Standard name
Human Ortholog
Description Serine esterase; homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.34 0.34 0.29 0.29 0.07 0.19 0.09 0.05 0.08 0.06 0 0.4 0.4 0.38 0.38 0.38 0.38 0.35 0.36 0.45 0.38 0.37 0.41
Bud 0 0 0.12 0.11 0.15 0.11 0.12 0.12 0.13 0.16 0.08 0.07 0.09 0.1 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.07 0.08 0.1 0.1 0.05 0 0 0 0 0.05 0.05 0.1 0.11 0.22 0.1 0.13 0.1 0 0 0.06 0.07 0.07 0.11
Cytoplasm 0.14 0.17 0.14 0.17 0.13 0.18 0.18 0.2 0.1 0.1 0.15 0.2 0.19 0.19 0.22 0.16 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0.41 0.53 0.28 0.16 0.17 0.22 0.15 0.07 0.13 0.08 0 0.42 0.33 0.23 0.05 0.05 0.07 0.43 0.34 0.21 0.24 0.25 0.2
Endosome 0.05 0 0 0 0.07 0 0.05 0 0 0 0 0 0 0 0.07 0.05 0.08 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.27 0.21 0 0 0.05 0 0 0
Mitochondria 0.1 0.16 0.3 0.55 0.38 0.57 0.6 0.62 0.76 0.82 0.81 0.11 0.16 0.21 0.08 0.15 0.08 0.08 0.09 0.11 0.12 0.12 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.07 0 0.11 0.11 0.33 0.07 0.1 0.1 0.07 0.05 0.05 0 0 0 0.11 0.09 0.18 0 0 0.05 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.09 0.14 0.12 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 77 127 79 73 20 53 39 26 40 20 3 50 111 122 62 64 73 110 159 156 69 111 139
Bud 10 14 33 29 42 31 50 68 61 53 31 9 24 31 3 4 5 0 2 0 0 2 1
Bud Neck 1 0 0 0 0 0 0 2 0 0 0 0 1 0 0 0 0 2 16 5 10 7 9
Bud Site 1 3 1 1 1 5 4 4 7 6 5 0 1 1 0 0 1
Cell Periphery 16 32 26 26 14 12 14 15 18 17 21 13 30 70 16 22 20 11 14 20 12 20 38
Cytoplasm 32 63 37 43 38 52 77 115 49 34 60 25 53 60 37 27 33 2 6 1 3 8 6
Endoplasmic Reticulum 91 201 77 41 50 62 63 40 61 25 17 53 91 73 8 8 13 134 152 73 44 76 68
Endosome 12 2 12 6 20 5 21 23 3 0 10 1 4 0 11 9 16 4 11 10 5 6 9
Golgi 5 3 2 0 0 0 0 0 0 0 0 1 0 0 24 46 40 9 16 18 5 6 9
Mitochondria 23 62 82 139 109 164 259 352 367 271 321 14 45 67 14 26 15 25 38 37 21 37 35
Nucleus 1 0 1 2 1 0 0 3 1 0 1 2 0 0 0 2 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 15 5 29 29 96 20 43 58 36 18 18 4 12 10 18 15 35 4 3 16 2 7 7
SpindlePole 2 2 8 2 11 3 10 27 10 9 7 0 4 3 15 23 22 0 0 0 0 0 0
Vac/Vac Membrane 10 7 7 0 2 1 4 10 2 5 11 0 0 1 5 5 11 3 15 8 6 13 8
Unique Cell Count 224 377 271 254 289 286 429 569 480 332 394 126 279 318 165 168 191 313 443 352 184 304 338
Labelled Cell Count 296 521 394 391 404 409 584 743 655 460 508 172 376 438 213 251 286 313 443 352 184 304 338


