Standard name | |
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Human Ortholog | |
Description | TFIID subunit (65 kDa); involved in RNA polymerase II transcription initiation |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 0 | 2 | 0 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 3 |
Bud Site | 5 | 1 | 1 | 2 | 1 | 3 | 3 | 8 | 5 | 7 | 3 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 7 | 2 | 3 | 2 | 3 | 3 | 8 | 7 | 1 | 4 | 1 | 29 | 56 | 69 | 34 | 18 | 24 | 0 | 0 | 0 | 0 | 2 | 4 |
Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 7 | 5 | 8 | 0 | 1 | 0 | 0 | 1 | 4 |
Endosome | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 10 | 9 | 1 | 5 | 4 | 0 | 0 | 8 | 9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 0 | 1 | 2 | 0 |
Mitochondria | 60 | 32 | 42 | 8 | 16 | 32 | 71 | 96 | 64 | 91 | 31 | 51 | 26 | 13 | 12 | 45 | 17 | 19 | 1 | 3 | 0 | 2 | 3 | 4 |
Nucleus | 269 | 398 | 82 | 18 | 31 | 41 | 62 | 118 | 51 | 94 | 29 | 43 | 346 | 393 | 351 | 455 | 320 | 207 | 221 | 377 | 82 | 188 | 366 | 258 |
Nuclear Periphery | 1 | 6 | 0 | 1 | 0 | 1 | 2 | 0 | 3 | 5 | 1 | 1 | 1 | 1 | 1 | 7 | 7 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 94 | 91 | 12 | 1 | 9 | 6 | 20 | 27 | 10 | 19 | 7 | 18 | 51 | 62 | 44 | 51 | 30 | 34 | 48 | 44 | 8 | 51 | 89 | 50 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 2 | 0 | 1 | 1 | 0 | 1 | 6 | 0 | 0 | 0 | 0 | 4 | 4 | 2 | 21 | 14 | 4 | 3 | 2 | 1 | 2 | 7 | 4 |
Vac/Vac Membrane | 1 | 5 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 0 | 9 | 12 | 5 | 8 | 17 | 1 | 8 | 19 | 23 |
Unique Cell Count | 300 | 445 | 90 | 23 | 42 | 59 | 98 | 160 | 95 | 124 | 49 | 71 | 395 | 476 | 440 | 534 | 385 | 259 | 296 | 462 | 98 | 262 | 508 | 375 |
Labelled Cell Count | 434 | 545 | 139 | 34 | 62 | 87 | 162 | 265 | 141 | 218 | 75 | 117 | 461 | 537 | 489 | 641 | 434 | 307 | 296 | 462 | 98 | 262 | 508 | 375 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.7 | 6.6 | 4.3 | 5.2 | 4.2 | 3.9 | 3.3 | 3.7 | 3.6 | 3.5 | 3.7 | 3.6 | 6.4 | 6.2 | 6.2 | 6.6 | 8.3 | 7.9 | 5.8 | 5.9 | 6.5 |
Std Deviation (1e-4) | 0.9 | 1.2 | 0.6 | 2.7 | 1.4 | 1.2 | 0.8 | 1.1 | 1.0 | 0.9 | 1.8 | 1.0 | 1.5 | 1.6 | 1.7 | 1.6 | 2.0 | 1.9 | 1.2 | 1.3 | 1.2 |
Intensity Change (Log2) | – | – | – | 0.3 | -0.03 | -0.11 | -0.35 | -0.19 | -0.23 | -0.29 | -0.19 | -0.25 | 0.58 | 0.54 | 0.55 | 0.64 | 0.96 | 0.9 | 0.44 | 0.46 | 0.61 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.8 | 2.7 | 3.4 | 1.6 | 0 | 2.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -9.9 | -12.9 | -12.6 | -9.7 | -10.9 | -8.5 |
Nucleus | -1.7 | -2.6 | -3.4 | -4.5 | -3.3 | -5.7 | -2.9 | -4.5 | -4.6 | -0.9 | -2.0 | -2.5 | -1.5 | -1.9 | -2.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.1 | -0.1 | -0.9 | -1.1 | -1.7 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0.0025 | 0.0024 | 0.0167 | 0 | 0.0071 | 0.0064 | – | – | – | – | – | – |
Bud | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0006 | 0 | 0.0013 | 0 | 0.0001 | 0.0004 | – | – | – | – | – | – |
