Standard name
Human Ortholog
Description Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0 0 – 0 0 0 0 0.09 0.1 0.09 0.1 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.09 0.06 0.08 0.08 – 0 0 0 0 0.05 0 0.09 0.08 0.09 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.17 0.22 0.2 0.24 0.47 0.41 0.35 – 0.53 0.53 0.54 0 0 0 0.08 0.08 0.09 0 0 0 0.06 0.1 0.17
Nucleus 0 0.06 0 0.06 0 0 0 0 – 0 0.06 0 0.12 0 0 0.14 0.12 0.08 0 0 0 0 0 0
Nuclear Periphery 0.73 0.75 0.72 0.67 0.59 0.39 0.4 0.36 – 0.45 0.39 0.44 0.7 0.78 0.67 0.41 0.41 0.47 0.89 0.83 0.88 0.83 0.74 0.67
Nucleolus 0.3 0.16 0.3 0.24 0.39 0.35 0.39 0.45 – 0.4 0.39 0.37 0.3 0.16 0.21 0.31 0.41 0.43 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.17 0.1 0.09 0.1 0.07 0.06 0.06 – 0 0 0 0.1 0.21 0.22 0.19 0.18 0.17 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 1 1 0 1 1 – 0 0 0 0 0 1 1 0 0 0 0 1 1 2 3
Bud 0 0 0 0 0 1 1 4 – 4 5 2 0 0 0 0 1 0 2 0 0 2 5 5
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 2 3 1 – 5 3 1 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 2 0 0 0 0 0 – 1 0 0 0 2 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 6 8 10 7 14 7 12 – 10 14 3 4 26 18 20 33 5 0 0 0 0 0 0
Endoplasmic Reticulum 1 0 10 5 13 3 6 10 – 11 6 7 1 0 2 6 5 3 0 0 0 0 3 4
Endosome 5 0 14 1 23 22 44 58 – 8 3 14 4 13 8 20 27 13 1 2 10 5 13 25
Golgi 0 0 0 0 1 0 1 5 – 0 1 1 0 1 0 1 0 1 0 0 1 3 6 10
Mitochondria 17 28 74 39 61 178 221 246 – 302 222 320 2 2 0 17 28 13 2 3 6 19 52 98
Nucleus 4 9 7 11 3 13 20 22 – 18 25 25 12 5 5 30 41 11 1 3 1 2 3 3
Nuclear Periphery 93 121 245 129 151 148 216 255 – 256 166 259 71 219 126 87 140 65 118 140 335 276 370 386
Nucleolus 38 26 103 47 100 131 215 316 – 230 166 219 30 44 39 65 139 60 1 4 6 7 11 9
Peroxisomes 0 0 1 0 0 1 9 8 – 2 0 0 0 0 0 0 0 0 0 0 0 0 7 4
SpindlePole 0 0 0 0 2 1 8 8 – 5 1 4 0 2 1 1 5 0 0 0 2 1 3 3
Vac/Vac Membrane 23 27 35 17 26 26 34 42 – 20 14 23 10 60 41 41 61 23 3 11 10 12 15 18
Unique Cell Count 127 161 341 192 255 378 545 708 574 421 588 101 282 187 213 339 138 134 169 379 334 500 581
Labelled Cell Count 183 217 501 260 388 540 786 988 872 626 879 134 374 241 290 480 194 134 169 379 334 500 581


