Standard name
Human Ortholog
Description Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0.05 0.06 0.07 0.08 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 0 0 0 0.1 0.13 0.19 0.05 0.09 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.09 0.05 – 0.06 0 0 0 0 0 0.07 0.11 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0.11 0.09 0.05 0.14 – 0.08 0.18 0.18 0.22 0.21 0 0 0 0 0.14 0.07 0 0 0 0 0 0
Nucleus 0.17 0.28 0.16 0.08 0.07 0.07 – 0.06 0.05 0.06 0.07 0.07 0.11 0.07 0.05 0.09 0.19 0.09 0.16 0.16 0.06 0.05 0.06 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.86 0.83 0.93 0.86 0.8 0.79 – 0.78 0.75 0.72 0.75 0.75 0.83 0.79 0.76 0.88 0.75 0.82 0.69 0.7 0.59 0.61 0.57 0.56
Peroxisomes 0 0 0 0.05 0 0 – 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.15 0.15 0.25 0.37 0.46 0.45 – 0.5 0.48 0.5 0.44 0.51 0.24 0.31 0.33 0.18 0.09 0.23 0.11 0.07 0.26 0.24 0.22 0.28
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 2 5 3 8 – 12 11 21 20 7 0 0 0 0 1 1 0 2 3 3 4 7
Bud Neck 5 0 21 14 18 10 – 10 13 26 11 13 9 9 4 1 0 2 0 3 4 0 4 1
Bud Site 1 0 7 6 8 18 – 19 29 45 44 56 1 1 0 1 3 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 0 2 8 17 21 – 20 5 17 4 4 26 46 63 6 14 10 0 0 0 3 2 3
Endoplasmic Reticulum 0 0 1 1 0 1 – 0 0 0 0 0 0 0 0 0 2 2 0 0 0 1 3 0
Endosome 1 0 1 4 43 30 – 47 14 25 19 15 10 25 36 0 3 7 1 2 10 4 10 13
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Mitochondria 9 4 49 32 25 88 – 65 103 141 133 153 3 3 3 3 22 12 1 0 1 0 1 1
Nucleus 29 58 69 26 34 44 – 48 30 51 44 50 31 27 18 11 30 16 26 36 28 9 19 8
Nuclear Periphery 0 2 2 1 1 2 – 5 3 1 4 1 0 0 0 0 0 1 0 0 0 0 1 2
Nucleolus 144 172 405 297 366 494 – 607 420 575 462 546 225 286 257 109 119 141 115 160 258 119 179 177
Peroxisomes 0 2 3 19 18 27 – 49 30 23 21 28 2 4 2 1 0 2 0 0 1 0 1 1
SpindlePole 25 32 109 128 211 283 – 391 270 398 272 370 65 112 110 22 14 39 17 16 115 47 68 89
Vac/Vac Membrane 0 1 0 2 3 2 – 3 1 0 0 1 2 0 0 0 2 2 1 2 5 3 5 4
Unique Cell Count 168 207 434 346 458 629 779 558 803 614 727 270 360 336 124 159 173 168 229 435 198 315 319
Labelled Cell Count 218 271 671 543 747 1028 1277 929 1323 1034 1244 374 513 493 154 210 235 168 229 435 198 315 319


