Standard name | |
---|---|
Human Ortholog | |
Description | TFIID subunit (19 kDa); involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 6 | 2 | 0 | 1 | 1 |
Bud | 1 | 0 | 1 | 0 | 1 | 0 | – | 4 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 6 | 0 | 0 | 3 | 1 |
Bud Neck | 5 | 1 | 1 | 0 | 0 | 1 | – | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 2 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 16 | 0 | 0 | 1 | 0 | 2 | – | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 11 | 3 | 1 | 3 | 1 | 2 | 0 | 0 | 0 |
Cytoplasm | 534 | 267 | 148 | 98 | 146 | 120 | – | 119 | 87 | 84 | 55 | 82 | 61 | 133 | 155 | 313 | 195 | 229 | 444 | 224 | 97 | 66 | 50 | 112 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 17 | 14 | 14 | 0 | 1 | 3 | 0 | 0 | 0 |
Endosome | 2 | 3 | 0 | 0 | 2 | 2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 9 | 5 | 3 | 1 | 4 | 1 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 0 | 0 | 3 | 0 |
Mitochondria | 57 | 6 | 20 | 16 | 11 | 46 | – | 76 | 92 | 107 | 58 | 80 | 0 | 0 | 0 | 14 | 2 | 11 | 22 | 12 | 31 | 7 | 3 | 5 |
Nucleus | 1 | 0 | 1 | 0 | 1 | 0 | – | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 2 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 2 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 3 | 0 | 0 | 1 | 5 | 0 | – | 6 | 4 | 4 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 10 | 4 | 3 | 0 | 1 | 1 |
Vac/Vac Membrane | 3 | 3 | 0 | 0 | 1 | 0 | – | 1 | 0 | 2 | 3 | 3 | 0 | 1 | 1 | 8 | 0 | 1 | 9 | 3 | 5 | 1 | 1 | 2 |
Unique Cell Count | 562 | 274 | 161 | 103 | 159 | 144 | 166 | 147 | 154 | 104 | 135 | 62 | 135 | 157 | 325 | 198 | 239 | 538 | 278 | 161 | 81 | 75 | 129 | |
Labelled Cell Count | 627 | 281 | 172 | 117 | 167 | 173 | 210 | 185 | 203 | 128 | 173 | 62 | 136 | 159 | 368 | 220 | 264 | 538 | 278 | 161 | 81 | 75 | 129 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.5 | 4.1 | 3.1 | 3.4 | 3.9 | 2.9 | – | 2.9 | 2.7 | 2.8 | 3.2 | 2.8 | 4.4 | 4.6 | 4.6 | 5.0 | 5.4 | 5.3 | 3.9 | 5.5 | 4.6 |
Std Deviation (1e-4) | 1.3 | 1.0 | 1.1 | 2.2 | 2.2 | 1.4 | – | 1.1 | 1.5 | 1.3 | 2.2 | 1.8 | 1.1 | 1.5 | 1.8 | 1.3 | 1.7 | 2.4 | 1.3 | 2.4 | 1.0 |
Intensity Change (Log2) | – | – | – | 0.12 | 0.34 | -0.09 | – | -0.08 | -0.2 | -0.16 | 0.06 | -0.12 | 0.5 | 0.58 | 0.56 | 0.7 | 0.82 | 0.78 | 0.34 | 0.82 | 0.58 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.048 | 0.0691 | -0.1794 | -0.2871 | -0.0533 | -0.2425 | -0.7173 | -1.0723 | -0.6033 | -1.1247 | -0.9315 | -0.951 | 0.4052 | 0.5076 | 0.2307 | 0.1058 | 1.3024 | 0.6251 |
Actin | 0.0327 | 0.0021 | 0.0297 | 0.0121 | 0.0166 | 0.0099 | 0.0236 | 0.0007 | 0.0111 | 0.0011 | 0.0145 | 0.0046 | 0.0126 | 0.0019 | 0.0024 | 0.0037 | 0.0284 | 0.0027 |
Bud | 0.0018 | 0.0032 | 0.0003 | 0.0005 | 0.0007 | 0.0002 | 0.0044 | 0.0088 | 0.0132 | 0.0215 | 0.0003 | 0.0056 | 0.0008 | 0.0005 | 0.0008 | 0.0324 | 0.0059 | 0.0001 |
Bud Neck | 0.0041 | 0.0002 | 0.0009 | 0.0003 | 0.0014 | 0.0003 | 0.0024 | 0.0006 | 0.0008 | 0.0023 | 0.0002 | 0.0032 | 0.0009 | 0 | 0.0007 | 0.0008 | 0.0011 | 0.0002 |
Bud Periphery | 0.0046 | 0.0094 | 0.0004 | 0.0021 | 0.001 | 0.0005 | 0.0112 | 0.0086 | 0.0069 | 0.0124 | 0.0004 | 0.0099 | 0.0013 | 0.0006 | 0.0012 | 0.0081 | 0.0077 | 0.0001 |