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.8 12.2 11.4 11.2 10.9 9.3 8.8 9.1 – 7.8 7.6 7.9 11.8 13.0 14.0 8.9 10.3 13.3 14.6 14.7 14.7
Std Deviation (1e-4) 3.7 3.5 4.5 3.7 4.4 3.7 3.4 3.5 – 3.3 3.5 3.4 2.9 3.3 4.0 3.0 3.7 4.0 3.0 2.9 3.0
Intensity Change (Log2) – – – -0.03 -0.06 -0.3 -0.38 -0.33 – -0.55 -0.59 -0.54 0.05 0.18 0.29 -0.37 -0.15 0.22 0.35 0.36 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 1.8 0.3 1.1 -1.2 -0.7 – -0.6 0.8 0 0 3.8 3.7 3.7 4.0 0
Endoplasmic Reticulum -0.2 1.4 -2.1 -2.0 -1.7 – -1.0 -1.4 -1.9 0 0 0 -0.1 -1.3 0
Endosome -2.4 2.5 1.1 2.3 2.5 – -2.6 -3.2 -1.5 0 0.3 0.1 2.5 2.1 2.3
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria -0.4 0.6 7.1 5.8 4.3 – 9.2 8.7 9.7 -4.6 -8.0 -6.9 -4.2 -4.9 -3.2
Nucleus 2.3 0 1.1 1.4 1.0 – 1.0 2.7 1.8 0 -0.3 0 5.5 5.1 3.1
Nuclear Periphery -1.1 -3.2 -8.8 -9.3 -10.9 – -8.0 -8.9 -8.2 -0.3 1.7 -1.1 -7.2 -8.0 -5.1
Nucleolus -1.4 2.3 1.3 2.8 4.5 – 3.0 2.6 2.2 -0.1 -4.3 -2.3 0.1 2.9 2.8
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -0.5 0 -1.6 -2.2 -2.5 – -4.2 -3.9 -3.9 -0.1 3.8 3.7 3.0 2.9 1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.9332 11.8861 10.8193 10.6674 10.8618 10.7017 4.5939 10.429 9.9204 9.6922 9.6816 8.7441 6.424 6.0934 5.3728 5.5397 4.5378 5.1895
Actin 0.0216 0.0003 0.0021 0.0024 0.0001 0.0001 0.0008 0.0024 0.0012 0.004 0.0002 0.0064 0.3257 0.0003 0.0004 0.001 0 0
Bud 0.0003 0 0 0.0001 0 0 0.0001 0.0005 0.0001 0.0001 0 0.0001 0.0012 0 0 0 0 0
Bud Neck 0.0008 0.0001 0.0001 0.0007 0 0 0.0005 0.0003 0 0.0001 0 0 0.0085 0 0 0.0001 0 0
Bud Periphery 0.0005 0.0001 0.0001 0.0004 0 0 0.0001 0.004 0.0007 0.0009 0 0.0002 0.0021 0.0001 0.0001 0.0003 0 0
Bud Site 0.0055 0 0.0001 0.0001 0 0 0.0004 0.0006 0 0.0001 0 0.0002 0.0349 0 0 0 0 0
Cell Periphery 0.0002 0 0 0.0001 0 0 0.0001 0.0002 0.0001 0.0003 0 0 0.0012 0 0 0.0001 0 0
Cytoplasm 0.0027 0.0001 0.0006 0.0002 0 0 0.0034 0.0003 0.0001 0.0001 0 0.0003 0.0041 0.0001 0 0.0001 0 0
Cytoplasmic Foci 0.0106 0.0003 0.0048 0.0007 0.0001 0 0.0178 0.0007 0.0005 0.0003 0.0001 0.0036 0.0283 0.0001 0.0002 0.0001 0.0001 0
Eisosomes 0.0003 0 0 0.0001 0 0 0 0.0001 0.0001 0.0007 0 0 0.0031 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0029 0.0004 0.0019 0.0017 0.0002 0.0002 0.0022 0.0009 0.0011 0.002 0.0007 0.0008 0.0122 0.0024 0.0016 0.0018 0.0002 0.0002
Endosome 0.0078 0.0003 0.0026 0.0031 0.0004 0.0002 0.0079 0.0009 0.0004 0.0013 0.0007 0.0082 0.0205 0.0006 0.0007 0.0007 0.0001 0.0001
Golgi 0.0025 0.0004 0.0008 0.0013 0.0004 0.0002 0.0021 0.001 0.0009 0.0023 0.001 0.004 0.0163 0.0003 0.0004 0.0004 0.0003 0.0001
Lipid Particles 0.0237 0.0074 0.0099 0.0041 0.0104 0.002 0.0244 0.0282 0.0165 0.0173 0.0083 0.0089 0.0398 0.0035 0.0047 0.0034 0.0084 0.0015
Mitochondria 0.0071 0.0028 0.0031 0.0022 0.0018 0.0015 0.0003 0.0022 0.0056 0.1694 0.0005 0.0019 0.0063 0.0018 0.0039 0.0021 0.0001 0.0004
None 0.0032 0.0001 0.0005 0.0004 0.0001 0.0001 0.0007 0.0004 0.0005 0.0009 0.0001 0.0004 0.0125 0.0002 0.0002 0.0004 0 0
Nuclear Periphery 0.8448 0.8932 0.9298 0.93 0.9412 0.9695 0.7195 0.8238 0.8871 0.7286 0.956 0.9387 0.3604 0.8912 0.9344 0.9343 0.9721 0.9744
Nucleolus 0.0034 0.0054 0.0012 0.0035 0.0064 0.0007 0.0056 0.0104 0.0025 0.0024 0.0007 0.0002 0.0136 0.002 0.0008 0.0007 0.0006 0.0002
Nucleus 0.0149 0.0487 0.0067 0.0116 0.0054 0.0079 0.0115 0.0352 0.0043 0.0117 0.0013 0.0009 0.0064 0.011 0.0041 0.0049 0.0008 0.0009
Peroxisomes 0.0036 0.0001 0.0006 0.0001 0.0001 0 0.0009 0.0002 0.0002 0.0011 0 0.0028 0.0101 0 0.0001 0.0001 0 0
Punctate Nuclear 0.0176 0.0092 0.0033 0.0043 0.0018 0.0006 0.1813 0.0037 0.0089 0.004 0.0019 0.0026 0.0715 0.0019 0.0046 0.0011 0.0029 0.0003
Vacuole 0.0025 0.0003 0.0005 0.0017 0.0005 0.0002 0.0011 0.0021 0.0009 0.0014 0.0002 0.0003 0.0022 0.0013 0.0005 0.0009 0.0001 0.0002
Vacuole Periphery 0.0238 0.0306 0.0313 0.031 0.0309 0.0167 0.0194 0.0818 0.0681 0.051 0.0281 0.0194 0.0194 0.083 0.0432 0.0475 0.0144 0.0216

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 49.6196 42.6026 29.0096 36.2423 35.2564 42.0272 46.8763 45.4908 38.8324 49.8393
Translational Efficiency 0.6051 0.6134 0.7645 0.4852 0.621 0.6548 0.5872 0.4945 0.5229 0.5122

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1
Localization
Cell Percentages nuclear periphery (93%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ndc1

Ndc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ndc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available