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 10.6 6.3 7.2 6.7 5.4 – 5.7 4.9 5.0 5.0 5.0 8.2 8.4 8.1 13.1 11.4 12.4 11.0 10.1 9.9
Std Deviation (1e-4) 1.8 2.4 1.7 2.6 1.8 1.7 – 1.9 1.6 1.5 1.4 1.5 2.3 2.3 2.6 4.2 4.0 4.0 5.7 2.6 2.5
Intensity Change (Log2) – – – 0.19 0.1 -0.21 – -0.14 -0.37 -0.33 -0.34 -0.33 0.38 0.41 0.37 1.06 0.86 0.98 0.8 0.68 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 1.7 0 2.7 3.1 0 0 0 0 0 0 0
Bud Neck -0.5 -0.7 -3.1 – -3.8 0 -1.4 -2.8 -3.0 -1.0 -1.7 -2.8 0 -2.8 -2.1
Bud Site 0.1 0.2 1.3 – 1.0 0 3.3 4.1 4.4 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 3.4 3.2 – 2.6 0 2.3 0 0 6.1 7.2 9.1 0 0 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 6.3 4.3 – 5.0 0 3.4 3.3 2.6 0 5.3 6.7 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.9 -3.2 1.3 – -1.7 0 2.9 4.4 4.2 -5.0 -5.9 -5.7 -3.0 0.8 -1.6
Nucleus -3.6 -4.0 -4.6 – -5.5 -5.5 -5.4 -4.5 -4.9 -1.6 -3.6 -4.6 -2.0 0.9 -2.1
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -3.5 -5.8 -6.6 – -6.9 0 -9.0 -7.6 -7.8 -4.2 -5.8 -6.7 -2.0 -6.2 -4.4
Peroxisomes 4.0 3.2 3.5 – 4.6 0 2.5 2.9 3.2 0 0 0 0 0 0
SpindlePole 3.6 6.5 6.6 – 8.5 0 8.3 6.4 8.6 -0.3 1.9 2.3 -1.7 -4.3 -0.7
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.77 4.5977 3.9915 3.5625 3.5682 3.638 2.9947 3.6514 3.0577 2.9128 2.8593 3.0455 5.169 5.8635 5.3698 5.1451 4.6019 5.3393
Actin 0.0032 0 0.0004 0.0001 0.0007 0.0002 0.006 0 0.0015 0.0019 0.029 0.004 0.0168 0 0.005 0.0002 0 0.0047
Bud 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0004 0 0.0001 0.0006 0.0003 0 0.0003 0 0.0012 0.0001 0.0001 0
Bud Neck 0.0014 0.0003 0.0007 0.0004 0.0005 0.0006 0.007 0.0016 0.0004 0.0021 0.0047 0.0006 0.0011 0.0006 0.0006 0.0025 0.0004 0.0007
Bud Periphery 0.0001 0.0002 0.0001 0 0.0003 0.0002 0.0008 0 0.0002 0.0007 0.0007 0.0001 0.0004 0.0001 0.001 0.0002 0.0001 0.0001
Bud Site 0.0045 0.0004 0.0015 0.0001 0.0004 0.0002 0.0047 0.0003 0.0019 0.0021 0.001 0.0002 0.0015 0.0003 0.0064 0.0007 0.0002 0.0002
Cell Periphery 0.0002 0.0001 0.0002 0 0.0001 0.0001 0.0005 0.0003 0.0002 0.0003 0.0008 0.0001 0.0003 0.0001 0.0002 0.0003 0.0001 0.0001
Cytoplasm 0.0009 0.0001 0.0009 0 0.0001 0.0001 0.0016 0 0.0005 0.0004 0.0009 0.0001 0.0016 0 0.001 0.0012 0 0.0001
Cytoplasmic Foci 0.0249 0.0005 0.0027 0.0022 0.0176 0.0069 0.0305 0.0009 0.0067 0.0106 0.0211 0.004 0.0328 0.0043 0.0045 0.0075 0.0014 0.0019
Eisosomes 0.0001 0 0 0 0.0001 0 0.0002 0 0 0 0.0006 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0004 0.0001 0.0005 0 0.0002 0.0002 0.0019 0 0.001 0.0001 0.0004 0.0002 0.0013 0 0.0006 0.0013 0 0.0004
Endosome 0.0123 0.0009 0.005 0.0057 0.0041 0.0062 0.0167 0.0003 0.0075 0.0169 0.0236 0.0013 0.0143 0.0004 0.0036 0.0288 0.0001 0.0021
Golgi 0.0053 0.0001 0.0013 0.0091 0.004 0.0032 0.0034 0.0001 0.0023 0.0086 0.0148 0.0011 0.0093 0.0001 0.001 0.0021 0.0001 0.001
Lipid Particles 0.0279 0.0033 0.0117 0.0199 0.0593 0.0131 0.0313 0.0025 0.0266 0.0241 0.0703 0.0142 0.0354 0.0048 0.0026 0.028 0.0019 0.0062
Mitochondria 0.0049 0.0003 0.0107 0.0035 0.0742 0.0032 0.0113 0 0.0075 0.0133 0.0183 0.0028 0.0021 0.0001 0.0011 0.0007 0.0006 0.0007
None 0.0008 0.0001 0.0004 0 0.0002 0.0001 0.0025 0 0.0002 0.0002 0.0172 0.0001 0.0032 0 0.0003 0.0004 0 0.0002
Nuclear Periphery 0.005 0.0029 0.0061 0.0006 0.0026 0.001 0.0144 0.0002 0.0029 0.0001 0.0057 0.0049 0.002 0 0.0076 0.0028 0.0002 0.0026
Nucleolus 0.7831 0.8979 0.8612 0.848 0.7313 0.8334 0.7399 0.878 0.8434 0.8008 0.5687 0.8294 0.7461 0.8578 0.8769 0.7375 0.7297 0.8325
Nucleus 0.0675 0.0841 0.0784 0.0786 0.0536 0.0577 0.059 0.0969 0.0762 0.1062 0.0506 0.0556 0.0682 0.1002 0.0735 0.1306 0.0714 0.0564
Peroxisomes 0.0136 0 0.0011 0.0006 0.0038 0.0011 0.0064 0.0001 0.0056 0.0033 0.0103 0.0011 0.0122 0.0002 0.0024 0.0009 0.0001 0.0003
Punctate Nuclear 0.042 0.0052 0.0143 0.0304 0.0447 0.0702 0.0531 0.017 0.0121 0.0044 0.1486 0.0797 0.0495 0.0303 0.0094 0.0517 0.1931 0.0894
Vacuole 0.0016 0.0024 0.0016 0.0002 0.0008 0.0009 0.0055 0.0016 0.0021 0.0022 0.0073 0.0003 0.001 0.0005 0.0009 0.002 0.0003 0.0002
Vacuole Periphery 0.0005 0.001 0.0011 0.0004 0.0014 0.0014 0.0028 0.0002 0.0008 0.0009 0.005 0.0003 0.0005 0.0001 0.0004 0.0007 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.6746 10.8563 5.7542 6.8581 8.9976 1.734 4.3123 7.9378 4.6959 5.2575
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan
Localization
Cell Percentages nucleolus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Tma23

Tma23


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tma23-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available