Standard name
Human Ortholog
Description Mitochondrial DNA-binding protein; involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation; ABF2 has a paralog, IXR1, that arose from the whole genome duplication; human homolog TFAM can complement yeast abf2 mutant, rescuing the loss-of-mitochondrial DNA phenotype in a yeast abf2 strain
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.14 0.09 0.16 0.12 0.1 0.12 0.17 0.17 0.21
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.06 – 0 0.05 0 0.05 0 0 0 0.05 0.05 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.05 0.23 0.13 0.37 0 0 – 0 0 0 0 0 0.05 0.05 0.53 0.42 0.26 0.06 0.24 0.09 0.13 0.17 0.13
Mitochondria 0.97 0.96 0.82 0.86 0.75 0.89 0.75 – 0.9 0.87 0.87 0.83 0.95 0.91 0.92 0.11 0.2 0.12 0.66 0.55 0.61 0.47 0.41 0.38
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.14 0 0 0.1 0.08 0.18 0.14 – 0.17 0.12 0.25 0.17 0.08 0.06 0.06 0.21 0.19 0.28 0 0 0 0 0 0
Peroxisomes 0 0 0.06 0.05 0 0 0 – 0 0 0 0 0.06 0.08 0.07 0.08 0.09 0.07 0.13 0.06 0.15 0.19 0.21 0.24
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0.05 0.08 0.1 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 9 4 7 4 3 3 – 1 2 1 0 6 9 6 18 9 11 38 44 29 58 105 110
Bud 0 0 2 2 3 1 2 – 2 2 1 2 1 3 3 1 1 1 1 1 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 7 3 2 1 2 2 – 1 2 0 0 4 2 5 0 2 0 0 0 0 0 0 0
Cytoplasm 0 0 3 3 6 6 19 – 9 14 1 10 0 2 2 7 5 5 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 1 1 1 0 0 – 0 1 1 1 0 0 0 3 6 5 0 0 0 0 0 0
Endosome 0 0 0 0 1 0 1 – 0 0 0 0 2 0 0 1 0 0 0 5 1 4 5 5
Golgi 2 22 43 21 59 1 14 – 4 10 2 6 7 22 22 69 42 18 20 102 21 44 101 70
Mitochondria 291 390 156 135 120 202 239 – 214 243 144 160 346 376 413 15 20 8 213 237 147 166 250 200
Nucleus 0 0 0 1 0 2 1 – 0 0 0 3 0 1 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 5 1 2 0 5 3 8 – 5 5 2 7 4 3 1 0 1 0 3 1 0 1 2 1
Nucleolus 42 18 7 15 13 41 46 – 40 33 41 33 29 25 28 27 19 19 2 3 0 4 7 4
Peroxisomes 7 11 12 8 0 1 10 – 3 12 3 1 21 32 33 11 9 5 42 23 36 68 126 129
SpindlePole 0 0 2 1 0 0 1 – 1 2 0 2 0 0 2 4 1 2 1 0 0 0 2 2
Vac/Vac Membrane 7 15 7 3 7 6 16 – 4 10 2 7 5 5 3 6 8 7 0 7 0 1 3 1
Unique Cell Count 300 406 191 157 161 227 318 238 280 165 192 364 414 451 131 101 69 325 431 240 353 608 531
Labelled Cell Count 356 473 242 199 220 268 362 284 336 198 232 425 480 518 162 123 81 325 431 240 353 608 531


