Standard name
Human Ortholog
Description Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay;
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.05 0 0 0 0 0.06 – 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0.06 0 0 0.05 0 0.1 – 0.06 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.07 0.08 0.1 0 0.08 0.05 0.06 0.07 – 0.05 0 0.15 0.2 0.22 0.18 0.14 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0.05 0.06 0.2 0.18 0.18 0.13 0.06 0.09 0.06 0.06 0.17
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.52 0.66 0.76 0.61 0.8 0.77 0.74 0.83 – 0.85 0.88 0.09 0.06 0.28 0.11 0.06 0.13 0 0 0 0 0 0.07
Nucleus 0.69 0.6 0.46 0.39 0.45 0.29 0.37 0.31 0.37 – 0.35 0.38 0.64 0.64 0.49 0.47 0.52 0.46 0.37 0.55 0.34 0.34 0.39 0.22
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.1 0.18 0 0.08 0.07 0 0.09 0.08 0.07 – 0 0.08 0.1 0.05 0.08 0.06 0.06 0.05 0.18 0.16 0.13 0.21 0.17 0.17
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.07 0 0.1 0.11 0.13 0.07 0.12 0.09 0.1 – 0.08 0.06 0 0 0 0.09 0.11 0.12 0.15 0.06 0.13 0.14 0.1 0.13
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.1 0.08 0.1 0.05 0.06 0.13 0.1 0.14 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 1 0 1 0 1 3
Bud 0 0 6 16 15 14 32 35 32 – 26 26 0 0 8 1 1 0 1 1 2 1 2 4
Bud Neck 0 0 4 1 5 11 6 8 3 – 4 1 0 0 0 0 2 0 0 0 2 0 1 3
Bud Site 0 0 4 17 17 16 40 37 49 – 25 42 0 0 6 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 0 1 – 0 1 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 6 6 22 29 19 33 40 58 37 – 22 20 24 58 68 97 39 73 2 1 4 6 2 1
Endoplasmic Reticulum 2 0 0 0 1 1 0 0 0 – 3 0 1 0 1 17 15 14 0 2 0 1 0 3
Endosome 7 1 1 1 19 2 2 3 0 – 1 1 8 14 18 113 52 74 27 6 29 8 9 60
Golgi 2 0 0 0 0 0 1 0 0 – 0 0 0 1 0 1 1 1 3 0 0 1 0 2
Mitochondria 18 44 180 229 270 346 567 672 420 – 359 580 14 17 87 63 17 52 1 2 13 4 6 23
Nucleus 116 51 125 118 202 127 274 277 189 – 149 248 102 183 152 260 149 185 78 55 115 50 61 78
Nuclear Periphery 5 2 0 2 1 6 6 3 6 – 2 4 1 2 3 4 1 5 9 2 13 3 1 8
Nucleolus 17 15 5 24 32 16 66 75 34 – 14 54 16 15 24 33 18 22 38 16 44 30 26 59
Peroxisomes 0 0 0 0 0 2 1 2 0 – 0 0 0 3 0 10 0 10 0 0 0 0 1 0
SpindlePole 12 0 26 33 56 31 88 79 53 – 34 42 4 4 5 51 31 49 32 6 44 20 15 45
Vac/Vac Membrane 5 1 4 0 7 5 6 12 0 – 1 7 5 11 10 53 23 39 10 5 43 14 22 45
Unique Cell Count 168 85 271 303 444 432 739 903 509 423 656 160 285 310 553 285 403 213 102 337 148 158 353
Labelled Cell Count 190 120 377 470 644 610 1130 1261 825 640 1026 175 308 383 703 349 524 213 102 337 148 158 353


