Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0.08 0 0 0 0 0 0 0 0 0.12 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0.06 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.07 0.05 0.05 0.05 0.05 0.06 0.05 0 0.07 0.11 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.08 0.11 0.1 0.07 0.05 0.05 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.09 0 0 0 0 0.05 0.05 0.16 0.07 0.13 0.1 0 0 0 0 0.1 0.09 0 0 0 0 0 0
Nucleus 0.08 0.17 0.15 0.09 0.12 0.1 0.1 0.09 0.11 0.12 0.14 0.1 0 0.08 0.11 0.15 0.22 0.25 0.07 0.19 0.1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.94 0.88 0.82 0.9 0.81 0.77 0.79 0.78 0.8 0.78 0.78 0.74 0.97 0.85 0.7 0.89 0.8 0.78 0.75 0.66 0.67 0.68 0.66 0.56
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.15 0.13 0.34 0.24 0.33 0.36 0.35 0.44 0.28 0.33 0.25 0.33 0.12 0.39 0.36 0.2 0.09 0.12 0.11 0 0.16 0.16 0.19 0.28
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 2 0 2 0 1 0 4 1 2 0 3 0 0 0 0 1 0 0 1 2 1 1 0
Bud Neck 1 2 6 16 5 4 6 5 5 7 0 3 4 3 9 3 0 2 0 2 1 0 0 0
Bud Site 5 1 1 1 0 1 4 4 7 8 2 10 0 1 0 0 1 2
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 3 7 7 9 18 17 15 15 13 12 12 0 8 25 4 5 4 0 0 0 0 2 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 1
Endosome 0 1 1 3 22 29 32 24 14 13 9 3 0 3 18 2 1 2 2 2 3 1 1 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 1 0 0 0 0 0
Mitochondria 1 10 1 1 1 12 17 17 45 20 27 22 0 3 2 5 12 15 0 1 2 0 0 1
Nucleus 7 20 29 17 32 28 33 31 32 34 31 22 1 9 23 19 27 41 6 23 21 1 3 6
Nuclear Periphery 0 0 0 0 2 1 1 5 1 3 3 4 0 0 0 0 0 3 0 1 0 0 0 1
Nucleolus 79 101 158 170 212 205 253 259 226 224 168 161 31 98 153 109 97 127 69 84 141 31 73 83
Peroxisomes 1 0 0 0 1 0 1 1 2 1 1 1 0 0 2 2 0 3 0 1 0 0 0 0
SpindlePole 13 15 65 46 87 95 113 147 79 95 54 73 4 45 79 25 11 19 10 4 34 7 21 41
Vac/Vac Membrane 0 1 1 1 2 2 2 2 2 2 2 2 0 1 2 0 3 5 0 2 2 0 0 0
Unique Cell Count 84 115 193 189 262 267 319 331 283 286 215 219 32 115 218 123 122 162 93 127 212 47 111 150
Labelled Cell Count 109 157 269 264 373 396 479 514 429 422 309 317 40 171 315 170 158 225 93 127 212 47 111 150