Bud Neck | 0.001 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.002 | 0.0031 | 0.0004 | 0.0014 | 0 | 0.0005 | 0.0033 | – | – | – | – | – | – |
Bud Periphery | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0012 | 0 | 0.0012 | 0 | 0.0002 | 0.0005 | – | – | – | – | – | – |
Bud Site | 0.0003 | 0.0001 | 0.004 | 0.0001 | 0.0001 | 0.0001 | 0.0032 | 0.0008 | 0.0029 | 0 | 0.0008 | 0.0012 | – | – | – | – | – | – |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | – | – | – | – | – | – |
Cytoplasm | 0.001 | 0.0001 | 0.0111 | 0.0001 | 0.0001 | 0 | 0.0077 | 0.0099 | 0.0029 | 0 | 0.0019 | 0.0006 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0008 | 0 | 0.0042 | 0 | 0 | 0 | 0.0117 | 0.0047 | 0.0019 | 0 | 0.0128 | 0.0021 | – | – | – | – | – | – |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.001 | 0 | 0.0003 | 0 | 0 | 0 | 0.004 | 0.0008 | 0.0003 | 0 | 0.0059 | 0.0004 | – | – | – | – | – | – |
Endosome | 0.0039 | 0 | 0.0068 | 0 | 0 | 0 | 0.0123 | 0.001 | 0.0035 | 0 | 0.0327 | 0.0021 | – | – | – | – | – | – |
Golgi | 0.0002 | 0 | 0.0008 | 0 | 0 | 0 | 0.002 | 0.0004 | 0.0043 | 0 | 0.0111 | 0.0017 | – | – | – | – | – | – |
Lipid Particles | 0.0015 | 0 | 0.0004 | 0 | 0 | 0 | 0.01 | 0.0004 | 0.0011 | 0 | 0.0453 | 0.0007 | – | – | – | – | – | – |
Mitochondria | 0.0006 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0046 | 0.0013 | 0.0069 | 0.0001 | 0.0058 | 0.0025 | – | – | – | – | – | – |
None | 0.0004 | 0 | 0.0002 | 0 | 0 | 0 | 0.0083 | 0.0011 | 0.0002 | 0 | 0.0009 | 0.0003 | – | – | – | – | – | – |
Nuclear Periphery | 0.0357 | 0.0003 | 0.0006 | 0.0005 | 0.0004 | 0.0001 | 0.0291 | 0.0008 | 0.0019 | 0.0013 | 0.0031 | 0.0026 | – | – | – | – | – | – |
Nucleolus | 0.046 | 0.0247 | 0.028 | 0.0152 | 0.1183 | 0.0896 | 0.0598 | 0.0191 | 0.0192 | 0.0115 | 0.0806 | 0.1027 | – | – | – | – | – | – |
Nucleus | 0.8849 | 0.9741 | 0.9402 | 0.9831 | 0.8783 | 0.9067 | 0.8181 | 0.9551 | 0.9303 | 0.9869 | 0.7632 | 0.8611 | – | – | – | – | – | – |
Peroxisomes | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0025 | 0.0002 | 0.0019 | 0 | 0.0244 | 0.0034 | – | – | – | – | – | – |
Punctate Nuclear | 0.0194 | 0.0003 | 0.0003 | 0.0001 | 0.002 | 0.001 | 0.0129 | 0.0012 | 0.0009 | 0.0001 | 0.0017 | 0.0072 | – | – | – | – | – | – |
Vacuole | 0.0015 | 0.0001 | 0.0018 | 0.0001 | 0.0001 | 0.0002 | 0.0042 | 0.0001 | 0.0005 | 0 | 0.001 | 0.0005 | – | – | – | – | – | – |
Vacuole Periphery | 0.001 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0019 | 0.0001 | 0.0004 | 0 | 0.0007 | 0.0001 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 20.8733 | 18.016 | 15.7559 | 21.1454 | 15.8646 | 5.755 | 20.8182 | 17.7832 | 19.253 | 20.4726 |
Translational Efficiency | 0.6768 | 0.8207 | 0.9242 | 0.801 | 0.8346 | 2.1747 | 0.6388 | 0.9001 | 0.7868 | 0.7815 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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