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 32.7 31.3 29.1 25.8 28.8 18.5 26.5 – 25.3 24.4 24.7 23.8 31.8 33.7 34.6 41.9 39.5 41.8 30.9 34.3 37.1
Std Deviation (1e-4) 8.4 7.6 7.9 6.4 8.4 6.9 7.5 – 9.0 8.4 9.7 8.8 8.3 9.2 9.2 16.2 14.0 17.8 9.0 11.4 10.5
Intensity Change (Log2) – – – -0.18 -0.02 -0.66 -0.13 – -0.2 -0.26 -0.24 -0.29 0.13 0.21 0.25 0.53 0.44 0.52 0.08 0.24 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 4.1 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 2.4 – 1.4 2.0 0 2.0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi -2.2 2.9 -7.3 -6.3 – -6.9 -6.4 -6.0 -5.7 -8.0 -6.3 -6.8 5.6 3.4 0.6
Mitochondria 1.1 -1.6 2.1 -1.7 – 2.5 1.5 1.4 0.4 5.1 3.2 3.6 -12.4 -10.3 -10.3
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.2 1.8 4.6 3.9 – 4.3 3.1 5.8 4.3 2.0 1.2 1.3 4.9 4.3 5.7
Peroxisomes -0.5 -3.2 -3.4 -1.7 – -2.8 -1.0 -2.1 -3.1 -0.2 0.6 0.5 0.7 0.8 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0.3 -0.6 0.7 – 0 -0.1 0 0 0 0 0 0.4 1.6 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 28.5046 27.9958 27.2834 23.9284 26.8345 27.1189 35.8503 34.7652 35.7944 32.3812 33.0733 33.7165 32.5369 35.4473 33.4911 30.9984 30.7458 31.705
Actin 0.0114 0.0097 0.0087 0.0121 0.0031 0.003 0.009 0.0131 0.0079 0.0054 0.011 0.0072 0.008 0.0094 0.0036 0.0016 0.0013 0.0025
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0 0 0 0 0 0
Bud Periphery 0 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0.0001 0.0001 0 0 0 0.0001 0.0004 0 0 0 0 0.0001 0.0001 0 0 0 0
Cell Periphery 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasmic Foci 0.0008 0.0024 0.0009 0.0002 0.0008 0.0009 0.0007 0.0022 0.0008 0.0008 0.0003 0.0005 0.0016 0.003 0.0008 0.0009 0.0013 0.0003
Eisosomes 0.0015 0.001 0.003 0.0023 0.0009 0.0008 0.0012 0.0052 0.0026 0.0012 0.0014 0.0009 0.0006 0.0009 0.0009 0.0003 0.0003 0.0004
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.001 0.0034 0.001 0.0005 0.002 0.0019 0.0011 0.0027 0.0014 0.0013 0.001 0.0013 0.0006 0.0021 0.0006 0.0008 0.0003 0.0007
Golgi 0.0218 0.0546 0.0227 0.0113 0.0666 0.0273 0.025 0.0523 0.0309 0.0299 0.0313 0.0222 0.0082 0.0227 0.0096 0.0028 0.0026 0.0139
Lipid Particles 0.0341 0.0415 0.0194 0.003 0.0114 0.0174 0.027 0.0417 0.0167 0.0296 0.0183 0.0248 0.0318 0.0333 0.0321 0.0164 0.0302 0.02
Mitochondria 0.9095 0.8483 0.9238 0.9565 0.909 0.9305 0.9191 0.8453 0.9219 0.9178 0.9262 0.9273 0.9205 0.8828 0.9313 0.9443 0.9428 0.9463
None 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.0001 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.0002
Nucleolus 0.0001 0.0001 0.0001 0 0 0 0.0002 0.0028 0 0.0001 0.0001 0.0001 0.002 0.0002 0.0004 0 0.0002 0.0001
Nucleus 0 0 0 0 0 0 0 0.0001 0 0 0 0 0.0001 0 0 0 0 0
Peroxisomes 0.0119 0.0259 0.017 0.0127 0.0037 0.0127 0.0119 0.0249 0.0142 0.0098 0.0025 0.0113 0.0229 0.04 0.0156 0.0302 0.0187 0.0121
Punctate Nuclear 0 0 0 0 0 0 0 0 0 0 0 0 0.0002 0 0 0 0 0
Vacuole 0.0002 0.0008 0.0002 0.0003 0.0001 0.0002 0.0003 0.0008 0.0002 0.0002 0.0003 0.0003 0.0002 0.0005 0.0004 0.0002 0.0001 0.0002
Vacuole Periphery 0.0073 0.0118 0.0028 0.0009 0.0021 0.0052 0.0043 0.0079 0.0033 0.0036 0.0073 0.0039 0.003 0.0047 0.0045 0.0024 0.0021 0.0031

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 138.925 121.5101 128.6188 144.8356 139.9512 137.8492 169.3825 145.1124 138.1278 145.3809
Translational Efficiency 2.1889 2.8057 2.2228 1.8487 2.3134 2.3946 2.1399 2.7338 2.4141 2.5941

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial DNA-binding protein; involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation; ABF2 has a paralog, IXR1, that arose from the whole genome duplication; human homolog TFAM can complement yeast abf2 mutant, rescuing the loss-of-mitochondrial DNA phenotype in a yeast abf2 strain
Localization
Cell Percentages mitochondrion (90%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Abf2

Abf2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Abf2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available