Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit; located in close proximity to the polypeptide exit channel of the ribosome; mutations in human homolog MRPL44 cause childhood cardiomyopathy; human MRPL44 deficiency results in inefficient assembly of the mitochondrial ribosome, and in tissue-specific respiratory chain deficiency, manifesting as either Complex I+Complex IV or Complex IV deficiency, depending on a cell type

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0.07
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0.2 0.15 0.09 0.05 0.12 0
Mitochondria 0.84 0.89 0.81 0.83 0.89 0.85 0.85 0.87 0.87 0.89 0.83 0.7 0.55 0.62 0.65 0.51 0.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.05 0 0.07 0.1 0.09 0.08 0.12 0.07 0.12 0.08 0.1 0 0 0 0 0
Nucleolus 0.1 0.1 0.11 0.1 0.14 0.18 0.17 0.18 0.15 0.16 0.2 0.05 0 0 0 0 0
Peroxisomes 0 0.13 0.07 0.05 0 0 0 0 0 0 0.05 0.15 0 0.13 0.1 0.08 0.13
SpindlePole 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0 0 0.05 0 0 0 0 0 0 0 0.05 0.07 0 0 0.08 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 2 0 0 8 18 0 0 4
Bud 1 10 6 5 4 6 8 4 10 4 7 0 0 1 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Bud Site 1 0 0 0 4 2 4 3 5 0 1 0
Cell Periphery 1 3 3 1 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 2 5 3 10 8 12 7 3 8 3 0 0 1 0 0 0
Endoplasmic Reticulum 1 0 1 1 0 2 0 1 1 0 0 1 0 0 0 0 0
Endosome 2 6 5 3 0 3 3 3 1 0 1 1 7 8 2 2 3
Golgi 1 1 6 2 0 1 0 0 0 0 0 4 28 27 3 6 2
Mitochondria 129 233 156 151 219 240 249 216 212 183 149 14 104 182 47 29 33
Nucleus 3 1 1 0 2 0 2 0 2 1 0 0 0 0 0 0 1
Nuclear Periphery 5 12 7 13 24 26 24 31 18 24 14 2 4 2 2 1 0
Nucleolus 16 25 22 18 35 50 50 46 37 32 36 1 8 4 2 2 1
Peroxisomes 1 34 13 10 5 10 8 7 9 4 9 3 5 38 7 4 8
SpindlePole 1 1 4 5 2 3 5 4 11 3 3 0 1 3 2 1 0
Vac/Vac Membrane 10 5 6 10 8 2 3 0 4 2 2 1 12 3 0 4 3
Unique Cell Count 153 263 192 183 245 282 292 249 244 205 180 20 189 294 73 58 66
Labelled Cell Count 173 333 235 222 313 353 369 322 313 263 225 27 189 294 73 58 66


