Standard name | |
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Human Ortholog | |
Description | Mitochondrial ribosomal protein of the large subunit; located in close proximity to the polypeptide exit channel of the ribosome; mutations in human homolog MRPL44 cause childhood cardiomyopathy; human MRPL44 deficiency results in inefficient assembly of the mitochondrial ribosome, and in tissue-specific respiratory chain deficiency, manifesting as either Complex I+Complex IV or Complex IV deficiency, depending on a cell type |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | – | 0 | – | – | – | – | 8 | 18 | 0 | 0 | 4 |
Bud | – | 1 | 10 | 6 | 5 | 4 | 6 | 8 | 4 | 10 | 4 | 7 | – | – | 0 | – | – | – | – | 0 | 1 | 0 | 1 | 2 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | 1 | 0 | 0 | 0 | 1 |
Bud Site | – | 1 | 0 | 0 | 0 | 4 | 2 | 4 | 3 | 5 | 0 | 1 | – | – | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | 1 | 3 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | 1 | 2 | 5 | 3 | 10 | 8 | 12 | 7 | 3 | 8 | 3 | – | – | 0 | – | – | – | – | 0 | 1 | 0 | 0 | 0 |
Endoplasmic Reticulum | – | 1 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | – | – | 1 | – | – | – | – | 0 | 0 | 0 | 0 | 0 |
Endosome | – | 2 | 6 | 5 | 3 | 0 | 3 | 3 | 3 | 1 | 0 | 1 | – | – | 1 | – | – | – | – | 7 | 8 | 2 | 2 | 3 |
Golgi | – | 1 | 1 | 6 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | 4 | – | – | – | – | 28 | 27 | 3 | 6 | 2 |
Mitochondria | – | 129 | 233 | 156 | 151 | 219 | 240 | 249 | 216 | 212 | 183 | 149 | – | – | 14 | – | – | – | – | 104 | 182 | 47 | 29 | 33 |
Nucleus | – | 3 | 1 | 1 | 0 | 2 | 0 | 2 | 0 | 2 | 1 | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | – | 5 | 12 | 7 | 13 | 24 | 26 | 24 | 31 | 18 | 24 | 14 | – | – | 2 | – | – | – | – | 4 | 2 | 2 | 1 | 0 |
Nucleolus | – | 16 | 25 | 22 | 18 | 35 | 50 | 50 | 46 | 37 | 32 | 36 | – | – | 1 | – | – | – | – | 8 | 4 | 2 | 2 | 1 |
Peroxisomes | – | 1 | 34 | 13 | 10 | 5 | 10 | 8 | 7 | 9 | 4 | 9 | – | – | 3 | – | – | – | – | 5 | 38 | 7 | 4 | 8 |
SpindlePole | – | 1 | 1 | 4 | 5 | 2 | 3 | 5 | 4 | 11 | 3 | 3 | – | – | 0 | – | – | – | – | 1 | 3 | 2 | 1 | 0 |
Vac/Vac Membrane | – | 10 | 5 | 6 | 10 | 8 | 2 | 3 | 0 | 4 | 2 | 2 | – | – | 1 | – | – | – | – | 12 | 3 | 0 | 4 | 3 |
Unique Cell Count | 153 | 263 | 192 | 183 | 245 | 282 | 292 | 249 | 244 | 205 | 180 | 20 | 189 | 294 | 73 | 58 | 66 | |||||||
Labelled Cell Count | 173 | 333 | 235 | 222 | 313 | 353 | 369 | 322 | 313 | 263 | 225 | 27 | 189 | 294 | 73 | 58 | 66 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 12.1 | 13.1 | 12.5 | 11.0 | 9.6 | 10.1 | 10.0 | 9.7 | 9.7 | 9.5 | 9.8 | – | – | 13.3 | – | – | – | 9.7 | 13.2 | 13.0 |
Std Deviation (1e-4) | – | 3.6 | 3.0 | 3.0 | 2.6 | 2.8 | 2.9 | 2.6 | 3.0 | 2.9 | 2.8 | 2.9 | – | – | 3.4 | – | – | – | 2.5 | 3.0 | 3.9 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.24 | -0.45 | -0.38 | -0.39 | -0.42 | -0.43 | -0.47 | -0.42 | – | – | 0.02 | – | – | – | -0.43 | 0.02 | -0.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – |
Bud | -0.4 | -0.6 | -1.5 | -1.2 | -0.7 | -1.5 | 0.2 | -1.2 | 0 | – | – | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – |
Cytoplasm | 0 | 0 | 2.5 | 0 | 2.5 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – |
Mitochondria | -2.2 | -1.8 | 0.3 | -1.2 | -1.2 | -0.6 | -0.6 | 0.2 | -1.7 | – | – | -2.4 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – |
Nuclear Periphery | -0.5 | 1.1 | 2.3 | 2.1 | 1.7 | 3.2 | 1.3 | 2.9 | 1.4 | – | – | 0 | – | – | – |
