ORF
Human Ortholog
Description Putative protein of unknown function; has some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0.11 0.13 0.07 0.06 0 0 0 0 0 0 0 0.12 0.12 0 0 0.06 0.14 0.12 0.1 0.07 0.09 0.1 0.13
Bud 0.1 0 0.09 0.09 0.05 0.06 0.11 0.08 0.07 0.07 0.09 0.06 0 0.07 0 0 0.05 0.08 0 0 0 0 0 0
Bud Neck 0 0 0.07 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.25 0.38 0.43 0.47 0.59 0.39 0.31 0.32 0.26 0.37 0.24 0.32 0.48 0.54 0.57 0.43 0.46 0.45 0.05 0.09 0.07 0.1 0.1 0.1
Endoplasmic Reticulum 0.07 0.1 0.08 0 0 0 0 0 0 0 0 0 0.1 0 0 0.07 0.08 0 0 0 0 0 0 0
Endosome 0 0.11 0 0.05 0.07 0.06 0 0.06 0 0 0 0 0.1 0.14 0.28 0.2 0.21 0.22 0.25 0.19 0.15 0.15 0.16 0.16
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0 0 0.05
Mitochondria 0.7 0.27 0.09 0.19 0.1 0.51 0.64 0.56 0.74 0.58 0.81 0.55 0 0 0 0.05 0.09 0.11 0.33 0.34 0.48 0.4 0.42 0.37
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0.06 0 0.05 0 0 0.05
SpindlePole 0 0 0.21 0.19 0.15 0.11 0.09 0.14 0.11 0.13 0.06 0.17 0.17 0.14 0.15 0.18 0.14 0.1 0 0 0.05 0.06 0.06 0.05
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.06 0.05 0 0.08 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 12 17 17 9 9 0 4 1 0 1 6 0 28 38 2 12 11 30 40 25 15 27 45 60
Bud 25 6 11 12 8 11 30 17 26 18 31 16 9 21 3 2 9 16 5 8 2 6 9 8
Bud Neck 0 0 9 2 10 1 4 2 4 2 6 3 10 8 1 0 1 2 6 7 1 7 6 6
Bud Site 0 1 0 3 0 1 7 6 11 8 4 0 0 0 0 0 0 1
Cell Periphery 1 1 4 2 1 1 1 2 2 0 3 4 0 1 0 9 7 4 0 0 0 0 1 2
Cytoplasm 60 60 56 63 92 66 82 66 94 91 84 83 110 172 229 143 86 96 15 22 15 30 46 49
Endoplasmic Reticulum 16 15 10 1 1 4 3 0 5 0 6 0 22 12 5 22 15 4 2 2 4 4 6 8
Endosome 7 17 3 6 11 11 4 13 4 1 1 9 23 45 114 66 39 47 80 47 31 43 75 76
Golgi 2 3 0 1 0 0 0 0 1 0 0 0 0 1 2 2 0 2 23 17 4 12 17 25
Mitochondria 169 42 11 25 15 87 167 117 272 144 286 141 10 7 2 16 16 24 106 83 100 118 196 176
Nucleus 1 0 2 0 3 1 1 8 6 3 1 2 0 0 0 5 1 3 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 0 0 1 1 1
Nucleolus 1 0 0 0 0 0 2 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 2 3 1 9 3 4 4 6 7 8 3 7 5 4 42 12 4 0 21 4 10 12 18 22
SpindlePole 5 3 27 25 23 18 24 29 42 33 20 43 38 44 62 58 26 22 9 4 11 17 29 24
Vac/Vac Membrane 4 8 4 2 3 5 4 4 4 2 4 7 2 8 8 53 11 10 8 18 6 5 12 9
Unique Cell Count 241 156 129 133 157 170 262 209 366 249 354 257 229 321 404 329 188 211 326 248 210 297 472 478
Labelled Cell Count 306 176 155 160 179 210 337 272 478 311 456 315 257 361 470 401 226 262 326 248 210 297 472 478