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 6.0 5.4 5.1 5.1 4.8 4.6 4.4 4.0 3.9 3.7 5.5 5.7 5.4 8.6 9.3 9.9 6.3 6.3 6.6
Std Deviation (1e-4) 1.4 1.0 0.8 1.3 1.6 1.4 1.5 1.4 1.1 1.1 0.8 1.0 0.9 0.9 1.7 2.1 2.3 1.2 1.3 1.4
Intensity Change (Log2) -0.06 -0.07 -0.16 -0.22 -0.29 -0.41 -0.45 -0.54 0.05 0.08 0.02 0.69 0.8 0.88 0.24 0.24 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.1 -6.9 0 0 0 0 0 0 2.1 2.6 2.4 1.8 1.9 2.0
Bud -0.3 0.8 0 0 0 0 0 0 -1.5 -1.4 -0.9 -3.8 -3.6 -3.7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.2 -2.2 0 0 0 0 0 0 0.2 0.4 4.1 0 1.1 0.3
Cytoplasm 1.0 -0.2 1.5 1.5 2.3 -1.4 -1.3 0.6 1.6 1.7 1.7 2.4 0.7 1.1
Endoplasmic Reticulum -3.4 -3.1 0 0 0 0 0 0 2.7 1.1 -1.5 -6.0 -6.1 -5.8
Endosome -1.3 1.3 0 0 0 0 0 0 0 -2.1 -3.8 1.0 0.4 1.8
Golgi 0 0 0 0 0 0 0 0 0 0 0 5.9 8.7 7.4
Mitochondria 5.7 1.9 0 0 0 0 0 0 -4.1 -3.9 -2.6 -5.3 -3.5 -5.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.3 6.4 0 0 0 0 0 0 -2.5 -2.9 -3.7 0.1 -0.6 2.3
SpindlePole 0 0.6 0 0 0 0 0 0 0 -1.2 -1.8 2.8 4.3 3.7
Vacuole 0 0 0 0 -0.8 0 -0.9 0.2 0 0 0 0 0 1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.1914 4.7543 4.9386 4.8479 3.7366 4.5358 2.4291 3.3579 3.502 2.8633 2.3312 2.8331 4.2247 4.1868 4.3522 4.2006 3.8503 3.5147
Actin 0.1755 0.1349 0.2396 0.2338 0.166 0.1831 0.171 0.1213 0.1459 0.22 0.305 0.1427 0.2411 0.1918 0.2761 0.2681 0.3451 0.2437
Bud 0.0015 0.0021 0.0043 0.0032 0.0037 0.001 0.0212 0.0019 0.0152 0.0026 0.0006 0.0012 0.0008 0.0028 0.0154 0.0008 0.0029 0.0019
Bud Neck 0.0222 0.0108 0.0333 0.0438 0.0692 0.0983 0.0304 0.0091 0.0162 0.0094 0.0076 0.1034 0.0144 0.0088 0.0189 0.0521 0.0397 0.0693
Bud Periphery 0.006 0.004 0.0188 0.0292 0.0394 0.0039 0.0138 0.0018 0.006 0.0074 0.0013 0.0039 0.0023 0.0055 0.0186 0.0088 0.0222 0.0076
Bud Site 0.0134 0.106 0.0379 0.003 0.0456 0.0029 0.0547 0.1023 0.0241 0.0138 0.0026 0.002 0.013 0.0992 0.0693 0.0029 0.0131 0.0064
Cell Periphery 0.0315 0.0159 0.0286 0.0563 0.0123 0.0179 0.0298 0.031 0.0322 0.0236 0.0195 0.0303 0.0303 0.0312 0.0443 0.063 0.046 0.0207
Cytoplasm 0.1652 0.231 0.2119 0.2407 0.151 0.2681 0.1754 0.3491 0.2875 0.2735 0.1332 0.2599 0.1853 0.2062 0.1871 0.1097 0.1292 0.3406
Cytoplasmic Foci 0.0764 0.0884 0.0766 0.0279 0.0371 0.069 0.0866 0.0645 0.0744 0.0697 0.0989 0.0972 0.0777 0.0692 0.06 0.047 0.0752 0.0652
Eisosomes 0.0054 0.0017 0.0045 0.01 0.0047 0.0033 0.0055 0.0039 0.0037 0.0035 0.006 0.0028 0.0073 0.0065 0.0093 0.0242 0.0219 0.0032
