ORF
Human Ortholog
Description Protein of unknown function; has strong similarity to alanyl-tRNA synthases from Eubacteria; null mutant displays decreased translation rate and increased readthrough of premature stop codons; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL040W is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0.05 0.06 0.1 0 0.07 0.08 0 0 0 0.06 0.05 0.06 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.97 0.93 0.86 0.8 0.68 0.64 0.66 0.58 0.56 0.57 0.99 0.99 0.99 0.94 0.96 0.93 0.96 0.92 0.95 0.98 0.97 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.09 0.14 0.26 0.31 0.28 0.3 0.41 0.46 0.38 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.09 0.13 0.1 0.1 0.11 0.11 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 3 5 10 6 5 5 0 0 1 0 0 0 0 0 2 0 0 1
Bud 0 1 3 2 8 7 14 5 21 16 22 19 0 0 0 1 0 1 1 1 1 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 13 9 33 10 15 25 37 41 75 27 53 63 9 12 24 10 10 11 0 0 0 0 0 0
Cytoplasm 625 318 594 313 416 471 527 454 510 483 402 460 577 412 589 149 194 170 615 310 589 338 513 574
Endoplasmic Reticulum 3 2 8 4 13 16 26 25 35 52 11 26 2 1 2 7 6 6 3 1 6 0 1 2
Endosome 0 0 1 0 1 0 0 0 0 0 0 2 1 0 2 1 0 2 5 3 4 0 0 3
Golgi 1 0 0 2 1 0 0 1 0 0 0 0 0 0 1 0 0 2 1 0 3 0 1 6
Mitochondria 5 2 5 30 67 152 241 197 230 346 332 301 0 1 1 2 5 3 2 2 4 0 1 5
Nucleus 2 6 0 0 6 2 4 7 4 7 4 3 2 1 0 2 7 2 2 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 3 3 12 0 5 3 2 0 0 0 0 0 1 1 0 2 0 1 0
Nucleolus 0 0 0 0 2 0 1 1 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0
Vac/Vac Membrane 3 0 11 10 13 16 66 91 78 81 79 91 1 2 1 7 1 4 2 9 2 2 2 1
Unique Cell Count 635 324 614 337 486 588 771 714 777 839 720 802 580 417 595 158 202 182 644 337 623 346 529 600
Labelled Cell Count 652 338 655 371 543 693 922 839 964 1026 911 972 592 431 621 179 223 202 644 337 623 346 529 600


