Standard name | |
---|---|
Human Ortholog | |
Description | Putative metal transporter involved in mitochondrial iron accumulation; MMT1 has a paralog, MMT2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 9 | 9 | 6 | 1 | 1 | 0 | 12 | 20 | 12 | 7 | 23 | 22 |
Bud | 5 | 9 | 16 | 13 | 4 | 21 | 10 | 23 | 23 | 15 | 11 | 15 | 5 | 2 | 2 | 7 | 6 | 4 | 7 | 12 | 1 | 5 | 13 | 13 |
Bud Neck | 1 | 0 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 1 | 0 | 2 | 4 | 3 | 5 | 5 | 13 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 10 | 3 | 9 | 10 | 11 | 11 | 9 | 1 | 0 | 0 | 3 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 10 | 10 | 5 | 5 | 3 | 1 | 1 | 4 | 3 | 0 | 4 | 1 | 3 | 9 | 8 | 7 | 7 | 8 | 0 | 1 | 1 | 0 | 1 | 3 |
Cytoplasm | 41 | 90 | 48 | 52 | 48 | 40 | 34 | 58 | 30 | 23 | 19 | 17 | 103 | 81 | 79 | 18 | 36 | 27 | 9 | 23 | 11 | 18 | 39 | 24 |
Endoplasmic Reticulum | 7 | 8 | 3 | 4 | 1 | 2 | 4 | 3 | 2 | 2 | 3 | 1 | 13 | 7 | 2 | 4 | 7 | 4 | 1 | 3 | 2 | 2 | 4 | 4 |
Endosome | 44 | 10 | 22 | 11 | 25 | 2 | 3 | 6 | 1 | 1 | 0 | 0 | 12 | 22 | 20 | 13 | 15 | 12 | 44 | 29 | 30 | 32 | 47 | 38 |
Golgi | 9 | 2 | 4 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 13 | 23 | 37 | 4 | 9 | 17 | 20 | 12 | 10 | 4 | 10 | 17 |
Mitochondria | 323 | 251 | 199 | 264 | 214 | 429 | 492 | 615 | 423 | 488 | 440 | 538 | 56 | 91 | 60 | 11 | 20 | 11 | 338 | 243 | 245 | 235 | 309 | 332 |
Nucleus | 3 | 2 | 2 | 0 | 4 | 7 | 7 | 11 | 9 | 8 | 3 | 5 | 0 | 3 | 1 | 3 | 6 | 7 | 1 | 1 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 10 | 5 | 0 | 8 | 4 | 9 | 20 | 11 | 23 | 18 | 19 | 23 | 0 | 0 | 0 | 0 | 2 | 2 | 5 | 2 | 1 | 5 | 2 | 4 |
Nucleolus | 5 | 1 | 1 | 4 | 3 | 15 | 22 | 34 | 11 | 15 | 10 | 20 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 1 | 3 | 2 |
Peroxisomes | 26 | 10 | 23 | 8 | 13 | 4 | 3 | 14 | 3 | 5 | 2 | 2 | 2 | 13 | 7 | 13 | 15 | 7 | 24 | 8 | 26 | 21 | 32 | 21 |
SpindlePole | 14 | 4 | 32 | 14 | 15 | 19 | 11 | 36 | 13 | 15 | 15 | 13 | 9 | 8 | 5 | 13 | 10 | 20 | 10 | 7 | 7 | 11 | 16 | 21 |
Vac/Vac Membrane | 79 | 47 | 13 | 13 | 36 | 16 | 27 | 29 | 18 | 19 | 17 | 15 | 28 | 37 | 29 | 9 | 12 | 12 | 16 | 25 | 5 | 21 | 22 | 31 |
Unique Cell Count | 438 | 365 | 305 | 333 | 306 | 478 | 528 | 699 | 452 | 516 | 470 | 550 | 224 | 243 | 210 | 81 | 120 | 106 | 503 | 406 | 370 | 383 | 547 | 559 |
Labelled Cell Count | 577 | 455 | 373 | 399 | 371 | 576 | 637 | 854 | 569 | 620 | 555 | 660 | 256 | 306 | 257 | 107 | 148 | 131 | 503 | 406 | 370 | 383 | 547 | 559 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.9 | 5.1 | 4.7 | 4.3 | 4.4 | 3.6 | 3.5 | 4.0 | 3.8 | 3.5 | 3.7 | 3.8 | 5.9 | 6.1 | 6.8 | 5.3 | 5.9 | 6.8 | 5.7 | 6.1 | 6.5 |
