Standard name
Human Ortholog
Description Cholinephosphotransferase; required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription; CPT1 has a paralog, EPT1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.07 0.05 0.11 0.09 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.07 0.06 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.26 0.67 0.58 0.73 0.8 0.81 0.72 0.61 0.75 0.69 0.71 0.7 0.5 0.44 0.63 0.82 0.66 0.68 0 0.1 0 0 0 0
Endoplasmic Reticulum 0.37 0.15 0.25 0.13 0.1 0.07 0.11 0.11 0.08 0.09 0.08 0.12 0.45 0.55 0.38 0 0 0 0.42 0.18 0.46 0.44 0.57 0.51
Endosome 0.05 0 0 0.06 0 0.07 0.07 0.13 0.1 0.12 0.1 0.06 0 0 0 0 0.08 0.07 0 0 0.08 0.06 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.42 0.24 0.23 0.12 0.13 0.07 0.11 0.12 0.07 0.05 0 0.06 0.06 0 0 0.2 0.31 0.35 0.47 0.64 0.37 0.38 0.32 0.4
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 6 11 4 16 17 50 24 55 32 34 34 22 20 1 6 1 1 0 2 1 1 3
Bud 0 0 0 3 1 4 11 23 32 30 33 18 4 5 3 0 1 0 0 0 1 0 1 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 1 0 2 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 0 0 6 2 6 11 10 12 13 19 0 0 0 0 0 0 0 0 0
Cytoplasm 5 37 167 211 322 295 383 417 330 344 246 315 455 423 695 77 117 101 0 10 7 8 16 17
Endoplasmic Reticulum 7 8 72 38 42 24 59 75 34 46 27 53 412 531 421 1 3 1 24 19 163 164 331 333
Endosome 1 0 10 16 15 25 37 87 42 58 34 29 15 10 4 1 15 10 1 3 27 22 24 13
Golgi 0 0 0 0 0 0 0 3 0 0 0 2 0 0 0 0 1 1 0 1 11 18 11 7
Mitochondria 0 0 3 0 2 1 6 10 7 10 17 19 0 0 0 4 0 2 0 2 7 6 5 6
Nucleus 0 1 0 1 0 0 0 4 1 0 0 0 1 2 2 0 4 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 6 1 0 0 1 0 0 0 0 0 0 0 0 2 1 1 1
Nucleolus 0 0 0 0 0 1 1 8 0 4 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 3 1 4 0 2 4 3 3 1 0 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 8 13 66 34 51 26 61 83 31 25 15 25 59 30 41 19 55 52 28 68 131 142 185 267
Unique Cell Count 19 55 288 288 401 363 533 682 442 499 348 452 915 959 1108 94 177 148 60 107 358 370 583 660
Labelled Cell Count 21 59 324 316 437 395 577 777 505 582 421 514 995 1037 1205 103 203 168 60 107 358 370 583 660


