Standard name | |
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Human Ortholog | |
Description | Putative protein tyrosine phosphatase; required for cell cycle arrest in response to oxidative damage of DNA |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 2 | 0 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 6 |
Bud | 0 | 3 | 0 | 1 | 9 | 17 | 12 | 20 | 12 | 11 | 17 | 24 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 1 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 1 | 1 | 3 | 9 | 4 | 8 | 10 | 11 | 11 | 19 | 4 | 12 | 16 | 2 | 10 | 10 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 46 | 263 | 211 | 343 | 569 | 525 | 561 | 600 | 439 | 513 | 313 | 521 | 483 | 556 | 741 | 87 | 96 | 134 | 77 | 258 | 200 | 299 | 329 | 448 |
Endoplasmic Reticulum | 0 | 4 | 1 | 0 | 2 | 6 | 7 | 14 | 9 | 7 | 7 | 18 | 4 | 6 | 4 | 7 | 5 | 8 | 0 | 1 | 4 | 12 | 13 | 28 |
Endosome | 0 | 0 | 0 | 0 | 4 | 2 | 0 | 7 | 0 | 3 | 1 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 1 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 3 | 4 |
Mitochondria | 5 | 8 | 9 | 37 | 118 | 245 | 436 | 432 | 363 | 497 | 337 | 596 | 0 | 0 | 1 | 4 | 2 | 3 | 1 | 6 | 4 | 10 | 12 | 16 |
Nucleus | 0 | 1 | 0 | 0 | 3 | 0 | 2 | 7 | 0 | 2 | 1 | 5 | 2 | 0 | 2 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 6 | 0 | 3 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 0 | 2 | 2 | 5 | 3 | 28 | 22 | 43 | 15 | 23 | 4 | 2 | 2 | 2 | 0 | 1 | 0 | 4 | 1 | 22 | 19 | 27 |
Unique Cell Count | 47 | 273 | 216 | 351 | 604 | 637 | 784 | 927 | 644 | 882 | 543 | 952 | 489 | 566 | 750 | 89 | 99 | 141 | 84 | 280 | 220 | 358 | 396 | 558 |
Labelled Cell Count | 52 | 282 | 222 | 384 | 710 | 809 | 1028 | 1126 | 855 | 1092 | 710 | 1213 | 500 | 582 | 773 | 104 | 115 | 158 | 84 | 280 | 220 | 358 | 396 | 558 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 6.0 | 4.9 | 5.0 | 4.8 | 4.8 | 4.1 | 4.6 | 4.4 | 4.4 | 4.8 | 4.6 | 7.2 | 7.1 | 7.2 | 7.4 | 7.7 | 7.7 | 7.7 | 7.8 | 8.1 |
Std Deviation (1e-4) | 0.5 | 1.1 | 0.6 | 0.8 | 0.9 | 1.0 | 0.9 | 1.0 | 0.9 | 0.8 | 0.9 | 0.8 | 0.9 | 0.9 | 0.9 | 1.3 | 1.3 | 1.2 | 1.1 | 1.4 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.04 | -0.02 | -0.03 | -0.25 | -0.07 | -0.14 | -0.15 | -0.03 | -0.08 | 0.57 | 0.56 | 0.56 | 0.62 | 0.66 | 0.68 | 0.66 | 0.69 | 0.74 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | -2.0 | -5.6 | -8.1 | -9.6 | -8.7 | -11.0 | -10.8 | -11.8 | 1.1 | 0.5 | 1.2 | 0 | -0.4 | -1.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 2.6 | 2.8 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.47 | 4.4665 | 4.0747 | 3.7315 | 3.3589 | 4.0435 | 2.9832 | 4.2948 | 3.7693 | 3.2 | 3.769 | 4.0637 | 1.2815 | 2.4381 | 2.2842 | 1.8956 | 1.943 | 1.9234 |
Actin | 0.0005 | 0.0007 | 0.0008 | 0.0003 | 0.0001 | 0.0004 | 0.0215 | 0.0037 | 0.0009 | 0.0001 | 0.0011 | 0.0016 | 0.0056 | 0.0026 | 0.0014 | 0.004 | 0.0018 | 0.0033 |
Bud | 0.0028 | 0.0037 | 0.0048 | 0.0007 | 0.0008 | 0.0034 | 0.0006 | 0.0008 | 0.0008 | 0.0009 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0012 | 0.001 | 0.0006 | 0.0001 |
Bud Neck | 0.0012 | 0.0004 | 0.0004 | 0.0017 | 0.0005 | 0.001 | 0.0012 | 0.0002 | 0.0014 | 0.0006 | 0.0004 | 0.0006 | 0.0008 | 0.0008 | 0.0003 | 0.0006 | 0.0009 | 0.0009 |
