Standard name | |
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Human Ortholog | |
Description | Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 12 | 4 | 4 | 9 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 4 | 1 | 8 | 6 | 8 | 14 | 6 | 10 | 16 | 16 | 0 | 2 | 0 | 2 | 6 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 102 | 99 | 276 | 184 | 225 | 283 | 303 | 331 | 216 | 214 | 198 | 230 | 60 | 102 | 103 | 297 | 354 | 251 | 97 | 107 | 225 | 76 | 111 | 138 |
Endoplasmic Reticulum | 0 | 4 | 7 | 2 | 14 | 20 | 40 | 57 | 50 | 48 | 30 | 72 | 9 | 10 | 3 | 11 | 14 | 11 | 0 | 1 | 26 | 23 | 58 | 57 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 1 | 1 | 0 | 0 | 6 | 13 | 3 | 20 | 13 | 21 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 1 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 0 | 7 | 22 | 10 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 3 | 2 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 3 | 1 | 6 | 1 | 12 |
Unique Cell Count | 102 | 101 | 283 | 188 | 237 | 305 | 353 | 411 | 273 | 285 | 260 | 332 | 69 | 111 | 105 | 300 | 364 | 254 | 101 | 120 | 264 | 112 | 186 | 221 |
Labelled Cell Count | 102 | 103 | 288 | 189 | 248 | 309 | 365 | 436 | 280 | 304 | 288 | 364 | 69 | 114 | 107 | 312 | 382 | 269 | 101 | 120 | 264 | 112 | 186 | 221 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 112.1 | 63.3 | 104.3 | 87.0 | 71.0 | 54.8 | 50.6 | 49.8 | 48.4 | 45.4 | 42.3 | 43.2 | 91.3 | 96.8 | 96.7 | 114.0 | 111.9 | 112.2 | 83.8 | 85.9 | 89.7 |
Std Deviation (1e-4) | 20.7 | 26.6 | 21.4 | 21.7 | 12.2 | 11.8 | 11.2 | 11.9 | 11.0 | 11.8 | 11.5 | 11.4 | 22.3 | 23.1 | 19.4 | 24.7 | 26.6 | 26.1 | 20.8 | 22.8 | 25.2 |
Intensity Change (Log2) | – | – | – | -0.26 | -0.56 | -0.93 | -1.04 | -1.07 | -1.11 | -1.2 | -1.3 | -1.27 | -0.19 | -0.11 | -0.11 | 0.13 | 0.1 | 0.11 | -0.32 | -0.28 | -0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 1.5 | 0 | 0.8 | 1.6 | 0 | 1.6 | 2.9 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.2 | -1.6 | -2.7 | -5.1 | -6.6 | -6.8 | -7.8 | -7.5 | -9.1 | -3.8 | -2.5 | 0.3 | 1.4 | -0.2 | 1.1 |
Endoplasmic Reticulum | 0 | 2.0 | 2.4 | 4.2 | 5.1 | 6.2 | 5.8 | 4.2 | 7.1 | 0 | 0 | 0 | 0.8 | 1.0 | 1.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 2.6 | 0 | 4.2 | 3.4 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0132 | 0.0009 | 0.0065 | 0.0015 | 0.0008 | 0.0024 | 0.0083 | 0.0005 | 0.0016 | 0.0004 | 0.0004 | 0.0009 | – | – | – | – | – | – |
Bud | 0.0044 | 0.001 | 0.0018 | 0.0028 | 0.0017 | 0.0016 | 0.0026 | 0.0009 | 0.0034 | 0.0034 | 0.0027 | 0.0015 | – | – | – | – | – | – |
Bud Neck | 0.0012 | 0.0007 | 0.0015 | 0.0022 | 0.0013 | 0.0013 | 0.0018 | 0.0008 | 0.0011 | 0.0018 | 0.0007 | 0.0014 | – | – | – | – | – | – |
Bud Periphery | 0.0054 | 0.0006 | 0.0013 | 0.0023 | 0.0006 | 0.0011 | 0.0047 | 0.0008 | 0.0067 | 0.0018 | 0.0013 | 0.0015 | – | – | – | – | – | – |