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.7 5.4 5.3 5.0 4.4 3.9 4.6 4.0 3.9 3.6 4.0 5.3 5.7 5.7 7.2 6.6 6.3 5.7 5.7 6.3
Std Deviation (1e-4) 0.5 0.8 1.2 2.0 1.4 1.7 1.4 1.4 1.5 1.1 0.9 1.1 0.9 0.9 1.3 1.2 1.2 1.2 1.5 1.1 1.7
Intensity Change (Log2) -0.03 -0.11 -0.31 -0.46 -0.25 -0.45 -0.49 -0.59 -0.45 -0.02 0.06 0.07 0.42 0.29 0.22 0.07 0.06 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.7 -2.2 0 0 0 0 0 0 0 -0.3 -0.4 0 -3.8 -2.3 0.3
Bud 0.1 -1.2 0 0 0 0 0 0 0 -1.8 -0.7 0 0 -1.3 -0.3
Bud Neck -2.2 -0.2 0 0 0 0 0 0 0 -1.1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 2.6 -0.8 -2.4 -2.2 -3.8 -1.3 -4.2 -2.1 0.8 2.0 2.6 0 0.4 0.4
Endoplasmic Reticulum -2.8 0 0 0 0 0 0 0 0 0.6 -1.8 0 -0.4 0.1 -2.6
Endosome 0 1.8 0 0 0 0 0 0 0 2.7 3.6 6.2 4.8 4.8 5.0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.4 0.3 0 0 0 0 0 0 0 -1.6 -3.1 0 -1.5 0 0.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 3.5 0 0 0
SpindlePole -0.4 -1.4 0 0 0 0 0 0 0 -1.0 -1.9 -1.5 -0.8 -1.7 -2.7
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 3.8 1.1 0.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0298 0.3322 0.2057 -0.0993 0.1048 0.3111 0.4597 0.5163 0.5177 0.9396 0.6956 0.5621 0.18 0.4815 0.1782 0.7135 0.4192 0.3238
Actin 0.0047 0.0038 0.0181 0.0017 0.0036 0.0037 0.0196 0.0079 0.0219 0.0498 0.006 0.0092 0.0453 0.0165 0.0238 0.0385 0.0235 0.0143
Bud 0.0009 0.0066 0.0135 0.0011 0.0011 0.001 0.0068 0.0057 0.0067 0.0236 0.0019 0.003 0.0019 0.0067 0.0111 0.0008 0.0018 0.0018
Bud Neck 0.0006 0.0009 0.003 0.0029 0.0012 0.0083 0.004 0.0014 0.0108 0.0135 0.0028 0.0294 0.0042 0.0014 0.0083 0.0017 0.002 0.0274
Bud Periphery 0.0015 0.0048 0.0066 0.0015 0.0015 0.0011 0.0032 0.004 0.0137 0.0109 0.0019 0.0012 0.0028 0.0021 0.0115 0.0008 0.0029 0.0043
Bud Site 0.0019 0.0218 0.0169 0.0009 0.0005 0.0018 0.0153 0.0371 0.0224 0.009 0.0321 0.0014 0.0099 0.0332 0.0191 0.0014 0.0025 0.0011
Cell Periphery 0.0004 0.0007 0.0007 0.0002 0.0001 0.0003 0.0006 0.0009 0.0011 0.0005 0.0004 0.0002 0.0007 0.0006 0.0005 0.0002 0.0002 0.0002
Cytoplasm 0.1173 0.1834 0.1178 0.1659 0.1284 0.1473 0.104 0.1822 0.146 0.1639 0.1397 0.1414 0.1519 0.2062 0.1875 0.1776 0.1722 0.1351
Cytoplasmic Foci 0.1882 0.1919 0.2135 0.2132 0.1681 0.2431 0.253 0.2019 0.2182 0.1865 0.2909 0.3139 0.265 0.2468 0.2387 0.2055 0.2563 0.2786
Eisosomes 0.0003 0.0002 0.0005 0.0002 0.0001 0.0001 0.0007 0.0002 0.0004 0.0009 0.0001 0.0001 0.0005 0.0002 0.001 0.0002 0.0003 0.0001
