9 2.8 2.6 2.7 2.5 2.6 6.4 5.2 5.6 8.4 8.8 9.0 6.4 6.2 6.1
Intensity Change (Log2) 0.14 -0.18 -0.47 -0.47 -0.5 -0.59 -0.75 -0.69 -0.61 0.67 0.39 0.16 0.86 0.88 0.88 0.6 0.55 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 3.8 3.1 0 0 0 3.3 0 0 0 0 0 0
Cytoplasm -0.9 1.4 5.2 6.5 7.2 7.5 9.2 8.5 9.6 -0.3 -0.1 0.7 1.7 3.1 2.2
Endoplasmic Reticulum 0 0 2.3 3.2 5.0 4.5 4.5 5.1 4.8 0 0 0 0 0 0
Endosome 0.5 0.5 -2.1 0.1 -1.1 -2.6 -1.9 -2.5 -3.6 -0.9 0.1 -1.1 0 0 0
Golgi 1.5 -0.4 -2.5 -1.3 -1.2 -2.0 -1.0 -2.7 -1.9 1.6 -0.5 -0.6 4.4 4.7 4.7
Mitochondria -1.9 -0.4 4.4 2.4 2.9 5.9 4.8 8.4 6.8 -6.4 -6.0 -4.0 -3.3 -4.2 -3.3
Nucleus -4.0 -3.2 -0.3 -4.3 -4.4 -3.1 -3.4 -3.5 -3.4 -1.8 -2.3 1.0 -0.2 -0.1 0.3
Nuclear Periphery 3.7 -4.6 -3.3 -2.7 -4.3 -3.7 -4.5 -4.6 -5.9 -2.9 -1.0 -1.7 -0.4 -1.2 -2.5
Nucleolus -2.6 -3.7 -5.5 -5.9 -5.7 -6.3 -5.7 -6.2 -6.1 -0.6 -0.8 -1.3 -0.7 -0.4 1.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.4 2.7 -1.8 -3.1 -5.0 -5.1 -7.0 -8.1 -8.4 3.8 4.9 2.9 3.1 1.9 0.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.6875 15.0232 13.733 11.1013 9.9027 13.281 14.3418 18.1927 16.1969 15.6396 14.9718 16.5158 13.2101 17.0882 15.4223 13.9441 13.9072 14.9784
Actin 0.0064 0 0.0002 0.0005 0.0001 0 0.0089 0 0.0002 0 0 0.001 0.0029 0 0.0005 0.0001 0.0012 0.0002
Bud 0.0003 0.0001 0.0001 0.0001 0.0002 0 0.0001 0 0.0002 0 0 0.0002 0 0 0.0001 0.0001 0 0
Bud Neck 0.0002 0 0 0.0005 0 0 0.0002 0 0 0 0 0.0001 0.0001 0 0 0 0 0
Bud Periphery 0.0005 0.0002 0.0002 0.0001 0.0004 0 0.0004 0 0.0013 0.0001 0.0001 0.0005 0.0001 0 0.0003 0.0004 0.0001 0.0002
Bud Site 0.0011 0.0003 0.0001 0.0003 0.0002 0 0.0004 0 0.0001 0 0 0.0001 0.0001 0 0.0002 0.0001 0.0001 0.0001
Cell Periphery 0.0008 0.001 0.001 0.0004 0.0009 0.0004 0.0015 0.0005 0.0014 0.001 0.0003 0.0004 0.0008 0.0005 0.0012 0.0009 0.0006 0.0007
Cytoplasm 0.0022 0.0004 0.0005 0.003 0.0008 0.0002 0.001 0 0.0012 0 0 0.0002 0.0005 0.0001 0.0001 0.0002 0.0001 0
Cytoplasmic Foci 0.0135 0.0002 0.0032 0.011 0.0017 0.0072 0.0035 0 0.0005 0 0.0001 0.0002 0.0033 0.0001 0.0009 0.0003 0.0043 0.0005
Eisosomes 0.0004 0 0 0 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0014 0.0002 0.0003 0.0013 0.0007 0.0001 0.0008 0.0002 0.0014 0.0001 0.0002 0.0005 0.0006 0.0001 0.0002 0.0005 0.0001 0.0001
Endosome 0.0329 0.0028 0.0092 0.0565 0.0675 0.0108 0.0084 0.0012 0.0052 0.0033 0.0032 0.0033 0.0077 0.0019 0.0056 0.0061 0.0034 0.0021
Golgi 0.0063 0.0001 0.0055 0.0234 0.0025 0.0009 0.0017 0 0.0003 0 0.0007 0.0005 0.0011 0.0001 0.0005 0.0004 0.0009 0.0003
Lipid Particles 0.0162 0.0005 0.003 0.0046 0.0054 0.001 0.0059 0.0002 0.0008 0.0004 0.0015 0.0041 0.006 0.0036 0.003 0.005 0.0093 0.0017
Mitochondria 0.0071 0.0028 0.011 0.0044 0.0366 0.0007 0.0105 0.0004 0.0095 0.0024 0.037 0.0012 0.0016 0.0002 0.011 0.0042 0.0014 0.0059
None 0.0007 0 0.0079 0.0004 0.0001 0.0001 0.0009 0 0.0003 0 0 0.0001 0.0002 0 0 0.0001 0.0002 0
Nuclear Periphery 0.012 0.0075 0.0136 0.003 0.0044 0.0143 0.0053 0.0177 0.0062 0.0009 0.0136 0.0113 0.008 0.0179 0.0024 0.0024 0.0032 0.012
Nucleolus 0.003 0.0006 0.0008 0.0005 0.0005 0.0003 0.0025 0.0001 0.0007 0.0003 0.0001 0.0008 0.0009 0.0003 0.0016 0.0008 0.0006 0.0007
Nucleus 0.0034 0.0021 0.001 0.0007 0.0009 0.0005 0.0043 0.0003 0.0008 0.0003 0.0002 0.0008 0.0015 0.001 0.0007 0.001 0.0006 0.0004
Peroxisomes 0.0026 0 0.0007 0.0012 0.0001 0.0016 0.0024 0 0.0001 0 0.0001 0 0.0008 0 0.0002 0.0001 0.0156 0.0001
Punctate Nuclear 0.0011 0 0.0001 0.0002 0 0.0005 0.0012 0 0.0002 0 0 0.0004 0.0009 0 0.0002 0 0.0021 0
Vacuole 0.2704 0.287 0.2665 0.2547 0.225 0.2 0.2255 0.181 0.2026 0.2663 0.1379 0.1377 0.2417 0.2115 0.2446 0.244 0.2032 0.138
Vacuole Periphery 0.6175 0.694 0.6749 0.6333 0.6521 0.7615 0.7146 0.7982 0.767 0.7249 0.805 0.8368 0.7211 0.7625 0.7266 0.7334 0.7529 0.8366

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 34.073 21.6969 48.3002 46.3367 41.1513 31.7216 35.016 30.1825 32.1046 38.7113
Translational Efficiency 0.9159 1.1855 0.6932 0.5764 0.5322 0.7515 0.5849 0.5428 0.6884 0.6321

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication
Localization
Cell Percentages vacuolar membrane (91%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

YML018C

YML018C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yml018c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available