Bud Site | 0.0085 | 0.0081 | 0.002 | 0.0011 | 0.0081 | 0.0002 | 0.0122 | 0.0221 | 0.0167 | 0.0181 | 0.0005 | 0.002 | 0.0036 | 0.0006 | 0.0119 | 0.0197 | 0.0041 | 0 |
Cell Periphery | 0.0003 | 0.0006 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0007 | 0.0004 | 0.0004 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0003 | 0 |
Cytoplasm | 0.0312 | 0.0288 | 0.0249 | 0.0349 | 0.0487 | 0.0683 | 0.0353 | 0.0375 | 0.0345 | 0.0337 | 0.0287 | 0.0481 | 0.0325 | 0.031 | 0.027 | 0.0381 | 0.0422 | 0.0699 |
Cytoplasmic Foci | 0.0177 | 0.0053 | 0.0095 | 0.0163 | 0.0188 | 0.0121 | 0.0368 | 0.0192 | 0.0217 | 0.0266 | 0.0173 | 0.0189 | 0.0446 | 0.0013 | 0.0022 | 0.0057 | 0.0235 | 0.0028 |
Eisosomes | 0.0004 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0005 | 0.0004 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0046 | 0.0165 | 0.0116 | 0.0009 | 0.0007 | 0.013 | 0.002 | 0.0007 | 0.001 | 0.001 | 0.0012 | 0.0007 | 0.0016 | 0.0002 | 0.001 | 0.0006 | 0.004 | 0.0026 |
Endosome | 0.0109 | 0.0008 | 0.0052 | 0.0067 | 0.0159 | 0.0047 | 0.0088 | 0.0027 | 0.0055 | 0.0007 | 0.0132 | 0.002 | 0.0246 | 0.0001 | 0.0045 | 0.0039 | 0.0458 | 0.0074 |
Golgi | 0.0033 | 0.0004 | 0.0032 | 0.0044 | 0.0148 | 0.0023 | 0.0039 | 0.0002 | 0.0021 | 0.0003 | 0.0043 | 0.0009 | 0.0085 | 0 | 0.0019 | 0.0022 | 0.0249 | 0.0016 |
Lipid Particles | 0.0101 | 0.0045 | 0.0059 | 0.0233 | 0.0267 | 0.0041 | 0.0112 | 0.001 | 0.0048 | 0.001 | 0.006 | 0.0041 | 0.0068 | 0 | 0.0059 | 0.0017 | 0.0103 | 0.0015 |
Mitochondria | 0.0056 | 0.0012 | 0.0021 | 0.0114 | 0.0165 | 0.0037 | 0.0212 | 0.003 | 0.0036 | 0.0025 | 0.0015 | 0.0077 | 0.0035 | 0.0001 | 0.0033 | 0.0131 | 0.0075 | 0.0005 |
None | 0.8249 | 0.9158 | 0.8893 | 0.8494 | 0.7686 | 0.8653 | 0.7861 | 0.8686 | 0.8271 | 0.8378 | 0.8786 | 0.8683 | 0.8348 | 0.9627 | 0.9264 | 0.8625 | 0.6984 | 0.8931 |
Nuclear Periphery | 0.0151 | 0.0002 | 0.0025 | 0.0009 | 0.0066 | 0.0046 | 0.0086 | 0.0008 | 0.0054 | 0.0012 | 0.0105 | 0.0019 | 0.0018 | 0.0001 | 0.0028 | 0.0003 | 0.0262 | 0.0078 |
Nucleolus | 0.0012 | 0.0003 | 0.0006 | 0.0034 | 0.0012 | 0.0002 | 0.0025 | 0.0022 | 0.0022 | 0.0033 | 0.0009 | 0.0016 | 0.0004 | 0 | 0.0003 | 0.0006 | 0.0017 | 0.0001 |
Nucleus | 0.0031 | 0.0003 | 0.0019 | 0.001 | 0.0241 | 0.0007 | 0.0043 | 0.0086 | 0.0055 | 0.0054 | 0.0015 | 0.0034 | 0.0012 | 0.0002 | 0.0035 | 0.0014 | 0.005 | 0.0068 |
Peroxisomes | 0.0091 | 0.0005 | 0.005 | 0.026 | 0.0246 | 0.0039 | 0.0094 | 0.0006 | 0.0198 | 0.0009 | 0.0031 | 0.0043 | 0.0114 | 0.0001 | 0.001 | 0.002 | 0.0268 | 0.0002 |
Punctate Nuclear | 0.008 | 0.0003 | 0.0022 | 0.002 | 0.0019 | 0.0046 | 0.0111 | 0.0121 | 0.0157 | 0.0278 | 0.016 | 0.0113 | 0.0062 | 0.0001 | 0.0014 | 0.0004 | 0.0318 | 0.0015 |
Vacuole | 0.0019 | 0.001 | 0.0019 | 0.0022 | 0.002 | 0.0005 | 0.0027 | 0.0008 | 0.001 | 0.0014 | 0.0003 | 0.0007 | 0.002 | 0.0002 | 0.001 | 0.0017 | 0.0018 | 0.0005 |
Vacuole Periphery | 0.0011 | 0.0002 | 0.0004 | 0.0007 | 0.0008 | 0.0005 | 0.001 | 0.0001 | 0.0006 | 0.0003 | 0.0006 | 0.0006 | 0.0007 | 0 | 0.0004 | 0.0004 | 0.0022 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.4732 | 5.8713 | 10.1657 | 13.5715 | 14.1926 | 4.3762 | 11.3782 | 10.6845 | 23.7033 | 16.2951 |
Translational Efficiency | 5.8459 | 5.6022 | 2.2636 | 2.0941 | 1.9388 | 6.8467 | 3.1487 | 2.7032 | 1.0731 | 2.0408 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|