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.1 13.1 12.5 11.0 9.6 10.1 10.0 9.7 9.7 9.5 9.8 13.3 9.7 13.2 13.0
Std Deviation (1e-4) 3.6 3.0 3.0 2.6 2.8 2.9 2.6 3.0 2.9 2.8 2.9 3.4 2.5 3.0 3.9
Intensity Change (Log2) -0.06 -0.24 -0.45 -0.38 -0.39 -0.42 -0.43 -0.47 -0.42 0.02 -0.43 0.02 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0
Bud -0.4 -0.6 -1.5 -1.2 -0.7 -1.5 0.2 -1.2 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 2.5 0 2.5 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0
Mitochondria -2.2 -1.8 0.3 -1.2 -1.2 -0.6 -0.6 0.2 -1.7 -2.4
Nucleus 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.5 1.1 2.3 2.1 1.7 3.2 1.3 2.9 1.4 0
Nucleolus 0.7 0.1 1.7 2.8 2.6 2.9 1.9 2.0 3.1 0
Peroxisomes -2.1 -2.6 -4.6 -4.0 -4.5 -4.2 -3.7 -4.3 -2.8 0
SpindlePole 0 0 0 0 0 0 3.1 0 0 0
Vacuole 0 2.1 1.0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.0287 0.0087 0.0059 0.0001 0.1155 0.0022 0.0127 0.0007 0.0106 0.0011 0.0001 0.0119
Bud 0.0003 0.0001 0.0001 0 0.0003 0 0.0002 0 0.0002 0 0 0.0001
Bud Neck 0.0003 0.0002 0 0 0.0007 0 0.0011 0 0.0002 0 0 0.0001
Bud Periphery 0.0006 0.0002 0.0002 0.0001 0.0005 0.0001 0.0005 0 0.0008 0 0 0.0003
Bud Site 0.0009 0.0003 0.0002 0 0.001 0.0001 0.001 0 0.0012 0 0 0
Cell Periphery 0.0001 0.0018 0 0 0.0001 0 0.0002 0 0.0001 0 0 0.0001
Cytoplasm 0.0021 0 0.0001 0 0.0001 0 0.0005 0 0.0001 0 0 0.0001
Cytoplasmic Foci 0.0109 0.0012 0.0031 0 0.0044 0.0024 0.0055 0.0003 0.0037 0.0022 0.0001 0.0008
Eisosomes 0.0005 0.0003 0.0002 0 0.0006 0.0002 0.0003 0.0001 0.0003 0.0001 0 0.0001
Endoplasmic Reticulum 0.0011 0 0.0001 0 0.0003 0.0001 0.0011 0 0.0001 0 0 0.0023
Endosome 0.0093 0.0054 0.0067 0.0001 0.0034 0.0038 0.005 0.0014 0.0102 0.0066 0.0001 0.0047
Golgi 0.0234 0.021 0.0322 0.0002 0.0202 0.0164 0.0116 0.0314 0.011 0.0629 0.0004 0.02
Lipid Particles 0.0472 0.0046 0.0012 0.0002 0.0224 0.0291 0.0366 0.0018 0.0146 0.0077 0.0032 0.0127
Mitochondria 0.8225 0.9423 0.9449 0.9983 0.8231 0.9361 0.8953 0.9608 0.9328 0.9136 0.9938 0.9176
None 0.0008 0 0 0 0.0001 0 0.0027 0 0.0001 0 0 0.0002
Nuclear Periphery 0.006 0 0 0 0.0003 0.0001 0.0034 0 0.0002 0 0 0.024
Nucleolus 0.0007 0.0017 0 0 0.0001 0 0.0013 0 0.0001 0 0 0.0001
Nucleus 0.0038 0.0002 0 0 0.0001 0 0.0008 0 0.0001 0 0 0.0007
Peroxisomes 0.0352 0.0036 0.0025 0.0001 0.0054 0.0061 0.0124 0.0022 0.011 0.0045 0.0016 0.0013
Punctate Nuclear 0.002 0 0 0 0.0006 0.0001 0.0007 0 0.0001 0 0 0.0002
Vacuole 0.0009 0.0045 0.0005 0.0001 0.0002 0.0002 0.0011 0.0001 0.0009 0.0002 0.0001 0.0003
Vacuole Periphery 0.0025 0.0039 0.0021 0.0007 0.0005 0.0029 0.006 0.0011 0.0017 0.001 0.0006 0.0023

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.8684 21.0225 20.5915 25.6913 30.4905 17.8628 31.2461 32.9964 28.6066 31.9068
Translational Efficiency 0.8929 1.1973 1.1325 0.8799 0.8135 1.9071 1.073 0.9339 1.2747 1.1989

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit; located in close proximity to the polypeptide exit channel of the ribosome; mutations in human homolog MRPL44 cause childhood cardiomyopathy; human MRPL44 deficiency results in inefficient assembly of the mitochondrial ribosome, and in tissue-specific respiratory chain deficiency, manifesting as either Complex I+Complex IV or Complex IV deficiency, depending on a cell type
Localization
Cell Percentages mitochondrion (88%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mrpl3

Mrpl3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrpl3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available