Standard name
Human Ortholog
Description Plasma membrane protein, component of eisosomes; long-lived protein that remains stable in eisosomes of mother cells while other eisosome proteins, Pil1p and Lsp1p, turn over; may function to anchor the eisosome in place; sporulation and plasma membrane sphingolipid content are altered in mutants; localizes to furrow-like invaginations (MCC patches)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0.52 0.63 0.52 0.43 0.39 0.31 0.3 – 0.39 0.46 0.35 0.58 0.53 0.43 0.39 0.45 0.45 0.37 0.58 0.58 0.59 0.54 0.47
Bud – 0 0.11 0.08 0.08 0.09 0.09 0.08 – 0.1 0.14 0.11 0.05 0 0 0.05 0.05 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0.51 0.33 0.34 0.2 0.15 0.09 0.12 – 0.16 0.15 0.1 0.39 0.31 0.28 0.4 0.41 0.38 0.52 0.35 0.35 0.32 0.33 0.42
Cytoplasm – 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0.06 0.07 0 0 0 0 0 0
Endoplasmic Reticulum – 0.11 0 0.07 0.09 0.09 0.05 0.09 – 0.09 0.06 0.06 0.17 0.08 0 0 0 0.05 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0.36 0.4 0.44 0.4 0.21 0.39 0.41 – 0.53 0.27 0.22 0.68 0.68 0.67 0.65 0.6 0.57 0 0 0 0 0 0
Mitochondria – 0.35 0.58 0.5 0.54 0.8 0.75 0.68 – 0.52 0.81 0.78 0.26 0.17 0.12 0.06 0.08 0.06 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0.06 0 0.09 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 185 150 95 121 121 126 144 – 176 222 141 110 149 124 119 106 105 9 266 156 95 164 154
Bud – 10 26 14 23 28 36 38 – 47 67 45 10 9 10 15 13 4 0 1 0 1 3 1
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 183 79 63 57 48 36 56 – 75 71 41 75 88 82 122 96 88 12 160 93 52 101 139
Cytoplasm – 11 2 7 4 4 3 0 – 3 1 2 3 13 9 13 15 17 0 0 0 2 1 0
Endoplasmic Reticulum – 38 8 12 24 29 19 41 – 42 28 24 33 23 10 12 7 12 0 7 1 3 3 2
Endosome – 0 0 0 0 0 0 0 – 0 0 0 0 1 0 1 1 1 0 0 1 0 2 1
Golgi – 130 94 81 113 67 162 193 – 244 129 91 129 193 195 200 142 133 0 10 2 1 8 5
Mitochondria – 124 137 92 151 251 310 323 – 236 391 316 50 48 35 17 19 14 0 11 4 2 10 9
Nucleus – 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 1 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 14 7 26 14 18 5 – 16 18 5 0 1 3 0 0 0 0 0 7 1 6 9
SpindlePole – 0 0 0 1 0 1 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0 0 – 0 0 0 0 2 0 1 0 1 0 0 0 0 0 0
Unique Cell Count 357 237 183 281 313 412 476 457 484 406 191 282 289 307 237 234 25 463 269 163 307 328
Labelled Cell Count 681 510 371 520 562 711 801 839 927 666 410 528 468 501 399 375 25 463 269 163 307 328