Bud Periphery | 0.0013 | 0.0019 | 0.0019 | 0.0004 | 0.001 | 0.0008 | 0.0005 | 0.0005 | 0.0009 | 0.0005 | 0.0001 | 0.0005 | 0.0002 | 0 | 0.001 | 0.0053 | 0.0006 | 0.0001 |
Bud Site | 0.0117 | 0.0073 | 0.0069 | 0.0021 | 0.0002 | 0.0005 | 0.0061 | 0.0024 | 0.0047 | 0.0011 | 0.0008 | 0.0002 | 0.0044 | 0.0048 | 0.0048 | 0.0008 | 0.0017 | 0.0006 |
Cell Periphery | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0 |
Cytoplasm | 0.8073 | 0.8693 | 0.851 | 0.8464 | 0.7691 | 0.8261 | 0.7708 | 0.9056 | 0.8614 | 0.8558 | 0.7397 | 0.87 | 0.8079 | 0.9321 | 0.918 | 0.7805 | 0.7046 | 0.8692 |
Cytoplasmic Foci | 0.0272 | 0.0237 | 0.0231 | 0.0314 | 0.0327 | 0.0312 | 0.0325 | 0.019 | 0.0162 | 0.0314 | 0.0259 | 0.0176 | 0.0518 | 0.0129 | 0.0125 | 0.0247 | 0.0279 | 0.0478 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0094 | 0.0032 | 0.0021 | 0.0027 | 0.0051 | 0.0046 | 0.0051 | 0.0021 | 0.004 | 0.0025 | 0.003 | 0.0025 | 0.0049 | 0.0022 | 0.0018 | 0.0067 | 0.0011 | 0.0023 |
Endosome | 0.0303 | 0.0268 | 0.0263 | 0.0486 | 0.0381 | 0.04 | 0.0275 | 0.0183 | 0.018 | 0.036 | 0.0475 | 0.0166 | 0.0333 | 0.0093 | 0.0198 | 0.0597 | 0.0568 | 0.0343 |
Golgi | 0.0026 | 0.002 | 0.0016 | 0.0012 | 0.0015 | 0.0015 | 0.0084 | 0.0029 | 0.0018 | 0.0011 | 0.0023 | 0.0029 | 0.0071 | 0.0008 | 0.0011 | 0.01 | 0.0624 | 0.0096 |
Lipid Particles | 0.001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0043 | 0.0001 | 0.0004 | 0.0002 | 0.003 | 0.0017 | 0.0048 | 0.0001 | 0.0002 | 0.0298 | 0.0297 | 0.0047 |
Mitochondria | 0.0022 | 0.001 | 0.0017 | 0.0008 | 0.0044 | 0.0017 | 0.0033 | 0.0006 | 0.0027 | 0.0005 | 0.0009 | 0.0034 | 0.0019 | 0.0079 | 0.0041 | 0.0197 | 0.0755 | 0.007 |
None | 0.091 | 0.0482 | 0.0677 | 0.0409 | 0.1356 | 0.0761 | 0.1014 | 0.038 | 0.0784 | 0.0545 | 0.1399 | 0.0763 | 0.06 | 0.0229 | 0.0235 | 0.0365 | 0.0095 | 0.0063 |
Nuclear Periphery | 0.0009 | 0.0007 | 0.0006 | 0.0012 | 0.001 | 0.0006 | 0.0008 | 0.0005 | 0.0009 | 0.0007 | 0.001 | 0.0002 | 0.0009 | 0.0004 | 0.0008 | 0.0022 | 0.0005 | 0.0004 |
Nucleolus | 0.0005 | 0.0002 | 0.0006 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0004 | 0 | 0.0003 | 0.0002 | 0.0022 | 0.0001 |
Nucleus | 0.0021 | 0.003 | 0.0028 | 0.0025 | 0.0021 | 0.0026 | 0.0009 | 0.0009 | 0.001 | 0.0012 | 0.0022 | 0.0005 | 0.0011 | 0.0005 | 0.0017 | 0.0013 | 0.0012 | 0.0006 |
Peroxisomes | 0.0002 | 0.0002 | 0.0004 | 0.0003 | 0.0004 | 0.0002 | 0.0055 | 0.0002 | 0.0002 | 0.0002 | 0.0258 | 0.0021 | 0.0055 | 0.0002 | 0.0002 | 0.0052 | 0.0105 | 0.0072 |
Punctate Nuclear | 0.0015 | 0.0007 | 0.0009 | 0.002 | 0.0005 | 0.0021 | 0.0041 | 0.0002 | 0.0003 | 0.0003 | 0.0016 | 0.0002 | 0.0031 | 0.0001 | 0.0003 | 0.0005 | 0.0005 | 0.0032 |
Vacuole | 0.0049 | 0.0057 | 0.0051 | 0.0134 | 0.0053 | 0.0056 | 0.004 | 0.0032 | 0.0046 | 0.0107 | 0.0033 | 0.002 | 0.005 | 0.0019 | 0.0053 | 0.0083 | 0.0092 | 0.0016 |
Vacuole Periphery | 0.0009 | 0.001 | 0.0009 | 0.0028 | 0.0014 | 0.0009 | 0.0006 | 0.0006 | 0.0007 | 0.0017 | 0.0007 | 0.0005 | 0.0008 | 0.0003 | 0.0011 | 0.0026 | 0.0024 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 43.0897 | 49.5252 | 37.7706 | 26.8912 | 41.9008 | 52.2943 | 45.9019 | 47.4099 | 37.7339 | 37.8811 |
Translational Efficiency | 1.1119 | 0.8734 | 0.8852 | 1.1847 | 0.9828 | 0.9451 | 0.8864 | 1.008 | 0.9255 | 1.2247 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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