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 4.2 3.6 4.2 3.9 3.6 3.5 3.5 3.7 – 3.6 2.7 5.0 5.2 4.7 7.6 6.1 7.9 6.8 4.8 5.4
Std Deviation (1e-4) 0.8 1.1 3.2 7.1 3.7 5.1 4.9 4.0 6.4 – 5.4 2.1 1.1 5.8 6.0 12.8 1.4 12.3 11.8 1.4 1.5
Intensity Change (Log2) – – – 0.21 0.09 -0.01 -0.07 -0.04 0.03 – -0.02 -0.41 0.46 0.53 0.38 1.06 0.75 1.12 0.91 0.41 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.3 3.6 4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.114 2.7855 2.6093 2.2961 2.8161 2.4596 3.2545 3.0005 2.774 2.3595 2.6224 2.8437 2.4575 2.2141 2.464 2.1116 2.2863 1.8448
Actin 0.008 0.0001 0.0051 0 0.0096 0.0005 0.0109 0.0002 0.0031 0.0123 0.0158 0.0059 0.0515 0.0007 0.0002 0.0007 0.0015 0.0002
Bud 0.0002 0 0.0003 0 0.0002 0.0001 0.0008 0.0006 0.0006 0.0002 0.0005 0.002 0.0009 0.0001 0 0.0001 0.0001 0.0001
Bud Neck 0.0013 0.0003 0.0011 0.0003 0.003 0.002 0.0013 0.0006 0.0008 0.0006 0.0019 0.0029 0.0023 0.0005 0.0003 0.0005 0.0018 0.0025
Bud Periphery 0.0006 0 0.0008 0 0.0004 0.0002 0.0016 0.0007 0.0011 0.0002 0.0011 0.0053 0.0051 0.0003 0.0001 0.0001 0.0001 0.0002
Bud Site 0.0005 0.0002 0.0013 0.0001 0.0015 0.0002 0.0029 0.0082 0.0026 0.0003 0.0007 0.0019 0.0018 0.0005 0.0003 0.0001 0.0003 0.0002
Cell Periphery 0.0002 0 0.0002 0 0.0002 0.0001 0.0006 0.0003 0.0002 0 0.0002 0.0003 0.0004 0.0001 0 0 0 0
Cytoplasm 0.0017 0.0004 0.0011 0.0001 0.0016 0.001 0.0072 0.0098 0.0019 0.0006 0.0046 0.0006 0.0194 0.001 0.001 0.0008 0.0053 0.0011
Cytoplasmic Foci 0.0044 0.0001 0.0016 0 0.0007 0.0002 0.0174 0.0005 0.0028 0.0026 0.0043 0.0035 0.0096 0.0002 0.0003 0.0002 0.0006 0.0001
Eisosomes 0.0002 0 0.0001 0 0.0003 0 0.0001 0 0 0 0.0001 0.0001 0.0006 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0006 0.0002 0.0006 0 0.0034 0.0001 0.0081 0.0006 0.0018 0.0003 0.027 0.0011 0.0054 0.0002 0.0001 0.0002 0.0004 0.0001
Endosome 0.0059 0.0002 0.0029 0 0.0083 0.0008 0.039 0.0022 0.0152 0.0055 0.0306 0.008 0.0188 0.0007 0.0003 0.0005 0.0018 0.0004
Golgi 0.0009 0 0.0013 0 0.0015 0.0008 0.005 0.0001 0.0051 0.0072 0.0084 0.0035 0.0053 0.0001 0 0.0001 0.0004 0.0001
Lipid Particles 0.0039 0 0.0018 0 0.0013 0.0026 0.0101 0.0001 0.0024 0.003 0.0304 0.0051 0.0084 0.0004 0.0001 0.0002 0.0014 0.0003
Mitochondria 0.0051 0.0002 0.0048 0.0001 0.0023 0.006 0.0067 0.0004 0.0047 0.0023 0.0116 0.0115 0.0078 0.0008 0.0005 0.0003 0.0007 0.0003
None 0.0011 0.0006 0.0005 0 0.002 0.0003 0.0029 0.0007 0.0005 0.0004 0.0016 0.0003 0.0253 0.0008 0.0021 0.0008 0.0476 0.0004
Nuclear Periphery 0.0142 0.0202 0.0206 0.0048 0.0261 0.009 0.021 0.004 0.0124 0.0029 0.0715 0.0084 0.0285 0.0524 0.0316 0.08 0.0697 0.0051
Nucleolus 0.064 0.0597 0.0809 0.0771 0.1106 0.1298 0.0682 0.0582 0.0544 0.0605 0.0699 0.0864 0.0811 0.0819 0.0566 0.0514 0.0999 0.1243
Nucleus 0.8419 0.8854 0.8349 0.8724 0.7442 0.8326 0.74 0.894 0.8527 0.8436 0.6483 0.8299 0.6607 0.7985 0.8436 0.7512 0.7113 0.8316
Peroxisomes 0.0029 0 0.0019 0 0.0005 0.0005 0.0034 0 0.0006 0.0017 0.0029 0.0078 0.0037 0.0001 0 0.0001 0.0003 0.0001
Punctate Nuclear 0.0393 0.0319 0.0362 0.0448 0.0798 0.0122 0.0387 0.015 0.0325 0.0554 0.0596 0.0132 0.0563 0.0589 0.062 0.1118 0.0556 0.032
Vacuole 0.0019 0.0002 0.0013 0 0.0015 0.0004 0.0102 0.0034 0.0032 0.0001 0.004 0.0014 0.0032 0.0009 0.0003 0.0001 0.0003 0.0006
Vacuole Periphery 0.0014 0.0003 0.0008 0 0.001 0.0005 0.004 0.0004 0.0014 0.0002 0.0051 0.0008 0.0042 0.001 0.0004 0.0007 0.001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.357 11.0751 8.475 10.3305 15.812 15.8647 16.4471 14.1711 12.7602 15.9109
Translational Efficiency 0.349 0.524 0.399 0.3049 0.2595 0.3805 0.3435 0.3393 0.3113 0.2872

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔
Standard name
Human Ortholog
Description Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay;
Localization
Cell Percentages nucleus (70%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Stb4

Stb4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Stb4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available