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 39.9 29.4 29.4 29.8 28.8 27.8 29.5 – 30.7 28.3 28.1 37.2 37.8 38.0 43.1 45.0 45.9 38.8 37.0 36.0
Std Deviation (1e-4) – 8.8 5.5 6.8 6.3 5.8 6.1 5.4 – 6.2 5.8 6.7 9.3 10.2 9.0 11.3 15.6 13.0 11.0 11.6 10.0
Intensity Change (Log2) – – – 0.0 0.02 -0.03 -0.08 0.01 – 0.06 -0.05 -0.06 0.34 0.36 0.37 0.55 0.62 0.65 0.4 0.33 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.3 -4.6 -5.7 -8.1 -8.4 – -6.2 -4.4 -7.0 -1.2 -2.4 -4.7 -5.7 -4.1 -4.0
Bud -1.1 -1.1 -0.8 -0.9 -1.3 – -0.3 1.1 0 -2.1 -3.5 -3.4 -2.7 -2.2 -4.1
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0.2 -3.4 -5.0 -7.9 -6.9 – -5.1 -5.8 -7.3 1.3 -0.5 -1.2 1.5 1.6 1.0
Cytoplasm 0 0 0 0 0 – 0 0 0 0 2.6 0 2.4 3.2 3.5
Endoplasmic Reticulum 1.5 2.4 2.7 0.8 2.6 – 2.8 1.4 1.4 4.9 2.3 0.1 0.3 -0.3 0.9
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0.9 0.1 -4.7 -0.1 0.2 – 3.4 -3.6 -4.7 5.7 6.6 6.4 5.9 4.4 3.7
Mitochondria -1.5 -0.9 5.7 4.6 2.6 – -1.5 6.5 5.4 -6.6 -9.7 -11.1 -13.4 -11.5 -12.0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes -1.0 1.4 -0.8 -0.9 -3.8 – -1.5 -1.3 -3.4 -3.4 -3.8 -3.1 -4.3 -3.8 -3.8
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 55.7627 54.314 49.1309 45.2652 50.9384 56.2031 60.4011 59.3642 53.5553 54.2519 55.267 58.9541 62.0254 61.7176 54.3416 55.2248 58.4089 61.4672
Actin 0.01 0.0117 0.0034 0.0012 0.0175 0.0043 0.0088 0.0042 0.0035 0.0003 0.0228 0.0016 0.005 0.0006 0.0009 0.0002 0.0002 0.0002
Bud 0 0 0 0 0 0 0 0.0001 0 0 0 0 0 0 0 0 0 0
Bud Neck 0.0002 0.0003 0.0004 0.0002 0 0.0001 0.0003 0.0004 0.0003 0.0002 0.0006 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0
Bud Periphery 0.0001 0 0.0001 0 0.0001 0 0.0001 0.0002 0 0 0 0 0 0 0 0 0 0
Bud Site 0.0004 0.0002 0.0016 0 0.0001 0 0.0007 0.001 0.0005 0 0.0002 0 0.0001 0.0001 0 0 0 0
Cell Periphery 0.0005 0.0007 0.0018 0.0005 0.0002 0.0002 0.0014 0.004 0.0017 0.0005 0.0003 0.0014 0.0002 0.0002 0.0003 0.0005 0.0001 0.0001
Cytoplasm 0.0002 0 0.0001 0 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0
Cytoplasmic Foci 0.0012 0 0.0005 0 0.0001 0 0.0012 0 0 0 0.0023 0 0 0 0 0 0 0
Eisosomes 0.979 0.9855 0.983 0.9901 0.9712 0.9946 0.9841 0.9889 0.9928 0.9987 0.9661 0.9937 0.9927 0.9988 0.9982 0.999 0.9996 0.9995
Endoplasmic Reticulum 0.0003 0.0001 0.0001 0.0004 0.0001 0 0.0002 0.0003 0.0002 0.0001 0 0.0001 0 0 0.0001 0 0 0
Endosome 0.0023 0 0.0008 0 0.0001 0 0.0002 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.0007 0.001 0.0006 0.0069 0.0021 0.0003 0.0005 0.0001 0.0002 0.0001 0.0003 0.0001 0.0001 0 0.0002 0.0001 0 0
Lipid Particles 0.0004 0.0001 0.0008 0.0003 0.0008 0 0.0005 0 0 0 0.0008 0 0 0 0 0 0 0
Mitochondria 0.0013 0.0002 0.0046 0.0002 0.0071 0.0003 0.001 0.0004 0.0005 0.0001 0.0039 0.0027 0.0015 0.0001 0.0001 0 0 0
None 0.0011 0 0.0001 0.0001 0.0001 0 0.0003 0.0001 0 0 0.0008 0 0.0001 0 0 0 0 0
Nuclear Periphery 0.0006 0 0.0002 0 0 0 0.0001 0.0001 0.0001 0 0 0 0 0 0 0 0 0
Nucleolus 0.0001 0 0.0001 0 0 0 0.0001 0 0 0 0.0001 0 0 0 0 0 0 0
Nucleus 0.0001 0 0.0001 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.0003 0.0001 0.0006 0 0.0003 0 0.0002 0.0001 0.0001 0 0.0017 0.0003 0.0002 0 0 0 0 0
Punctate Nuclear 0.0002 0 0.0011 0 0 0 0.0003 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.0005 0 0.0001 0 0 0 0 0.0001 0 0 0 0 0 0 0 0 0 0
Vacuole Periphery 0.0006 0 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.5605 143.2361 161.0415 99.84 45.6411 40.0354 196.3927 198.4575 156.3562 70.666
Translational Efficiency 1.2432 1.2602 1.1048 1.0915 1.2378 0.7751 1.1918 1.1056 0.9374 0.8298

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Plasma membrane protein, component of eisosomes; long-lived protein that remains stable in eisosomes of mother cells while other eisosome proteins, Pil1p and Lsp1p, turn over; may function to anchor the eisosome in place; sporulation and plasma membrane sphingolipid content are altered in mutants; localizes to furrow-like invaginations (MCC patches)
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Sur7

Sur7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sur7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available