Standard name
Human Ortholog
Description Protein that recognizes and binds damaged DNA during NER; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein; NER stands for nucleotide excision repair

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.1 0.21 0.2 0.2 0.16 0.09 0.11 0.09 0.08 0.08 0.11 0.29 0.28 0.36 0.14 0.13 0.14 0.05 0.07 0.05 0.19 0.21 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.08 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.53 0.29 0.14 0.46 0.35 0.63 0.71 0.65 0.66 0.76 0.8 0.72 0 0 0 0.07 0.08 0.06 0 0 0 0 0 0
Nucleus 0.58 0.76 0.64 0.42 0.45 0.46 0.51 0.55 0.54 0.51 0.5 0.49 0.66 0.61 0.56 0.59 0.55 0.55 0.65 0.69 0.54 0.57 0.37 0.32
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0.15 0.17 0.11 0.17 0.19 0.1 0.05 0.05 0.06 0.05 0 0.05 0.08 0.15 0.14 0.23 0.34 0.33 0.22 0.18 0.28 0.14 0.3 0.31
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0
Bud 0 0 1 0 3 1 13 7 10 14 9 9 0 0 0 1 0 0 0 0 1 2 5 5
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 1 4
Bud Site 0 0 0 0 0 2 9 9 4 24 9 8 0 0 0 0 1 0
Cell Periphery 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 4 37 39 41 49 45 59 36 43 24 35 91 90 177 34 19 12 5 4 10 16 77 65
Endoplasmic Reticulum 1 0 3 1 0 0 0 0 0 0 0 0 3 1 3 12 5 4 0 0 0 0 0 1
Endosome 0 0 1 3 7 3 0 1 2 1 0 0 3 4 2 12 4 7 0 1 3 0 2 5
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 1 0 1 0
Mitochondria 39 12 24 91 71 196 367 342 255 408 233 230 2 3 2 17 11 5 1 1 8 0 11 13
Nucleus 43 32 113 82 92 141 262 288 210 273 147 158 209 197 277 144 79 48 60 51 115 48 135 107
Nuclear Periphery 0 0 0 1 0 3 0 6 0 2 3 0 0 0 0 3 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 1 0 1 3 4 3 3 3 4 0 0 0 0 1 0 0 0 0 0 0 3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 0 3 2 2 0 1 1 1 0 0 0 0 1 0 5 2 15 17
Vac/Vac Membrane 11 7 19 33 38 31 27 25 24 28 10 17 25 49 67 57 49 29 20 13 59 11 111 103
Unique Cell Count 74 42 177 197 204 309 514 525 386 540 293 320 315 324 491 243 143 87 94 74 213 85 370 335
Labelled Cell Count 97 55 200 252 253 427 726 744 546 799 438 463 336 345 528 283 170 105 94 74 213 85 370 335