Endoplasmic Reticulum 0.0654 0.0865 0.0992 0.0562 0.1124 0.1094 0.0495 0.0487 0.0698 0.151 0.1845 0.0945 0.0715 0.0888 0.0338 0.0962 0.0492 0.0612
Endosome 0.07 0.0579 0.0442 0.0377 0.0767 0.0489 0.0345 0.0277 0.0461 0.065 0.0287 0.0431 0.0694 0.0483 0.0221 0.0159 0.0287 0.0315
Golgi 0.0567 0.071 0.0596 0.0623 0.0525 0.0616 0.0211 0.0388 0.0682 0.0499 0.0889 0.0626 0.0589 0.0605 0.0441 0.0615 0.0812 0.0366
Lipid Particles 0.1589 0.1176 0.0696 0.0354 0.093 0.0767 0.1639 0.0659 0.0796 0.0738 0.0889 0.0889 0.0888 0.0573 0.058 0.0288 0.0752 0.0308
Mitochondria 0.015 0.0062 0.0165 0.0465 0.0514 0.0161 0.0076 0.0016 0.0253 0.0045 0.0021 0.0234 0.0055 0.0047 0.0045 0.0038 0.0108 0.0053
None 0.043 0.0405 0.0308 0.0618 0.052 0.0237 0.0968 0.1086 0.0787 0.0114 0.0037 0.0247 0.097 0.1074 0.1217 0.1955 0.0374 0.0579
Nuclear Periphery 0.0342 0.004 0.0045 0.0082 0.0031 0.0022 0.0034 0.0034 0.0091 0.0018 0.0017 0.0015 0.0048 0.0012 0.0013 0.0009 0.0017 0.0015
Nucleolus 0.0009 0.0005 0.0004 0.0003 0.0002 0.0002 0.0014 0.0007 0.0006 0.0002 0.0001 0.0002 0.0006 0.0003 0.0008 0.0002 0.0005 0.0003
Nucleus 0.0063 0.0012 0.0012 0.0017 0.0007 0.0008 0.0028 0.0014 0.0047 0.0007 0.0003 0.0008 0.0008 0.0005 0.0031 0.0003 0.0006 0.001
Peroxisomes 0.031 0.0121 0.0091 0.0017 0.0205 0.007 0.0186 0.0035 0.0045 0.0096 0.0236 0.0113 0.0207 0.0062 0.006 0.0163 0.0141 0.0087
Punctate Nuclear 0.0127 0.0021 0.0034 0.0007 0.0009 0.0011 0.0062 0.0113 0.002 0.0011 0.0011 0.0015 0.0031 0.0009 0.0018 0.0006 0.0024 0.0014
Vacuole 0.0055 0.0049 0.0051 0.0219 0.0052 0.004 0.0049 0.0032 0.005 0.0067 0.0013 0.0031 0.0053 0.0024 0.0035 0.0033 0.0023 0.0047
Vacuole Periphery 0.0032 0.0007 0.001 0.0175 0.0023 0.0009 0.001 0.0003 0.0012 0.0008 0.0004 0.0008 0.0014 0.0004 0.0004 0.0003 0.0007 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.4221 27.595 29.045 30.7228 25.8305 32.8212 34.1346 32.4543 31.86 37.4555
Translational Efficiency 0.4441 0.3639 0.3688 0.3567 0.2953 0.4868 0.3752 0.3564 0.4038 0.3427

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Serine esterase; homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes
Localization
Cell Percentages ER (49%)
Cell Cycle Regulation No
Subcompartmental Group ER-1

Nte1

Nte1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nte1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available