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 7.4 11.2 10.4 10.0 8.2 7.7 7.5 6.7 7.0 6.9 6.4 8.0 10.5 9.1 9.9 12.3 12.7 12.3 11.8
Std Deviation (1e-4) 2.0 2.2 4.4 3.3 2.5 3.0 2.6 2.0 2.3 2.2 2.6 1.8 2.0 5.5 4.5 3.1 4.6 5.2 2.9 4.1
Intensity Change (Log2) -0.12 -0.17 -0.46 -0.55 -0.58 -0.74 -0.69 -0.71 -0.81 -0.49 -0.09 -0.31 -0.18 0.12 0.17 0.13 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 2.2 0 0 0 0 0 -0.4 0 0 0 0 0.6 0 0 0
Bud Site 0 0 0 0 0 0 0 0 2.5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -0.1 1.5 0.9 0.5 0.9 0.5 0.9 0.9 0 1.3 3.0 0 0 -0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 3.8 4.4 4.2 3.5 2.7 2.6 0 0 0 0 3.7 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.5 2.9 2.8 5.6 3.4 4.8 4.2 0 0 0 0 4.1 4.0
Nucleus -1.8 -0.9 -1.5 -1.6 -2.0 -1.2 -1.0 -0.2 -1.5 0 -1.9 -1.4 0.1 1.6 2.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.3 -0.3 -1.3 -0.7 -1.0 -0.5 -0.9 -0.9 -2.0 2.1 0.8 -2.8 1.6 -0.5 -0.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -2.0 -0.1 0.4 0.4 2.4 -1.3 -0.1 -1.9 -0.1 -2.4 1.0 0.5 -2.6 -5.0 -4.8
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.8527 5.5212 5.2736 4.373 4.7851 5.2306 7.0257 9.9531 8.1889 7.9131 7.028 8.2808 4.1165 5.7453 5.0365 5.0206 4.5712 4.7985
Actin 0.0078 0 0.0112 0.0024 0 0 0.0247 0 0.0005 0.0006 0.0086 0 0.0018 0 0.0003 0 0.0001 0
Bud 0.0001 0 0 0.0001 0 0 0.0002 0 0 0.0001 0.0001 0 0.0001 0 0 0 0.0001 0
Bud Neck 0.001 0.0005 0.0003 0.001 0.0003 0.0003 0.0026 0.0005 0.0003 0.0006 0.0021 0.0005 0.0004 0.0033 0.0002 0.0004 0.0003 0.0013
Bud Periphery 0.0001 0 0 0.0001 0 0 0.0003 0 0 0.0001 0.0004 0 0.0001 0 0.0001 0 0.0001 0
Bud Site 0.0006 0.0001 0.0002 0.0006 0.0002 0.0001 0.0023 0.0002 0.0001 0.0015 0.0005 0.0001 0.0004 0.0002 0.0002 0.0001 0.0004 0.0001
Cell Periphery 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0009 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001
Cytoplasm 0.002 0 0.0001 0.0009 0.0002 0 0.0059 0 0.0004 0.0009 0.0001 0 0.0021 0 0 0 0.0002 0
Cytoplasmic Foci 0.0074 0.001 0.0018 0.0043 0.0036 0.0019 0.0293 0.0004 0.0009 0.0219 0.0083 0.0019 0.0101 0.0038 0.0009 0.0012 0.0212 0.0014
Eisosomes 0.0001 0 0.0001 0 0 0 0.0003 0 0.0002 0 0.0002 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0018 0 0.0003 0.0008 0 0 0.0036 0 0.0001 0.0004 0.0002 0 0.0034 0 0 0 0.0001 0
Endosome 0.0039 0 0.0032 0.0101 0.0002 0.0001 0.0218 0 0.0001 0.0008 0.0024 0.0001 0.0027 0.0002 0.0003 0.0001 0.0007 0
Golgi 0.0014 0 0.0018 0.0019 0.0001 0.0001 0.0062 0 0.0001 0.0004 0.0027 0.0001 0.0021 0.0002 0.0004 0 0.0008 0.0001
Lipid Particles 0.0156 0.0023 0.0037 0.006 0.0028 0.0075 0.0294 0.0012 0.0023 0.0062 0.0341 0.01 0.0123 0.0131 0.0014 0.0035 0.0694 0.0046
Mitochondria 0.001 0 0.0003 0.0006 0.0013 0.0004 0.004 0.0002 0.0001 0.0005 0.0061 0.0047 0.0012 0.0001 0.0093 0.0001 0.0043 0.0001
None 0.0013 0 0.0003 0.0011 0.0004 0 0.0113 0 0.0064 0.0032 0.0007 0 0.0037 0 0 0 0.0004 0
Nuclear Periphery 0.005 0 0.0005 0.0132 0.001 0.0002 0.0275 0.0001 0.0001 0.0006 0.0019 0.0002 0.0086 0 0 0 0.0007 0
Nucleolus 0.8682 0.9107 0.9045 0.8758 0.9053 0.8923 0.7169 0.8978 0.9262 0.8926 0.8317 0.9147 0.8512 0.8842 0.9198 0.946 0.8017 0.9243
Nucleus 0.0607 0.0696 0.0676 0.0466 0.0536 0.0507 0.0518 0.0935 0.0567 0.0559 0.0399 0.0556 0.0735 0.0853 0.0647 0.0469 0.0592 0.0531
Peroxisomes 0.0005 0 0.0007 0.0005 0.0002 0.0002 0.0218 0 0.0002 0.0014 0.0341 0.0005 0.0069 0.0003 0.0006 0.0001 0.0092 0.0001
Punctate Nuclear 0.0201 0.0153 0.003 0.0325 0.0298 0.0458 0.0365 0.0053 0.0051 0.0116 0.0199 0.011 0.018 0.0084 0.0014 0.0011 0.03 0.0145
Vacuole 0.0007 0.0001 0.0001 0.0007 0.0003 0.0002 0.0015 0.0003 0.0002 0.0004 0.0036 0.0002 0.0006 0.0004 0.0002 0.0003 0.0005 0.0001
Vacuole Periphery 0.0004 0 0.0001 0.0005 0.0003 0.0001 0.0018 0.0002 0 0.0001 0.0017 0.0002 0.0005 0 0.0001 0.0001 0.0004 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.7807 8.8863 13.188 18.5632 11.9678 24.128 19.8951 19.4921 20.5755 21.5926
Translational Efficiency 2.6711 3.5296 2.3317 2.5038 2.6644 1.5499 1.9718 2.2547 2.5684 1.9188

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene
Localization
Cell Percentages nucleolus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Mrpl24

Mrpl24


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrpl24-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available