Bud Site | 0.0016 | 0.0008 | 0.0034 | 0.0053 | 0.0002 | 0.0006 | 0.0021 | 0.001 | 0.0011 | 0.0005 | 0.0006 | 0.0003 | – | – | – | – | – | – |
Cell Periphery | 0.0435 | 0.0163 | 0.0174 | 0.0208 | 0.0025 | 0.0328 | 0.0486 | 0.0197 | 0.0311 | 0.0064 | 0.011 | 0.0463 | – | – | – | – | – | – |
Cytoplasm | 0.5307 | 0.8409 | 0.6542 | 0.5334 | 0.7135 | 0.8526 | 0.4909 | 0.8404 | 0.7156 | 0.5707 | 0.6387 | 0.6508 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0177 | 0.0034 | 0.0126 | 0.0075 | 0.0067 | 0.0111 | 0.0303 | 0.0017 | 0.0093 | 0.0042 | 0.0136 | 0.0022 | – | – | – | – | – | – |
Eisosomes | 0.0004 | 0 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0007 | 0 | 0.0001 | 0 | 0.0001 | 0.0004 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.0544 | 0.0304 | 0.0348 | 0.014 | 0.0054 | 0.0062 | 0.0619 | 0.0245 | 0.0331 | 0.003 | 0.0022 | 0.0141 | – | – | – | – | – | – |
Endosome | 0.0215 | 0.0133 | 0.0351 | 0.0459 | 0.0192 | 0.0215 | 0.0184 | 0.0039 | 0.0089 | 0.0225 | 0.0112 | 0.0142 | – | – | – | – | – | – |
Golgi | 0.0052 | 0.0079 | 0.0101 | 0.0084 | 0.0017 | 0.0038 | 0.0047 | 0.0007 | 0.0017 | 0.0016 | 0.0008 | 0.0052 | – | – | – | – | – | – |
Lipid Particles | 0.0059 | 0.0012 | 0.0067 | 0.0064 | 0.0013 | 0.0041 | 0.01 | 0.0015 | 0.0024 | 0.0015 | 0.0047 | 0.008 | – | – | – | – | – | – |
Mitochondria | 0.007 | 0.0075 | 0.0058 | 0.0096 | 0.0009 | 0.002 | 0.0106 | 0.0006 | 0.0015 | 0.0006 | 0.0108 | 0.0133 | – | – | – | – | – | – |
None | 0.0078 | 0.0003 | 0.0212 | 0.0008 | 0.0004 | 0.0008 | 0.0127 | 0.0004 | 0.0093 | 0.0002 | 0.0127 | 0.0015 | – | – | – | – | – | – |
Nuclear Periphery | 0.0466 | 0.0037 | 0.0193 | 0.0084 | 0.0095 | 0.0104 | 0.0419 | 0.0055 | 0.0096 | 0.0079 | 0.0056 | 0.0239 | – | – | – | – | – | – |
Nucleolus | 0.0019 | 0.0001 | 0.0029 | 0.0008 | 0.0007 | 0.0019 | 0.0048 | 0.0006 | 0.0012 | 0.0007 | 0.0131 | 0.0029 | – | – | – | – | – | – |
Nucleus | 0.0107 | 0.0019 | 0.0047 | 0.0045 | 0.0065 | 0.0049 | 0.0111 | 0.0032 | 0.0038 | 0.0071 | 0.0117 | 0.0103 | – | – | – | – | – | – |
Peroxisomes | 0.0007 | 0.0001 | 0.0018 | 0.0015 | 0.0002 | 0.0003 | 0.0039 | 0.0001 | 0.0006 | 0.0001 | 0.0009 | 0.0001 | – | – | – | – | – | – |
Punctate Nuclear | 0.0056 | 0 | 0.0018 | 0.0002 | 0.0001 | 0.0002 | 0.002 | 0.0001 | 0.0004 | 0.0001 | 0.0005 | 0.0002 | – | – | – | – | – | – |
Vacuole | 0.1632 | 0.057 | 0.1268 | 0.2438 | 0.163 | 0.0314 | 0.1821 | 0.085 | 0.1332 | 0.2714 | 0.1928 | 0.1285 | – | – | – | – | – | – |
Vacuole Periphery | 0.0516 | 0.0118 | 0.0301 | 0.08 | 0.0638 | 0.0091 | 0.0461 | 0.0081 | 0.0244 | 0.0941 | 0.0639 | 0.0726 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 1980.3877 | 1970.8518 | 2232.4682 | 2019.7733 | 2129.3793 | 1874.1117 | 1732.8263 | 1636.7737 | 1690.4859 | 1663.5731 |
Translational Efficiency | 1.6351 | 1.673 | 1.5688 | 1.4677 | 1.7164 | 1.7596 | 1.687 | 1.8229 | 1.5909 | 1.8972 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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