Std Deviation (1e-4) | 1.5 | 1.2 | 1.2 | 1.1 | 1.7 | 1.3 | 1.4 | 1.2 | 1.3 | 1.1 | 1.1 | 1.1 | 1.2 | 1.3 | 1.5 | 1.5 | 1.8 | 2.1 | 1.4 | 1.7 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.08 | -0.4 | -0.41 | -0.23 | -0.31 | -0.41 | -0.33 | -0.29 | 0.33 | 0.39 | 0.54 | 0.19 | 0.34 | 0.54 | 0.29 | 0.39 | 0.46 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.8 | -2.9 | -2.6 | 0 | -0.1 | -0.6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 1.5 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | -3.2 | -4.3 | -3.5 | -4.0 | -5.6 | -5.7 | -6.7 | 7.6 | 4.8 | 5.7 | 1.4 | 3.3 | 2.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.9 | 0.8 | 0.9 | 2.5 | 1.7 | 1.3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 4.4 | 6.7 | 0 | 0 | 5.9 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -9.2 | -6.5 | -8.2 | -8.3 | -9.0 | -9.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.6 | -1.0 | -2.0 | 2.3 | 1.6 | -0.3 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.8 | -3.2 | -3.5 | 1.4 | -0.7 | 2.2 |
Vacuole | -0.2 | 3.4 | -0.7 | 0.6 | -0.1 | -0.2 | -0.4 | -0.5 | -1.2 | 3.5 | 4.4 | 3.9 | 0 | 2.3 | 2.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.1038 | 1.8394 | 1.5278 | 1.3111 | 0.8894 | 1.6393 | 3.8542 | 3.5989 | 3.8325 | 3.9027 | 3.5139 | 4.0182 | 3.7785 | 3.9938 | 4.5222 | 4.0834 | 3.9782 | 3.6908 |
Actin | 0.0131 | 0.0165 | 0.0047 | 0.0129 | 0.0037 | 0.0041 | 0.0554 | 0.0108 | 0.016 | 0.0239 | 0.0151 | 0.0162 | 0.045 | 0.0197 | 0.0252 | 0.0521 | 0.0198 | 0.0111 |
Bud | 0.0009 | 0.0106 | 0.0026 | 0.0052 | 0.0062 | 0.0022 | 0.0009 | 0.002 | 0.003 | 0.0015 | 0.0006 | 0.0015 | 0.002 | 0.0055 | 0.0034 | 0.0077 | 0.0007 | 0.0017 |
Bud Neck | 0.0007 | 0.0014 | 0.0018 | 0.0066 | 0.001 | 0.0024 | 0.0013 | 0.0014 | 0.0033 | 0.0015 | 0.0005 | 0.0019 | 0.0065 | 0.0018 | 0.0016 | 0.0109 | 0.0012 | 0.0027 |
Bud Periphery | 0.0024 | 0.0175 | 0.0059 | 0.0181 | 0.0331 | 0.0058 | 0.0026 | 0.0048 | 0.0094 | 0.0032 | 0.0022 | 0.0057 | 0.0052 | 0.0126 | 0.0092 | 0.0332 | 0.0021 | 0.0097 |
Bud Site | 0.0047 | 0.0268 | 0.0127 | 0.0023 | 0.0014 | 0.0016 | 0.0025 | 0.0325 | 0.0058 | 0.0033 | 0.0005 | 0.0019 | 0.0234 | 0.0533 | 0.0064 | 0.0143 | 0.0029 | 0.0007 |
Cell Periphery | 0.0034 | 0.0014 | 0.0008 | 0.0013 | 0.0008 | 0.0006 | 0.0011 | 0.0009 | 0.0016 | 0.0005 | 0.0002 | 0.0007 | 0.0033 | 0.0022 | 0.0017 | 0.0055 | 0.0014 | 0.0006 |
Cytoplasm | 0.014 | 0.019 | 0.0293 | 0.0697 | 0.0197 | 0.0169 | 0.0127 | 0.013 | 0.0121 | 0.0025 | 0.0013 | 0.0109 | 0.0153 | 0.0314 | 0.0207 | 0.0247 | 0.024 | 0.0132 |
Cytoplasmic Foci | 0.0528 | 0.062 | 0.0691 | 0.0311 | 0.0273 | 0.0752 | 0.0403 | 0.0541 | 0.0403 | 0.0429 | 0.025 | 0.0493 | 0.0596 | 0.0738 | 0.0473 | 0.0852 | 0.0484 | 0.0651 |