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 9.8 8.8 8.3 8.2 7.8 7.6 7.7 7.8 7.1 6.7 7.3 9.8 10.1 9.4 9.5 8.5 9.4 11.1 11.4 11.9
Std Deviation (1e-4) 1.1 1.9 1.3 1.3 1.5 1.6 1.6 1.6 1.5 1.5 1.5 1.6 1.4 1.3 1.4 1.7 1.5 1.8 1.7 1.7 1.9
Intensity Change (Log2) – – – -0.07 -0.1 -0.17 -0.2 -0.18 -0.17 -0.3 -0.38 -0.27 0.16 0.21 0.11 0.12 -0.04 0.1 0.34 0.39 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 2.2 0 3.1 2.4 3.4 2.8 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.7 -1.8 -0.9 -0.5 0.3 3.0 -2.0 1.5 1.8 -3.6 -1.9 -1.1 0.5 -0.2 0.3
Cytoplasm -2.7 -6.7 -9.1 -13.4 -14.8 -14.2 -16.8 -17.1 -16.8 3.4 2.1 2.7 -1.4 -0.5 -2.0
Endoplasmic Reticulum 0 1.7 1.8 2.5 2.6 3.4 4.6 0.3 2.4 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 6.3 8.6 12.9 14.7 13.5 14.2 17.8 19.0 16.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 3.2 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.2 1.0 1.1 5.5 7.5 6.2 6.1 6.7 6.9 -2.8 -1.9 -2.8 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.3697 5.9211 6.3544 5.3927 5.0311 6.0363 5.0385 5.9576 5.7665 4.2842 5.0694 5.3945 3.773 5.4698 4.8086 4.2551 3.82 4.9156
Actin 0.0177 0.0003 0.0011 0.0014 0.0001 0.0003 0.0046 0.0003 0.0013 0.0016 0.0025 0.0021 0.0007 0.0002 0.0007 0.0006 0.0003 0.0003
Bud 0.0002 0.0003 0.0003 0.0006 0.0002 0.0001 0.0002 0.0004 0.002 0.0019 0.0007 0.0007 0.0001 0.0016 0.0004 0.0002 0.0002 0.0018
Bud Neck 0.0005 0.0009 0.0002 0.0005 0.0003 0.0006 0.0016 0.0003 0.0004 0.0013 0.0005 0.0016 0.0001 0.0001 0.0002 0.0003 0.0003 0.0007
Bud Periphery 0.0002 0.0001 0.0003 0.0003 0.0001 0.0001 0.0002 0.0003 0.0007 0.0015 0.0005 0.0002 0.0001 0.0006 0.0002 0.0001 0.0001 0.0002
Bud Site 0.0015 0.0037 0.0006 0.0006 0.0001 0.0001 0.0007 0.0084 0.001 0.0069 0.0008 0.0002 0.0002 0.0008 0.0008 0.0001 0.0001 0.0001
Cell Periphery 0.0004 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.8139 0.8827 0.904 0.9198 0.941 0.9234 0.8935 0.9533 0.957 0.8965 0.8911 0.937 0.9049 0.9654 0.9374 0.9593 0.9243 0.9514
Cytoplasmic Foci 0.0132 0.0089 0.0063 0.0088 0.0131 0.0054 0.0121 0.0056 0.0069 0.0212 0.0175 0.0092 0.0083 0.0039 0.0069 0.0075 0.01 0.006
Eisosomes 0.0001 0 0 0 0 0 0 0 0 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0093 0.0012 0.0052 0.0094 0.0018 0.0052 0.0091 0.005 0.0026 0.0027 0.0044 0.0036 0.0091 0.0015 0.0031 0.005 0.0034 0.0048
Endosome 0.004 0.0068 0.005 0.0165 0.0043 0.0035 0.0149 0.0035 0.0063 0.0172 0.0273 0.0064 0.0101 0.0024 0.0054 0.0094 0.0165 0.0047
Golgi 0.002 0.0006 0.001 0.0012 0.0002 0.0003 0.0015 0.0003 0.0009 0.002 0.0019 0.0006 0.0006 0.0005 0.0005 0.0005 0.0008 0.0003
Lipid Particles 0.0008 0.0002 0.0003 0.0005 0 0.0001 0.0022 0.0001 0.0003 0.0171 0.0028 0.0017 0.0005 0 0.0001 0.0001 0.0001 0
Mitochondria 0.0003 0.0002 0.0004 0.0004 0.0001 0.0003 0.0008 0.0003 0.0006 0.0013 0.0011 0.002 0.0002 0.0062 0.0002 0.0003 0.0003 0.0008
None 0.1309 0.084 0.0722 0.0299 0.0338 0.0579 0.0359 0.0177 0.0132 0.0057 0.0185 0.027 0.0599 0.0142 0.0405 0.0128 0.0401 0.0257
Nuclear Periphery 0.0009 0.0005 0.0006 0.0025 0.0005 0.0007 0.0024 0.0007 0.0008 0.0012 0.0127 0.0022 0.0012 0.0003 0.0007 0.0007 0.0005 0.0004
Nucleolus 0.0001 0.0004 0 0.0001 0 0 0.0003 0 0.0001 0.0003 0.0005 0.0002 0 0 0 0 0 0.0001
Nucleus 0.0008 0.003 0.0007 0.0021 0.0015 0.0011 0.0105 0.0016 0.0025 0.0028 0.0038 0.0019 0.0009 0.0009 0.0009 0.0009 0.0007 0.0016
Peroxisomes 0.0004 0.0002 0.0001 0.0001 0 0 0.0008 0.0001 0.0003 0.0115 0.0011 0.0007 0.0001 0 0.0001 0.0001 0.0001 0.0001
Punctate Nuclear 0.0014 0.0033 0.0002 0.0007 0.0004 0.0002 0.0058 0.0002 0.0005 0.0013 0.0073 0.001 0.0003 0.0001 0.0003 0.0002 0.0001 0.0002
Vacuole 0.001 0.0026 0.0012 0.004 0.0021 0.0007 0.0025 0.0015 0.0021 0.005 0.0036 0.0013 0.0023 0.0009 0.0011 0.0014 0.0017 0.0007
Vacuole Periphery 0.0001 0.0002 0.0002 0.0005 0.0002 0.0001 0.0004 0.0002 0.0003 0.0008 0.0011 0.0004 0.0003 0.0002 0.0002 0.0004 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.9614 21.5837 15.4822 16.3411 17.7392 16.0874 24.0056 21.6029 23.4501 27.5127
Translational Efficiency 0.6618 0.6666 0.8942 0.9483 0.8186 1.4692 0.915 1.0094 0.9245 0.8077

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
ORF
Human Ortholog
Description Protein of unknown function; has strong similarity to alanyl-tRNA synthases from Eubacteria; null mutant displays decreased translation rate and increased readthrough of premature stop codons; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL040W is not an essential gene
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

YNL040W

YNL040W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ynl040w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available