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.8 8.9 9.6 10.0 10.3 9.4 8.8 9.0 8.8 8.4 8.3 8.1 11.4 11.0 11.0 8.0 9.3 11.2 12.2 12.6 13.2
Std Deviation (1e-4) 1.9 1.5 2.0 1.9 1.8 1.8 1.7 1.6 1.6 1.5 1.5 1.5 1.7 1.5 1.4 1.7 1.6 2.3 1.7 2.0 2.0
Intensity Change (Log2) – – – 0.06 0.1 -0.04 -0.13 -0.08 -0.13 -0.19 -0.21 -0.24 0.25 0.2 0.19 -0.27 -0.05 0.22 0.35 0.4 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.2 0 1.6 0.9 3.2 2.2 4.5 3.8 3.2 1.3 0.2 -0.3 0 0 0
Bud 0 0 0 0 3.2 4.7 4.2 5.4 3.4 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.9 6.4 6.5 4.0 0.9 4.7 3.1 3.3 3.3 -2.4 -4.1 1.5 4.2 1.7 2.1
Endoplasmic Reticulum -3.6 -5.1 -6.6 -5.2 -5.6 -6.5 -6.0 -6.0 -4.7 6.0 9.0 4.1 -5.1 -6.6 -6.4
Endosome 1.2 0.2 1.9 2.0 4.4 3.1 3.9 3.1 1.7 -1.9 0 0 0 2.3 1.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 2.8 2.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.5 -3.5 -5.7 -4.3 -4.2 -6.2 -7.6 -7.0 -7.0 -8.0 -11.0 -10.9 -0.5 1.9 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.1217 6.0019 6.1862 5.7813 5.3501 5.8655 4.3023 6.2906 5.3485 4.7439 4.3015 5.1997 3.0067 5.0807 3.9805 3.7682 3.3559 3.8959
Actin 0.0248 0.0003 0.0105 0.0005 0.0019 0.0068 0.0038 0.0006 0.0111 0.0002 0.0032 0.0001 0.0058 0.0174 0.0027 0.0001 0.0001 0.0001
Bud 0.0007 0.0012 0.0021 0.0004 0.0004 0.0002 0.0002 0.0001 0.0003 0.0002 0.0004 0.0001 0.0005 0.0002 0.0003 0.0002 0.0003 0.0001
Bud Neck 0.0038 0.0006 0.0009 0.0004 0.0013 0.0022 0.0002 0.0003 0.0009 0.0003 0.0006 0.0005 0.0009 0.0004 0.0008 0.0007 0.0002 0.0016
Bud Periphery 0.0011 0.0005 0.0019 0.0004 0.0009 0.0003 0.0003 0.0003 0.0005 0.0005 0.0007 0.0002 0.0006 0.0004 0.0006 0.0002 0.0003 0.0002
Bud Site 0.002 0.009 0.0031 0.0005 0.0006 0.0008 0.0005 0.0005 0.0019 0.0002 0.0026 0.0001 0.0014 0.0005 0.0007 0.0001 0.0001 0.0001
Cell Periphery 0.0018 0.0012 0.0009 0.0005 0.0004 0.0003 0.0008 0.0007 0.0007 0.0006 0.0008 0.0005 0.0009 0.0015 0.0019 0.0004 0.0002 0.0004
Cytoplasm 0.2238 0.3377 0.3417 0.3004 0.1283 0.2764 0.1358 0.2414 0.1586 0.1374 0.1473 0.1819 0.122 0.1724 0.157 0.1055 0.0528 0.1522
Cytoplasmic Foci 0.0381 0.0159 0.0283 0.017 0.0353 0.0166 0.0244 0.0158 0.0231 0.006 0.0256 0.0097 0.024 0.0151 0.0077 0.0048 0.0191 0.0095
Eisosomes 0.0003 0 0.0002 0 0 0 0.0001 0 0.0001 0 0.0001 0 0.0005 0 0.0004 0 0 0
Endoplasmic Reticulum 0.2811 0.257 0.2445 0.1519 0.233 0.2619 0.3651 0.362 0.365 0.3479 0.2389 0.3179 0.3513 0.4088 0.3671 0.2304 0.1804 0.4115
Endosome 0.1363 0.1111 0.1391 0.1542 0.2799 0.154 0.1981 0.1618 0.2235 0.2049 0.2548 0.2007 0.2097 0.1158 0.1603 0.1834 0.2121 0.1439
Golgi 0.0106 0.0029 0.0046 0.003 0.005 0.0045 0.018 0.0096 0.0154 0.0047 0.0109 0.0072 0.0176 0.0105 0.0062 0.0019 0.0797 0.0033
Lipid Particles 0.013 0.0044 0.0068 0.0072 0.006 0.0042 0.0341 0.0078 0.0136 0.0027 0.0329 0.0052 0.0279 0.0077 0.0076 0.0007 0.0033 0.0027
Mitochondria 0.0078 0.001 0.0052 0.0016 0.0047 0.0015 0.0141 0.0132 0.0045 0.0029 0.0625 0.0043 0.0213 0.0016 0.0041 0.0009 0.0029 0.0019
None 0.0258 0.0102 0.0169 0.0142 0.0019 0.0034 0.0051 0.0037 0.0088 0.001 0.0009 0.0051 0.0523 0.0006 0.0263 0.0004 0.0009 0.0046
Nuclear Periphery 0.0477 0.0309 0.028 0.0465 0.0946 0.0472 0.0382 0.037 0.0353 0.0512 0.0262 0.0422 0.04 0.0343 0.0457 0.0854 0.0514 0.0556
Nucleolus 0.001 0.0003 0.0032 0.0002 0.0007 0.0003 0.0001 0.0001 0.0002 0.0001 0.0003 0.0001 0.0008 0.0001 0.0003 0.0001 0.0001 0.0001
Nucleus 0.0094 0.0092 0.0077 0.0069 0.0155 0.0096 0.0027 0.003 0.0026 0.0038 0.0045 0.0034 0.004 0.0038 0.0048 0.0062 0.0029 0.0079
Peroxisomes 0.0108 0.0002 0.0012 0.0005 0.0004 0.0004 0.0061 0.0003 0.0068 0 0.005 0.0001 0.0063 0.0017 0.0002 0 0.0001 0.0001
Punctate Nuclear 0.0066 0.001 0.0058 0.004 0.0036 0.0021 0.0084 0.0005 0.0006 0.0002 0.0014 0.0002 0.0038 0.0005 0.0008 0.0002 0.0001 0.0002
Vacuole 0.1415 0.1957 0.1348 0.2747 0.1522 0.19 0.1295 0.1288 0.1076 0.1956 0.1524 0.1926 0.099 0.1952 0.1854 0.3295 0.344 0.1779
Vacuole Periphery 0.0119 0.0095 0.0127 0.015 0.0334 0.0172 0.0145 0.0124 0.0187 0.0396 0.0279 0.0278 0.0094 0.0118 0.019 0.0488 0.049 0.0262

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 85.8826 40.0559 45.8233 61.936 56.8856 60.6443 56.7428 62.6429 58.8584 92.113
Translational Efficiency 0.7994 1.1965 0.8142 0.6552 0.9316 1.4176 0.8506 0.6796 0.9144 0.7059

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cholinephosphotransferase; required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription; CPT1 has a paralog, EPT1, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (34%), ER (9%), mixed (44%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cpt1

Cpt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cpt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available