Endoplasmic Reticulum 0.0038 0.0049 0.0035 0.0065 0.002 0.0031 0.005 0.0027 0.0048 0.004 0.0045 0.002 0.0074 0.0038 0.0044 0.0051 0.0036 0.0051
Endosome 0.2024 0.1563 0.1767 0.2717 0.135 0.167 0.1736 0.16 0.1622 0.1112 0.2635 0.1428 0.22 0.2249 0.2036 0.3418 0.2672 0.2768
Golgi 0.0263 0.0207 0.0399 0.0329 0.018 0.0246 0.0296 0.0334 0.0315 0.0302 0.0253 0.0302 0.0452 0.0468 0.0503 0.0457 0.0559 0.0563
Lipid Particles 0.0328 0.0128 0.016 0.023 0.0279 0.0252 0.0384 0.0061 0.0109 0.0088 0.0081 0.0096 0.0327 0.0168 0.0086 0.0079 0.0112 0.0107
Mitochondria 0.0141 0.0151 0.0427 0.0459 0.0091 0.0212 0.0102 0.0101 0.0536 0.0145 0.0147 0.0178 0.01 0.0121 0.023 0.0129 0.0267 0.024
None 0.3628 0.3265 0.2506 0.1594 0.4208 0.2732 0.2383 0.3063 0.2303 0.2901 0.1216 0.2016 0.1417 0.1275 0.1454 0.1061 0.0817 0.0952
Nuclear Periphery 0.0018 0.0037 0.0013 0.0038 0.0025 0.0024 0.0087 0.0008 0.004 0.006 0.0145 0.0029 0.0076 0.0011 0.0092 0.0022 0.0173 0.0021
Nucleolus 0.001 0.0014 0.0013 0.0006 0.001 0.0012 0.0015 0.0011 0.0021 0.0038 0.0029 0.0007 0.0009 0.0024 0.0015 0.0005 0.001 0.0008
Nucleus 0.0021 0.0037 0.0021 0.0032 0.0019 0.0024 0.0035 0.0035 0.0032 0.0135 0.013 0.01 0.0041 0.0045 0.0029 0.0013 0.0029 0.0024
Peroxisomes 0.0176 0.021 0.0619 0.0401 0.0528 0.0586 0.0556 0.0229 0.0384 0.0253 0.0323 0.0631 0.0308 0.0287 0.033 0.0302 0.0567 0.0489
Punctate Nuclear 0.0052 0.0065 0.0047 0.003 0.0084 0.0058 0.0211 0.0029 0.0038 0.0227 0.008 0.0129 0.0053 0.0045 0.0046 0.0028 0.0043 0.0055
Vacuole 0.0121 0.0113 0.0068 0.0166 0.0123 0.0066 0.0056 0.0078 0.0107 0.009 0.0112 0.0052 0.0095 0.0117 0.0095 0.0139 0.0051 0.0072
Vacuole Periphery 0.0022 0.0021 0.002 0.0058 0.0034 0.002 0.0017 0.0012 0.0031 0.0024 0.0045 0.0016 0.0025 0.0016 0.0027 0.003 0.0045 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.8537 2.8926 5.7238 5.2812 2.7969 11.858 5.8494 4.277 6.5259 6.3078
Translational Efficiency 1.2354 1.7093 0.8134 0.9886 1.874 0.639 1.0608 1.0015 0.9067 1.0103

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
ORF
Human Ortholog
Description Putative protein of unknown function; has some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
Localization
Cell Percentages mitochondrion (25%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

YML037C

YML037C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yml037c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available