Nucleolus | 0.7 | 0.1 | 1.7 | 2.8 | 2.6 | 2.9 | 1.9 | 2.0 | 3.1 | – | – | 0 | – | – | – |
Peroxisomes | -2.1 | -2.6 | -4.6 | -4.0 | -4.5 | -4.2 | -3.7 | -4.3 | -2.8 | – | – | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | – | – | 0 | – | – | – |
Vacuole | 0 | 2.1 | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0287 | 0.0087 | 0.0059 | 0.0001 | 0.1155 | 0.0022 | 0.0127 | 0.0007 | 0.0106 | 0.0011 | 0.0001 | 0.0119 | – | – | – | – | – | – |
Bud | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0.0001 | – | – | – | – | – | – |
Bud Neck | 0.0003 | 0.0002 | 0 | 0 | 0.0007 | 0 | 0.0011 | 0 | 0.0002 | 0 | 0 | 0.0001 | – | – | – | – | – | – |
Bud Periphery | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0005 | 0 | 0.0008 | 0 | 0 | 0.0003 | – | – | – | – | – | – |
Bud Site | 0.0009 | 0.0003 | 0.0002 | 0 | 0.001 | 0.0001 | 0.001 | 0 | 0.0012 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0.0001 | 0.0018 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 | – | – | – | – | – | – |
Cytoplasm | 0.0021 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0.0001 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0109 | 0.0012 | 0.0031 | 0 | 0.0044 | 0.0024 | 0.0055 | 0.0003 | 0.0037 | 0.0022 | 0.0001 | 0.0008 | – | – | – | – | – | – |
Eisosomes | 0.0005 | 0.0003 | 0.0002 | 0 | 0.0006 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.0011 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0011 | 0 | 0.0001 | 0 | 0 | 0.0023 | – | – | – | – | – | – |
Endosome | 0.0093 | 0.0054 | 0.0067 | 0.0001 | 0.0034 | 0.0038 | 0.005 | 0.0014 | 0.0102 | 0.0066 | 0.0001 | 0.0047 | – | – | – | – | – | – |
Golgi | 0.0234 | 0.021 | 0.0322 | 0.0002 | 0.0202 | 0.0164 | 0.0116 | 0.0314 | 0.011 | 0.0629 | 0.0004 | 0.02 | – | – | – | – | – | – |
Lipid Particles | 0.0472 | 0.0046 | 0.0012 | 0.0002 | 0.0224 | 0.0291 | 0.0366 | 0.0018 | 0.0146 | 0.0077 | 0.0032 | 0.0127 | – | – | – | – | – | – |
Mitochondria | 0.8225 | 0.9423 | 0.9449 | 0.9983 | 0.8231 | 0.9361 | 0.8953 | 0.9608 | 0.9328 | 0.9136 | 0.9938 | 0.9176 | – | – | – | – | – | – |
None | 0.0008 | 0 | 0 | 0 | 0.0001 | 0 | 0.0027 | 0 | 0.0001 | 0 | 0 | 0.0002 | – | – | – | – | – | – |
Nuclear Periphery | 0.006 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.0034 | 0 | 0.0002 | 0 | 0 | 0.024 | – | – | – | – | – | – |
Nucleolus | 0.0007 | 0.0017 | 0 | 0 | 0.0001 | 0 | 0.0013 | 0 | 0.0001 | 0 | 0 | 0.0001 | – | – | – | – | – | – |
Nucleus | 0.0038 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0 | 0.0007 | – | – | – | – | – | – |
Peroxisomes | 0.0352 | 0.0036 | 0.0025 | 0.0001 | 0.0054 | 0.0061 | 0.0124 | 0.0022 | 0.011 | 0.0045 | 0.0016 | 0.0013 | – | – | – | – | – | – |
Punctate Nuclear | 0.002 | 0 | 0 | 0 | 0.0006 | 0.0001 | 0.0007 | 0 | 0.0001 | 0 | 0 | 0.0002 | – | – | – | – | – | – |
Vacuole | 0.0009 | 0.0045 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0011 | 0.0001 | 0.0009 | 0.0002 | 0.0001 | 0.0003 | – | – | – | – | – | – |
Vacuole Periphery | 0.0025 | 0.0039 | 0.0021 | 0.0007 | 0.0005 | 0.0029 | 0.006 | 0.0011 | 0.0017 | 0.001 | 0.0006 | 0.0023 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.8684 | 21.0225 | 20.5915 | 25.6913 | 30.4905 | 17.8628 | 31.2461 | 32.9964 | 28.6066 | 31.9068 |
Translational Efficiency | 0.8929 | 1.1973 | 1.1325 | 0.8799 | 0.8135 | 1.9071 | 1.073 | 0.9339 | 1.2747 | 1.1989 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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