Standard name
Human Ortholog
Description Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.99 0.95 1.0 0.94 0.89 0.77 0.82 0.73 0.68 0.56 0.7 1.0 0.99 0.99 0.98 0.96 0.96 0.91 0.95 0.94 0.93 0.87 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0.08 0.1 0.1 0.3 0.48 0.38 0.5 0.56 0.72 0.52 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 4
Bud 1 2 5 0 4 1 2 3 1 4 0 5 1 1 1 1 0 0 0 0 0 0 3 6
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 3 0 0 0 3 1 1 0 0 1 1 1 5 3 8 11 7 0 0 0 0 0 1
Cytoplasm 224 237 113 41 126 149 123 187 80 86 92 109 472 510 515 558 570 494 223 230 111 227 272 305
Endoplasmic Reticulum 1 1 0 0 1 2 1 0 0 0 0 0 1 2 1 27 22 22 1 0 0 2 1 6
Endosome 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 4 7 3 0 0 2 6 5
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 2 1 2 1 0 0 1 0
Mitochondria 11 0 9 4 14 51 77 87 55 71 119 81 0 2 1 5 6 3 1 3 2 2 6 9
Nucleus 0 0 0 0 0 1 1 1 1 0 4 1 0 1 2 3 5 3 0 1 0 0 0 0
Nuclear Periphery 2 1 0 0 0 0 0 1 0 2 0 1 0 0 0 0 0 0 1 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 0 0 1 0 2 0 0 0 2 1 0 1 0 0 0 6 7
Vac/Vac Membrane 0 0 0 0 0 0 1 0 0 0 2 1 1 0 1 10 22 9 7 1 0 3 6 11
Unique Cell Count 233 240 119 41 134 168 160 227 109 126 165 156 473 516 520 569 593 512 245 243 118 245 312 365
Labelled Cell Count 243 245 129 45 145 207 207 281 139 164 219 201 476 524 524 616 644 546 245 243 118 245 312 365


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 6.1 4.6 4.3 4.2 3.6 3.0 3.4 3.4 3.5 3.1 3.2 6.1 5.8 5.8 5.8 7.0 6.4 5.0 4.8 5.6
Std Deviation (1e-4) 0.7 0.7 0.8 0.6 1.3 0.8 0.7 1.0 0.8 1.8 0.9 1.3 0.9 1.1 1.2 1.2 1.4 1.1 0.9 1.0 1.3
Intensity Change (Log2) – – – -0.1 -0.14 -0.38 -0.65 -0.45 -0.46 -0.42 -0.57 -0.55 0.39 0.33 0.31 0.31 0.58 0.47 0.1 0.05 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.5 -0.3 -1.9 -4.1 -3.3 -4.5 -5.3 -7.3 -5.2 4.4 2.8 3.1 2.0 0.6 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – 0.055 0.014 0.0432 0.0083 0.0137 0.0116 – – – – – –
Bud – – – – – – 0.002 0.0008 0.0009 0.0003 0.0012 0.0068 – – – – – –
Bud Neck – – – – – – 0.0116 0.0003 0.0005 0.0005 0.0008 0.0003 – – – – – –
Bud Periphery – – – – – – 0.0021 0.0005 0.0013 0.0002 0.0019 0.0061 – – – – – –
Bud Site – – – – – – 0.0057 0.0087 0.0082 0.0009 0.0038 0.0002 – – – – – –
Cell Periphery – – – – – – 0.0005 0.0003 0.001 0.0001 0.0004 0 – – – – – –
Cytoplasm – – – – – – 0.3779 0.6194 0.567 0.5929 0.4579 0.68 – – – – – –
Cytoplasmic Foci – – – – – – 0.0422 0.018 0.0173 0.0419 0.0435 0.0093 – – – – – –
Eisosomes – – – – – – 0.0003 0.0001 0.0003 0 0.0001 0 – – – – – –
Endoplasmic Reticulum – – – – – – 0.007 0.0035 0.0035 0.0025 0.0024 0.0013 – – – – – –
Endosome – – – – – – 0.0265 0.006 0.0219 0.0283 0.0361 0.0054 – – – – – –
Golgi – – – – – – 0.008 0.0055 0.0145 0.0131 0.023 0.0044 – – – – – –
Lipid Particles – – – – – – 0.0312 0.0006 0.0012 0.0066 0.0566 0.0026 – – – – – –
Mitochondria – – – – – – 0.0072 0.0007 0.0067 0.0012 0.0124 0.0024 – – – – – –
None – – – – – – 0.3736 0.3108 0.3012 0.2861 0.3289 0.2616 – – – – – –
Nuclear Periphery – – – – – – 0.0155 0.0009 0.0006 0.0017 0.001 0.0004 – – – – – –
Nucleolus – – – – – – 0.0009 0.0001 0.0001 0.0001 0.0002 0 – – – – – –
Nucleus – – – – – – 0.0079 0.001 0.0007 0.0015 0.0006 0.0009 – – – – – –
Peroxisomes – – – – – – 0.0091 0.0062 0.0062 0.0085 0.012 0.0055 – – – – – –
Punctate Nuclear – – – – – – 0.0084 0.0009 0.0004 0.0013 0.0013 0.0004 – – – – – –
Vacuole – – – – – – 0.0039 0.0014 0.0031 0.0034 0.0015 0.0006 – – – – – –
Vacuole Periphery – – – – – – 0.0034 0.0002 0.0003 0.0006 0.0007 0.0001 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.889 18.4786 13.7119 16.0096 15.2488 18.5224 20.9386 18.698 20.2013 16.7878
Translational Efficiency 1.4065 1.4617 1.3812 1.0335 1.1269 1.4083 1.3002 1.1707 0.968 1.1513

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (84%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ngl2

Ngl2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ngl2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available