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.3 4.3 4.0 4.5 3.7 3.4 3.4 3.5 3.2 3.2 3.2 5.5 5.4 5.3 6.8 6.6 6.4 5.4 5.6 6.0
Std Deviation (1e-4) 0.5 0.7 1.0 1.5 2.5 1.8 1.2 1.1 1.9 1.3 1.7 1.8 0.9 1.0 0.9 1.8 1.4 1.1 1.3 1.3 1.7
Intensity Change (Log2) -0.1 0.06 -0.24 -0.36 -0.35 -0.29 -0.42 -0.46 -0.44 0.33 0.33 0.3 0.64 0.6 0.57 0.33 0.37 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 3.3 5.1 4.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2236 0.5983 0.777 1.133 0.9095 0.4754 -0.6306 -0.6999 -0.5338 -0.8111 -0.8758 -0.8271 -1.7311 -1.0841 -1.2377 -1.2384 -1.5131 -1.3747
Actin 0.0521 0.0003 0.0315 0.0005 0.0106 0.0041 0.0115 0.0001 0.0034 0.0199 0 0.0016 0.0169 0.0002 0.0154 0.0487 0.0153 0.0014
Bud 0.0005 0.0001 0.0008 0.0005 0.0008 0.0001 0.001 0.0001 0.0016 0.0009 0 0.0007 0.0001 0.0001 0.001 0.0002 0.0002 0.0003
Bud Neck 0.0042 0.0005 0.0012 0.0059 0.0016 0.0021 0.0153 0.0001 0.0005 0.0016 0.0003 0.0026 0.0006 0.006 0.0015 0.0014 0.0013 0.0038
Bud Periphery 0.0007 0.0001 0.0015 0.0004 0.001 0.0001 0.0014 0.0001 0.0017 0.0008 0 0.0004 0.0001 0 0.0017 0.0002 0.0003 0.0003
Bud Site 0.0073 0.001 0.0113 0.0045 0.0035 0.0002 0.0157 0.0005 0.0084 0.003 0.0001 0.0005 0.001 0.005 0.0051 0.0041 0.0007 0.0008
Cell Periphery 0.0002 0.0001 0.0005 0.0001 0.0001 0 0.0004 0.0001 0.0002 0.0001 0 0.0001 0.0001 0 0.0002 0.0001 0.0001 0
Cytoplasm 0.1065 0.1752 0.1016 0.1709 0.0981 0.109 0.1348 0.2124 0.1531 0.1087 0.2477 0.3714 0.1408 0.1376 0.1345 0.0986 0.1185 0.0906
Cytoplasmic Foci 0.0209 0.0029 0.0052 0.0315 0.0171 0.0069 0.0208 0.0012 0.008 0.0176 0.0041 0.0059 0.0188 0.0017 0.0064 0.0085 0.0051 0.0162
Eisosomes 0.0004 0.0001 0.0004 0 0.0001 0.0001 0.0001 0 0.0001 0.0001 0 0 0.0001 0 0.0001 0.0002 0.0002 0
Endoplasmic Reticulum 0.0066 0.0041 0.0052 0.0016 0.0106 0.0021 0.0077 0.0048 0.0051 0.0057 0.0027 0.0074 0.0065 0.0046 0.0039 0.0031 0.0045 0.0023
Endosome 0.0211 0.0028 0.0078 0.0419 0.04 0.0037 0.0203 0.0018 0.0214 0.0146 0.0089 0.013 0.0068 0.004 0.0104 0.0056 0.0143 0.003
Golgi 0.0063 0.0001 0.0028 0.0054 0.006 0.0013 0.0077 0 0.0134 0.0039 0.0001 0.0012 0.0003 0.0001 0.0042 0.0034 0.0112 0.0009
Lipid Particles 0.0071 0.0003 0.0031 0.0152 0.0137 0.005 0.0283 0.0002 0.003 0.0096 0.0001 0.0033 0.0007 0.0003 0.0047 0.0032 0.0054 0.0005
Mitochondria 0.0038 0.0005 0.0058 0.0014 0.0038 0.0021 0.0125 0.0004 0.0373 0.0056 0.0002 0.0012 0.0005 0.0004 0.0056 0.0007 0.0123 0.0004
None 0.1843 0.2182 0.1749 0.1571 0.0835 0.2047 0.1523 0.1401 0.1244 0.1149 0.18 0.0666 0.1053 0.0718 0.1386 0.1074 0.126 0.0209
Nuclear Periphery 0.0436 0.0135 0.0243 0.007 0.0671 0.0118 0.04 0.0272 0.0314 0.0375 0.0279 0.0382 0.0296 0.0318 0.0201 0.0219 0.026 0.0154
Nucleolus 0.0041 0.0041 0.0075 0.0147 0.0078 0.0063 0.0045 0.0015 0.0029 0.0017 0.0009 0.0016 0.0018 0.0022 0.0023 0.0014 0.0041 0.0027
Nucleus 0.468 0.5507 0.5869 0.4559 0.5688 0.5838 0.4846 0.6012 0.5563 0.6051 0.518 0.4511 0.6517 0.7184 0.6237 0.6661 0.6335 0.8036
Peroxisomes 0.0068 0.0001 0.0016 0.0043 0.0059 0.0045 0.0081 0 0.0068 0.0329 0 0.0007 0.0002 0.0001 0.0027 0.0039 0.0014 0.0019
Punctate Nuclear 0.0516 0.0228 0.0224 0.0758 0.0506 0.0508 0.021 0.0052 0.0122 0.0088 0.0026 0.0249 0.0146 0.0117 0.014 0.0196 0.0153 0.0335
Vacuole 0.0026 0.0023 0.0025 0.0047 0.0066 0.001 0.0073 0.0021 0.0071 0.0041 0.0056 0.0059 0.0029 0.0033 0.0028 0.0012 0.0029 0.0012
Vacuole Periphery 0.0014 0.0003 0.0009 0.0006 0.0027 0.0003 0.0046 0.0005 0.0018 0.0027 0.0008 0.0017 0.0006 0.0005 0.0011 0.0004 0.0014 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.4266 8.8385 9.182 6.7065 7.6915 1.9763 8.4898 8.7458 9.1305 7.5168
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein that recognizes and binds damaged DNA during NER; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein; NER stands for nucleotide excision repair
Localization
Cell Percentages cytoplasm (19%), nucleus (15%), mixed (63%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rad14

Rad14


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available