Eisosomes | 0.0009 | 0.0006 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0026 | 0.0008 | 0.001 | 0.0005 | 0.0004 | 0.0006 | 0.0011 | 0.0012 | 0.0012 | 0.0013 | 0.0021 | 0.0008 |
Endoplasmic Reticulum | 0.0036 | 0.0023 | 0.0011 | 0.0041 | 0.0016 | 0.0018 | 0.0018 | 0.0014 | 0.0008 | 0.0003 | 0.0002 | 0.0012 | 0.0073 | 0.0026 | 0.0044 | 0.0007 | 0.0033 | 0.0023 |
Endosome | 0.1301 | 0.0875 | 0.0663 | 0.0579 | 0.0757 | 0.0803 | 0.0712 | 0.0707 | 0.0347 | 0.0504 | 0.0712 | 0.0464 | 0.1017 | 0.0823 | 0.0866 | 0.074 | 0.0975 | 0.0626 |
Golgi | 0.0498 | 0.0522 | 0.0314 | 0.0338 | 0.0349 | 0.0392 | 0.0427 | 0.0477 | 0.0322 | 0.0573 | 0.0249 | 0.0307 | 0.078 | 0.066 | 0.0344 | 0.0954 | 0.0418 | 0.0681 |
Lipid Particles | 0.0375 | 0.0114 | 0.0101 | 0.0039 | 0.0137 | 0.0288 | 0.0476 | 0.0242 | 0.01 | 0.0161 | 0.0199 | 0.0195 | 0.0349 | 0.0178 | 0.0159 | 0.0055 | 0.0247 | 0.0217 |
Mitochondria | 0.5751 | 0.5764 | 0.6522 | 0.6799 | 0.6724 | 0.6344 | 0.612 | 0.5988 | 0.716 | 0.7535 | 0.6651 | 0.6959 | 0.5002 | 0.5199 | 0.5772 | 0.4913 | 0.6091 | 0.6386 |
None | 0.0489 | 0.0511 | 0.0574 | 0.0475 | 0.0538 | 0.0319 | 0.048 | 0.0381 | 0.0572 | 0.0009 | 0.0905 | 0.033 | 0.0579 | 0.0444 | 0.0784 | 0.0225 | 0.0381 | 0.0498 |
Nuclear Periphery | 0.0023 | 0.0011 | 0.0005 | 0.0008 | 0.0008 | 0.0011 | 0.0017 | 0.0008 | 0.0004 | 0.0001 | 0.0001 | 0.0004 | 0.0035 | 0.0015 | 0.0027 | 0.0002 | 0.0043 | 0.0003 |
Nucleolus | 0.0008 | 0.0034 | 0.0015 | 0.0006 | 0.0017 | 0.0013 | 0.0005 | 0.0011 | 0.0007 | 0.0001 | 0.0004 | 0.0003 | 0.0009 | 0.0008 | 0.0008 | 0.0004 | 0.0006 | 0.0003 |
Nucleus | 0.0012 | 0.003 | 0.001 | 0.0014 | 0.0039 | 0.0008 | 0.0006 | 0.0006 | 0.0006 | 0.0002 | 0.0002 | 0.0003 | 0.0015 | 0.0012 | 0.0014 | 0.0004 | 0.0011 | 0.0003 |
Peroxisomes | 0.0384 | 0.0365 | 0.0395 | 0.0084 | 0.0287 | 0.0588 | 0.0468 | 0.0833 | 0.0467 | 0.0374 | 0.0764 | 0.077 | 0.038 | 0.0505 | 0.0516 | 0.0697 | 0.0685 | 0.0438 |
Punctate Nuclear | 0.0012 | 0.005 | 0.0017 | 0.0003 | 0.0052 | 0.0025 | 0.0011 | 0.0004 | 0.0008 | 0.0002 | 0.0004 | 0.0018 | 0.0015 | 0.0005 | 0.0017 | 0.0006 | 0.0024 | 0.0008 |
Vacuole | 0.0126 | 0.01 | 0.0075 | 0.0095 | 0.0087 | 0.006 | 0.0037 | 0.0071 | 0.0051 | 0.0021 | 0.0024 | 0.0025 | 0.0071 | 0.0087 | 0.0133 | 0.0027 | 0.0035 | 0.0031 |
Vacuole Periphery | 0.0054 | 0.0045 | 0.0026 | 0.0044 | 0.0055 | 0.0039 | 0.003 | 0.0055 | 0.0023 | 0.0017 | 0.0026 | 0.0022 | 0.0061 | 0.0026 | 0.0149 | 0.0016 | 0.0025 | 0.0026 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.3157 | 8.3646 | 7.6392 | 12.254 | 16.3312 | 10.0712 | 10.4091 | 9.0014 | 11.5747 | 16.0719 |
Translational Efficiency | 1.0553 | 1.1716 | 0.9751 | 0.6068 | 0.7534 | 1.2676 | 0.7685 | 0.7565 | 0.6227 | 0.6478 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|